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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳逸聰(Yit-Tsong Chen) | |
dc.contributor.author | Chia-Wei Hsu | en |
dc.contributor.author | 許家維 | zh_TW |
dc.date.accessioned | 2021-06-16T13:25:27Z | - |
dc.date.available | 2016-07-30 | |
dc.date.copyright | 2013-07-30 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-07-23 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62057 | - |
dc.description.abstract | 肝細胞癌(Hepatocellular carcinoma)是肝癌中最常見的一種,造成原因有病毒性肝炎(Viral hepatitis)、酒精性肝炎(Alcoholic hepatitis)、肝硬化(Cirrhosis)等等。肝細胞癌是台灣癌症中致死率最高的,主要原因為不容易早期發現,等到發現時常常已經到了癌症末期。因此研發早期偵測肝細胞癌的方法,可以降低病人的死亡率。肝細胞癌有兩種生物標記(Biomarker),甲種胎兒蛋白(Alpha-fetoprotein)與
岩藻糖水解酶(Alpha-L-fucosidase)。先前研究發現,甲種胎兒蛋白與岩藻糖水解酶在肝細胞癌患者的血清中濃度與正常人不同。所以檢測甲種胎兒蛋白與岩藻糖水解酶在血清中濃度,可以於早期判斷患者是否罹患肝細胞癌。本論文使用矽奈米線場效電晶體(Silicon nanowire field-effect transistors)來偵測岩藻糖水解酶與Fuconojirimycin衍生物之間的結合常數,並且利用分析方法來得到岩藻糖水解酶在血清中濃度。 矽奈米線場效電晶體具有即時回應(Real-time)、非標定偵測(Label-free detection)、高靈敏度(High sensitivity)與專一選擇性(Selectivity)等優點,在生醫檢測上受到相當大的重視。Fuconojirimycin衍生物是岩藻糖水解酶的抑制劑(Inhibitor)之一,可以與岩藻糖水解酶緊密結合,因此我們將Fuconojirimycin衍生物修飾在矽奈米線場效電晶體表面。前半部分實驗我們將含有不同濃度的岩藻糖水解酶溶液流過矽奈米線場效電晶體表面,根據不同濃度下電訊號變化量得到岩藻糖水解酶與Fuconojirimycin衍生物的結合常數(Binding constant)。後半部分實驗我們將病人血清稀釋,接著利用標準添加法(Standard addition method)加入定量的岩藻糖水解酶並進行偵測,最後從檢量線(Fitting curve)推算出病人血清中岩藻糖水解酶的濃度。跟傳統判斷蛋白質濃度方法,例如螢光法相較,矽奈米線場效電晶體縮短了偵測時間,且不需純化分離岩藻糖水解酶。我們的研究給予了早期檢測肝細胞癌一個可靠且快速的新方法。 | zh_TW |
dc.description.abstract | Hepatocellular carcinoma (HCC), one of the most common malignant neoplasms, is closely associated with cirrhosis, but so far with very poor prognosis. Recent clinical studies revealed that alpha-fetoprotein (AFP) and alpha-L-fucosidase (AFU) enzyme play important roles in the early HCC diagnosis, where the simultaneous determination of both activities can drastically increase diagnostic accuracy and sensitivity. In this study, we applied silicon nanowire field-effect transistors (SiNW-FETs) as a sensitive biosensor for the early HCC diagnosis to detect AFU and AFP. While AFU can be probed by a fuconojirimycin derivative (i.e., 1-aminomethyl-FNJ, the AFU inhibitor), AFP is detected with AFP antibody. To detect AFU, we modified FNJ as a receptor on the surface of a SiNW-FET. Taking advantage of the real-time, label-free, high sensitivity and selectivity of SiNW-FET, we determined the binding affinity between AFU and FNJ and measured the concentrations of AFU in serum. We can also determined concentration of AFU in human serum by using an analytical method. In previous researches, HCC patients have abnormal concentration of AFU; therefore SiNW-FET can be used to estimate if a patient has HCC. Compare to traditional methods, such as fluorescence method, SiNW-FET shorten the diagnosis time and has no need to purify AFU. Our results give a great choice in early-stage diagnosis of HCC. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T13:25:27Z (GMT). No. of bitstreams: 1 ntu-102-R00223151-1.pdf: 5318570 bytes, checksum: 96a328ac6d04e71d14dba5b8685b0d18 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vii Chapter 1 導論 1 1.1 肝細胞癌之檢測 1 1.2 岩藻糖水解酶與抑制劑 4 1.2.1 岩藻糖水解酶 4 1.2.2 岩藻糖水解酶的抑制劑 6 1.2.3 岩藻糖水解酶濃度標定 8 1.3 矽奈米線場效電晶體 9 1.3.1 矽奈米線場效電晶體用作生物感測器 9 1.3.2 矽奈米線場效電晶體之製備 10 1.3.3 晶片修飾 13 Chapter 2 實驗方法 14 2.1 場效電晶體製成 15 2.1.1 SOI場效電晶體 15 2.1.2 Multiple wire FET 場效電晶體 17 2.2 表面修飾 19 2.2.1 FNJ修飾方法 19 2.2.2 修飾策略 21 2.2.3 FNJ的衍生物修飾方式 23 2.3 微流體通道裝置 25 2.4 實驗儀器 26 2.5 TmF、HuF、FNJ與血清準備 27 2.5.1 TmF、HuF準備 27 2.5.2 FNJ製備 30 2.5.3 血清準備 34 2.6 緩衝溶液選擇 35 2.7 實驗步驟 37 2.7.1 架設系統 37 2.7.2 樣品量測 38 Chapter 3 實驗結果與討論 39 3.1 修飾證明 39 3.1.1 ICPTES修飾 39 3.1.2 APTMS修飾 40 3.2 蘭米爾吸附模型 43 3.3 TmF電訊號量測 46 3.4 HuF電訊號量測 48 3.5 人體血清電訊號量測 51 3.5.1 標準添加法 52 3.5.2 實驗結果 54 3.6 以FNJ衍生物量測TmF電訊號 56 3.7 牛血清白蛋白電訊號量測 60 Chapter 4 結論 62 REFERENCE 63 | |
dc.language.iso | zh-TW | |
dc.title | 以矽奈米線場效電晶體作為肝細胞癌生物檢測器 | zh_TW |
dc.title | Using Silicon Nanowires Field Effect Transistor as biosensors for Hepatocellular Carcinoma Diagnosis | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林俊宏(Chun-Hung Lin),潘建源(Chien-Yuan Pan) | |
dc.subject.keyword | 矽奈米線場效電晶體,肝細胞癌,生物感測器, | zh_TW |
dc.subject.keyword | Hepatocellular carcinoma (HCC),silicon nanowire field-effect transistors (SiNW-FETs), | en |
dc.relation.page | 65 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-07-23 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 化學研究所 | zh_TW |
顯示於系所單位: | 化學系 |
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