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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 侯文祥(Wen-Shang Hou) | |
dc.contributor.author | Chan-Lao Chau | en |
dc.contributor.author | 周振鎏 | zh_TW |
dc.date.accessioned | 2021-06-16T13:05:16Z | - |
dc.date.available | 2013-08-07 | |
dc.date.copyright | 2013-08-07 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-02 | |
dc.identifier.citation | 李俊璋, 王應然, 孫逸民, 田倩蓉, 謝佳禕, 蔡逸婷, 彭國忠, 吳芬芬,
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Bacillus oceanisediminis sp. nov., isolated from marine sediment. International journal of systematic and evolutionary microbiology, 60(Pt 12), 2924-2929. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61537 | - |
dc.description.abstract | 甲醛 ( formaldehyde ) 是一種廣泛應用於醫療、農業、工業的化學物,因其可破壞蛋白質和核酸,故具有生物毒性。本研究於台灣北部沿岸 5 個地點採集海水樣本,進行甲醛耐受微生物的培養、計數和分離,並測試分離株是否具甲醛降解能力以及研究其降解效率。結果發現,各樣水中甲醛耐受微生物的菌數均大於 240 cells / ml。根據 16S rRNA gene 及 26S rRNA gene 的定序及比對,顯示 8 株可耐受 25 ppm 甲醛的分離株中,4 株為真菌,分別為 Galactomyces 屬(1 株)及 Candida 屬(3 株),另外 4 株為細菌,分別為 Enterobacter 屬(1 株)、 Pseudomonas 屬(1 株)及 Bacillus 屬(2 株)。所有菌株皆無法以甲醇作為單一碳源生長。除 2 株 Bacillus 屬細菌,其餘 6 株分離株具有較強的甲醛耐受能力,以之進行甲醛降解測試,當菌液起始濃度為 0.2 ( OD600 ) 時,可在 PY broth 中於 72 小時內分解 54 % 至 98 % 100 ppm 甲醛,而細菌分離株 B3 在 PY broth 中可於 48小時內分解 100 % 400 ppm 甲醛,於 72 小時內分解 59 % 700 ppm 甲醛,提升 B3 的菌液起始濃度成 0.55 至 0.6 ( OD600 ) 並以搖瓶方式培養,其於 24 小時內即可分解約 97 % 1500 ppm 或 60 % 3200 ppm 甲醛。綜合分子親緣分析及生理生化特性測試結果,推斷 B3 屬 Enterobacter cloacae。分離株 B3 於未來或可應用於處理甲醛廢水。 | zh_TW |
dc.description.abstract | Formaldehyde is a widely used chemical in medicine, agriculture and different industrial areas. It is biologically toxic because of its proteins and nucleic acid denaturing properties.
Coastal seawater samples were collected from 5 regions of northern Taiwan. Microorganisms were cultured, counted and isolated for studies of formaldehyde degradation ability and degradation efficiency. Eight formaldehyde-tolerant bacterial and fungal strains were isolated. All strains can tolerate up to 25 ppm formaldehyde, but cannot grow on methanol as a sole carbon source. The formaldehyde-tolerant microorganisms were found in all water samples with concentrations greater than 240 cells / ml. The fungal isolates were classified to the genus Galactomyces ( 1 strain ) or Candida ( 3 strains ).The bacterial isolates were classified to the genus Enterobacter ( 1 strain ), Pseudomonas ( 1 strain ) or Bacillus ( 2 strains ). Isolates ( except 2 strains of Bacillus ) were used for formaldehyde biodegrading study, 54 % ~ 98 % of 100 ppm formaldehyde were degraded by these strains over 72 hours when the culture’s initial optical density at 600 nm was 0.2. 100 % of 400 ppm or 59 % of 700 ppm formaldehyde in PY broth were degraded by strain B3 in 48 or 72 hours. Strain B3 could also degrade 97 % of 1500 ppm or 60 % 3200 ppm formaldehyde in PY broth over 24 hours when the culture’s initial optical density at 600 nm was 0.55 ~ 0.6, and incubated with shaking. 16S rRNA gene sequence and phenotype analysis indicated that strain B3 was Enterobacter cloacae. Strains B3 may be useful for treatment of waste water containing formaldehyde. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T13:05:16Z (GMT). No. of bitstreams: 1 ntu-102-R99622041-1.pdf: 4698958 bytes, checksum: ead0ab3616e9d02f3bc62dff7d206ec0 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iii 圖目錄 vi 表目錄 vii 附表目錄 viii 第一章 前言 1 第一節 甲醛及其毒性 1 第二節 甲醛於環境中的曝露及相關規範 1 第三節 甲基營養型微生物及其降解甲醛的生化途徑 2 第四節 甲醛降解微生物 3 第五節 甲醛降解微生物的應用 4 第六節 本研究的目標 5 第二章 材料及方法 6 第一節 培養基 6 第二節 樣本採集 6 第三節 微生物計數、分離與純化 7 第四節 甲醛降解測試 8 第五節 分離株形態及生理生化特性測試 9 第六節 分離株 rRNA 基因序列的取得並與基因資料庫比對 13 第七節 根據分離株 rRNA 基因序列的親緣分析 16 第三章 結果 19 第一節 甲醛耐受微生物生菌數計數 19 第二節 甲醛耐受微生物分離株的特性測試 19 第三節 各分離株的甲醛耐受及降解能力 19 第四節 各分離株 rRNA 基因序列之親緣分析及菌種鑑定 21 第四章 討論 25 第一節 分離株的分類地位探討 25 第二節 分離株耐受及降解甲醛的能力探討 26 第三節 甲醛對分離株生長的抑制作用 28 第四節 提升菌株的甲醛降解效率 28 總結 30 參考文獻 31 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣北部沿岸海水中甲醛降解微生物的分離和特性研究 | zh_TW |
dc.title | Isolation and characterization of formaldehyde-degrading microorganisms in coastal seawater of north Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 謝文陽(Wung-Yang Shieh) | |
dc.contributor.oralexamcommittee | 廖秀娟,劉玫英,陳卓昇 | |
dc.subject.keyword | 甲醛,生物降解,甲基營養型,腸桿菌屬,陰溝腸桿菌, | zh_TW |
dc.subject.keyword | Formaldehyde,biodegradation,methylotrophic,Enterobacter cloacae, | en |
dc.relation.page | 74 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-05 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物環境系統工程學研究所 | zh_TW |
顯示於系所單位: | 生物環境系統工程學系 |
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