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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61438完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 廖淑貞(Shwu-Jen Liaw) | |
| dc.contributor.author | Zong-Sian Li | en |
| dc.contributor.author | 李宗憲 | zh_TW |
| dc.date.accessioned | 2021-06-16T13:03:02Z | - |
| dc.date.available | 2018-09-24 | |
| dc.date.copyright | 2013-09-24 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-06 | |
| dc.identifier.citation | REFERENCES
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Jiang, S.S., et al., Characterization of UDP-glucose dehydrogenase and UDP-glucose pyrophosphorylase mutants of Proteus mirabilis: defectiveness in polymyxin B resistance, swarming, and virulence. Antimicrob Agents Chemother, 2010. 54(5): p. 2000-9. 59. Jansen, A.M., et al., Mannose-resistant Proteus-like fimbriae are produced by most Proteus mirabilis strains infecting the urinary tract, dictate the in vivo localization of bacteria, and contribute to biofilm formation. Infect Immun, 2004. 72(12): p. 7294-305. 60. Li, X., et al., Identification of MrpI as the sole recombinase that regulates the phase variation of MR/P fimbria, a bladder colonization factor of uropathogenic Proteus mirabilis. Mol Microbiol, 2002. 45(3): p. 865-74. 61. Clemmer, K.M. and P.N. Rather, Regulation of flhDC expression in Proteus mirabilis. Res Microbiol, 2007. 158(3): p. 295-302. 62. Pearson, M.M. and H.L. Mobley, The type III secretion system of Proteus mirabilis HI4320 does not contribute to virulence in the mouse model of ascending urinary tract infection. J Med Microbiol, 2007. 56(Pt 10): p. 1277-83. 63. Reading, N.C., et al., A transcriptome study of the QseEF two-component system and the QseG membrane protein in enterohaemorrhagic Escherichia coli O157 : H7. Microbiology, 2010. 156(Pt 4): p. 1167-75. 64. Moreira, C.G. and V. Sperandio, Interplay between the QseC and QseE bacterial adrenergic sensor kinases in Salmonella enterica serovar Typhimurium pathogenesis. Infect Immun, 2012. 80(12): p. 4344-53. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61438 | - |
| dc.description.abstract | Proteus mirabilis is a facultative anaerobic, Gram-negative bacterium and a member of the Enterobacteriaceae family. It belongs to normal flora of human intestine. It usually causes, however, urinary tract infection (UTI), and leads to kidney disease, pneumonia and septicemia in individuals with long-term catheterization or with structural or functional abnormalities in the urinary tract.
QseEGF, first described in enterohaemorrhagic Escherichia coli, is one of two-component systems in bacteria. QseEGF can sense signals like bacterial AI-2 and epinephrine from host cells. Activation of QseEGF regulates virulence genes, like LEE genes, causing attaching and effacing lesions (A/E lesions), or Shiga toxin, causing hemolytic-uremic syndrome (HUS). To understand the role of QseEGF in P. mirabilis, in this study we constructed qseEGF mutant. We found mutation in qseEGF caused pleiotropic phenotypic effects in P. mirabilis. The swarming ability of qseEGF mutant was lower than wild-type (Wt). In this regard, production of swarmer cells of the qseEGF mutant was delayed and swarm cell length was much shorter than Wt. The swarming ability was restored to near the wt level in the QseEGF-complemented strain. Besides, we demonstrated the qseEGF mutant displayed decreased haemolysin ability and cytotoxicity. We next explore why QseEGF regulates swarming motility. We found mutation in qseEGF decreased flhDC and flagella expression in swarming condition. In addition, we tested possible signals of QseEGF. We found urea inhibited the swarming motility of Wt but not in qseEGF mutant. Reporter assay also showed flhDC expression of Wt but not qseEGF mutant was decreased in the presence of urea. We thought that urea was one negative signal for the QseEGF signaling pathway. To further understand the role of qseEGF gene in swarming, we tested rcsB, which negatively regulates flhDC expression, expression in Wt and qseEGF mutant. We found expression rcsB in qseEGF mutant was higher than Wt. Knowing MR/P frimbriae are required for adhering to urinary tract epithelial cells of mouse, we examined the role of QseEGF in the expression of MR/P fimbriae and found that qseEGF mutant didn’t produce MrpA protein, the subunit of MR/P frimbriae, by western blot. In summary, we found that QseEGF plays an important role in swarming of P. mirabilis by sensing the surface contact stimulus or urea to positively or negatively regulate flhDC expression. Besides, QseEGF also regulates expression of virulence factors such as MR/P fimbriae and hence might contribute to UTI of P. mirabilis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T13:03:02Z (GMT). No. of bitstreams: 1 ntu-102-R00424024-1.pdf: 9412168 bytes, checksum: 0e19936a781809292e4b5cf438f27c40 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 # 誌謝 i 中文摘要 ii ABSTRACT iv 目錄 vi 表目錄 viii 圖目錄 ix 第一章 緒論 1 第一節 奇異變形桿菌(Proteus mirabilis)介紹 1 第二節 Quorum sensing E. coli regulator EGF (qseEGF)的基本介紹 7 第三節 研究動機與目的 8 第四節 實驗設計 9 第二章 實驗材料與方法 10 第一節 實驗材料 10 第二節 qseEGF(PMI1874，PMI1873，PMI1872) knockout方法 12 第三節 分析突變株表現型(phenotype)及毒力因子 (virulence factor) 表現 23 第四節 qseEGF參與之基因調控分析 35 第三章 實驗結果 50 第一節 qseEGF Knockout strain 建立 50 第二節 qseEGF knockout 突變株之表現型之分析 51 第三節 qseEGF knockout 突變株毒力因子分析 52 第四節 分析qseEGF 可能調控的路徑 53 第四章 結論與討論 56 第一節 結論 56 第二節 其他菌種QseEGF 之研究比較 57 第三節 在文章中引用到Equation Number 58 第四節 未來展望 59 Chapter 5 表 60 Chapter 6 圖 64 Chapter 7 附錄 85 REFERENCE 102 | |
| dc.language.iso | zh-TW | |
| dc.subject | 奇異變形桿菌 | zh_TW |
| dc.subject | 雙組成系統 | zh_TW |
| dc.subject | 尿素 | zh_TW |
| dc.subject | 毒力 | zh_TW |
| dc.subject | 表面移行 | zh_TW |
| dc.subject | qseEGF | en |
| dc.subject | Two-compenent system(TCS) | en |
| dc.subject | swarming | en |
| dc.subject | virulence | en |
| dc.subject | urea | en |
| dc.title | 奇異變形桿菌qseEGF之探討 | zh_TW |
| dc.title | Characterization of Proteus mirabilis qseEGF | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄧麗珍(Lee-Jene Teng),賴信志(Hsin-Chih Lai),楊翠青(Tsuey-Ching Yang) | |
| dc.subject.keyword | 雙組成系統,奇異變形桿菌,表面移行,毒力,尿素, | zh_TW |
| dc.subject.keyword | qseEGF,Two-compenent system(TCS),swarming,virulence,urea, | en |
| dc.relation.page | 105 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-06 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
| 顯示於系所單位： | 醫學檢驗暨生物技術學系 | |
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