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標題: | 阿拉伯芥FIN219與FHY1的交互作用對遠紅光和茉莉酸訊息傳遞的影響 The effects of FIN219 and FHY1 interaction on far-red light and jasmonate signal integration in Arabidopsis thalianasis |
作者: | Chien-Wei Lai 賴建瑋 |
指導教授: | 謝旭亮 |
關鍵字: | 遠紅光,茉莉酸, FHY1, |
出版年 : | 2013 |
學位: | 碩士 |
摘要: | 植物可隨時偵測周圍環境,並相應地改變自己的生理代謝,生長和發育。對於控制植物的光形態發生,光和各種激素之間的交互作用是不可缺少的。在幼苗時期時FIN219參與 phyA所調控的遠紅光訊息傳遞路徑,同時和JAR1位於同一基因座上,是參與茉莉酸訊息傳遞的重要因子。FHY1基因則是遠紅光訊息傳遞下游的正向調控者,同時也扮演著將PHYA帶入細胞核中的角色。前人研究指出FHY1和FIN219在遠紅光下具有某些程度的交互作用。然而對於其詳細分子機制及遺傳背景的作用依然有很多不清楚的地方。這裡我們發現在 FIN219大量表現在 fhy1-1 或FHY1大量表現在fin219-2時皆可部分rescue其突變株較為抽長的下胚軸。利用pull-down和共免疫沉澱法 (Co-Immunoprecipitation) 證明兩者在光照下才會產生交互作用,並證實FIN219是以C端和FHY1進行交互作用。另外利用西方點墨法及反轉錄PCR (reverse transcription-PCR )檢測,發現FIN219和FHY1會互相抑制對方的表現量。此外,在甲基茉莉酸 (MeJA)處理的實驗中,也顯示兩者參與茉莉酸所調控的一些生理反應,包括花青素的累積。觀察PHYA-GFP於細胞內的位置,可發現在FIN219大量表現時, PHYA不易進入細胞核,可能由於FIN219會和PHYA競爭FHY1,導致PHYA進核的比例下降。因此,本論文的研究結果顯示FIN219與FHY1彼此交互作用,造成互相拮抗的效果,以調控阿拉芥PHYA所引發的光型態發生,以及茉莉酸訊息傳遞。 Plants continuously monitor their surrounding environments and change accordingly their physiological metabolism, growth and development. The cross-talks between light and various hormones are essential for controlling plant photomorphogenesis. During the seedling stage, FAR-RED INSENSITIVE 219 (FIN219) was involved in phytochrome A-mediated far-red (FR) light signaling and JA responses in Arabidopsis. Far-red Elongated Hypocotyl l (FHY1), a positive regulator of FR signaling, is required for FR-regulated nuclear accumulation of phyA. Previous study showed that FIN219 and FHY1 interacted genetically under FR light. However, the molecular mechanisms underlying the genetic interaction between FIN219 and FHY1 remain largely unknown. Here, we found that over-expression of either FIN219 in fhy1-1 or FHY1 in fin219-2 mutants can partially rescue their respective long-hypocotyl phenotypes. Using pull-down and co-immunoprecipitation (Co-IP) assays demonstrated that FIN219 and FHY1 interacted with each other. Moreover, the C-terminal region of FIN219 is responsible for the interaction with FHY1. Besides, FIN219 and FHY1 was antagonistic to each other at their transcript and protein levels. Further MeJA-treated experiments revealed that FIN219 and FHY1 were involved in the regulation of MeJA-mediated responses, including anthocyanin accumulation. In subcellular localization studies, FIN219 and PHYA may compete for FHY1, leading to decreased nuclear import of PHYA.Taken together, these data indicate that FIN219 and FHY1 interact with each other, and their mutual repression regulates phyA-mediated photomorphogenesis and JA signaling pathway in Arabidopsis. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6121 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 植物科學研究所 |
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