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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡偉博(Wei-Bor Tsai) | |
dc.contributor.author | Chih-Yuan Chien | en |
dc.contributor.author | 簡志遠 | zh_TW |
dc.date.accessioned | 2021-06-16T10:49:49Z | - |
dc.date.available | 2018-08-16 | |
dc.date.copyright | 2013-08-16 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-12 | |
dc.identifier.citation | [1] Silverman HG, Roberto FF. Understanding marine mussel adhesion. Mar Biotechnol (NY). 2007;9:661-81.
[2] Hamming LM, Fan XW, Messersmith PB, Brinson LC. Mimicking mussel adhesion to improve interfacial properties in composites. Compos Sci Technol. 2008;68:2042-8. [3] Lee H, Dellatore SM, Miller WM, Messersmith PB. Mussel-inspired surface chemistry for multifunctional coatings. Science. 2007;318:426-30. [4] Yu M, Hwang J, Deming TJ. Role of l-3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins. Journal of the American Chemical Society. 1999;121:5825-6. [5] Lee H, Scherer NF, Messersmith PB. Single-molecule mechanics of mussel adhesion. Proc Natl Acad Sci U S A. 2006;103:12999-3003. [6] Burzio LA, Waite JH. Cross-linking in adhesive quinoproteins: studies with model decapeptides. Biochemistry. 2000;39:11147-53. [7] Yamamoto H. Adhesive studies of synthetic polypeptides: A model for marine adhesive proteins. J Adhes Sci Technol. 1987;1. [8] Unsworth LD, Sheardown H, Brash JL. Protein resistance of surfaces prepared by sorption of end-thiolated poly(ethylene glycol) to gold: effect of surface chain density. Langmuir. 2005;21:1036-41. [9] Lee H, Lee KD, Pyo KB, Park SY. Catechol-grafted poly(ethylene glycol) for PEGylation on versatile substrates. Langmuir. 2010;26:3790-3. [10] Li G, Cheng G, Xue H, Chen S, Zhang F, Jiang S. Ultra low fouling zwitterionic polymers with a biomimetic adhesive group. Biomaterials. 2008;29:4592-7. [11] Gao C, Li G, Xue H, Yang W, Zhang F, Jiang S. Functionalizable and ultra-low fouling zwitterionic surfaces via adhesive mussel mimetic linkages. Biomaterials. 2010;31:1486-92. [12] Waite JH. Surface chemistry: Mussel power. Nat Mater. 2008;7:8-9. [13] Ku SH, Park CB. Human endothelial cell growth on mussel-inspired nanofiber scaffold for vascular tissue engineering. Biomaterials. 2010;31:9431-7. [14] Ku SH, Ryu J, Hong SK, Lee H, Park CB. General functionalization route for cell adhesion on non-wetting surfaces. Biomaterials. 2010;31:2535-41. [15] Ratner B. The blood compatibility catastrophe. J Biomed Mater Res. 1993;27:283-7. [16] Higuchi A, et al. Bioinert surface of pluronic-immobilized flask for preservation of hematopoietic stem cells. Biomacromolecules. 2006;7:1083-9. [17] Mendelsohn JD, Yang SY, Hiller J, Hochbaum AI, Rubner MF. Rational design of cytophilic and cytophobic polyelectrolyte multilayer thin films. Biomacromolecules. 2003;4:96-106. [18] Jeon SI, Lee JH, Andrade JD, De Gennes PG. Protein--surface interactions in the presence of polyethylene oxide: I. Simplified theory. Journal of Colloid and Interface Science. 1991;142:149-58. [19] Hoffman AS. Non-fouling surface technologies. J Biomater Sci Polym Ed. 1999;10:1011-4. [20] Elbert DL, Hubbell JA. Self-assembly and steric stabilization at heterogeneous, biological surfaces using adsorbing block copolymers. Chem Biol. 1998;5:177-83. [21] Huang NP, et al. Poly(l-lysine)-g-poly(ethylene glycol) layers on metal oxide surfaces: Surface-analytical characterization and resistance to serum and fibrinogen adsorption. Langmuir. 2000;17:489-98. [22] Brady MA, Limpoco FT, Perry SS. Solvent-dependent friction force response of poly(ethylenimine)-graft-poly(ethylene glycol) brushes investigated by atomic force microscopy. Langmuir. 2009;25:7443-9. [23] Amirpour ML, Ghosh P, Lackowski WM, Crooks RM, Pishko MV. Mammalian cell cultures on micropatterned surfaces of weak-acid, polyelectrolyte hyperbranched thin films on gold. Anal Chem. 2001;73:1560-6. [24] Archambault JG, Brash JL. Protein resistant polyurethane surfaces by chemical grafting of PEO: amino-terminated PEO as grafting reagent. Colloids Surf, B. 2004;39:9-16. [25] Lee JH, Jeong BJ, Lee HB. Plasma protein adsorption and platelet adhesion onto comb-like PEO gradient surfaces. J Biomed Mater Res. 1997;34:105-14. [26] Thissen H, Gengenbach T, du Toit R, Sweeney DF, Kingshott P, Griesser HJ, Meagher L. Clinical observations of biofouling on PEO coated silicone hydrogel contact lenses. Biomaterials. 2010;31:5510-9. [27] Cancedda R, Dozin B, Giannoni P, Quarto R. Tissue engineering and cell therapy of cartilage and bone. Matrix Biol. 2003;22:81-91. [28] Salgado AJ, Coutinho OP, Reis RL. Bone tissue engineering: state of the art and future trends. Macromol Biosci. 2004;4:743-65. [29] Paital SR, Dahotre NB. Calcium phosphate coatings for bio-implant applications: Materials, performance factors, and methodologies. Materials Science and Engineering: R: Reports. 2009;66:1-70. [30] Ozeki K, Yuhta T, Fukui Y, Aoki H, Nishimura I. A functionally graded titanium/hydroxyapatite film obtained by sputtering. Journal of Materials Science: Materials in Medicine. 2002;13:253-8. [31] Williams DF. Titanium and titanium alloys. In: Williams DF, editor. Biocompatibility of clinical implant materials. Florida: CRC Press Inc.; 1981. p. 199-222. [32] Thamaraiselvi T, Rajeswari S. Biological evaluation of bioceramic materials - a review. Trends in Biomaterials and Artificial Organs. 2004;18:9-17. [33] Wang G, Zreiqat H. Functional Coatings or Films for Hard-Tissue Applications. Materials. 2010;3:3994-4050. [34] Li SH, De Wijn JR, Layrolle P, De Groot K. Synthesis of macroporous hydroxyapatite scaffolds for bone tissue engineering. Journal of Biomedical Materials Research. 2002;61:109-20. [35] Chapman MW, Bucholz R, Cornell C. Treatment of acute fractures with a collagen-calcium phosphate graft material. A randomized clinical trial. J Bone Joint Surg Am. 1997;79:495-502. [36] Le Guehennec L, Soueidan A, Layrolle P, Amouriq Y. Surface treatments of titanium dental implants for rapid osseointegration. Dental Materials. 2007;23:844-54. [37] Tonino AJ, Rahmy AIA. The hydroxyapatite-ABG hip system: 5- to 7-Year results from an international multicentre study. The Journal of Arthroplasty. 2000;15:274-82. [38] De Groot K, Geesink R, Klein CPAT, Serekian P. Plasma sprayed coatings of hydroxylapatite. Journal of Biomedical Materials Research. 1987;21:1375-81. [39] Zeng H, Lacefield WR. The study of surface transformation of pulsed laser deposited hydroxyapatite coatings. J Biomed Mater Res. 2000;50:239-47. [40] Zhitomirsky I, Gal-Or L. Electrophoretic deposition of hydroxyapatite. J Mater Sci Mater Med. 1997;8:213-9. [41] Robert B H. Thermal spraying of biomaterials. Surface and Coatings Technology. 2006;201:2012-9. [42] Xue W, Tao S, Liu X, Zheng X, Ding C. In vivo evaluation of plasma sprayed hydroxyapatite coatings having different crystallinity. Biomaterials. 2004;25:415-21. [43] Ruoslahti E, Pierschbacher M. New perspectives in cell adhesion: RGD and integrins. Science. 1987;238:491-7. [44] Rezania A, Thomas CH, Branger AB, Waters CM, Healy KE. The detachment strength and morphology of bone cells contacting materials modified with a peptide sequence found within bone sialoprotein. J Biomed Mater Res. 1997;37:9-19. [45] Barber TA, Gamble LJ, Castner DG, Healy KE. In vitro characterization of peptide-modified p(AAm-co-EG/AAc) IPN-coated titanium implants. J Orthop Res. 2006;24:1366-76. [46] Kardestuncer T, McCarthy MB, Karageorgiou V, Kaplan D, Gronowicz G. RGD-tethered silk substrate stimulates the differentiation of human tendon cells. Clin Orthop Relat Res. 2006;448:234-9. [47] Schaffner P, Dard MM. Structure and function of RGD peptides involved in bone biology. Cell Mol Life Sci. 2003;60:119-32. [48] Hogan BL. Bone morphogenetic proteins: multifunctional regulators of vertebrate development. Genes Dev. 1996;10:1580-94. [49] Rengachary SS. Bone morphogenetic proteins: basic concepts. Neurosurg Focus. 2002;13:e2. [50] Urist MR. Bone morphogenetic protein: the molecularization of skeletal system development. J Bone Miner Res. 1997;12:343-6. [51] Chen G, Deng C, Li YP. TGF-beta and BMP signaling in osteoblast differentiation and bone formation. Int J Biol Sci. 2012;8:272-88. [52] Cheng H, et al. Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am. 2003;85-A:1544-52. [53] von Bubnoff A, Cho KW. Intracellular BMP signaling regulation in vertebrates: pathway or network? Dev Biol. 2001;239:1-14. [54] Yamaguchi A, Komori T, Suda T. Regulation of osteoblast differentiation mediated by bone morphogenetic proteins, hedgehogs, and Cbfa1. Endocr Rev. 2000;21:393-411. [55] Noshi T, et al. Recombinant human bone morphogenetic protein-2 potentiates the in vivo osteogenic ability of marrow/hydroxyapatite composites. Artif Organs. 2001;25:201-8. [56] Reddi AH. Morphogenetic messages are in the extracellular matrix: biotechnology from bench to bedside. Biochem Soc Trans. 2000;28:345-9. [57] Alam MI, Asahina I, Ohmamiuda K, Takahashi K, Yokota S, Enomoto S. Evaluation of ceramics composed of different hydroxyapatite to tricalcium phosphate ratios as carriers for rhBMP-2. Biomaterials. 2001;22:1643-51. [58] Li RH, Wozney JM. Delivering on the promise of bone morphogenetic proteins. Trends Biotechnol. 2001;19:255-65. [59] Tsai WB, Chen WT, Chien HW, Kuo WH, Wang MJ. Poly(dopamine) coating of scaffolds for articular cartilage tissue engineering. Acta Biomaterialia. 2011;7:4187-94. [60] Wei Q, et al. Improving the blood compatibility of material surfaces via biomolecule-immobilized mussel-inspired coatings. J Biomed Mater Res A. 2011;96:38-45. [61] Tsai WB, Chien CY, Thissen H, Lai JY. Dopamine-assisted immobilization of poly(ethylene imine) based polymers for control of cell-surface interactions. Acta Biomater. 2011;7:2518-25. [62] Tsai WB, Shi Q, Grunkemeier JM, McFarland C, Horbett TA. Platelet adhesion to radiofrequency glow-discharge-deposited fluorocarbon polymers preadsorbed with selectively depleted plasmas show the primary role of fibrinogen. J Biomater Sci Polym Ed. 2004;15:817-40. [63] Tsai WB, Chen YH, Chien HW. Collaborative cell-resistant properties of polyelectrolyte multilayer films and surface PEGylation on reducing cell adhesion to cytophilic surfaces. J Biomater Sci Polym Ed. 2009;20:1611-28. [64] Kuo WH, Wang MJ, Chien HW, Wei TC, Lee C, Tsai WB. Surface Modification with Poly(sulfobetaine methacrylate-co-acrylic acid) To Reduce Fibrinogen Adsorption, Platelet Adhesion, and Plasma Coagulation. Biomacromolecules. 2011;12:4348-56. [65] Tsai WB, Chen RP, Wei KL, Chen YR, Liao TY, Liu HL, Lai JY. Polyelectrolyte multilayer films functionalized with peptides for promoting osteoblast functions. Acta Biomater. 2009;5:3467-77. [66] Helfrish MH, H.Ralston S. Bone research protocols. Totowa, New Jersey: Humana Press. [67] Lee H, Rho J, Messersmith PB. Facile Conjugation of Biomolecules onto Surfaces via Mussel Adhesive Protein Inspired Coatings. Adv Mater Deerfield. 2009;21:431-4. [68] Tsai WB, Chen RP, Wei KL, Tan SF, Lai JY. Modulation of RGD-functionalized polyelectrolyte multilayer membranes for promoting osteoblast function. J Biomater Sci Polym Ed. 2010;21:377-94. [69] Chua PH, Neoh KG, Kang ET, Wang W. Surface functionalization of titanium with hyaluronic acid/chitosan polyelectrolyte multilayers and RGD for promoting osteoblast functions and inhibiting bacterial adhesion. Biomaterials. 2008;29:1412-21. [70] LaVoie MJ, Ostaszewski BL, Weihofen A, Schlossmacher MG, Selkoe DJ. Dopamine covalently modifies and functionally inactivates parkin. Nat Med. 2005;11:1214-21. [71] Kingshott P, Thissen H, Griesser HJ. Effects of cloud-point grafting, chain length, and density of PEG layers on competitive adsorption of ocular proteins. Biomaterials. 2002;23:2043-56. [72] Wilchek M, Bayer EA. Introduction to avidin-biotin technology. Methods Enzymol. 1990;184:5-13. [73] Bhat VD, Truskey GA, Reichert WM. Fibronectin and avidin-biotin as a heterogeneous ligand system for enhanced endothelial cell adhesion. J Biomed Mater Res. 1998;41:377-85. [74] Tsai WB, Wang MC. Effects of an avidin-biotin binding system on chondrocyte adhesion and growth on biodegradable polymers. Macromol Biosci. 2005;5:214-21. [75] Tsai WB, Wang MC. Effect of an avidin-biotin binding system on chondrocyte adhesion, growth and gene expression. Biomaterials. 2005;26:3141-51. [76] Wilchek M BE. Introduction to avidin-biotin technology. San Diego: Academic Press Inc.; 1990. [77] Metzger SW NM, Yanavich C, Schneider J, Lee GU. Development and characterization of surface chemistries for microfabricated biosensors. J Vac Sci Technol A. 1999;17:2623-8. [78] Huang N. P. VJ, De Paul S. M., Textor M. and Spencer N. D. Biotin-derivatized poly(l-lysine)-g-poly(ethylene glycol): a novel polymeric interface for bioaffinity sensing. Langmuir. 2002;18:220-30. [79] Gunawan RC, King JA, Lee BP, Messersmith PB, Miller WM. Surface presentation of bioactive ligands in a nonadhesive background using DOPA-tethered biotinylated poly(ethylene glycol). Langmuir. 2007;23:10635-43. [80] Liu X, Chu PK, Ding C. Surface modification of titanium, titanium alloys, and related materials for biomedical applications. Materials Science and Engineering: R: Reports. 2004;47:49-121. [81] Rodrı R, Blesa MA, Regazzoni AE. Surface Complexation at the TiO2(anatase)/Aqueous Solution Interface: Chemisorption of Catechol. Journal of Colloid and Interface Science. 1996;177:122-31. [82] Dalsin JL, Lin L, Tosatti S, Voros J, Textor M, Messersmith PB. Protein resistance of titanium oxide surfaces modified by biologically inspired mPEG-DOPA. Langmuir. 2005;21:640-6. [83] Wach JY, Malisova B, Bonazzi S, Tosatti S, Textor M, Zurcher S, Gademann K. Protein-resistant surfaces through mild dopamine surface functionalization. Chemistry. 2008;14:10579-84. [84] Lock J, Liu H. Nanomaterials enhance osteogenic differentiation of human mesenchymal stem cells similar to a short peptide of BMP-7. Int J Nanomedicine. 2011;6:2769-77. [85] Facer SR, Zaharias RS, Andracki ME, Lafoon J, Hunter SK, Schneider GB. Rotary culture enhances pre-osteoblast aggregation and mineralization. J Dent Res. 2005;84:542-7. [86] Stains JP, Civitelli R. Cell-cell interactions in regulating osteogenesis and osteoblast function. Birth Defects Res C Embryo Today. 2005;75:72-80. [87] Tsai WB, Chen YR, Liu HL, Lai JY. Fabrication of UV-crosslinked chitosan scaffolds with conjugation of RGD peptides for bone tissue engineering. Carbohydrate Polymers. 2011;85:129-37. [88] Elmengaard B, Bechtold JE, Soballe K. In vivo study of the effect of RGD treatment on bone ongrowth on press-fit titanium alloy implants. Biomaterials. 2005;26:3521-6. [89] Elmengaard B, Bechtold JE, Soballe K. In vivo effects of RGD-coated titanium implants inserted in two bone-gap models. Journal of biomedical materials research Part A. 2005;75:249-55. [90] Ferris DM, Moodie GD, Dimond PM, Giorani CWD, Ehrlich MG, Valentini RF. RGD-coated titanium implants stimulate increased bone formation in vivo. Biomaterials. 1999;20:2323-31. [91] Itoh D, et al. Enhancement of osteogenesis on hydroxyapatite surface coated with synthetic peptide (EEEEEEEPRGDT) in vitro. J Biomed Mater Res. 2002;62:292-8. [92] Gilbert M, Giachelli CM, Stayton PS. Biomimetic peptides that engage specific integrin-dependent signaling pathways and bind to calcium phosphate surfaces. J Biomed Mater Res A. 2003;67:69-77. [93] Takeuchi Y, Suzawa M, Kikuchi T, Nishida E, Fujita T, Matsumoto T. Differentiation and transforming growth factor-beta receptor down-regulation by collagen-alpha2beta1 integrin interaction is mediated by focal adhesion kinase and its downstream signals in murine osteoblastic cells. J Biol Chem. 1997;272:29309-16. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61155 | - |
dc.description.abstract | 抗污表面 (anti-fouling surface) 在生醫器材中扮演重要的角色。在第一部分 實驗中,透過多巴胺 (dopamine) 聚合,我們發展出一種製作抗污表面,以及使 表面帶有生物素 (biotin) 用於生醫器材。聚乙烯亞胺接枝聚乙二醇( poly(ethylene - imine)-graft-poly(ethylene glycol), PEI-g-PEG ) 混合多巴胺鹼性溶液後,將欲處 理表面沉浸至該溶液兩小時,即製作出可以抵抗血清蛋白以及纖維母細胞 (fibroblast) 的吸附 (adsorption) 及貼附 (adhesion)。此外,透過多巴胺鹼性環境 聚合反應共沉積聚乙烯亞胺,接枝聚乙二醇和聚乙烯亞胺接枝生物素,可製作出 一以抗污表面做為背景,然而同時又可提供可反應之生物素分子。我們展示此表 面可透過卵白素-生物素 (avidin-biotin) 結合系統產生選擇性結合,之後再結合 精胺酸-甘胺酸-天冬胺酸-絲胺酸-生物素 (Arg-Gly- Asp-Ser-biotin, RGDS-biotin) 透過細胞-配合基 (cell-ligand) 交互作用而增加細胞貼附。
改植氫氧基磷灰石 (hydroxyapatite) 於骨科移植物上為一常見增加骨引導 (osteocondution) 之方法。在第二部分實驗中,透過多巴胺聚合反應我們發展出 一種簡易氫氧基磷灰石表面改質方法,以用於骨移植物上。氫氧基磷灰石奈米 粒子混入多巴胺鹼性溶液後,將移植物(鈦金屬)沉浸於該溶液中,即可於植入 物表面形成一層氫氧基磷灰石/多巴胺之膜。此氫氧基磷灰石/多巴胺之沉積膜可 增加骨母細胞 (osteoblast) 貼附、增生和骨礦化表現。此外,固定擁有精胺酸- 甘胺酸-天冬胺酸之胜肽序列於已將氫氧基磷灰石/多巴胺改植之表面,可促進 骨母細胞貼附及分化。 骨型態發生蛋白 (bone morphogenetic proteins, BMPs) 已被證實於骨科治療 下擁有骨誘導 (osteoinduction) 之功能。在第三部分實驗中,透過多巴胺聚合反 應我們發展出一種簡單將第二型骨型態發生蛋白和氫氧基磷灰石和精胺酸-甘胺 酸-天冬胺酸之胜肽序列同時改植於骨植物入表面之方法。將骨植入物(鈦金屬) 浸泡於此三生物分子之多巴胺鹼性溶液中,使此表面同時具有骨誘導、骨引導以 及增加細胞貼附之功能表面。此表面增加間葉幹細胞 (mesenchymal stem cell) 之 細胞貼附以及骨分化。此外,透過多巴胺沉積第二型骨型態發生蛋白 (BMP-2) 使間葉幹細胞分化成骨母細胞於無骨分化培養基下以及不使蛋白質變性。 | zh_TW |
dc.description.abstract | Non-fouling coatings play a critical role in many biomedical applications. In the first part of this work, we have developed a facile one-step deposition method for preparation of non-fouling surfaces and biotinylated surfaces for biomedical applications. Poly(ethylene-imine)-graft-poly(ethylene glycol) copolymer (PEI-g-PEG) was mixed with an alkaline dopamine solution and then deposited onto substrates as a coating for 2h, which inhibited the adsorption of serum proteins and the attachment of fibroblasts. Furthermore, co-deposition of PEI-g-PEG and PEI-g-biotin in alkaline dopamine solutions provided a cell-resisting background surface while at the same time providing accessible biotin molecules. We demonstrated that this surface can be used for the selective binding of avidin, and the subsequent binding of RGDS-biotin enhanced cell attachment by specific cell-ligand interactions.
Hydroxyapatite (HAp) coating on orthopaedic implants is a common strategy to increase osteointegration. In the second of this work, a facile deposition method based on dopamine polymerization was developed for preparation of HAp-coated titanium substrates for orthopaedic applications. Nano-structured HAp was mixed with an alkaline dopamine solution and then deposited onto titanium to form a dopamine/HAp ad-layer. The deposition of dopamine/HAp greatly enhanced the adhesion, proliferation and mineralization of osteoblasts. Furthermore, RGD-containing peptides were immobilized to dopamine/HAP coated titanium and further enhanced cell adhesion and osteogenic differentiation. Bone morphogenetic proteins (BMPs) have been proved to improve osteoinduction on orthopedic therapies. In the third part of this work, a facile deposition method based on dopamine polymerization was developed for preparation of RGD/HAp/BMP-2-coated substrates for orthopaedic applications. HAp, RGD and BMP-2 was mixed with an alkaline dopamine solution and then deposited onto titanium to form a dopamine/RGD/HAp/BMP-2 ad-layer. This layer improved mesenchymal stem cell (MSC) line adhesion and osteodifferentiation. Furthermore, the immobilization of BMP-2 via dopamine polymerization promote MSC differentiate into osteoblast without osteogenic supplement and occuration of protein denaturation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T10:49:49Z (GMT). No. of bitstreams: 1 ntu-102-R99524021-1.pdf: 5373453 bytes, checksum: fd5cdd2fc90e2814642e215fc40ac547 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 摘要 I
Abstract III Content V Figure XI Table XVIII Chapter 1 1 1.1 Surface modification for Biomaterials 1 1.2 Mussel adhesive proteins 2 1.3 Anti-fouling surface 8 1.4 Poly(ethylene glycol) (PEG) surface modification 9 1.5 Bone implants 13 1.6 Calcium phosphate-base bioceramics 15 1.5 Common strategies for coating Ca-P bioceramics 17 1.5-1 Thermal plasma spraying 17 1.5-2 Pulsed laser deposition 19 1.5-3 Electrophoretic deposition 20 1.6 Effects of Arg-Gly-Asp (RGD) pepetides to cells 21 1.7 Bone morphogenetic proteins (BMPs) 24 1.8 Research motivation 27 1.9 Research Specific aim 30 Chapter 2 33 2.1 Chemicals 33 2.1-1 Synthesis of PEI-g-PEG and PEI-g-biotin 33 2.1-2 Dopamine-assisted deposition of PEI-g-PEG and PEI-g-biotin onto various polymeric substrates 34 2.1-3 Avidin adsorption 34 2.1-4 Cell number determination (Lactate dehydrogenase, LDH assay) 35 2.1-5 Mouse fibroblast-like cell line L929 culture 35 2.1-6 Osteoblast-like cell line MG63 culture 36 2.1-7 Primary osteoblast isolation and culture 37 2.1-8 Mineralization culture 38 2.1-9 Synthesis of hydroxyapatite 38 2.1-10 Dopamine-assisted deposition of HAp nanoparticles onto titanium substrates 38 2.1-11 Alkaline phosphatase (ALP) activity 39 2.1-12 Alizarin red staining 39 2.1-13 Quantification of calcium deposition 39 2.1-14 Reverse transcription-polymerase chain reaction (RT-PCR) 39 2.1-15 Qualitative test of immobilization of BMP-2 40 2.1-16 hMSC cell line (3A6) cell culture and osteogenic differentiation 41 2.2 Experimental instrument and consumable materials 42 2.2-1 Experimental instrument 42 2.2-2 Experimental consumable materials 42 2.3 Solution formula 43 2.3-1 Phophate buffered saline solution (PBS), pH 7.4 43 2.3-2 Alpha-MEM culture medium 43 2.3-4 LDH assay working solution 44 2.3-5 Tris-base buffer pH 8.5 44 2.3-6 DMEM low glucose medium for 3A6 cell culture 44 2.4 Methods 45 2.4-1 Synthesis of PEI-g-PEG and PEI-g-biotin 45 2.4-2 Dopamine-assisted deposition of PEI-g-PEG and PEI-g-biotin onto various polymeric substrates 47 2.4-3 XPS Analysis 49 2.4-4 Cell experiments for L929 50 2.4-5 Lactate dehydrogenase (LDH) assay 50 2.4-6 QCM measurements for PEI-g-PEG/dopamine deposition and serum protein adsorption 52 2.4-7 Avidin adsorption 53 2.4-8 Synthesis of hydroxyapatite 54 2.4-9 Dopamine-assisted deposition of HAp nanoparticles onto titanium substrates 55 2.4-10 Surface characterization of HAp/dopamine coated titanium 56 2.4-11 Osteoblast culture 56 2.4-12 Alizarin red staining 59 2.4-13 Alkaline phosphatase (ALP) activity 59 2.4-14 Calcium quantification 60 2.4-15 Reverse transcription-polymerase chain reaction (RT-PCR) 60 2.4-16 Qualitative test of immobilization of BMP-2 66 2.4-17 Statistical analysis 67 Chapter 3 68 3.1 XPS Analysis of TCPS deposited with PEI-g-PEG/dopamine 68 3.2 Measurement and evaluation of cell-repellent property of PEI-g-PEG/dopamine surfaces 69 3.3 The effects of pH of dopamine solutions on protein adsorption and cell adhesion 72 3.4 Surface biotinylation via dopamine polymerization 74 3.5 Discussion 74 3.5 Conclusion 80 Chapter 4 95 4.1 Culture of MG63 cells on dopamine/HAp deposited PS 95 4.2 Surface characterization of dopamine-deposited titanium 97 4.3 Culture of primary rat osteoblasts on dopamine/HAp coated titanium 98 4.4 The effect of RGD conjugation on culture of MG63 cells on dopamine/HAp coated titanium 99 4.5 Discussion 101 4.6 Conclusion 105 Chapter 5 118 6.1 Surface characterization of dopamine-deposited titanium 118 6.2 Culture of 3A6 cells on dopamine/PEI-g-RGD deposited titanium 119 6.3 Culture of 3A6 cells on dopamine/PEI-g-RGD/HAp deposited titanium 121 6.4 Culture of 3A6 cells on dopamine/PEI-g-RGD/HAp/BMP-2 deposited titanium 122 6.5 Discussion 126 6.6 Conclusion 132 Chapter 6 151 Chapter 7 158 7-1. Gene Primer 158 7-1.1 GAPDH 158 7-1.2 RunX2 159 7-1.2 Alkaline phosphatase (ALP) 160 7-1.3 Osteopontin (OPN) 163 7-1.4 Bone sialoprotein (BSP) 164 7-1.5 Osteocalcin (OCN) 166 7-2. X-ray photoelectron spectroscopy (XPS) 166 | |
dc.language.iso | en | |
dc.title | 多巴胺輔助固定生物分子應用於抗污表面及骨植入物 | zh_TW |
dc.title | Dopamine-assisted immobilization of biomolecules for applications of anti-fouling surface and bone implant | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡曉雯(Shiao-Wen Tsai),王孟菊(Meng-Jiy Wang),游佳欣(Jia-Shing Yu) | |
dc.subject.keyword | 多巴胺,抗污表面,氫氧基磷灰石,骨型態發生蛋白, | zh_TW |
dc.subject.keyword | dopamine,anti-fouling,hydroxyapatite,bone morphogenetic protein, | en |
dc.relation.page | 179 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-12 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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ntu-102-1.pdf 目前未授權公開取用 | 5.25 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。