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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李承叡(Cheng- Ruei Lee) | |
| dc.contributor.author | Yuan-Yun Zhang | en |
| dc.contributor.author | 張淵云 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:39:48Z | - |
| dc.date.available | 2025-06-24 | |
| dc.date.copyright | 2020-07-17 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-07-08 | |
| dc.identifier.citation | 1. 朱兆雲。2008。滇南本草。雲南科技出版社。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60983 | - |
| dc.description.abstract | 綬草(Spiranthes sinensis (Pers.) Ames)為蘭科綬草屬之多年生小型地生蘭，因為花序呈現螺旋狀型似綬帶而得名，其根部簇生且粗大形象與人蔘相似且花序旋生如盤龍繞柱而且可入藥，故亦稱盤龍蔘。分佈廣泛，可見於濕潤草生地，而各項性狀於不同地區都有較為明顯的差異。外型特殊，常作為觀賞植物，並亦為草藥，其代謝物具有良好的抗氧化性，其中阿魏酸二十八醇酯(Octacosyl Ferulate)已被證實有抗腫瘤作用。其種子缺乏胚乳需與真菌共生無法自行發芽因而栽培不易。近來因過度採集與棲地破壞已被列入《瀕危野生動植物物種國際貿易公約》（CITES）附錄Ⅱ中。本研究取臺灣各區域之綬草為材料，並從多個面向了解臺灣綬草多樣性。本研究於野外採集區域包括台北、新北、新竹、台中、嘉義、台南、台東和花蓮，十個位點共八十個體。從遺傳面向，利用流式細胞術分析綬草基因組大小發現並無明顯差異皆落在約9GB，但從染色體計數發現台灣各地區綬草染體數有所不同，有26、28與30三種。而在葉綠體基因組中trnS-G基因，做演化樹比較族群分化並可將台灣島上的綬草分為兩群，此分群基於trnS-G基因不同的單倍型鹼基T或G，另外還發現到單倍型為G的個體皆擁有26條染色體，大多數為T的個體則為30條，而擁有28條染色體的個體則可能為兩者之雜交。各地區代謝物構成及含量亦有所不同，我們更發現花蓮鯉魚山的族群其萃取液抗氧化力高於其他族群，而於觀測綬草生殖模式中也發現少數個體或可行無融合生殖。綜合以上結果可以得知綬草在台灣島上之各族群間亦存在相當之多樣性。 | zh_TW |
| dc.description.abstract | Spiranthes sinensis (Pers.) Ames is widely distributed in Asia and Oceania. Its traits have obvious differences in different country. It can be used as an ornamental plant and also as a traditional Chinese medicine. Because of overexploitation and habitat destruction in recent years, it became scarce. This study takes samples from various regions of Taiwan, aiming to understand the population variation of Spiranthes sinensis (Pers.) Ames in order to provide the basis for conservation. My field collection sites include Taipei, New Taipei, Hsinchu, Taichung, Chiayi, Tainan, Taitung and Hualien, with more than 80 individuals in total. There was no significant difference in population samples by flow cytometry in terms of genome size, about 9 GB. However, the number of chromosomes differs among individuals, ranging from 26 to 30. By using the sequence of chloroplast gene trnS-G ten geographical groups could be partitioned into two clades and it based on one SNP in trnS-G locus. Moreover, we discovered that all individuals with the G haplotype in chloroplast trnS-G locus have 26 chromosomes, and most individuals with the T haplotype have 30 chromosomes. Some individuals have 28 chromosomes, likely the hybrid between the two types. The composition and content of secondary metabolites are different among populations. It was found that the extract of Hualien Liyu mountain had highest antioxidant capacity. Observing the reproductive model of Spiranthes sinensis (Pers.) Ames, we also found that a few individuals show potentials for apomixis. All in all, it could be recognized that variability of Spiranthes sinensis (Pers.) Ames in Taiwan was high. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:39:48Z (GMT). No. of bitstreams: 1 U0001-0107202016125800.pdf: 3743470 bytes, checksum: fcc6373e01e4c8f4e0a39f98a5fa04db (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 摘要 ------------------------------------------------------------------------------------------ i Abstract -------------------------------------------------------------------------------------- ii Contents -------------------------------------------------------------------------------------- iii Contents of Figures ------------------------------------------------------------------------- v Contents of Tables -------------------------------------------------------------------------- vii Introduction --------------------------------------------------------------------------------- 1 Materials and Methods --------------------------------------------------------------------- 5 Sample collection -------------------------------------------------------------------- 5 Determination of genome size------------------------------------------------------- 5 Chromosome counting--------------------------------------------------------------- 5 DNA Extraction, PCR, Sequencing and Phylogenetic analysis----------------- 6 Analysis of secondary metabolites variances-------------------------------------- 7 Oxidation resistance of The extracts ----------------------------------------------- 8 Paraffin section of flowers and fruits for reproduction mode------------------ 9 Results --------------------------------------------------------------------------------------- 10 Genome size and variation---------------------------------------------------------- 10 Genetic variation --------------------------------------------------------------------- 11 Secondary metabolite variation----------------------------------------------------- 12 Reproduction mode------------------------------------------------------------------- 14 Discussion ----------------------------------------------------------------------------------- 16 References ----------------------------------------------------------------------------------- 22 Figures --------------------------------------------------------------------------------------- 26 Tables ---------------------------------------------------------------------------------------- 42 | |
| dc.language.iso | en | |
| dc.title | 臺灣綬草族群差異 | zh_TW |
| dc.title | population variation of Spiranthes sinensis (Pers.) Ames in Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 胡哲明(Jer-Ming Hu),王俊能(Chun-Neng Wang),陳賢明(Hieng-Ming Ting) | |
| dc.subject.keyword | 綬草,繁殖模式,染色體,遺傳多樣性,代謝物差異, | zh_TW |
| dc.subject.keyword | Spiranthes sinensis (Pers.) Ames,Chromosomes,genetic diversity,metabolites,reproductive model, | en |
| dc.relation.page | 49 | |
| dc.identifier.doi | 10.6342/NTU202001245 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2020-07-09 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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