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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 沈弘俊(Horn-Jiunn Sheen) | |
dc.contributor.author | Huang-Min Chu | en |
dc.contributor.author | 朱晃民 | zh_TW |
dc.date.accessioned | 2021-06-16T10:32:22Z | - |
dc.date.available | 2018-08-27 | |
dc.date.copyright | 2013-08-27 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-14 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60836 | - |
dc.description.abstract | 從1980年代以來生物晶片迅速發展,世界各國大學、研究機構致力於相關技術與生物晶片應用開發,實驗室晶片(Lab-on-a-chip)為一微型化晶片,可以在短時間內同步完成大量分析研究,本研究成功開發氣壓操控液珠3D微流道晶片,以應用於蘭花病毒檢測為例,氣壓操控液珠3D微流道晶片可對齒舌蘭輪斑病毒(ORSV)樣品輸送、混合與檢測,檢測機制是以螢光粒子布朗運動為理論基礎,有別於以往只能檢測單一樣本,使用氣壓操控液珠3D微流道晶片在顯微鏡下以CCD擷取畫面,再以微粒子追蹤測速儀(μ-PTV)針對螢光粒子布朗運動做速度分析,可進行多樣本檢測(Multi-sample detection)縮減檢測時間,判別樣本內是否存在齒舌蘭輪斑病毒外亦能分析出病毒濃度區間,檢驗流程簡單且成本低廉,有機會成為國際上常態使用的蘭花病毒檢驗晶片。 | zh_TW |
dc.description.abstract | We have successfully developed a pressure-controlled droplet-based 3D microfluidic device on simultaneous multiple sample detection. The device includes sample mixing, delivering and loading and is applied to detecting the ORSV of the samples by measuring nano-particle's Brownian velocities. As the functionalized nanoparticles react with different samples, the motions of these nanoparticles are recorded and then analyzed by micro-PTV. This lab-on-a-chip device owning the capability of multiple sample detection can shrink the investigating time when working on multiple sample detection. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T10:32:22Z (GMT). No. of bitstreams: 1 ntu-102-R00543075-1.pdf: 5027961 bytes, checksum: 4102d1d2a3476f45102ff6c886023306 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 誌謝 ii
中文摘要 iii ABSTRACT iv 目錄 v 圖目錄 viii 表目錄 x 符號目錄 xi 第一章 緒論 1 1.1 前言 1 1.2 研究動機與目的 2 1.3 研究方法 3 1.4 論文架構 4 第二章 文獻回顧 5 2.1 常見的蘭花病毒 5 2.2 蘭花病毒檢測方法與裝置 7 2.2.1 指示植物生物檢定法 7 2.2.2 電子顯微鏡觀察法 8 2.2.3 光學顯微鏡觀察法 8 2.2.4 抗血清檢定法 8 2.2.5 聚合酶連鎖反應(Polymerase chain reaction, 簡稱PCR) 10 2.2.6 即時定量PCR(Quantitative PCR) 12 2.2.7 生物晶片檢測 12 2.2.8 互補核酸探針(Complimentary DNA probe) 13 2.3 布朗運動文獻回顧 13 第三章 布朗運動量測實驗理論與方法 15 3.1 布朗運動概述 15 3.1.1 前言 15 3.1.2 愛因斯坦布朗運動數學模型 16 3.1.3 朗之萬布朗運動數學模型 20 3.1.4 膠體粒子的布朗運動擴散與沉降平衡 22 3.2 微粒子影像/追蹤測速技術工作原理 24 第四章 實驗設備架構與實驗步驟 27 4.1 微粒子影像/追蹤測速儀實驗設備 27 4.1.1 光源裝置 27 4.1.2 影像擷取裝置 27 4.1.3 光學顯微鏡 28 4.1.4 同步器 30 4.1.5 影像分析軟體 30 4.2 抗體自組裝與螢光粒子 30 4.2.1 螢光粒子選用 30 4.2.2 抗體自組裝於螢光粒子表面實驗 31 4.3 3D微流道檢測晶片 34 4.3.1 流道設計 34 4.3.2 SU-8 母模製作 37 4.3.3 PDMS 翻模製程 38 4.4 氣壓推動樣品輸送混合實驗 39 4.5 影像擷取裝置視野校正 40 4.6 粒徑變化驗證實驗 40 4.7 氣壓推動ORSV病毒樣本檢測 41 4.7.1 使用雙玻片夾層腔體檢測ORSV 41 4.7.2 使用氣壓操控液珠3D微流道晶片檢測ORSV 42 第五章 實驗結果與討論 43 5.1 氣壓推動樣品混合實驗結果 43 5.2 影像擷取裝置視野校正 48 5.3 粒徑變化驗證實驗結果 49 5.4 使用雙玻片夾層腔體檢測ORSV 52 5.5 使用氣壓操控液珠3D微流道晶片檢測ORSV 54 第六章 研究結論與未來展望 61 6.1 研究結論 61 6.2 未來展望 62 Refferences 63 | |
dc.language.iso | zh-TW | |
dc.title | 氣壓操控液珠3D微流道晶片用於齒舌蘭輪斑病毒樣品輸送、混合與檢測 | zh_TW |
dc.title | Pressure-controlled Droplet-based 3D Microfluidic Device for ORSV Sample Deliver, Mixing, and Detection | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳光鐘(Kuang-Chong Wu),張雅君(Ya-Chun Chang),黃榮山(Long-Sun Huang) | |
dc.subject.keyword | 實驗室晶片,微流道,齒舌蘭輪斑病毒,多樣本檢測,布朗運動,微粒子追蹤測速儀, | zh_TW |
dc.subject.keyword | Lab-on-a-chip,microfluidic device,ORSV,Multiple sample detection,Brownian motion,μ-PTV, | en |
dc.relation.page | 61 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-14 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 應用力學研究所 | zh_TW |
顯示於系所單位: | 應用力學研究所 |
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