以靜電紡絲法製備透明質酸/幾丁聚醣/聚乙烯醇複合奈米纖維之研究

Abstract

許多研究已證實，含有透明質酸的複合生醫材料有助於細胞培養。由於幾丁聚醣和透明質酸有著生物相容性高、易於改質等優點，若製成和胞外間質結構相似的奈米纖維，可增加其在生醫領域裡的應用。因此本研究目標為以靜電紡絲法製作透明質酸、幾丁聚醣和聚乙烯醇的三成分複合奈米纖維膜。實驗中以不同濃度和比例的三成分溶液來搭配流量、電壓的改變，來尋找適合靜電紡絲的參數範圍。 根據實驗結果，由電子顯微鏡和黏度分析，可知黏度明顯影響靜電紡絲的製程。熱化學分析結果證實透明質酸和幾丁聚醣為均勻物理性混合，無明顯相分離產生，紅外線吸收光譜也顯示官能基並未有異動。總和以上所述，本研究成功地將透明質酸、幾丁聚醣和聚乙烯醇製成奈米纖維膜，希冀未來可應用於組織工程等相關科技中。

Until today, many studies have proven that attachment and proliferation of cells can be enhanced by adding hyaluronan into the experimental materials. With the advantages of good biocompatibility and functional group modification for both chitosan and hyaluronan, fabricating them into nanofibrous structures like extracellular matrix may improve their applications in biomedical field. So the aim of this research is to fabricate hyaluronan/chitosan/polyvinyl alcohol (HA/CS/PVA, or H/C/P) composite nanofibers by electrosinning. In following experiments, the material concentrations, material ratios, feed flow rates and applied voltages were varied to investigate the best parameters for electrospinning. According to the results, thermal analysis indicated that HA and CS in the fabricated composite nanofibrous mats were mixed homogeneously without obvious phase separation. The spectrum from FT-IR also showed no alteration of functional groups. In this research, the hyaluronan/chitosan/polyvinyl alcohol nanofibrous mats were successfully fabricated. It is expected that such composite mats can be applied in tissue engineering-related fields in the future.