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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 沈偉強(Shen Wei-chiang) | |
| dc.contributor.author | Bi-Hua Yang | en |
| dc.contributor.author | 楊碧華 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:30:42Z | - |
| dc.date.available | 2023-08-13 | |
| dc.date.copyright | 2013-08-20 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-15 | |
| dc.identifier.citation | Agerbirk, N., and Olsen, C.E. 2012. Glucosinolate structures in evolution. Phytochemistry 77:16-45.
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Molecular Nutrition & Food Research 54:127-135. Zhao, Z., Zhang, W., Stanley, B.A., and Assmann, S.M. 2008. Functional proteomics of Arabidopsis thaliana guard cells uncovers new stomatal signaling pathways. The Plant Cell Online 20:3210-3226. Zhou, C., Tokuhisa, J.G., Bevan, D.R., and Esen, A. 2012. Properties of β-thioglucoside hydrolases (TGG1 and TGG2) from leaves of Arabidopsis thaliana. Plant Science 191–192:82-92. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60799 | - |
| dc.description.abstract | 硫配醣體-芥子酶 (glucosinolate-myrosinase)系統，利用水解硫配醣體產生具毒性的化學物質，作為植物的一種非專一性化學防禦機制。芥子酶活性主要存在於十字花科植物中，目前已有許多物種被報導具有芥子酶活性，然而僅在少數植物及蚜蟲發表芥子酶基因之選殖。本研究利用阿拉伯芥及蚜蟲的芥子酶基因之蛋白質序列，在甘藍黑斑病原菌 (A. brassicicola) 基因庫進行序列比對，得到3個候選基因：AB03581，AB00050及 AB10392。進一步分析芥子酶胺基酸序列的親緣關係，發現AB03581與AB10392與阿拉伯芥非典型芥子酶PEN2及蚜蟲芥子酶的親緣較為相近，而AB00050則位於芥子酶親緣類群之外。利用生物資訊學工具進行蛋白質結構模擬分析，AB03581和AB00050都具有與芥子酶酵素相似的 (α/β)8筒狀蛋白質結構。而AB10392序列上的缺失，則造成筒狀結構的缺陷，推測AB10392 不具有功能。在黑芥子硫酸鉀 (sinigrin) 誘導實驗發現，經6小時誘導後，AB03581基因的表現量上升30倍。進一步地，將AB03581基因進行大腸桿菌異源表現，純化出AB03581蛋白質，成功地測得芥子酶活性，並將AB03581命名為AbMyr1基因。將AbMyr1純化蛋白質系列稀釋成1-6 μg/μL，滴在含有鋇及黑芥子硫酸鉀的培養基中，3 μg/μL 以上的蛋白質濃度皆可造成白色的沉澱。同時亦發現，額外添加Zn2+ 可以促進蛋白質的活性，而添加抗敗血酸 (ascorbate) 則對酵素沒有影響。 | zh_TW |
| dc.description.abstract | Glucosinolate-myrosinase system is a well-studied nonspecific chemical defense mechanism of plant by hydrolyzing glucosinolates to produce toxic substances. Myrosinase activities are widely found and studied in cruciferous plants, and also reported in some aphids, fungi, bacteria, and intestinal bacteria of mammals. However, myrosinase genes were identified only in plants and aphids. In this study, we utilized the sequences of Arabidopsis thaliana TGG gene family and PEN2, and Brevicoryne brassicae BMY1 protein to search the putative myrosinase genes in Alternaria brassicicola genome database. Three candidate genes including AB03581, AB00050 and AB10392 were identified. Phylogenetic analysis showed that AB03581 and AB10392 are more closely related to an atypical myrosinase PEN2 gene and aphid myrosinase BMY1 gene, whereas AB00050 fell out of the myrosinase major clade. Molecular modeling revealed that AB03581 and AB00050 were similar to other myrosinase proteins which display the (α/β)8-barrel structure when using protein structure simulation analysis. In contrast, due to truncation of N-terminal part, AB10392 lost the catalytic site of typical β-glucosidase, implying that AB10392 may be not functional. Gene expression studies showed that the expression level of AB03581 increased about 30 folds after 6 hours under sinigrin inductive condition. Finally, AB03581 gene was heterologously expressed in E. coli expression system and proteins were purified and shown to contain myrosinase activity and we therefore named the gene of A. brassicicola as AbMyr1. Serial dilutions of AbMYR1 purified protein from 1 to 6 μg/μL were spotted onto BSA plate and opaque precipitation was observed when the concentration was higher than 3 μg/μL at 37°C. Addition of Zn2+ enhanced the AbMyr1 enzymatic activity, whereas ascorbate showed no effect. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:30:42Z (GMT). No. of bitstreams: 1 ntu-102-R99633022-1.pdf: 2513544 bytes, checksum: 2826078cef0fc2800005aac1bf6e43da (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 中文摘要 i
Abstract ii Abbreviations iv List of figures viii List of tables ix Appendix x Chapter I Introduction 1 1.1 Glucosinolate- myrosinase defense system in plant 1 1.1.1 Structure of glucosinolate 1 1.1.2 Myrosinase and their function roles 2 1.2 Myrosinase activity in other organisms 7 1.2.1 Insect 7 1.2.2 Microorganism 9 Chapter II Materials and Methods 11 2.1 Strain and growth conditions 11 2.2 Myrosinase candidate genes analysis 11 2.2.1 Characterization of myrosinase genes in A. brassicicola 11 2.2.2 Alignment and phylogenetic tree 11 2.3 Myrosinase induction 12 2.4 RNA isolation and RT-PCR 12 2.5 Real-time PCR analyses 13 2.6 Protein expression from reconstructed Escherichia coli 13 2.6.1 Stains, vectors, enzymes and reagents 13 2.6.2 Construction of the recombination expression vectors 14 2.6.3 Expression plasmid transformation 15 2.6.4 Expression of fusion protein in E. coli 15 2.6.5 Protein purification 16 2.6.6 SDS-PAGE and Western blot 16 2.6.7 Protein sequencing 17 2.6.8 Protein identification 18 2.7 Protein activity assay 18 2.7.1 Barium sinigrin agar plate assay 18 2.7.2 Gas Chromatography-Mass Spectrometry (GC-MS) 19 Chapter III Results 20 3.1 Myrosinase activity assay in Alternaria brassicicola 20 3.2 Identification of the putative myrosinase genes in A. brassicicola 20 3.3 Both AB03581 and AB00050 have conserved region for recognizing glucose ring but not AB10392 21 3.4 AB03581 gene expression highly association with the substrate sinigrin 23 3.5 Expression and purification of His tag fused AB03581 24 3.6 AB03581 protein activity assay 26 Chapter IV Discussions 28 Figures and tables 32 Appendix 54 References 58 | |
| dc.language.iso | en | |
| dc.title | 甘藍黑斑病菌芥子酶基因選殖與功能特性之分析 | zh_TW |
| dc.title | Cloning and characterization of a gene encoding myrosinase in Alternaria brassicicola | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 劉瑞芬(Ruey-Fen Liou),王肇芬(Jaw-Fen Wang),楊健志(Chien-Chih Yang),呂廷璋(Ting-jang Lu) | |
| dc.subject.keyword | 硫配醣體,芥子酶,甘藍黑斑病病原真菌,AbMyr1基因,蛋白質異源表現, | zh_TW |
| dc.subject.keyword | glucosinolate,myrosinase,Alternaria brassicicola,AbMyr1 gene,heterologous protein expression, | en |
| dc.relation.page | 67 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-15 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
| 顯示於系所單位： | 植物病理與微生物學系 | |
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