請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60718
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 周宏農 | |
dc.contributor.author | Shih-Hsin Tai | en |
dc.contributor.author | 戴世昕 | zh_TW |
dc.date.accessioned | 2021-06-16T10:27:19Z | - |
dc.date.available | 2015-08-23 | |
dc.date.copyright | 2013-08-23 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-15 | |
dc.identifier.citation | 江永棉、王瑋龍、黃淑芳,1990。台灣海藻簡介。台灣省立博物館出版部,p157。
梁宏彥、蕭聖代,2007。台灣產蜈蚣藻的形態與分類。水試專訊,18: 16–18。 周宏農、陳珮瑜,2005。經濟海域重要藻類(紫菜、蜈蚣藻、石花菜)資源量評及藻床復育之分析。行政院農業委員會漁業屬九十四年度科技計畫研究報告。 馬凌波、王素娟、何培民,1995。大型經濟紅藻-蜈蚣藻類的室內人工育苗。上海水產大學學報,4: 93–97。 張澤宇、魏海霞、韓余香、王國書,2007。蜈蚣藻盤狀體誘導絲狀體及其採苗的初步研究。大連水產學院學報,22: 164–169。 堀輝三,1993。藻類的生活史集成 (褐藻、紅藻類)。2: 268–269。 趙自國,2008。三種大型海藻的早期發育研究。東北師範大學,博士論文。 楊嘉穎,2010。稀毛蜈蚣藻生活史及養殖育苗技術研究。國立台灣大學,碩士論文。 陳卉,2000。菩提藻 Grateloupia filicina (Lamouroux) C. Agardh的再生組織和四分孢子所形成的絲狀體之培養。國立台灣海洋大學水產養殖系碩士學位論文。 陳淑慧,1994。經濟海藻蜈蚣藻 (Grateloupia filicina C. Ag.) 培養條件之探討。1.環境因子:溫度、光度、光週期及震盪轉速對蜈蚣藻生長之影響;2.植物生長調節物質對蜈蚣藻生長之影響。國立台灣海洋大學水產養殖系碩士學位論文。 陳國治、徐有德、夏福祖、何若文,1985。實用海水藻類養殖技術。農業出版社出版。 曾呈奎、王素娟、劉思儉、郭宣鍨、張定民,1985。海藻栽培學。上海科學技術 29 出版社出版。 葉文吉,1970。台灣北部產翼枝菜之研究。國立台灣大學海洋研究所碩士論文。 黃聲蘋,1970。鹽度對正龍鬚菜(Gracilaria verrucosa)與石花菜(Gelidium japonicum)生長之影響及該兩種紅藻對無機氮鹽選擇吸收性之研究。國立台灣大學海洋研究所碩士論文。 黃淑芳,2003。台灣產海膜藻科系統分類與植物地理之研究。國立台灣博物館九十二年度研究成果報告書。 翁韶蓮,2001,菩提藻生長環境條件與氮源需求之探討,國立臺灣海洋大學,博士論文。 劉風賢、李偉新。1986。蜈蚣藻和繁枝蜈蚣藻孢子發生類型的研究。大陸水產學報,3: 281-287。 豬野俊平,1947。海藻的發生。北隆館出版社出版。 田林文郎,1947。 蜈蚣藻果孢子的發生型態。藻類,13: 71–75. 村上迪代、豬野俊平,1967。蜈蚣藻的四分孢子的發生型態。藻類,15: 61–67. 江永棉、周應浩,1977。長葉紫菜之培養─絲狀體越夏培養及殼孢子放出條件。台灣水產學會刊,5: 91–97。 Chiang Y. M., 1962. Marine algae of northern Taiwan (Rhodophyta). Taiwania 8: 143–165. Chiang Y. M., 1970. Morphological studies of red algae of the family Cryptonemiaceae. University of California publications in botany. 58: 1–95. Chiang Y. M., 1993. The developmental sequence of the marine red alga Gratelopoia filicina in culture. Journal of Applied Phycology 8: 231–237. Guiry M. D., Guiry G. M., 2010. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org (searched on 30 16 August 2010). Hannach G., Waaland J. R.,1989. Growth and morphology of young gametophytes of Porphyra abbottae: effects of environmental factor in culture. Journal Phycology 25: 247–254. Huang W., Fujita Y., 1997. Callus induction and thallus regereration of the red alga Meristotheca papulosa (Rhodophyta, Gigartinales). Botanica marina 40: 55–62. Iima M., Kinoshita T., Kawaguchi S., Migita S., 1995. Cultivation of Grateloupia acuminata (Halymeniaceae, Rhodophyta) by regeneration from cut fragments of basal crusts and upright thalli. Journal of Applied Phycology 7: 583–588. Ishikawa Y.,1984. New seaweed resources 2: Cultivation of Grateloupia filicins. Suisan no Kenkyu 3: 57–60. Lin S. M., Liang H. Y., Hommersand M. H., 2008. Two types of auxiliary cell ampullae in Grateloupia (Halymeniaceae, Rhodophyta), including G. taiwanensis sp. nov. and G. orientalis sp. nov. from Taiwan based on rbcL gene sequence analysis and cystocarp development. Journal of Phycology 44: 196–214. Lobban C. S., Harrison P. J., Duncan M. J., 1985. The physiological ecology of seaweeds. Cambridge University Press, Cambridge. 1–74. Migita S.,1992. Suizenjinori (Aphanothece tortuosa), Habanori (Endarachne binghamine) and Mukadenori (Grateloupia filicina). In Cultivation of Edible Alage in Japan 94-111. Santelices B., Alvarado J., 2006. Applying the concept of metamorphosis to the crustose-to-erect thallus transition of macroalgae. Integrative and Comparative Biology 46: 713–718. 31 Shao K. S., Wei J. X., Zho B. C., 2004. Production and application of filaments of Grateloupia turuturu (Halymeniaceae, Rhodophyta). Journal of Applied Phycology 16: 431–437. Strain L. W.S., Isdepsky Andreas, Borowitzka M. A., Daume S., 2007. Three algal propagation methods assessed to create a rhodophyta diet for juvenile greenlip abalone (Haliotis laevigata) in the later nursery phase. Journal of shellfish research 26: 737–744. Simon C., Gall E. Ar, Deslandes E., 2001. Expansion of the red alga Grateloupia doryphora along the coasts of Brittany (France). Hydrobiologia 443: 23–29. Sidirell-Wolff M., 1992.The influence of temperature, irradiance and photoperiod on the reprpductive life history of Porphyra leucosticte in laboratory culture. Botanica marina 35: 251–257. Wang S. C., Bligh S. W. A., Shi S. S., Wang Z. T., Hu Z. B., Crowder J., Branford-White C., Vella C., 2007. Structural features and anti-HIV-1 activity of novel polysaccharides from red algae Grateloupia longifolia and Grateloupia filicina. International Journal of Biological Macromolecules 41: 369–375. Wong S. L., Chang J., 2000. Salinity and light effects on growth, photosynthesis, and respiration of Grateloupia filicina (Rhodophyta). Aquaculture 182: 387–395. Yang H. N., Wamg. L. W., Liao. M. L., 1994. Marine algae flora of Pengahia Yu and its special place in the marine phytogeography of Taiwan. Botanica Marina 37: 429–432. Yokoya N. S., Oliverira E. C., 1992. Temperature responses economically important red algae and their potential for mariculture in Brazilian waters. Journal of Applied Phycology 4: 339–345. 32 Zablackis E., 1987. The effect of salinity on growth rate amd branch morphology in tank cultivate Grateloupia filicina in Hawaii. Botany 27: 187–193. Zablackis E., Perez J., 1990. A partially pyruvated carrageenan from Hawaiian Grateloupia filicina (Cryptonemiales, Rhodophyta). Botanica Marina 33: 273–276. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60718 | - |
dc.description.abstract | 蜈蚣藻屬於紅藻門 (Rhodophyta)、海膜藻目 (Halymeniales)下的一個屬,為大型經濟海藻之一,近幾年已廣泛地被利用為生鮮食品,臺灣東北角沿岸潮間及潮下帶為主要生長與人工採集地域,靠著少數業者藉潛水方式於野外摘採,無法大量供應市場需求,但卻鮮少人研究其大量養殖的方法。本研究為建立G. taiwanensis的種苗和培育條件以供大量養殖所需,開發出蜈蚣藻室內育苗技術,使用經由孢子發芽成未貼附盤狀體所形成的結節絲狀體,透過保種、放大培養方式來保存可資繁殖成葉狀體的種源。可隨時利用結節絲狀體打碎後的藻液噴於網具與培養皿靜置培養,使長成盤狀體後,再經低溫(20℃)及高光照(7000lux)刺激使其在基質上誘發幼苗,待小芽成長1公分至秋冬氣候來臨時,移置戶外促使葉狀體在自然露天環境下育成,並能大量養殖。而在研究中發現雙倍結節絲狀體比單倍結節絲狀體喜好較高的溫度;而比較果孢子及四分孢子的附苗情況,作為最適附苗的操作流程,其中發現源自果孢子的雙倍體結節絲狀體與果孢子發芽形成盤狀體較小,容易導致幼苗脫落,附苗效果較差,因而轉換開發懸浮培養的可能性,結果確能在生長季節內成長至10~20公分長之藻葉片,然而型態與漁網附苗長成之葉片有顯著差異。後續將調整加速室內量化育苗時間及數量,確定懸浮苗的生長季節適應,並由人工培養時間確立生活史自然生長的時軸進行養殖技術的最後確立。 | zh_TW |
dc.description.abstract | Grateloupia is a commercial macroalgal which belonging to the phyla of Rhodophyta under the order of Halymeniales. Field collection from the intertidal zone of Taiwan North-eastern coast, Grateloupia has been widely consumed as a delicacy by the local people. Wild Grateloupia was hand-picked by snorkelers in an inefficient way. Thus, the production of Grateloupia was limited and unable to meet the demand from the market. Despite this fact, very few researches on the cultivation of Grateloupia were done on the production of Grateloupia at commercial scale. In this study, the propagation of Grateloupia taiwanensis nodulous filament for germ stock and seedling development was investigated. The nodulous filaments were prepared from germinating carporspores and tetraspores separately. Discoid crust from germinating spores were detached from the substrat, and maintained in enriched medium. Subsequently, various conditions to scale-up the production of nodulus filament cultures were determined. The nodulus filaments were blended into small fragments which were capable in forming new discoid crusts under appropriate conditions. At 20℃, under high illumination of light (7000 lux), young shoots formed from the crusts. The young shoots were transferred to outdoor environment for further growth under appropriate conditions. It was also observed that diploid nodulus filaments germinated from carpospores grew better at higher temperature compared to the haploid nodulous filaments, which were germinated from tetraspores. Most of the discoid crusts formed from diploid nodulous filaments and carpospores were smaller in size, and were easily detached from substrate. Therefore, we suggest that nodulus filaments from detached diploid discoid crusts is an ideal source of seedlings which can be cultivated in suspension. During its growing season, the length of thalli developed from the diploid nodulus filaments reached about 10-20 cm. However, the morphology of the thallus is significantly different from those collected from the field. We suggest that the indoor cultivation of seedling sources to be improved at larger scale. The life cycle of Grateloupia may also can be determined artificially using this technique. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T10:27:19Z (GMT). No. of bitstreams: 1 ntu-102-R00b45014-1.pdf: 2097943 bytes, checksum: 6ea1c9ced5d9dd74c5da56f099242231 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 中文摘要……………………………………………………………………………… І
英文摘要……………………………………………………………………………… П 第一章前言………………………………………………………………………… 1 第二章文獻回顧 2-1繁養殖經濟海藻的方式……………………………………………4 2-2 蜈蚣藻人工繁養殖現況…………………………………………………………4 2-3蜈蚣藻應用價值………………………………………………………………6 2-4蜈蚣藻孢子型態的觀察………………………………………………………7 2-5 蜈蚣藻絲狀體型態的描述…………………………………………………7 2-5 環境因子對於海藻生長及發育的影響……………………………………8 2-6 誘發葉狀體的關鍵……………………………………………………………9 第三章材料與方法 3-1 藻類材料與來源…………………………………………………………… 10 3-2 孢子萌發實驗 3-2-1葉狀體前處理……………………………………………………… 10 3-3-2 果孢子與四分孢子的釋放………………………………………… 10 3-3 不同世代絲狀體的培養操作及維持……………………………………… 11 3-3-1不同溫度與光照下對N及2N結節絲狀體之成長比較……………… 11 3-3-2不同光週期下對N及2N結節絲狀體之成長比較…………………… 12 3-4 不同世代盤狀體的培養與操作……………………………………………… 12 3-4-1不同溫度對N及2N盤狀體之附苗比較……………………………… 12 3-4-2不同光強度對N及2N盤狀體之附苗比較…………………………… 13 3-4-3不同光週期對N及2N盤狀體之附苗比較…………………………… 13 3-5室內誘發2N懸浮葉狀體的操作流程……………………………………… 14 VII 3-5-1不同溫度與光照下對2N懸浮葉狀體之成長比較…………………… 14 3-5-2不同光週期2N懸浮葉狀體之成長比較…………………………… 14 3-6 種苗繁殖……………………………………………………………………15 3-6-1雌配子體種苗培養……………………………………………………15 3-6-2 四分胞子體種苗……………………………………………………15 3-7田間放養實驗…………………………………………………………………15 3-8統計方式………………………………………………………………………16 第四章結果與討論 4-1 野外不同世代葉狀體的觀察與計數結果 4-1-1 葉狀體內部結構…………………………………………………… 17 4-1-2 孢子萌發條件測試結果…………………………………………… 17 4-2 不同來源絲狀體的培養及成長比較 4-2-1 型態觀察…………………………………………………………… 18 4-2-2結節絲狀體溫度成長比較………………………………………… 18 4-2-3結節絲狀體光週期成長比較………………………………………… 19 4-3不同來源盤狀體的培養及生長比較………………………………………… 20 4-3-1溫度成長比較………………………………………………………… 20 4-3-2光強度成長比較……………………………………………………… 20 4-3-2光週期成長比較……………………………………………………… 21 4-4 2N懸浮葉狀體的溫度與光照生長比較……………………………………… 21 4-4-1光週期成長比較……………………………………………………… 22 4-5 種苗培養………………………………………………………………………22 4-6雙倍體孢子體之懸浮培養的成長與人工養殖操作………………………………23 4-7量化配子體蜈蚣藻種苗……………………………………………………………23 4-8雙倍體孢子體之懸浮培養的成長與人工養殖操作………………………………24 4-9戶外蜈蚣藻的生長季節探討………………………………………………………25 VIII 第五章參考文獻………………………………………………………………28 圖目錄 Fig 1. 臺灣蜈蚣藻不同世代藻體外觀及內部結構………………………………… 33 Fig 2. N結節絲狀體在不同溫度搭配不同光強度的成長比較…………………… 34 Fig 3. 2N結節絲狀體在不同溫度搭配不同光強度的成長比較………………… 35 Fig 4. N、2N結節絲狀體在不同光週期下的成長比較………………………… 36 Fig 5. N盤狀體及2N盤狀體發育觀察………………………………………… 37 Fig 6. N、2N結節絲狀體在不同溫度下的附苗條件比較………………………… 38 Fig 7. N、2N結節絲狀體在不同溫度下的附苗成長比較………………………… 39 Fig 8. N、2N結節絲狀體在不同光強度下的附苗條件比較……………………… 40 Fig 9. N、2N結節絲狀體在不同光強度下的附苗成長比較……………………… 41 Fig 10. N、2N結節絲狀體在不同光週期下的附苗條件比較……………………… 42 Fig 11. N、2N結節絲狀體在不同光週期下的附苗成長比較……………………… 43 Fig 12. 2N懸浮葉狀體在不同溫度搭配二種不同光強度的成長比較………………44 Fig 13. 2N懸浮葉狀體在不同光週期下的成長比較……………………………… 45 Fig 14. 2N結節絲狀體小量附苗及育苗實驗……………………………………… 46 Fig 15. 雙倍體孢子體之懸浮培養的成長與人工養殖操作…………………………47 Fig 16. 單倍雌配子體附苗培養操作流程………………………………………… 48 Fig 17. 田間大量育苗成果…………………………………………………………… 49 Fig 18. 戶外蜈蚣藻放養全年生長季節探討………………………………………… 50 Fig. 19. 臺灣蜈蚣藻生活史各世代存在季節之推論圖……………………………… 51 IX 附件目錄 附錄一、澎湖天和箱網養殖場地理位置…………………………………………… 52 附錄二、SWM-III 培養基成份……………………………………………………… 53 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣蜈蚣藻葉狀孢子體與配子體之不同世代的人工養殖育苗技術研究 | zh_TW |
dc.title | Aquaculture Development of different generation of Thalloid Sporophyte and Gametophyte of Grateloupia taiwanensis | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃淑芳,林?美,李宗徽 | |
dc.subject.keyword | 臺灣蜈蚣藻,結節絲狀體,生活史,種苗, | zh_TW |
dc.subject.keyword | Grateloupia taiwanensis,Nodulous filaments,Lifecycle,Seedling, | en |
dc.relation.page | 53 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-15 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf 目前未授權公開取用 | 2.05 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。