請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60648完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 呂俊宏(June-Horng Lue) | |
| dc.contributor.author | Ya-Tang Chen | en |
| dc.contributor.author | 陳雅棠 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:24:39Z | - |
| dc.date.available | 2013-09-24 | |
| dc.date.copyright | 2013-09-24 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-15 | |
| dc.identifier.citation | Adachi J, Yoshioka N, Sato M, Nakagawa K, Yamamoto Y, Ueno Y (2005) Detection of phosphatidylcholine oxidation products in rat heart using quadrupole time-of-flight mass spectrometry. Journal of chromatography B, Analytical technologies in the biomedical and life sciences 823:37-43.
Alier KA, Chen Y, Sollenberg UE, Langel U, Smith PA (2008) Selective stimulation of GalR1 and GalR2 in rat substantia gelatinosa reveals a cellular basis for the anti- and pro-nociceptive actions of galanin. Pain 137:138-146. Allt G, Ghabriel MN, Sikri K (1988) Lysophosphatidyl choline-induced demyelination. A freeze-fracture study. Acta neuropathologica 75:456-464. Aoki J (2004) Mechanisms of lysophosphatidic acid production. Seminars in cell & developmental biology 15:477-489. Aoki Y, An HS, Takahashi K, Miyamoto K, Lenz ME, Moriya H, Masuda K (2007) Axonal growth potential of lumbar dorsal root ganglion neurons in an organ culture system: response of nerve growth factor-sensitive neurons to neuronal injury and an inflammatory cytokine. Spine 32:857-863. Bao L, Wang HF, Cai HJ, Tong YG, Jin SX, Lu YJ, Grant G, Hokfelt T, Zhang X (2002) Peripheral axotomy induces only very limited sprouting of coarse myelinated afferents into inner lamina II of rat spinal cord. Eur J Neurosci 16:175-185. Barreda-Gomez G, Giralt MT, Rodriguez-Puertas R (2005) G protein-coupled galanin receptor distribution in the rat central nervous system. Neuropeptides 39:153-156. Barreto SG, Bazargan M, Zotti M, Hussey DJ, Sukocheva OA, Peiris H, Leong M, Keating DJ, Schloithe AC, Carati CJ, Smith C, Toouli J, Saccone GT (2011) Galanin receptor 3--a potential target for acute pancreatitis therapy. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 23:e141-151. Bartfai T, Hokfelt T, Langel U (1993) Galanin--a neuroendocrine peptide. Critical reviews in neurobiology 7:229-274. Burazin TC, Larm JA, Ryan MC, Gundlach AL (2000) Galanin-R1 and -R2 receptor mRNA expression during the development of rat brain suggests differential subtype involvement in synaptic transmission and plasticity. The European journal of neuroscience 12:2901-2917. Cheng HT, Dauch JR, Porzio MT, Yanik BM, Hsieh W, Smith AG, Singleton JR, Feldman EL (2013) Increased Axonal Regeneration and Swellings in Intraepidermal Nerve Fibers Characterize Painful Phenotypes of Diabetic Neuropathy. The journal of pain : official journal of the American Pain Society. Cizkova D, Lukacova N, Marsala M, Marsala J (2002) Neuropathic pain is associated with alterations of nitric oxide synthase immunoreactivity and catalytic activity in dorsal root ganglia and spinal dorsal horn. Brain research bulletin 58:161-171. Coderre TJ, Katz J, Vaccarino AL, Melzack R (1993) Contribution of central neuroplasticity to pathological pain: review of clinical and experimental evidence. Pain 52:259-285. Cridland RA, Henry JL (1988) Effects of intrathecal administration of neuropeptides on a spinal nociceptive reflex in the rat: VIP, galanin, CGRP, TRH, somatostatin and angiotensin II. Neuropeptides 11:23-32. Day AS, Lue JH, Sun WZ, Shieh JY, Wen CY (2001) A beta-fiber intensity stimulation of chronically constricted median nerve induces c-fos expression in thalamic projection neurons of the cuneate nucleus in rats with behavioral signs of neuropathic pain. Brain research 895:194-203. Devor M (1991) Neuropathic pain and injured nerve: peripheral mechanisms. British medical bulletin 47:619-630. Eder AM, Sasagawa T, Mao M, Aoki J, Mills GB (2000) Constitutive and lysophosphatidic acid (LPA)-induced LPA production: role of phospholipase D and phospholipase A2. Clinical cancer research : an official journal of the American Association for Cancer Research 6:2482-2491. Floren A, Land T, Langel U (2000) Galanin receptor subtypes and ligand binding. Neuropeptides 34:331-337. Hamann W, Abou-Sherif S, Thompson S, Hall S (2006) Pulsed radiofrequency applied to dorsal root ganglia causes a selective increase in ATF3 in small neurons. European journal of pain 10:171-176. Helliwell RJ, McLatchie LM, Clarke M, Winter J, Bevan S, McIntyre P (1998) Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia. Neuroscience letters 250:177-180. Herzberg U, Brown DR, Mullett MA, Beitz AJ (1996) Increased delayed type hypersensitivity in rats subjected to unilateral mononeuropathy is mediated by neurokinin-1 receptors. Journal of neuroimmunology 65:119-124. Hulse RP, Donaldson LF, Wynick D (2012) Peripheral galanin receptor 2 as a target for the modulation of pain. Pain research and treatment 2012:545386. Hunt SP, Pini A, Evan G (1987) Induction of c-fos-like protein in spinal cord neurons following sensory stimulation. Nature 328:632-634. Inoue M, Rashid MH, Fujita R, Contos JJ, Chun J, Ueda H (2004) Initiation of neuropathic pain requires lysophosphatidic acid receptor signaling. Nat Med 10:712-718. Ivanavicius SP, Ball AD, Heapy CG, Westwood FR, Murray F, Read SJ (2007) Structural pathology in a rodent model of osteoarthritis is associated with neuropathic pain: increased expression of ATF-3 and pharmacological characterisation. Pain 128:272-282. Jimenez-Andrade JM, Lundstrom L, Sollenberg UE, Langel U, Castaneda-Hernandez G, Carlton SM (2006) Activation of peripheral galanin receptors: differential effects on nociception. Pharmacology, biochemistry, and behavior 85:273-280. Jimenez-Andrade JM, Zhou S, Du J, Yamani A, Grady JJ, Castaneda-Hernandez G, Carlton SM (2004) Pro-nociceptive role of peripheral galanin in inflammatory pain. Pain 110:10-21. Lapsys NM, Furler SM, Henderson NK, Dutton JL, Hort YJ, Eisman JA, Shine J, Iismaa TP (1999) A polymorphism in the human GALR3 galanin receptor gene (GALNR3). Molecular and cellular probes 13:325-327. Lee Y, Kawai Y, Shiosaka S, Takami K, Kiyama H, Hillyard CJ, Girgis S, MacIntyre I, Emson PC, Tohyama M (1985) Coexistence of calcitonin gene-related peptide and substance P-like peptide in single cells of the trigeminal ganglion of the rat: immunohistochemical analysis. Brain research 330:194-196. Lin CT, Wang HY, Tsai YJ, Huang CT, Chen SH, Lue JH (2009) Pre-treatment with lidocaine suppresses ectopic discharges and attenuates neuropeptide Y and c-Fos expressions in the rat cuneate nucleus following median nerve transection. Journal of chemical neuroanatomy 38:47-56. Liu HX, Brumovsky P, Schmidt R, Brown W, Payza K, Hodzic L, Pou C, Godbout C, Hokfelt T (2001) Receptor subtype-specific pronociceptive and analgesic actions of galanin in the spinal cord: selective actions via GalR1 and GalR2 receptors. Proceedings of the National Academy of Sciences of the United States of America 98:9960-9964. Lue JH, Leong SM, Day AS, Tsai YJ, Shieh JY, Wen CY (2002) Changes in c-Fos protein expression in the rat cuneate nucleus after electric stimulation of the transected median nerve. Journal of neurotrauma 19:897-907. Marchand JE, Cepeda MS, Carr DB, Wurm WH, Kream RM (1999) Alterations in neuropeptide Y, tyrosine hydroxylase, and Y-receptor subtype distribution following spinal nerve injury to rats. Pain 79:187-200. Ma W, Bisby MA (2000) Partial sciatic nerve ligation induced more dramatic increase of neuropeptide Y immunoreactive axonal fibers in the gracile nucleus of middle-aged rats than in young adult rats. Journal of neuroscience research 60:520-530. Manassero G, Repetto IE, Cobianchi S, Valsecchi V, Bonny C, Rossi F, Vercelli A (2012) Role of JNK isoforms in the development of neuropathic pain following sciatic nerve transection in the mouse. Molecular pain 8:39. Marchand JE, Cepeda MS, Carr DB, Wurm WH, Kream RM (1999) Alterations in neuropeptide Y, tyrosine hydroxylase, and Y-receptor subtype distribution following spinal nerve injury to rats. Pain 79:187-200. Morgan JI, Curran T (1989) Calcium and proto-oncogene involvement in the immediate-early response in the nervous system. Annals of the New York Academy of Sciences 568:283-290. Nishimori T, Buzzi MG, Chudler EH, Poletti CE, Moskowitz MA, Uhl GR (1990) Preproenkephalin upregulation in nucleus caudalis: high and low intensity afferent stimulation differentially modulate early and late responses. The Journal of comparative neurology 302:1002-1018. O'Donnell D, Ahmad S, Wahlestedt C, Walker P (1999) Expression of the novel galanin receptor subtype GALR2 in the adult rat CNS: distinct distribution from GALR1. The Journal of comparative neurology 409:469-481. Ohara S, Roth KA, Beaudet LN, Schmidt RE (1994) Transganglionic neuropeptide Y response to sciatic nerve injury in young and aged rats. Journal of neuropathology and experimental neurology 53:646-662. Ohtori S, Miyagi M, Takaso M, Inoue G, Orita S, Eguchi Y, Ochiai N, Kishida S, Kuniyoshi K, Nakamura J, Aoki Y, Ishikawa T, Arai G, Kamoda H, Suzuki M, Toyone T, Takahashi K (2012) Differences in damage to CGRP immunoreactive sensory nerves after two lumbar surgical approaches: investigation using humans and rats. Spine 37:168-173. Pirondi S, Fernandez M, Schmidt R, Hokfelt T, Giardino L, Calza L (2005) The galanin-R2 agonist AR-M1896 reduces glutamate toxicity in primary neural hippocampal cells. Journal of neurochemistry 95:821-833. Sadegh M, Mirnajafi-Zadeh J, Javan M, Fathollahi Y, Mohammad-Zadeh M, Jahanshahi A, Noorbakhsh SM (2007) The role of galanin receptors in anticonvulsant effects of low-frequency stimulation in perforant path-kindled rats. Neuroscience 150:396-403. Shen Z, Belinson J, Morton RE, Xu Y, Xu Y (1998) Phorbol 12-myristate 13-acetate stimulates lysophosphatidic acid secretion from ovarian and cervical cancer cells but not from breast or leukemia cells. Gynecologic oncology 71:364-368. Sten Shi TJ, Zhang X, Holmberg K, Xu ZQ, Hokfelt T (1997) Expression and regulation of galanin-R2 receptors in rat primary sensory neurons: effect of axotomy and inflammation. Neuroscience letters 237:57-60. Stenberg L, Kanje M, Dolezal K, Dahlin LB (2012) Expression of activating transcription factor 3 (ATF 3) and caspase 3 in Schwann cells and axonal outgrowth after sciatic nerve repair in diabetic BB rats. Neuroscience letters 515:34-38. Strassman AM, Vos BP (1993) Somatotopic and laminar organization of fos-like immunoreactivity in the medullary and upper cervical dorsal horn induced by noxious facial stimulation in the rat. The Journal of comparative neurology 331:495-516. Strassman AM, Vos BP, Mineta Y, Naderi S, Borsook D, Burstein R (1993) Fos-like immunoreactivity in the superficial medullary dorsal horn induced by noxious and innocuous thermal stimulation of facial skin in the rat. Journal of neurophysiology 70:1811-1821. Szallasi A, Szabo T, Biro T, Modarres S, Blumberg PM, Krause JE, Cortright DN, Appendino G (1999) Resiniferatoxin-type phorboid vanilloids display capsaicin-like selectivity at native vanilloid receptors on rat DRG neurons and at the cloned vanilloid receptor VR1. British journal of pharmacology 128:428-434. Takemura M, Shimada T, Sugiyo S, Nokubi T, Shigenaga Y (2000) Mapping of c-Fos in the trigeminal sensory nucleus following high- and low-intensity afferent stimulation in the rat. Experimental brain research Experimentelle Hirnforschung Experimentation cerebrale 130:113-123. Tanaka T, Sano R, Yamashita Y, Yamazaki M (2004) Shape changes and vesicle fission of giant unilamellar vesicles of liquid-ordered phase membrane induced by lysophosphatidylcholine. Langmuir : the ACS journal of surfaces and colloids 20:9526-9534. Tsai MH, Wu CH, Chen WP, Shieh JY, Wen CY (2003) Subcellular distributions of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the subcommissural organ of the golden hamster (Mesocricetus auratus). Neuroscience research 47:85-95. Tsai YJ, Lin CT, Lue JH (2007) Characterization of the induced neuropeptide Y-like immunoreactivity in primary sensory neurons following complete median nerve transection. Journal of neurotrauma 24:1878-1888. Tsai YJ, Lin CT, Huang CT, Wang HY, Tien LT, Chen SH, Lue JH (2009) Neuropeptide Y modulates c-Fos protein expression in the cuneate nucleus and contributes to mechanical hypersensitivity following rat median nerve injury. Journal of neurotrauma 26:1609-1621. Wakisaka S, Kajander KC, Bennett GJ (1991) Increased neuropeptide Y (NPY)-like immunoreactivity in rat sensory neurons following peripheral axotomy. Neuroscience letters 124:200-203. Walther D, Takemura M, Uhl G (1993) Fos family member changes in nucleus caudalis neurons after primary afferent stimulation: enhancement of fos B and c-fos. Brain research Molecular brain research 17:155-159. Waxman SG, Kocsis JD, Nitta KC (1979) Lysophosphatidyl choline-induced focal demyelination in the rabbit corpus callosum. Light-microscopic observations. J Neurol Sci 44:45-53. Wiesenfeld-Hallin Z, Xu XJ, Langel U, Bedecs K, Hokfelt T, Bartfai T (1992) Galanin-mediated control of pain: enhanced role after nerve injury. Proceedings of the National Academy of Sciences of the United States of America 89:3334-3337. Woolf CJ, Fitzgerald M (1982) Do opioid peptides mediate a presynaptic control of C-fibre transmission in the rat spinal cord? Neuroscience letters 29:67-72. Yeh JH, Lue JH, Wang HY, Huang CT, Tsai YJ (2008) Synaptic relationships between induced neuropeptide Y-like immunoreactive terminals and cuneothalamic projection neurons in the rat cuneate nucleus following median nerve transection. Journal of chemical neuroanatomy 36:27-32. Yoneyama KA, Tanaka AK, Silveira TG, Takahashi HK, Straus AH (2006) Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity. Journal of lipid research 47:2171-2178.Allt G, Ghabriel MN, Sikri K (1988) Lysophosphatidyl choline-induced demyelination. A freeze-fracture study. Acta neuropathologica 75:456-464. Zhang X, Xu ZO, Shi TJ, Landry M, Holmberg K, Ju G, Tong YG, Bao L, Cheng XP, Wiesenfeld-Hallin Z, Lozano A, Dostrovsky J, Hokfelt T (1998) Regulation of expression of galanin and galanin receptors in dorsal root ganglia and spinal cord after axotomy and inflammation. Annals of the New York Academy of Sciences 863:402-413. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60648 | - |
| dc.description.abstract | 先前的研究發現，磷脂醯膽鹼會造成神經髓鞘剝離退化，並引發感覺疼痛異常，如：觸覺性疼痛和痛覺過敏等現象。本研究藉由磷脂醯膽鹼藥物處理，誘發正中神經局部去髓鞘化，再利用免疫細胞化學分析、動物疼痛行為測試，進一步探討甘丙肽類型2受器在神經病變疼痛中所擔任的機制。首先證實正常大白鼠的第六頸髓段背根神經節表現甘丙肽類型2受器免疫反應神經元為小型神經元，於正中神經4%磷脂醯膽鹼處理一週，背根神經節中甘丙肽類型2受器免疫反應神經元百分比有明顯地增加。進一步以雙重螢光免疫標誌法分析第六頸髓段背根神經節內甘丙肽類型2受器免疫反應神經元是否有神經絲蛋白200, 轉錄活化因子3, 生長相關因子43, 甘丙肽, 神經胜肽Y, 物質P, 神經性一氧化氮合成酶, 辣椒素受器1。結果顯示同時與神經絲蛋白200, 轉錄活化因子3及神經胜肽Y共存的百分比有顯著地增加；但與物質P共同表現的百分比卻是降低。結果顯示磷脂醯膽鹼處理後，誘導甘丙肽類型2受器免疫反應神經元增升部分為大型受傷的神經元且同時具有神經胜肽Y的表現。另外，動物行為測試分析正中神經施以4%磷脂醯膽鹼處理，在動物同側前肢呈現觸覺痛及溫熱覺過敏現象；當其同側前肢掌面皮下注射甘丙肽類型2受器拮抗劑M871或增效劑AR-M1896處理時，分析其行為反應及楔狀神經核內原致癌基因免疫反應神經元的數量變化。結果發現觸覺痛及楔狀神經核內原致癌基因免疫反應神經元數量以M871處理有顯著地緩解並數量下降，而施以AR-M1896有加劇且數量增加。綜合以上結果，推測磷脂醯膽鹼處理誘導甘丙肽類型2受器免疫反應神經元的增升可能透過神經胜肽Y的釋放，而促使楔狀神經核內原致癌基因免疫反應神經元的數量增加，而與觸覺痛訊息的傳遞有所關聯。 | zh_TW |
| dc.description.abstract | Previous studies have revealed that lysophosphatidylcholine could induce nerve demyelination and initiate neuropathic pain, such as allodynia and hyperalgesia. This study used lysophosphatidylcholine inducing the median nerve local demyelination, and then we investigated the role of galanin receptor 2 (GalR2) in neuropathic pain by immunohistochemistry, drug treatment and animal behavioral test. First, we found galanin receptor 2- like immunoreactive (GalR2-IL) production localized in the small-sized C6 dorsal root ganglion neurons of the normal rats, but the amount of GalR2-IL neurons peaked at one week after lysophosphatidylcholine treatment. Furthermore, we used double immunofluorescence of GalR2-IL neurons weather contained NF200, ATF-3, GAP43, Galanin, NPY, SP, nNOS, VR1. The results showed that the percentage of GalR2-IL neurons also labeled NF200, ATF3, NPY were increasing, but decreased in for SP. These results suggested that lysophosphatidylcholine treatment up-regulated GalR2 expression in A-type and injury DRG neurons further containing NPY. After 4% lysophosphatidylcholine treatment in the median nerve, animals developed tactile allodynia and thermal hyperalgesia in treated side of forelimb. Moreover, we employed lysophosphatidylcholine treatment along with GalR2 agonist (AR-M1896) or antagonist (M871) intraplantar application to examine the effect on median neuropathic pain and c-Fos expression in the stimulated side in cuneate nucleus (CN). The mechanical allodynia level and the number of c-Fos-IL neurons in the M871 group were dramatically attenuated, whereas those in the AR-M1896 group had increased compared to the saline group. These results indicated that activation lysophosphatidylcholine -upregulated GalR2-IL neurons may be possibly via promoting NPY release to evoke c-Fos expression in the CN and to transmit LPC-induced tactile hypersensitivity. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:24:39Z (GMT). No. of bitstreams: 1 ntu-102-R00446006-1.pdf: 1795776 bytes, checksum: e15b8745a4701493930c551d982ab0f2 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 致謝 I
論文口試委員議定書 II 中文摘要 1 英文摘要 2 目錄 3 壹、緒論 5 貳、實驗材料和方法 11 1.建立正中神經傷害模式 11 2.灌流動物 11 3.組織製備 12 3.1正中神經 12 3.2第六頸髓段背根神經節 12 3.2.1石蠟包埋處理 12 3.2.2組織貼片處理 13 3.2.3蠟片脫蠟水合處理 13 3.2.4免疫組織化學染色處理 13 3.2.5免疫螢光雙重標誌處理 15 4.藥物處理及動物行為測試 16 4.1動物機械性痛覺敏感刺激測試 17 4.2動物溫熱覺刺激測試 18 5.藥物處理配合電刺激實驗 18 5.1免疫細胞化學法標誌c-Fos免疫反應神經元於楔狀神經核區 19 叁、實驗結果 21 1.GalR2-IL免疫反應神經元在C6 DRG的分布變化 21 2.LPC誘導正中神經去髓鞘的情形 21 3.GalR2與疼痛相關因子在C6 DRG中共同分布比例 21 3.1 GalR2與NF200在C6 DRG中分布情形 21 3.2. GalR2與ATF-3在C6 DRG中分布情形 22 3.3 GalR2與GAP43在C6 DRG中分布情形 22 3.4 GalR2與Galanin在C6 DRG中分布情形 22 3.5 GalR2與NPY在C6 DRG中分布情形 23 3.6 GalR2與SP在C6 DRG中分布情形 23 3.7 GalR2與nNOS在C6 DRG中分布情形 24 3.8 GalR2與VR1在C6 DRG中分布情形 24 4.GalR2免疫反應神經元與在楔狀神經核分布與表現量 24 5.動物對溫熱覺刺激行為試驗 25 6.動物對機械性刺激行為試驗 25 7.術後第七天，掌面注射藥物再施以電刺激，檢測楔狀神經核內的c-Fos免疫反應神經元數量 26 肆、討論 27 伍、附圖 31 陸、參考資料 45 | |
| dc.language.iso | zh-TW | |
| dc.title | 正中神經以磷脂醯膽鹼處理活化甘丙肽類型2受器
在機械性觸覺痛扮演的角色 | zh_TW |
| dc.title | Activating Galanin Receptor 2 Plays the role in
Mechanical Allodynia Following Lysophosphatidylcholine Treatment of the Median Nerve | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡怡汝(Yi-Ju Tsai),溫振源(Chen-Yuan Wen),馮琮涵(Tsorng-Harn Fong),吳慶祥(Chingh-Siang) | |
| dc.subject.keyword | 磷脂醯膽鹼,甘丙肽,類型2受器,正中神經, | zh_TW |
| dc.subject.keyword | lysophosphatidylcholine,allodynia,Median nerve, | en |
| dc.relation.page | 51 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-16 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 解剖學暨細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 解剖學暨細胞生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-102-1.pdf 目前未授權公開取用 | 1.75 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。