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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60342完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 徐善慧(Shan-hui Hsu) | |
| dc.contributor.author | Cheng-Yen Lin | en |
| dc.contributor.author | 林政諺 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:15:56Z | - |
| dc.date.available | 2018-08-20 | |
| dc.date.copyright | 2013-08-20 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-19 | |
| dc.identifier.citation | 1. Puskas, J. E., & Chen, Y. (2004). Biomedical application of commercial polymers and novel polyisobutylene-based thermoplastic elastomers for soft tissue replacement. Biomacromolecules, 5(4), 1141-1154.
2. 山西省化工研究所.聚氨酯彈性體手冊.化學工業出版社. 2000. 3. Hepburn, C. (1982). Polyurethane elastomers (p. 50). London: Applied Science Publishers. 4. Demir, M. M., Yilgor, I., Yilgor, E. E. A., & Erman, B. (2002). Electrospinning of polyurethane fibers. Polymer, 43(11), 3303-3309. 5. Jabbari, E., & Khakpour, M. (2000). Morphology of and release behavior from porous polyurethane microspheres. Biomaterials, 21(20), 2073-2079. 6. Jain, P., & Pradeep, T. (2005). Potential of silver nanoparticle‐coated polyurethane foam as an antibacterial water filter. Biotechnology and bioengineering, 90(1), 59-63. 7. Noble, K. L. (1997). Waterborne polyurethanes. Progress in organic coatings, 32(1), 131-136. 8. Lee, S. Y., Lee, J. S., & Kim, B. K. (1997). Preparation and Properties of Water‐borne Polyurethanes. Polymer International, 42(1), 67-76. 9. Rahman, M. M., & Kim, H. D. (2006). Synthesis and characterization of waterborne polyurethane adhesives containing different amount of ionic groups (I). Journal of applied polymer science, 102(6), 5684-5691. 10. Zhang, J., Zhang, X. Y., Dai, J. B., & Li, W. H. (2010). Synthesis and characterization of yellow water-borne polyurethane using a diol colorant as extender. Chinese Chemical Letters, 21(2), 143-145. 11. Cakić, S. M., Ristić, I. S., Cincović, M. M., & Špirkova, M. (2012). The effects of the structure and molecular weight of the macrodiol on the properties polyurethane anionic adhesives. International Journal of Adhesion and Adhesives. 12. Chen, H., Fan, Q., Chen, D., & Yu, X. (2001). Synthesis and properties of polyurethane modified with an aminoethylaminopropyl‐substituted polydimethylsiloxane. II. Waterborne polyurethanes. Journal of applied polymer science, 79(2), 295-301. 13. 顏明雄,蔡坪芫.水性PU 混成物應用於織物整理加工. 2002. 14. Gopferich, A. (1996). Mechanisms of polymer degradation and erosion.Biomaterials, 17(2), 103-114. 15. 王建.生物可降解高分子及其應用[J].四川紡織科技,2003(3):14-17. 16. 梁飛,楊穎,餘蕾,賈敏. 可降解水性聚氨酯的合成及其性能研究.塗料工程. 2012. 9(4): 53-55. 17. Yu, L., Zhou, L., Ding, M., Li, J., Tan, H., Fu, Q., & He, X. (2011). Synthesis and characterization of novel biodegradable folate conjugated polyurethanes.Journal of colloid and interface science, 358(2), 376-383. 18. Sivak, W. N., Zhang, J., Petoud, S., & Beckman, E. J. (2010). Incorporation of ionic ligands accelerates drug release from LDI–glycerol polyurethanes. Acta Biomaterialia, 6(1), 144-153. 19. Al‐Salah, H. A., Xiao, H. X., McLean Jr, J. A., & Frisch, K. C. (1988). Polyurethane cationomers. I. Structure–properties relationships. Journal of Polymer Science Part A: Polymer Chemistry, 26(6), 1609-1620. 20. Chen SA, Chan WC. Polyurethane cationmers, I, Structure Property relationship. Journal of Polymer Science Part B: Polymer Physics. 1990. 20: 1499-1514 21. Lee JS, Kim BK. Polyurethane cationomers from poly(propylene) glycol and isophorone diisocyanate: emulsion characteristics and tensile properties of cast films. Progress in Organic Coatings. 1995. 25: 311-318. 22. 趙雨花 ,亢茂青, 王心葵. 高性能水性聚氨酯黏膠劑[J]. 化工新型材料. 2005. 33(9): 73-75. 23. 項尚林, 陳瑞珠, 李瑩, 韓徐. 內交聯型複合薄膜用水性聚氨酯黏膠劑的研製[J]. 包裝工程. 2006. 27(2): 39-42. 24. 王萃萃,張彪,劉瀏,許戈文. 水性聚氨酯基生物可降解材料製備方法與應用. 聚氨酯,2009.12: 60-64. 25. 王仕財, 朱春鳳, 毛建衛.生物可降解植物基聚氨酯材料研究進展[J].浙江科技學院學.2008.20(3) : 184-188. 26. 戈進傑.生物降解高分子材料及其應用[M].北京:化學工業出版社.2003. 27. Santerre, J. P., Woodhouse, K., Laroche, G., & Labow, R. S. (2005). Understanding the biodegradation of polyurethanes: from classical implants to tissue engineering materials. Biomaterials, 26(35), 7457-7470. 28. Timmins M, Liebmann-Vinson A. Biodegradable polymers: degradation mechanisms, Part 1. PBM series .2003. 9: 2. 29. Guelcher, S. A., Gallagher, K. M., Didier, J. E., Klinedinst, D. B., Doctor, J. S., Goldstein, A. S., ... & Hollinger, J. O. (2005). Synthesis of biocompatible segmented polyurethanes from aliphatic diisocyanates and diurea diol chain extenders. Acta Biomaterialia, 1(4), 471-484. 30. Younes, H. M., Bravo-Grimaldo, E., & Amsden, B. G. (2004). Synthesis, characterization and in vitro degradation of a biodegradable elastomer.Biomaterials, 25(22), 5261-5269. 31. Sheth, M., Kumar, R. A., Dave, V., Gross, R. A., & McCarthy, S. P. (1997). Biodegradable polymer blends of poly (lactic acid) and poly (ethylene glycol).Journal of Applied Polymer Science, 66(8), 1495-1505. 32. Loh, X. J., Tan, K. K., Li, X., & Li, J. (2006). The in vitro hydrolysis of poly (ester urethane) s consisting of poly [(R)-3-hydroxybutyrate] and poly (ethylene glycol). Biomaterials, 27(9), 1841-1850. 33. Hassan, M. K., Mauritz, K. A., Storey, R. F., & Wiggins, J. S. (2006). Biodegradable aliphatic thermoplastic polyurethane based on poly (ε‐caprolactone) and L‐lysine diisocyanate. Journal of Polymer Science Part A: Polymer Chemistry, 44(9), 2990-3000. 34. Gorna, K., & Gogolewski, S. (2002). Biodegradable polyurethanes for implants. II. In vitro degradation and calcification of materials from poly (ϵ‐caprolactone)–poly (ethylene oxide) diols and various chain extenders. Journal of biomedical materials research, 60(4), 592-606. 35. Hiltunen, K., Tuominen, J., & Seppala, J. V. (1998). Hydrolysis of lactic acid based poly (ester‐urethane) s. Polymer international, 47(2), 186-192. 36. Lendlein, A., Colussi, M., Neuenschwander, P., & Suter, U. W. (2001). Hydrolytic degradation of phase‐segregated multiblock copoly (ester urethane) s containing weak links. Macromolecular Chemistry and Physics, 202(13), 2702-2711. 37. Deschamps, A. A., Van Apeldoorn, A. A., Hayen, H., De Bruijn, J. D., Karst, U., Grijpma, D. W., & Feijen, J. (2004). In vivo and in vitro degradation of poly (ether ester) block copolymers based on poly (ethylene glycol) and poly (butylene terephthalate). Biomaterials, 25(2), 247-258. 38. Mohanty, A. K., Misra, M., & Hinrichsen, G. (2000). Biofibres, biodegradable polymers and biocomposites: an overview. Macromolecular Materials and Engineering, 276(1), 1-24. 39. Gogolewski, S., & Pennings, A. J. (1983). An artificial skin based on biodegradable mixtures of polylactides and polyurethanes for full‐thickness skin wound covering. Die Makromolekulare Chemie, Rapid Communications, 4(10), 675-680. 40. Gogolewski, S., & Gorna, K. (2007). Biodegradable polyurethane cancellous bone graft substitutes in the treatment of iliac crest defects. Journal of Biomedical Materials Research Part A, 80(1), 94-101. 41. Sivak, W. N., Pollack, I. F., Petoud, S., Zamboni, W. C., Zhang, J., & Beckman, E. J. (2008). Catalyst-dependent drug loading of LDI–glycerol polyurethane foams leads to differing controlled release profiles. Acta Biomaterialia, 4(5), 1263-1274. 42. Zhang, J., Wu, M., Yang, J., Wu, Q., & Jin, Z. (2009). Anionic poly (lactic acid)-polyurethane micelles as potential biodegradable drug delivery carriers. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 337(1), 200-204. 43. Gogolewski, S., Gorna, K., Zaczynska, E., & Czarny, A. (2008). Structure–property relations and cytotoxicity of isosorbide‐based biodegradable polyurethane scaffolds for tissue repair and regeneration. Journal of Biomedical Materials Research Part A, 85(2), 456-465. 44. Li, B., Yoshii, T., Hafeman, A. E., Nyman, J. S., Wenke, J. C., & Guelcher, S. A. (2009). The effects of rhBMP-2 released from biodegradable polyurethane/microsphere composite scaffolds on new bone formation in rat femora. Biomaterials, 30(35), 6768-6779. 45. Gunatillake P, Mayadunne R, Adhikari R. Recent developments in biodegradable polymers. Biotechnology Annual Review. 2006. 12: 1387-2656. 46. Holland, S. J., Tighe, B. J., & Gould, P. L. (1986). Polymers for biodegradable medical devices. 1. The potential of polyesters as controlled macromolecular release systems. Journal of Controlled Release, 4(3), 155-180. 47. Uhrich, K. E., Cannizzaro, S. M., Langer, R. S., & Shakesheff, K. M. (1999). Polymeric systems for controlled drug release. Chemical Reviews, 99(11), 3181-3198. 48. Pannier, A. K., & Shea, L. D. (2004). Controlled release systems for DNA delivery. Molecular Therapy, 10(1), 19-26. 49. Rahman, N. A., & Mathiowitz, E. (2004). Localization of bovine serum albumin in double-walled microspheres. Journal of controlled release, 94(1), 163-175. 50. Lee, E. S., Park, K. H., Kang, D., Park, I. S., Min, H. Y., Lee, D. H., ... & Na, K. (2007). Protein complexed with chondroitin sulfate in poly (lactide-co-glycolide) microspheres. Biomaterials, 28(17), 2754-2762. 51. Zhu, C., Jung, S., Luo, S., Meng, F., Zhu, X., Park, T. G., & Zhong, Z. (2010). Co-delivery of siRNA and paclitaxel into cancer cells by biodegradable cationic micelles based on PDMAEMA–PCL–PDMAEMA triblock copolymers.Biomaterials, 31(8), 2408-2416. 52. Yang, Y. Y., Chung, T. S., & Ping Ng, N. (2001). Morphology, drug distribution, and in vitro release profiles of biodegradable polymeric microspheres containing protein fabricated by double-emulsion solvent extraction/evaporation method. Biomaterials, 22(3), 231-241. 53. Bae, S. E., Son, J. S., Park, K., & Han, D. K. (2009). Fabrication of covered porous PLGA microspheres using hydrogen peroxide for controlled drug delivery and regenerative medicine. Journal of Controlled Release, 133(1), 37-43. 54. Zhao, C., Liu, X., Nomizu, M., & Nishi, N. (2004). Preparation of DNA-loaded polysulfone microspheres by liquid–liquid phase separation and its functional utilization. Journal of colloid and interface science, 275(2), 470-476. 55. Blaker, J. J., Knowles, J. C., & Day, R. M. (2008). Novel fabrication techniques to produce microspheres by thermally induced phase separation for tissue engineering and drug delivery. Acta biomaterialia, 4(2), 264-272. 56. He, P., Davis, S. S., & Illum, L. (1999). Chitosan microspheres prepared by spray drying. International Journal of Pharmaceutics, 187(1), 53-65. 57. Giunchedi, P., Conti, B., Genta, I., Conte, U., & Puglisi, G. (2001). Emulsion spray-drying for the preparation of albumin-loaded PLGA microspheres. Drug development and industrial pharmacy, 27(7), 745-750. 58. Costantino, H. R., Firouzabadian, L., Hogeland, K., Wu, C., Beganski, C., Carrasquillo, K. G., ... & Tracy, M. A. (2000). Protein spray-freeze drying. Effect of atomization conditions on particle size and stability. Pharmaceutical research, 17(11), 1374-1382. 59. Cheow, W. S., Ng, M. L. L., Kho, K., & Hadinoto, K. (2011). Spray-freeze-drying production of thermally sensitive polymeric nanoparticle aggregates for inhaled drug delivery: Effect of freeze-drying adjuvants. International journal of pharmaceutics, 404(1), 289-300. 60. Pollert, E., Knižek, K., Maryško, M., Zavěta, K., Lančok, A., Bohaček, J., ... & Babič, M. (2006). Magnetic poly (glycidyl methacrylate) microspheres containing maghemite prepared by emulsion polymerization. Journal of magnetism and magnetic materials, 306(2), 241-247. 61. Andreas, K., Zehbe, R., Kazubek, M., Grzeschik, K., Sternberg, N., Baumler, H., ... & Ringe, J. (2011). Biodegradable insulin-loaded PLGA microspheres fabricated by three different emulsification techniques: Investigation for cartilage tissue engineering. Acta biomaterialia, 7(4), 1485-1495. 62. Yeum, J. H., Sun, Q., & Deng, Y. (2005). Poly (vinyl acetate)/silver nanocomposite microspheres prepared by suspension polymerization at low temperature. Macromolecular materials and engineering, 290(1), 78-84. 63. Cho, J. S., Kwon, A., & Cho, C. G. (2002). Microencapsulation of octadecane as a phase-change material by interfacial polymerization in an emulsion system. Colloid and polymer science, 280(3), 260-266. 64. Eggerstedt, S. N., Dietzel, M., Sommerfeld, M., Suverkrup, R., & Lamprecht, A. (2012). Protein spheres prepared by drop jet freeze drying. International Journal of Pharmaceutics. 65. Harland, R. S., Dubernet, C., Benoit, J. P., & Peppas, N. A. (1988). A model of dissolution-controlled, diffusional drug release from non-swellable polymeric microspheres. Journal of controlled release, 7(3), 207-215. 66. O'hagan, D. T., Rahman, D., McGee, J. P., Jeffery, H., Davies, M. C., Williams, P., ... & Challacombe, S. J. (1991). Biodegradable microparticles as controlled release antigen delivery systems. Immunology, 73(2), 239. 67. Bae, S. E., Son, J. S., Park, K., & Han, D. K. (2009). Fabrication of covered porous PLGA microspheres using hydrogen peroxide for controlled drug delivery and regenerative medicine. Journal of Controlled Release, 133(1), 37-43. 68. Anderson, J. M., & Shive, M. S. (1997). Biodegradation and biocompatibility of PLA and PLGA microspheres. Advanced drug delivery reviews, 28(1), 5-24. 69. Reuveny, S., & Thoma, R. W. (1986). Apporotus and Methodology for Microcorrier Cell Culture. Advances in applied microbiology, 31, 139. 70. Van Wezel, A. L. (1967). Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature, 216, 64-65. 71. Malda, J., & Frondoza, C. G. (2006). Microcarriers in the engineering of cartilage and bone. Trends in biotechnology, 24(7), 299-304. 72. Keese, C. R., & Giaever, I. (1983). Cell growth on liquid microcarriers. Science,219(4591), 1448-1449. 73. Tao, X., Shaolin, L., & Yaoting, Y. (2003). Preparation and culture of hepatocyte on gelatin microcarriers. Journal of Biomedical Materials Research Part A,65(2), 306-310. 74. Yang, Y., Rossi, F., & Putnins, E. E. (2007). < i> Ex vivo</i> expansion of rat bone marrow mesenchymal stromal cells on microcarrier beads in spin culture. Biomaterials, 28(20), 3110-3120. 75. Akins, R. E., Boyce, R. A., Madonna, M. L., Schroedl, N. A., Gonda, S. R., McLaughlin, T. A., & Hartzell, C. R. (1999). Cardiac organogenesis in vitro: reestablishment of three-dimensional tissue architecture by dissociated neonatal rat ventricular cells. Tissue engineering, 5(2), 103-118. 76. Piskin, E., Kiremitci, M., Denkbas, E. B., Gurhan, I., Gombotz, W. R., & Hoffmanc, A. S. (1992). Cell culturing on polymeric beads. Clinical materials, 11(1), 171-178. 77. Chen, X. G., Liu, C. S., Liu, C. G., Meng, X. H., Lee, C. M., & Park, H. J. (2006). Preparation and biocompatibility of chitosan microcarriers as biomaterial. Biochemical engineering journal, 27(3), 269-274. 78. Wu, C., Pan, J., Bao, Z., & Yu, Y. (2007). Fabrication and characterization of chitosan microcarrier for hepatocyte culture. Journal of Materials Science: Materials in Medicine, 18(11), 2211-2214. 79. Matsushita, T., Ketayama, M., Kamihata, K. I., & Funatsu, K. (1990). Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment. Applied microbiology and biotechnology,33(3), 287-290. 80. Sato, Y., Ochiya, T., Yasuda, Y., & Matsubara, K. (1994). A new three‐dimensional culture system for hepatocytes using reticulated polyurethane. Hepatology, 19(4), 1023-1028. 81. Varani, J., Bendelow, M. J., Chun, J. H., & Hillegas, W. A. (1986). Cell growth on microcarriers: comparison of proliferation on and recovery from various substrates. Journal of biological standardization, 14(4), 331-336. 82. Frauenschuh, S., Reichmann, E., Ibold, Y., Goetz, P. M., Sittinger, M., & Ringe, J. (2007). A Microcarrier‐Based Cultivation System for Expansion of Primary Mesenchymal Stem Cells. Biotechnology progress, 23(1), 187-193. 83. Abranches, E., Bekman, E., Henrique, D., & Cabral, J. (2007). Expansion of mouse embryonic stem cells on microcarriers. Biotechnology and bioengineering, 96(6), 1211-1221. 84. Lock, L. T., & Tzanakakis, E. S. (2009). Expansion and differentiation of human embryonic stem cells to endoderm progeny in a microcarrier stirred-suspension culture. Tissue Engineering Part A, 15(8), 2051-2063. 85. Jacobson, B. S., & Ryan, U. S. (1982). Growth of endothelial and HeLa cells on a new multipurpose microcarrier that is positive, negative or collagen coated. Tissue and cell, 14(1), 69-83. 86. Chun, K. W., Yoo, H. S., Yoon, J. J., & Park, T. G. (2004). Biodegradable PLGA microcarriers for injectable delivery of chondrocytes: effect of surface modification on cell attachment and function. Biotechnology progress, 20(6), 1797-1801. 87. Chen, R., Curran, S. J., Curran, J. M., & Hunt, J. A. (2006). The use of poly (l-lactide) and RGD modified microspheres as cell carriers in a flow intermittency bioreactor for tissue engineering cartilage. Biomaterials, 27(25), 4453-4460. 88. Kim, H. W., Gu, H. J., & Lee, H. H. (2007). Microspheres of collagen-apatite nanocomposites with osteogenic potential for tissue engineering. Tissue engineering, 13(5), 965-973. 89. Kim, B. K., & Lee, J. C. (1996). Waterborne polyurethanes and their properties. Journal of Polymer Science Part A: Polymer Chemistry, 34(6), 1095-1104. 90. Han CD. Multi-phase flow in polymer processing. Academic Press, New York. 1981. 91. Tamada, Y., & Ikada, Y. (1994). Fibroblast growth on polymer surfaces and biosynthesis of collagen. Journal of biomedical materials research, 28(7), 783-789. 92. Mei, N., Zhou, P., Pan, L. F., Chen, G., Wu, C. G., Chen, X., ... & Chen, G. Q. (2006). Biocompatibility of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) modified by silk fibroin. Journal of Materials Science: Materials in Medicine, 17(8), 749-758. 93. Bonart R, Hoffmann K. Orientational behavior of segmented polyether urethanes as a function of strain and solvents used. Macromolecular Chemistry and Physics. 1983. 184(7): 1529-1546. 94. Lee, Y. M., Lee, J. C., & Kim, B. K. (1994). Effect of soft segment length on the properties of polyurethane anionomer dispersion. Polymer, 35(5), 1095-1099. 95. Kim, B. K., Lee, S. Y., & Xu, M. (1996). Polyurethanes having shape memory effects. Polymer, 37(26), 5781-5793. 96. Kondo, M., Niwa, T., Okamoto, H., & Danjo, K. (2009). Particle characterization of poorly water-soluble drugs using a spray freeze drying technique. Chemical and Pharmaceutical Bulletin, 57(7), 657-662. 97. Hindmarsh, J. P., Russell, A. B., & Chen, X. D. (2007). Fundamentals of the spray freezing of foods—microstructure of frozen droplets. Journal of food engineering, 78(1), 136-150. 98. Cheow, W. S., Ng, M. L. L., Kho, K., & Hadinoto, K. (2011). Spray-freeze-drying production of thermally sensitive polymeric nanoparticle aggregates for inhaled drug delivery: Effect of freeze-drying adjuvants. International journal of pharmaceutics, 404(1), 289-300. 99. Zhang, H., Hussain, I., Brust, M., Butler, M. F., Rannard, S. P., & Cooper, A. I. (2005). Aligned two-and three-dimensional structures by directional freezing of polymers and nanoparticles. Nature materials, 4(10), 787-793. 100. Zhang, H., Edgar, D., Murray, P., Rak‐Raszewska, A., Glennon‐Alty, L., & Cooper, A. I. (2008). Synthesis of porous microparticles with aligned porosity. Advanced Functional Materials, 18(2), 222-228. 101. SHIRAI, Y., NAKANISHI, K., MATSUNO, R., & KAMIKUBO, T. (1985). Effects of polymers on secondary nucleation of ice crystals. Journal of Food Science, 50(2), 401-406. 102. Burke, P. A., Klumb, L. A., Herberger, J. D., Nguyen, X. C., Harrell, R. A., & Zordich, M. (2004). Poly (lactide-co-glycolide) microsphere formulations of darbepoetin alfa: spray drying is an alternative to encapsulation by spray-freeze drying.Pharmaceutical research,21(3), 500-506. 103. Loh, X. J., Colin Sng, K. B., & Li, J. (2008). Synthesis and water-swelling of thermo-responsive poly (ester urethane) s containing poly (ε-caprolactone), poly (ethylene glycol) and poly (propylene glycol). Biomaterials, 29(22), 3185-3194. 104. Ahmadi, R., Mordan, N., Forbes, A., & Day, R. M. (2011). Enhanced attachment, growth and migration of smooth muscle cells on microcarriers produced using thermally induced phase separation. Acta Biomaterialia, 7(4), 1542-1549. 105. Chun, K. W., Yoo, H. S., Yoon, J. J., & Park, T. G. (2004). Biodegradable PLGA microcarriers for injectable delivery of chondrocytes: effect of surface modification on cell attachment and function. Biotechnology progress, 20(6), 1797-1801. 106. Jabbarzadeh, E., Jiang, T., Deng, M., Nair, L. S., Khan, Y. M., & Laurencin, C. T. (2007). Human endothelial cell growth and phenotypic expression on three dimensional poly (lactide‐co‐glycolide) sintered microsphere scaffolds for bone tissue engineering. Biotechnology and bioengineering, 98(5), 1094-1102. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60342 | - |
| dc.description.abstract | 本研究藉由改變水性聚胺酯彈性體之軟鏈段種類，合成出兩種具有生物可降解性之水性聚胺酯 (waterborne biodegradable polyurethane, WBDPU)乳液，並利用噴霧冷凍乾燥法將其奈米顆粒自組裝形成微球，以雷射光散射方式量測微球之粒徑，其尺寸大小約在50 ~ 60 um，以高固含量情形下加工可獲得具有緻密皮層之微球，而以乳液低固含量情形下依聚胺酯軟鏈段的改變而可獲得不同的表面型態，說明藉由簡單調控聚胺酯軟鏈段種類及乳液固含量可製備出具有不同表面型態之微球。由體外測試結果得知，以PEB diol (Mn=2000 g/mol) 搭配 PCL diol (Mn=2000 g/mol) 作為軟鏈段 (PEB diol:PCL diol=60 wt%：40 wt%) 所合成出的水性聚胺酯 (PU02)與以純PCL diol (Mn=2000 g/mol) 作為軟鏈段所合成出的水性聚胺酯 (PU01) 相比，降解速率較快。以亞甲基藍做為親水性模擬藥物，添加於水性聚胺酯乳液加工製備出載藥微球，測試其藥物釋放，以PU02乳液固含量為10 wt%所製備出的載藥微球，在6 小時內的藥物突釋量最高，而以PU01乳液固含量為30 wt%所製備出的載藥微球在相同測試時間內之藥物突釋量最低。以L929纖維母細胞測試水性聚胺酯微球體之生物相容性，發現共培養24 h 後纖維母細胞可貼附於微球表面並增生，隨其表面型態的不同，而有不同的貼附程度。此外，我們發現水性生物可降解聚胺酯微球體具有自組裝能力，可進一步自組裝成薄膜及三維支架，自組裝薄膜的機械性質與乳液塗佈所獲得之薄膜相似，以低分子量正電荷的幾丁聚醣進行微球表面改質，可改變聚胺酯微球之自組裝行為。自組裝三維支架可藉調控微球分散液的濃度來控制支架之孔洞，同時可成功將纖維母細胞植入自組裝三維支架之中。因此本研究之水性聚胺酯微球具有作為藥物釋放及細胞載體之潛力，同時可進一步應用於組織工程支架。 | zh_TW |
| dc.description.abstract | Two types of waterborne biodegradable polyurethane (WBDPU) in the form of homogeneous nanoparticles (NPs) were synthesized using biodegradable polyesters as soft segment. The first WBDPU (PU01) was based on polycaprolactone diol (PCL diol, Mw 2000) and the second WBDPU (PU02) was based on 40% PCL diol and 60% polyethylene butylene adipate diol (PEB diol, Mw 2000). The dispersion of WBDPU NPs were prepared in different solid contents, sprayed into liquid nitrogen, and resuspended in water. During the process, the NPs were self-assembled into microspheres, with an average size of 50-60 um. By adjusting the contents of NPs, microspheres could be obtained with different porosity. In vitro degradation results revealed that microspheres from PU02 (i.e. PU02 MS) has faster degradation rate than those from PU01 (PU01 MS). The release of methylene blue encapsulated during MS formation was investigated. PU02 MS made from 10% dispersion (i.e. PU02 MS_10) showed a greater burst release at 6 hours, whereas PU01 MS_30 had significantly lower burst release. Biocompatibility evaluation using L929 fibroblasts demonstrated that cells could attach on the microspheres after 24 hours. On the other hand, the microspheres may self-assemble further into films and scaffolds. The mechanical properties of self-assembled solid films from microspheres were similar to those from NP dispersion. Surface modification of microspheres by low molecular weight positively charged chitosan may modify the self-assembly behavior of microspheres. Scaffolds made of microspheres may have different porous structure by controlling the amount of microspheres that built up the scaffolds. Fibroblasts were successfully seeded and grown in the scaffolds. We concluded that the biodegradable and elastic microspheres with potential applications in drug release and cell carriers may be facilely produced from a green and sustainable process. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:15:56Z (GMT). No. of bitstreams: 1 ntu-102-R00549021-1.pdf: 12322568 bytes, checksum: f01660380dd48eb1f38e013d70c863eb (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 目錄
致謝……………………………………………………………………………………I 摘要…………………………………………………………………………………II Abstract…………………………………………………………………III 目錄……………………………………………………………………………………V 圖目錄……………………………………………………………………………IX 表目錄……………………………………………………………………………XI 第一章 文獻回顧……………………………………………………………1 1-1. 彈性體…………………………………………………………………1 1-1-1. 聚胺酯……………………………………………………………2 1-1-2. 生物可降解高分子………………………………………3 1-1-3. 水性生物可降解聚胺酯(WBDPU)……………3 1-2. 藥物釋放………………………………………………………………5 1-3. 細胞微載體……………………………………………………………7 1-4. 研究目的…………………………………………………………………8 第二章 研究方法…………………………………………………………………9 2-1. 研究架構……………………………………………………………………9 2-2. 水性生物可降解聚胺酯(Waterborne biodegradable polyurethane, WBDPU)……………………………………………………9 2-2-1. 水性生物可降解聚胺酯合成……………………………………9 2-2-2. 水性生物可降解聚胺酯微球體之製備…………………15 2-3. 水性生物可降解聚胺酯之物化特性分析……………………16 2-3-1. 粒徑與界面電位分析………………………………………………16 2-3-2. 接觸角分析…………………………………………………………………16 2-3-3. 衰減全反射傅立葉紅外光譜儀………………………………16 2-3-4. 熱性質分析……………………………………………………………………16 2-3-5. 膨潤率測試……………………………………………………………………16 2-3-6. 拉伸試驗…………………………………………………………………………17 2-3-7. 核磁共振儀……………………………………………………………………17 2-4. 水性生物可降解聚胺酯微球體之物化性分析………………17 2-4-1. 微球體粒徑及尺寸分佈量測………………………………………18 2-4-2. 掃描式電子顯微鏡分析………………………………………………18 2-4-3. 體外降解速率測試 產物分析……………………………………18 2-4-4. 藥物釋放…………………………………………………………………………19 2-5. 水性生物可降解聚胺酯微球體之生物相容性分析………19 2-5-1. 小鼠皮膚纖維母細胞貼附與增生測試………………………19 2-6. 微球自組裝……………………………………………………………………………21 2-6-1. 自組裝薄膜製備………………………………………………………………21 2-6-2. 表面改質………………………………………………………………………………21 2-6-3. 自組裝支架製備………………………………………………………………21 2-7. 自組裝薄膜及支架之物性分析…………………………………………22 2-7-1. 拉伸試驗……………………………………………………………………………22 2-7-2. 掃描式電子顯微鏡分析…………………………………………………22 2-7-3. 孔隙率與吸水率分析………………………………………………………22 2-8. 支架之生物相容性分析…………………………………………………………23 第三章 實驗結果…………………………………………………………………………………24 3-1. 水性生物可降解聚胺酯的合成………………………………………………24 3-2. 水性生物可降解聚胺酯之薄膜物化分析…………………………24 3-2-1. 表面紅外光圖譜分析………………………………………………………24 3-2-2. 熱性質分析…………………………………………………………………………25 3-2-3. 拉伸測試及膨潤率分析……………………………………………………25 3-2-4. 核磁共振光譜………………………………………………………………………26 3-3. 水性生物可降解聚胺酯微球體之物化性分析……………………26 3-3-1. 微球體尺寸分佈…………………………………………………………………26 3-3-2. 掃描式電子顯微鏡分析……………………………………………………27 3-3-3. 體外降解速率測試 產物分析…………………………………………27 3-3-4. 藥物釋放………………………………………………………………………………28 3-4.水性生物可降解聚胺酯微球體之生物相容性分析……………28 3-4-1. 小鼠皮膚纖維母細胞貼附與增生測試…………………………28 3-5. 微球體自組裝……………………………………………………………………………29 3-5-1. 自組裝薄膜……………………………………………………………………………29 3-5-2. 表面改質………………………………………………………………………………29 3-5-3. 拉伸測試………………………………………………………………………………29 3-5-4. 自組裝支架……………………………………………………………………………30 3-5-5. 孔隙率與吸水率分析……………………………………………………………30 3-5-6. 小鼠皮膚纖維母細胞植覆率測試……………………………………31 第四章 討論……………………………………………………………………………………………32 4-1. 水性生物可降解聚胺酯之物化特性分析………………………………32 4-1-1. 粒徑與界面電位分析……………………………………………………………32 4-1-2. 接觸角分析……………………………………………………………………………32 4-1-3. 拉伸試驗及膨潤率測試………………………………………………………32 4-1-4. 衰減全反射傅立葉紅外光譜儀……………………………………………33 4-1-5. 熱性質分析………………………………………………………………………………33 4-1-6. 核磁共振光譜分析…………………………………………………………………34 4-2. 水性生物可降解聚胺酯微球體之物性、化性分析………………34 4-2-1. 微球體粒徑分析及尺寸分佈…………………………………………………34 4-2-2. 掃描式電子顯微鏡分析…………………………………………………………35 4-2-3. 體外降解速率測試 產物分析………………………………………………36 4-2-4. 藥物釋放……………………………………………………………………………………36 4-3.水性生物可降解聚胺酯微球體之生物相容性分析……………………37 4-3-1. 小鼠皮膚纖維母細胞貼附與增生測試…………………………………37 4-4. 微球體自組裝行為…………………………………………………………………………38 4-4-1. 自組裝薄膜…………………………………………………………………………………38 4-4-2. 表面改質………………………………………………………………………………………38 4-4-3. 自組裝支架…………………………………………………………………………………39 4-4-4. 小鼠皮膚纖維母細胞植覆率測試…………………………………………40 4-5 未來展望………………………………………………………………………………………………41 第五章 結論……………………………………………………………………………………………………42 參考文獻…………………………………………………………………………………………………………43 圖表…………………………………………………………………………………………………………………56 圖目錄 圖 2-1 實驗架構流程圖……………………………………………………………………………11 圖 2-2 以PCL diol作為軟鏈段合成水性可降解聚胺酯示意圖……13 圖 2-3 以PCL diol搭配PEB diol作為軟鏈段合成水性可降解聚胺酯示意圖 ……………………………………………………………………………………………………………………………………14 圖 2-4 噴霧裝置示意圖……………………………………………………………………………………15 圖 3-1水性生物可降解聚胺酯之 ATR-IR 分析圖……………………………………56 圖 3-2水性生物可降解聚胺酯之 TGA 分析圖(a)PU01 (b)PU02…………56 圖 3-3水性生物可降解聚胺酯之 DSC 分析圖(a)PU01 (b)PU02…………57 圖 3-4水性生物可降解聚胺酯之應力-應變圖(a) PU01；(b) PU02……57 圖 3-5 PU01 NMR圖譜……………………………………………………58 圖 3-6 PU02 NMR圖譜……………………………………………………59 圖 3-7 以雷射光散射方式測量微球體之粒徑及尺寸分布 (Volume distribution) (a)PU01 MS_10；(a)PU01 MS_30；(a)PU02 MS_10；(a)PU02 MS_30…………………………………………………………………………60 圖 3-8 以PCL diol作為軟鏈段所組成之水性生物可降解聚胺酯微球………………61 圖 3-9 以 PCL diol及 PEB diol作為軟鏈段 (PCL:PEB=40:60)所組成之水性生物可降解聚胺酯微球…………………………………………………………62 圖 3-10 聚胺酯微球在DDW之中以50 | |
| dc.language.iso | zh-TW | |
| dc.subject | 水性聚胺酯 | zh_TW |
| dc.subject | 生物可降解 | zh_TW |
| dc.subject | 微球 | zh_TW |
| dc.subject | 藥物釋放 | zh_TW |
| dc.subject | 細胞載體 | zh_TW |
| dc.subject | cell carrier | en |
| dc.subject | biodegradable | en |
| dc.subject | microsphere | en |
| dc.subject | drug release | en |
| dc.subject | Waterborne polyurethane | en |
| dc.title | 可降解聚胺酯微球之製備 | zh_TW |
| dc.title | Fabrication of biodegradable polyurethane microspheres | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張振榮(Chen-Jung Chang),湯正明(Cheng-Ming Tang),張瑞芝(Jui-Chih Chang),洪慧珊(Huey-Shan Hung) | |
| dc.subject.keyword | 水性聚胺酯,生物可降解,微球,藥物釋放,細胞載體, | zh_TW |
| dc.subject.keyword | Waterborne polyurethane,biodegradable,microsphere,drug release,cell carrier, | en |
| dc.relation.page | 70 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-19 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
| 顯示於系所單位： | 高分子科學與工程學研究所 | |
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