請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59799完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王永松 | |
| dc.contributor.author | Tien-Tsan Lin | en |
| dc.contributor.author | 林天讚 | zh_TW |
| dc.date.accessioned | 2021-06-16T09:38:35Z | - |
| dc.date.available | 2020-02-17 | |
| dc.date.copyright | 2017-02-17 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2017-02-09 | |
| dc.identifier.citation | Adachi, S., Ijiri, S., Kazeto, Y., & Yamaguchi, K. (2003). Oogenesis in the Japanese Eel, Anguilla japonica. In K. Aida, K. Tsukamoto, & K. Yamauchi (Eds.), Eel biology (pp. 301-317). Tokyo: Springer.
Aoyama, J., & Miller, M. J. (2003). The silver eel. In K. Aida, K. Tsukamoto, & K. Yamauchi (Eds.), Eel biology (pp. 107-117). Tokyo: Springer. Chen, S. N., & Kou, G. H. (1981). A cell line derived from Japanese eel (Anguilla japonica) ovary. Fish Pathology, 16(3), 129-137. Driancourt, M. A., Reynaud, K., Cortvrindt, R., & Smitz, J. (2000). Roles of KIT and KIT LIGAND in ovarian function. Reviews of Reproduction, 5(3), 143-152. Farini, D., La Sala, G., Tedesco, M., & De Felici, M. (2007). Chemoattractant action and molecular signaling pathways of Kit ligand on mouse primordial germ cells. Developmental Biology, 306(2), 572-583. Han, Y. S., Tzeng, W. N., Huang, Y. S., & C., L. I. (2001). Silvering in the eel: changes in morphology, body fat content and gonadal development. Journal of Taiwan Fisheres Researsh, 9, 119-127. Henkel, C. V., Dirks, R. P., de Wijze, D. L., Minegishi, Y., Aoyama, J., Jansen, H. J., Turner, B., Knudsen, B., Bundgaard, M., Hvam, K. L., Boetzer, M., Pirovano, W., Weltzien, F. A., Dufour, S., Tsukamoto, K., Spaink, H. P., & van den Thillart, G. E. E. J. M. (2012). First draft genome sequence of the Japanese eel, Anguilla japonica. Gene, 511(2), 195-201. Hultman, K. A., Bahary, N., Zon, L. I., & Johnson, S. L. (2007). Gene duplication of the zebrafish kit ligand and partitioning of melanocyte development functions to kit ligand a. Plos Genetics, 3(1). Hutt, K. J., McLaughlin, E. A., & Holland, M. K. (2006). Kit ligand and c-Kit have diverse roles during mammalian oogenesis and folliculogenesis. Molecular Human Reproduction, 12(2), 61-69. Joyce, I. M., Pendola, F. L., Wigglesworth, K., & Eppig, J. J. (1999). Oocyte regulation of kit ligand expression in mouse ovarian follicles. Developmental Biology, 214(2), 342-353. Kagawa, H. (2013). Oogenesis in teleost fish. Aqua-BioScience Monographs, 6(4), 99-127. Katakura, F., Yabu, T., Yamaguchi, T., Miyamae, J., Shirinashihama, Y., Nakanishi, T., & Moritomo, T. (2015). Exploring erythropoiesis of common carp (Cyprinus carpio) using an in vitro colony assay in the presence of recombinant carp kit ligand A and erythropoietin. Developmental and Comparative Immunology, 53(1), 13-22. Katzenback, B. A., & Belosevic, M. (2009). Molecular and functional characterization of kita and kitla of the goldfish (Carassius auratus L.). Developmental and Comparative Immunology, 33(11), 1165-1175. Khor, V. K., Ahrends, R., Lin, Y., Shen, W. J., Adams, C. M., Roseman, A. N., Cortez, Y., Teruel, M. N., Azhar, S., & Kraemer, F. B. (2014). The Proteome of Cholesteryl-Ester-Enriched Versus Triacylglycerol-Enriched Lipid Droplets. Plos One, 9(8). Kidder, G. M., & Vanderhyden, B. C. (2010). Bidirectional communication between oocytes and follicle cells: ensuring oocyte developmental competence. Canadian Journal of Physiology and Pharmacology, 88(4), 399-413. Kottler, V. A., Fadeev, A., Weigel, D., & Dreyer, C. (2013). Pigment Pattern Formation in the Guppy, Poecilia reticulata, Involves the Kita and Csf1ra Receptor Tyrosine Kinases. Genetics, 194(3), 631-646. Lai, W. A., Yeh, Y. T., Fang, W. L., Wu, L. S., Harada, N., Wang, P. H., Ke, F. C., Lee, W. L., & Hwang, J. J. (2014). Calcineurin and CRTC2 mediate FSH and TGF beta 1 upregulation of Cyp19a1 and Nr5a in ovary granulosa cells. Journal of Molecular Endocrinology, 53(2), 259-270. Lubzens, E., Young, G., Bobe, J., & Cerda, J. (2010). Oogenesis in teleosts: How fish eggs are formed. General and Comparative Endocrinology, 165(3), 367-389. Motro, B., & Bernstein, A. (1993). Dynamic changes in ovarian c-kit and Steel expression during the estrous reproductive cycle. Dev Dyn, 197(1), 69-79. Nishikawa, S., Kusakabe, M., Yoshinaga, K., Ogawa, M., Hayashi, S., Kunisada, T., Era, T., Sakakura, T., & Nishikawa, S. (1991). In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development. EMBO J, 10(8), 2111-2118. Ohta, H., Kagawa, H., Tanaka, H., Okuzawa, K., Iinuma, N., & Hirose, K. (1997). Artificial induction of maturation and fertilization in the Japanese eel, Anguilla japonica. Fish Physiology and Biochemistry, 17(1), 163-169. Olivereau, M., & Olivereau, J. (1979). Effect of Estradiol-17-Beta on the Cytology of the Liver, Gonads and Pituitary, and on Plasma Electrolytes in the Female Freshwater Eel. Cell and Tissue Research, 199(3), 431-454. Packer, A. I., Hsu, Y. C., Besmer, P., & Bachvarova, R. F. (1994). The Ligand of the C-Kit Receptor Promotes Oocyte Growth. Developmental Biology, 161(1), 194-205. Pang, Y. F., & Ge, W. (2002). Gonadotropin regulation of activin beta A and activin type IIA receptor expression in the ovarian follicle cells of the zebrafish, Danio rerio. Molecular and Cellular Endocrinology, 188(1-2), 195-205. Prat, F., Sumpter, J. P., & Tyler, C. R. (1996). Validation of radioimmunoassays for two salmon gonadotropins (GTH I and GTH II) and their plasma concentrations throughout the reproductive cycle in male and female rainbow trout (Oncorhynchus mykiss). Biology of Reproduction, 54(6), 1375-1382. Ratajczak, M. Z., Luger, S. M., Deriel, K., Abrahm, J., Calabretta, B., & Gewirtz, A. M. (1992). Role of the Kit Protooncogene in Normal and Malignant Human Hematopoiesis. Proceedings of the National Academy of Sciences of the United States of America, 89(5), 1710-1714. Ronnstrand, L. (2004). Signal transduction via the stem cell factor receptor/c-Kit. Cellular and Molecular Life Sciences, 61(19-20), 2535-2548. Sakai, K., Nomura, M., Ishida, O., & Ishii, T. (1972). Studies on the seed production of the Japanese eel - III. Structure of the ovarian oocytes and ovulated eggs. Suisanzoshoku, 19, 201-209. Salamat, N., Erfani Majd, N., Hashemitabar, H., Mesbah, M., & AhangarPour, A. (2012). A Comparative study of synthetic carp GnRH and carp pituitary homogenate effects on in vitro steroidogenesis of oocytes in common carp (Cyprinus carpio). Iranian Journal of Fisheries Sciences, 11(2), 394-404. Sato, T., Yokonishi, T., Komeya, M., Katagiri, K., Kubota, Y., Matoba, S., Ogonuki, N., Ogura, A., Yoshida, S., & Ogawa, T. (2012). Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice. Proceedings of the National Academy of Sciences of the United States of America, 109(42), 16934-16938. Satoh, H., Nakamura, N., & Hibiya, T. (1962). Studies on the sexual maturation of the eel - I. On the sex differentiation and the maturing process of the gonads. Nippon Suisan Gakkaishi, 28, 579-584. Schreiber, J. R., Nakamura, K., Schmit, V., & Weinstein, D. B. (1984). Androgen and Fsh Synergistically Stimulate Lipoprotein Degradation and Utilization by Ovary Granulosa-Cells. Steroids, 43(1), 25-42. Shen, W. J., Azhar, S., & Kraemer, F. B. (2016). Lipid droplets and steroidogenic cells. Experimental Cell Research, 340(2), 209-214. Tanaka, H. (2015). Progression in artificial seedling production of Japanese eel Anguilla japonica. Fisheries Science, 81(1), 11-19. Thomas, F. H., Ethier, J. F., Shimasaki, S., & Vanderhyden, B. C. (2005). Follicle-stimulating hormone regulates oocyte growth by modulation of expression of oocyte and granulosa cell factors. Endocrinology, 146(2), 941-949. Thomas, F. H., & Vanderhyden, B. C. (2006). Oocyte-granulosa cell interactions during mouse follicular development: regulation of kit ligand expression and its role in oocyte growth. Reproductive Biology and Endocrinology, 4. Tse, A. C. K., Lau, K. Y. T., Ge, W., & Wu, R. S. S. (2013). A rapid screening test for endocrine disrupting chemicals using primary cell culture of the marine medaka. Aquatic Toxicology, 144, 50-58. Tsukamoto, K. (1992). Discovery of the Spawning Area for Japanese Eel. Nature, 356(6372), 789-791. Tzeng, W. N. (1990). Relationship between Growth-Rate and Age at Recruitment of Anguilla-Japonica Elvers in a Taiwan Estuary as Inferred from Otolith Growth Increments. Marine Biology, 107(1), 75-81. Tzeng, W. N., Lin, H. R., Wang, C. H., & Xu, S. N. (2000). Differences in size and growth rates of male and female migrating Japanese eels in Pearl River, China. Journal of Fish Biology, 57(5), 1245-1253. Wallace, R. A. (1985). Vitellogenesis and oocyte growth in nonmammalian vertebrates. Dev Biol (N Y 1985), 1, 127-177. Yamamoto, K., & Miura, T. (1988). Sexual maturity and hormone mechanisms in Japanese eel. Marine science monthly, 20, 184-189, (in Japanese). Yamamoto, K., & Yamauchi, K. (1974). Sexual maturation of Japanese eel and production of eel larvae in the aquarium. Nature, 251(5472), 220-222. Yao, K., & Ge, W. (2010). Kit System in the Zebrafish Ovary: Evidence for Functional Divergence of Two Isoforms of Kit (Kita and Kitb) and Kit Ligand (Kitlga and Kitlgb) During Folliculogenesis. Biology of Reproduction, 82(6), 1216-1226. Yao, K., & Ge, W. (2013). Spatial Distribution and Receptor Specificity of Zebrafish Kit System - Evidence for a Kit-Mediated Bi-Directional Communication System in the Preovulatory Ovarian Follicle. Plos One, 8(2). Yeh, J., Lee, G. Y., & Anderson, E. (1993). Presence of Transforming Growth Factor-Alpha Messenger-Ribonucleic-Acid (Messenger-Rna) and Absence of Epidermal Growth-Factor Messenger-Rna in Rat Ovarian Granulosa-Cells, and the Effects of These Factors on Steroidogenesis Invitro. Biology of Reproduction, 48(5), 1071-1081. Yoshida, H., Takakura, N., Kataoka, H., Kunisada, T., Okamura, H., & Nishikawa, S. (1997). Stepwise requirement of c-kit tyrosine kinase in mouse ovarian follicle development. Developmental Biology, 184(1), 122-137. Zhang, H., Risal, S., Gorre, N., Busayavalasa, K., Li, X., Shen, Y., Bosbach, B., Brannstrom, M., & Liu, K. (2014). Somatic Cells Initiate Primordial Follicle Activation and Govern the Development of Dormant Oocytes in Mice. Current Biology, 24(21), 2501-2508. 黃彥融. (2015). 日本鰻早期人工誘導對卵巢發育影響之研究. 臺灣大學漁業科學研究所碩士論文. 詹智堯. (2015). 日本鰻進入最後催熟時機之改進. 臺灣大學漁業科學研究所碩士論文. 廖唯甯. (2015). 日本鰻聯接蛋白Je-Cx34.4與Je-Cx43分子結構隻分析與在卵巢表現之研究. 臺灣大學漁業科學研究所碩士論文. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59799 | - |
| dc.description.abstract | 旁分泌因子Kit ligand (kitlg) 在哺乳類已被廣泛研究，於卵巢組織中主要表現在顆粒細胞 (Granulosa cell)，能夠喚醒休眠的卵細胞並促進生長。本研究驗證人工催熟藥物對日本鰻 (Anguilla japonica) 卵巢kitlga表現的影響，並首次嘗試培養日本鰻濾泡細胞作為實驗基礎。我們選殖出日本鰻之kitlga基因序列，得到cDNA全長1931個鹼基對，預測能轉譯出275個胺基酸。將胺基酸序列與其他物種進行比較並分析親緣關係，結果最接近的是三刺魚 (Gasterosteus aculeatus) 與日本鰻有50%的相同度 (Identity)，其次是大黃魚(Larimichthys crocea) 有48%的相同度。由於鰻魚在人工養殖環境下無法自然達到性成熟，需要施打鮭魚腦下垂體均質液 (Salmon pituitary homogenate, SPH) 來誘導生殖腺發育，因此我們認為人工催熟過程中施打SPH應能刺激kitlga基因表現，進而幫助卵巢繼續發育。為了驗證此假說，我們將日本鰻海水馴化後分為控制組與SPH處理組，進行7針的人工催熟。比較卵巢組織切片，發現人工催熟後僅部分個體進入卵黃蓄積早期，其餘則停留在前卵黃蓄積期。卵巢基因表現方面，kitlga在不同個體的表現差異甚大，各組間無法達到顯著差異。為此本研究嘗試分離並培養日本鰻濾泡細胞，以SPH進行處理觀察基因表現的變化。結果顯示濾泡細胞在高劑量 (1 mg/ml) 的SPH處理後可顯著增加cyp19a1 mRNA表現，卻有明顯抑制kitlga mRNA的效果。我們的結果得知注射SPH確實能刺激類固醇生合成 (Steroidogenesis)，但也發現卵子生成作用 (Oogenesis) 可能因kitlga表現下降而受到抑制，間接影響卵濾泡的生長。至於SPH為何會影響kitlga的基因表現則有待進一步研究。 | zh_TW |
| dc.description.abstract | Japanese eel, Anguilla japonica, is one of economical animal in Asian aquaculture. The reproductive mechanism and strategies of Japanese eel are still unclear, and salmon pituitary homogenate (SPH) induction is necessary to promote ovarian follicle growth. Recently, kit ligand (kitlg) is mainly secreted by the granulosa cells of the ovarian tissue and identified as a cytokine to initiate primordial follicle activation and govern the development of dormant oocytes. However, the precise function and characteristics of kit ligand in the reproduction of Japanese eel remain unclear. This study assumed that the exogenous SPH can stimulate kitlga expression to promote the ovary development, therefore, we characterized the expression patterns of kitlga in the Japanese eel. To investigate the effect of kitlga on the reproductive physiology of Japanese eel, the in vivo and in vitro studies were performed in this research. Japanese female eel were intraperitoneal injected with 7 weekly SPH induction, and the isolated follicular cell from ovary was also established a cell culture platform to investigate the effect of SPH on kitlga gene expression. First, we cloned kitlga cDNA form ovary by reserve transcription polymerase chain reaction (RT-PCR) followed by rapid amplification of cDNA ends (RACE). Next, in order to examine the effect of SPH induction on kitlga expression, we analyzed expression pattern of kitlga messenger RNA (mRNA) by real-time quantitative PCR (qPCR) in ovary tissue and follicular cells. We cloned the full length (1931bp) of kitlga cDNAs of Japanese eel ovary, and the amino acid sequence (275 a.a.) shows high identity with that of other reported teleost kitlga. The results of qPCR found that kitlga gene expression were not significant differences in the control- and SPH group after 7 weekly inductions, and the histological section indicated that the development stage of ovaries has an individual differences. Follicular cells treated with SPH of different concentrations have showed that SPH at a high concentration (1 mg/ml) enhanced the expression of key molecule of steroidogenesis such as cyp19a1 (P450 aromatase), however, the kitlga expression was down-regulated. These results reveal that SPH can promote ovarian steroidogenesis, but the decreased kitlga may have a different role in the ovary development. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T09:38:35Z (GMT). No. of bitstreams: 1 ntu-105-R03b45001-1.pdf: 3634275 bytes, checksum: 06a81fbc31940b6064a2526b8a943b70 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
謝辭 II 中文摘要 III Abstract IV 第一章 前言 1 1.1 日本鰻的經濟價值與資源困境 1 1.2 日本鰻的生活史 1 1.3 日本鰻人工誘導催熟 2 1.4 日本鰻的卵子生成 (Oogenesis) 3 1.5 Kit Ligand在卵巢發育中扮演的角色 4 1.6 研究動機與實驗設計 5 第二章 材料與方法 7 2.1 日本鰻kitlga基因選殖 7 2.1.1 5’cDNA末端快速擴增反應 (5’ RACE) 7 2.1.2 3’cDNA末端快速擴增反應 (3’ RACE) 7 2.1.3 DNA片段定序 7 2.2 活體實驗動物處理與採樣 8 2.3 石蠟切片 9 2.3.1 組織固定保存 9 2.3.2 組織包埋與切片 9 2.3.3 H & E染色 9 2.4 濾泡細胞培養與細胞實驗藥物處理 10 2.4.1 初代細胞分離與培養 10 2.4.2 細胞冷凍保存 10 2.4.3 細胞繼代 10 2.4.4 細胞實驗藥物處理 11 2.5 基因表現量分析 11 2.5.1 總量RNA萃取 11 2.5.2 cDNA製備 (Reverse transcription) 11 2.5.3 即時定量聚合酶鏈鎖反應 (qPCR) 12 2.5.4 檢測之基因 12 2.6 統計分析 12 第三章 實驗結果 14 3.1 日本鰻kitlga基因序列分析 14 3.2 人工誘導對日本鰻性腺發育之影響 14 3.3 卵巢組織形質觀察 15 3.4 日本鰻卵巢不同時期之基因表現 15 3.5 日本鰻kitlga於不同組織的基因表現 15 3.6 SPH對濾泡細胞形質上的影響 16 3.7 SPH處理濾泡細胞對基因表現的影響 16 第四章 討論 17 4.1 Kit ligand基因分型 17 4.2 日本鰻人工誘導對卵巢之影響 17 4.3 kitlga在卵巢發育中表現量的變化 18 4.4 濾泡細胞形質 19 第五章 結論 21 參考資料 22 附表 27 附圖 28 | |
| dc.language.iso | zh-TW | |
| dc.subject | 人工誘導 | zh_TW |
| dc.subject | 細胞培養 | zh_TW |
| dc.subject | Kit ligand | zh_TW |
| dc.subject | 日本鰻 | zh_TW |
| dc.subject | 卵巢發育 | zh_TW |
| dc.subject | ovary development | en |
| dc.subject | cell culture | en |
| dc.subject | kit ligand | en |
| dc.subject | Japanese eel | en |
| dc.subject | artificial induction | en |
| dc.title | 日本鰻卵巢發育過程中Kit-Ligand基因表現之研究 | zh_TW |
| dc.title | Expression of Kit Ligand in the Ovarian Development of Japanese eel (Anguilla japonica) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 廖文亮,李孟芳,曾登裕 | |
| dc.subject.keyword | 日本鰻,人工誘導,卵巢發育,Kit ligand,細胞培養, | zh_TW |
| dc.subject.keyword | Japanese eel,artificial induction,ovary development,kit ligand,cell culture, | en |
| dc.relation.page | 44 | |
| dc.identifier.doi | 10.6342/NTU201700193 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-02-10 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-105-1.pdf 未授權公開取用 | 3.55 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。