請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59676
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 許如君 | |
dc.contributor.author | Po-Kai Hsu | en |
dc.contributor.author | 許博凱 | zh_TW |
dc.date.accessioned | 2021-06-16T09:32:43Z | - |
dc.date.available | 2022-02-20 | |
dc.date.copyright | 2017-02-20 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-02-14 | |
dc.identifier.citation | 李文蓉。1998。東方果實蠅之防治。中華昆蟲特刊 2:51-60。
程建中、李文蓉。1998。環境對東方果實蠅族群之影響。劉玉章、陳昭鈞編著。台灣果實蠅防治技術研討會專刊。國立中興大學昆蟲學系,台灣。pp 36-43。 劉玉章、黃莉欣。1990。東方果實蠅之產卵偏好。中華昆蟲特刊 10:159-168。 Aketarawong N, Bonizzoni M, Thanaphum S, Gomulski LM, Gasperi G, Malacrida AR, Gugliemino CR. 2007. Inferences on the population structure and colonization process of the invasive oriental fruit fly, Bactrocera dorsalis (Hendel). Mol Ecol 16: 3522-3532. Aldridge WN. 1953. Serum esterases. I. 2 types of esterase (a and b) hydrolysing para-nitrophenyl acetate, propionate and butyrate, and a method for their determination. Biochem J 53: 110-117. Aldridge WN, Reiner E. 1972. Enzyme Inhibitors as Substrates. Interactions of Esterases with Esters of Organophosphorus and Carbamic Acids. Amsterdam: North-Holland Publishing. Artem EM, Peter W, Kirby S, Susan F. 2008. Sanger DNA sequencing. pp 1-11. In: Michael J. (ed). Next-Generation Genome Sequencing: Towards Personalized Medicine. Weinheim, Germany. Asperen KV. 1962. A study of house fly esterases by means of a sensitive colorimetric method. J Insect Physiol: 401-416. Bentley DR. 2006. Whole-genome re-sequencing. Curr Opin Genet Dev 16: 545-552. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254. Buermans HPJ, den Dunnen JT. 2014. Next generation sequencing technology: advances and applications. Bba-Mol Basis Dis 1842: 1932-1941. Busvine JR. 1979. Recommend methods for the detection and measurement of resistance of agricultural pests to pesticides: Method for tephritid fruit flies-FAO method no. 20. FAO Plant Prot Bull 27: 40-43. California Department of Food and Agriculture. 2013. Insect Trapping Guide, 13th Edition. Sacramento, California: State of California, Department of Food and Agriculture, Pest Detection/Emergency Project. Campbell PM, Newcomb RD, Russell RJ, Oakeshott JG. 1998. Two different amino acid substitutions in the ali-esterase, E3, confer alternative types of organophosphorus insecticide resistance in the sheep blowfly, Lucilia cuprina. Insect Biochem Mol Biol 28: 139-150. Campbell PM, Robin GCD, Court LN, Dorrian SJ, Russell RJ, Oakeshott JG. 2003. Developmental expression and gene/enzyme identifications in the alpha esterase gene cluster of Drosophila melanogaster. Insect Mol Biol 12: 459-471. Chiu HT. 1978. Studies on the improvement of mass rearing for oriental fruit flies. Plant Prot Bull 20: 87-92. Christenson LD, Foote RH. 1960. Biology of fruit flies. Annu Rev Entomol 5: 171-192. Clarke AR, Armstrong KF, Carmichael AE, Milne JR, Raghu S, Roderick GK, Yeates DK. 2005. Invasive phytophagous pests arising through a recent tropical evolutionary radiation: The Bactrocera dorsalis complex of fruit flies. Annu Rev Entomol 50: 293-319. Claudianos C, Ranson H, Johnson RM, Biswas S, Schuler MA, Berenbaum MR, Feyereisen R, Oakeshott JG. 2006. A deficit of detoxification enzymes: pesticide sensitivity and environmental response in the honeybee. Insect Mol Biol 15: 615-636. Claudianos C, Russell RJ, Oakeshott JG. 1999. The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly. Insect Biochem Mol Biol 29: 675-686. Coates PM, Mestriner MA, Hopkinson DA. 1975. Preliminary genetic interpretation of esterase isoenzymes of human tissues. Ann Hum Genet 39: 1-20. Contreras-Gomez A, Sanchez-Miron A, Garcia-Camacho F, Molina-Grima E, Chisti Y. 2014. Protein production using the baculovirus-insect cell expression system. Biotechnol Prog 30: 1-18. Corbett JR. 1974. Insecticides inhibiting acetylcholinesterase. pp 107-164. In: Corbett J. R. (ed). The Biochemical Mode of Action of Pesticides. Academic Press, London ; New York. De Villiers M, Hattingh V, Kriticos DJ, Brunel S, Vayssieres JF, Sinzogan A, Billah MK, Mohamed SA, Mwatawala M, Abdelgader H, Salah FEE, De Meyer M. 2016. The potential distribution of Bactrocera dorsalis: considering phenology and irrigation patterns. Bull Entomol Res 106: 19-33. Devonshire AL. 1977. The properties of a carboxylesterase from the peach-potato aphid, Myzus persicae (Sulz.), and its role in conferring insecticide resistance. Biochem J 167: 675-683. Devonshire AL, Heidari R, Bell KL, Campbell PM, Campbell BE, Odgers WA, Oakeshott JG, Russell RJ. 2003. Kinetic efficiency of mutant carboxylesterases implicated in organophosphate insecticide resistance. Pestic Biochem Physiol 76: 1-13. Devonshire AL, Moores GD. 1982. A carboxylesterase with broad substrate-specificity causes organo-phosphorus, carbamate and pyrethroid resistance in peach-potato aphids (Myzus persicae). Pestic Biochem Physiol 18: 235-246. Devonshire AL, Moores GD. 1989. Detoxication of insecticides by esterases from Myzus persicae: is hydrolysis important? pp 180-192. Esterases Hydrolysing Organophosphorus Compounds. Ellis Horwood, Chichester. Drew RAI, Hancock DL. 2000. Phylogeny of the Tribe Dacini (Dacinae) based on morphological, distributional, and biological data. pp 491-533. In: Aluja M., Norrbom A. L. (eds). Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton, FL, USA. Ellison CA, Crane AL, Olson JR. 2012. Biotransformation of insecticides. pp 685-702. In: Anzenbacher P., Zanger U. M. (eds). Metabolism of Drugs and Other Xenobiotics. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. Eto M. 1974. Organophosphorus Pesticides: Organic and Biological Chemistry. Cleveland, Ohio: CRC Press. 387 pp. Feyereisen R. 1995. Molecular biology of insecticide resistance. Toxicol Lett 82-3: 83-90. Field LM, Anderson AP, Denholm I, Foster SP, Harling ZK, Javed N, MartinezTorres D, Moores GD, Williamson MS, Devonshire AL. 1997. Use of biochemical and DNA diagnostics for characterising multiple mechanisms of insecticide resistance in the peach-potato aphid, Myzus persicae (sulzer). Pestic Sci 51: 283-289. Field LM, Devonshire AL. 1998. Evidence that the E4 and FE4 esterase genes responsible for insecticide resistance in the aphid Myzus persicae (Sulzer) are part of a gene family. Biochem J 330: 169-173. Field LM, Williamson MS, Moores GD, Devonshire AL. 1993. Cloning and analysis of the esterase genes conferring insecticide resistance in the peach-potato aphid, Myzus persicae (Sulzer). Biochem J 294: 569-574. Geib SM, Calla B, Hall B, Hou S, Manoukis NC. 2014. Characterizing the developmental transcriptome of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) through comparative genomic analysis with Drosophila melanogaster utilizing modENCODE datasets. BMC Genomics [Internet]. 2014 15: 942. Available from: doi: 10.1186/1471-2164-15-942. Gupta R, Brunak S. 2002. Prediction of glycosylation across the human proteome and the correlation to protein function. Pacific Symposium on Biocomputing Pacific Symposium on Biocomputing: 310-322. Harel M, Kryger G, Rosenberry TL, Mallender WD, Lewis T, Fletcher RJ, Guss JM, Silman I, Sussman JL. 2000. Three-dimensional structures of Drosophila melanogaster acetylcholinesterase and of its complexes with two potent inhibitors. Protein Sci 9: 1063-1072. Hawkes NJ, Janes RW, Hemingway J, Vontas J. 2005. Detection of resistance-associated point mutations of organophosphate-insensitive acetylcholinesterase in the olive fruit fly, Bactrocera oleae (Gmelin). Pestic Biochem Physiol 81: 154-163. Healy MJ, Dumancic MM, Oakeshott JG. 1991. Biochemical and physiological studies of soluble esterases from Drosophila melanogaster. Biochem Genet 29: 365-388. Hemingway J, Coleman M, Paton M, McCarroll L, Vaughan A, DeSilva D. 2000. Aldehyde oxidase is coamplified with the World's most common Culex mosquito insecticide resistance-associated esterases. Insect Mol Biol 9: 93-99. Hemingway J, Hawkes NJ, McCarroll L, Ranson H. 2004. The molecular basis of insecticide resistance in mosquitoes. Insect Biochem Mol Biol 34: 653-665. Heukeshoven J, Dernick R. 1985. Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6: 103-112. Holmes RS, Masters CJ. 1967. Developmental multiplicity and isoenzyme status of cavian esterases. Biochimica Et Biophysica Acta 132: 379-399. Hsu JC, Chien TY, Hu CC, Chen MJM, Wu WJ, Feng HT, Haymer DS, Chen CY. 2012. Discovery of genes related to insecticide resistance in Bactrocera dorsalis by functional genomic analysis of a de novo assembled transcriptome. PLoS ONE [Internet]. 2012 7(8): e40950. Available from: doi: 10.1371/journal.pone.0040950. Hsu JC, Feng HT. 2000. Insecticide susceptibility of the oriental fruit fly (Bactrocera dorsalis (Hendel)) (Diptera: Tephritidae) in Taiwan. Chinese J Entomol 20: 109-118. Hsu JC, Feng HT. 2002. Susceptibility of melon fly (Bactrocera cucurbitae) and oriental fruit fly (B. dorsalis) to insecticides in Taiwan. Plant Prot Bull 44: 303-314. Hsu JC, Feng HT, Haymer DS, Chen YH. 2011. Molecular and biochemical mechanisms of organophosphate resistance in laboratory-selected lines of the oriental fruit fly (Bactrocera dorsalis). Pestic Biochem Physiol 100: 57-63. Hsu JC, Feng HT, Wu WJ. 2004. Resistance and synergistic effects of insecticides in Bactrocera dorsalis (Diptera: Tephritidae) in Taiwan. J Econ Entomol 97: 1682-1688. Hsu JC, Haymer DS, Wu WJ, Feng HT. 2006. Mutations in the acetylcholinesterase gene of Bactrocera dorsalis associated with resistance to organophosphorus insecticides. Insect Biochem Mol Biol 36: 396-402. Hsu JC, Huang LH, Feng HT, Su WY. 2015. Do organophosphate-based traps reduce control efficiency of resistant tephritid flies? J Pest Sci 88: 181-190. Hsu PK, Huang LH, Geib SM, Hsu JC. 2016. Identification of a carboxylesterase associated with resistance to naled in Bactrocera dorsalis (Hendel). Pestic Biochem Physiol 131: 24-31. Hu YC. 2005. Baculovirus as a highly efficient expression vector in insect and mammalian cells. Acta Pharmacol Sin 26: 405-416. International Atomic Energy Agency. 2003. Trapping guidelines for area-wide fruit fly programmes. Vienna: The Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Jackson CJ, Liu J-W, Carr PD, Younus F, Coppin C, Meirelles T, Lethier M, Pandey G, Ollis DL, Russell RJ, Weik M, Oakeshott JG. 2013. Structure and function of an insect alpha-carboxylesterase (alpha esterase7) associated with insecticide resistance. Proceedings of the National Academy of Sciences of the United States of America 110: 10177-10182. Jiang JJ, Zhou K, Liang GW, Zeng L, Wen SY. 2014. A novel point mutation of acetylcholinesterase in a trichlorfon-resistant strain of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae). Appl Entomol Zool 49: 129-137. Jin T, Zeng L, Lin YY, Lu YY, Liang GW. 2011. Insecticide resistance of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), in mainland China. Pest Manage Sci 67: 370-376. Kakani EG, Ioannides IM, Margaritopoulos JT, Seraphides NA, Skouras PJ, Tsitsipis JA, Mathiopoulos KD. 2008. A small deletion in the olive fly acetylcholinesterase gene associated with high levels of organophosphate resistance. Insect Biochem Mol Biol 38: 781-787. Karunaratne SHPP, Hemingway J, Jayawardena KGI, Dassanayaka V, Vaughan A. 1995. Kinetic and molecular differences in the amplified and non-amplified esterases from insecticide-resistant and susceptible Culex quinquefasciatus mosquitoes. J Biol Chem 270: 31124-31128. Karunaratne SHPP, Jayawardena KGI, Hemingway J, Ketterman AJ. 1993. Characterization of a B-type esterase involved in insecticide resistance from the mosquito Culex quinquefasciatus. Biochem J 294: 575-579. Khow O, Suntrarachun S. 2012. Strategies for production of active eukaryotic proteins in bacterial expression system. Asian Pac J Trop Biomed 2: 159-162. Krimbas CB, Tsakas S. 1971. The genetics of Dacus oleae. IV. Changes of esterase polymorphism in a natural population following insecticide control-selection or drift? Evolution 25: 454-460. Kuo TCY, Hu CC, Chien TY, Chen MJM, Feng HT, Chen LFO, Chen CY, Hsu JC. 2015. Discovery of genes related to formothion resistance in oriental fruit fly (Bactrocera dorsalis) by a constrained functional genomics analysis. Insect Mol Biol 24: 338-347. Leblanc L, Putoa R. 2000. Fruit flies in French Polynesia and Pitcairn Islands. Suva, Fiji.: Secretariat of the Pacific Community Pest Advisory leaflet. 4 pp. LeOra-software. 1987. Polo-Pc: A User's Guide to Probit or Logit Analysis. Berkeley, CA: LeOra software. Li Q, Chen R, Li W, Qiao C-L, Wu Y-J. 2007a. A genetically engineered Escherichia coli, expressing the fusion protein of green fluorescent protein and carboxylesterase B1, can be easily detected in the environment following degradation of pesticide residues. Biotechnol Lett 29: 1357-1362. Li XC, Schuler MA, Berenbaum MR. 2007b. Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics. Annu Rev Entomol 52: 231-253. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408. Lopez A, Yusa V, Munoz A, Vera T, Borras E, Rodenas M, Coscolla C. 2017. Risk assessment of airborne pesticides in a Mediterranean region of Spain. Sci Total Environ 574: 724-734. Magana C, Hernandez-Crespo P, Brun-Barale A, Couso-Ferrer F, Bride J-M, Castanera P, Feyereisen R, Ortego F. 2008. Mechanisms of resistance to malathion in the medfly Ceratitis capitata. Insect Biochem Mol Biol 38: 756-762. Magana C, Hernandez-Crespo P, Ortego F, Castanera P. 2007. Resistance to malathion in field populations of Ceratitis capitata. J Econ Entomol 100: 1836-1843. Mardis ER. 2008a. Next-generation DNA sequencing methods. Annu Rev Genom Hum G 9: 387-402. Mardis ER. 2008b. The impact of next-generation sequencing technology on genetics. Trends Genet 24: 133-141. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen ZT, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer MLI, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu PG, Begley RF, Rothberg JM. 2005. Genome sequencing in microfabricated high-density picolitre reactors. Nature 437: 376-380. Metcalf RL, Metcalf ER. 1992. Fruit flies of the family Tephritidae. pp 109-152. In: Metcalf R. L., Metcalf E. R. (eds). Plant Kairomones in Insect Ecology and Control. Chapman and Hall Press, New York. Montella IR, Schama R, Valle D. 2012. The classification of esterases: an important gene family involved in insecticide resistance - a review. Mem Inst Oswaldo Cruz 107: 437-449. Mutero A, Pralavorio M, Bride JM, Fournier D. 1994. Resistance-associated point mutations in insecticide-insensitive acetylcholinesterase. Proceedings of the National Academy of Sciences of the United States of America 91: 5922-5926. Needham PH, Sawicki RM. 1971. Diagnosis of resistance to organophosphorus insecticides in Myzus persicae (Sulz.). Nature 230: 125-126. Oakeshott JG, Claudianos C, Campbell PM, Newcomb RD, Russell RJ. 2005. Biochemical genetics and genomics of insect esterases. pp 309-381. In: Gilbert Lawrence I., Latrou K., Gill Sarjeet S. (eds). Comprehensive Molecular Insect Science - Pharmacology. Elsevier, Oxford, U.K. Oakeshott JG, Claudianos C, Campbell PM, Newcomb RD, Russell RJ. 2010a. Biochemical genetics and genomics of insect esterases. pp 229-301. In: Gilbert Lawrence I., Gill Sarjeet S. (eds). Insect Pharmacology: Channels, Receptors, Toxins and Enzymes. Elsevier, London, U.K. Oakeshott JG, Johnson RM, Berenbaum MR, Ranson H, Cristino AS, Claudianos C. 2010b. Metabolic enzymes associated with xenobiotic and chemosensory responses in Nasonia vitripennis. Insect Mol Biol 19: 147-163. Oppenoorth FJ, Vanasperen K. 1960. Allelic genes in the housefly producing modified enzymes that cause organophosphate resistance. Science 132: 298-299. Palomares LA, Estrada-Moncada S, Ramírez OT. 2004. Production of recombinant proteins. pp 15-51. In: Balbás Paulina, Lorence Argelia (eds). Recombinant Gene Expression: Reviews and Protocols. Humana Press, Totowa, NJ. Papanicolaou A, Schetelig MF, Arensburger P, Atkinson PW, Benoit JB, Bourtzis K, Castanera P, Cavanaugh JP, Chao H, Childers C, Curril I, Dinh H, Doddapaneni H, Dolan A, Dugan S, Friedrich M, Gasperi G, Geib S, Georgakilas G, Gibbs RA, Giers SD, Gomulski LM, Gonzalez-Guzman M, Guillem-Amat A, Han Y, Hatzigeorgiou AG, Hernandez-Crespo P, Hughes DS, Jones JW, Karagkouni D, Koskinioti P, Lee SL, Malacrida AR, Manni M, Mathiopoulos K, Meccariello A, Murali SC, Murphy TD, Muzny DM, Oberhofer G, Ortego F, Paraskevopoulou MD, Poelchau M, Qu J, Reczko M, Robertson HM, Rosendale AJ, Rosselot AE, Saccone G, Salvemini M, Savini G, Schreiner P, Scolari F, Siciliano P, Sim SB, Tsiamis G, Urena E, Vlachos IS, Werren JH, Wimmer EA, Worley KC, Zacharopoulou A, Richards S, Handler AM. 2016. The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species. Genome Biol [Internet]. 2016 17(1): 192. Available from: doi: 10.1186/s13059-016-1049-2. Pavlidi N, Dermauw W, Rombauts S, Chrisargiris A, Van Leeuwen T, Vontas J. 2013. Analysis of the olive fruit fly Bactrocera oleae transcriptome and phylogenetic classification of the major detoxification gene families. PLoS One [Internet]. 2013 8(6): e66533. Available from: doi: 10.1371/journal.pone.0128056. Petersen TN, Brunak S, von Heijne G, Nielsen H. 2011. SignalP 4.0: Discriminating Signal Peptides from Transmembrane Regions. Nat Methods 8: 785-786. Petras ML. 1963. Genetic control of a serum esterase component in Mus musculus. Proc Natl Acad Sci USA 50: 112-116. Pierleoni A, Martelli PL, Casadio R. 2008. PredGPI: A GPI-Anchor Predictor. BMC Bioinformatics [Internet]. 2008 9: 392. Available from: doi: 10.1186/1471-2105-9-392. Qiao CL, Shen BC, Xing JM, Huang J, Zhang JL, Zhao DX, Yang B. 2006. Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1. Int Biodeterior Biodegrad 58: 77-81. Ranson H, Claudianos C, Ortelli F, Abgrall C, Hemingway J, Sharakhova MV, Unger MF, Collins FH, Feyereisen R. 2002. Evolution of supergene families associated with insecticide resistance. Science 298: 179-181. Reed LJ, Muench H. 1938. A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493-497. Roberts JR, Reigart JR. 2013. Organophosphate insecticides. pp 43-55. Recognition and Management of Pesticide Poisonings. U.S. Environmental Protection Agency, Washington, DC. Shen GM, Dou W, Huang Y, Jiang XZ, Smagghe G, Wang JJ. 2013. In silico cloning and annotation of genes involved in the digestion, detoxification and RNA interference mechanism in the midgut of Bactrocera dorsalis [Hendel (Diptera: Tephritidae)]. Insect Mol Biol 22: 354-365. Shen GM, Dou W, Niu JZ, Jiang HB, Yang WJ, Jia FX, Hu F, Cong L, Wang JJ. 2011. Transcriptome Analysis of the Oriental Fruit Fly (Bactrocera dorsalis). Plos One [Internet]. 2011 6(12): e29127. Available from: doi: 10.1371/journal.pone.0029127. Shendure J, Ji HL. 2008. Next-generation DNA sequencing. Nat Biotechnol 26: 1135-1145. Shi MA, Lougarre A, Alies C, Fremaux I, Tang ZH, Stojan J, Fournier D. 2004. Acetylcholinesterase alterations reveal the fitness cost of mutations conferring insecticide resistance. BMC Evol Biol [Internet]. 2004 4: 5. Available from: doi: 10.1186/1471-2148-4-5. Siegfried BD, Scharf ME. 2001. Mechanisms of organophosphate resistance in insects. pp 269-291. In: Ishaaya Isaac (ed). Biochemical Sites of Insecticide Action and Resistance. Springer-Verlag Heidelberg, Germany. Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, Li WZ, Lopez R, McWilliam H, Remmert M, Soding J, Thompson JD, Higgins DG. 2011. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol [Internet]. 2011 7: 539. Available from: doi: 10.1038/Msb.2011.75. Spackman ME, Oakeshott JG, Smyth KA, Medveczky KM, Russell RJ. 1994. A cluster of esterase genes on chromosome 3R of Drosophila melanogaster includes homologs of esterase genes conferring insecticide resistance in Lucilia cuprina. Biochem Genet 32: 39-62. Stasinakis P, Katsares V, Mavragani-Tsipidou P. 2001. Organophosphate resistance and allelic frequencies of esterases in the olive fruit fly Bactrocera oleae (Diptera : Tephritidae). J Agric Urban Entomol 18: 157-168. Stephens AEA, Kriticos DJ, Leriche A. 2007. The current and future potential geographical distribution of the oriental fruit fly, Bactrocera dorsalis (Diptera : Tephritidae). Bull Entomol Res 97: 369-378. Strode C, Wondji CS, David JP, Hawkes NJ, Lumjuan N, Nelson DR, Drane DR, Karunaratne SHPP, Hemingway J, Black WC, Ranson H. 2008. Genomic analysis of detoxification genes in the mosquito Aedes aegypti. Insect Biochem Mol Biol 38: 113-123. TACTRI. 2015. Plant Protection Manual. Taichung, Taiwan: Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture. 319 pp. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol Biol Evol 30: 2725-2729. Teese MG, Farnsworth CA, Li Y, Coppin CW, Devonshire AL, Scott C, East P, Russell RJ, Oakeshott JG. 2013. Heterologous expression and biochemical characterisation of fourteen esterases from helicoverpa armigera. Plos One [Internet]. 2013 8(6): e65951. Available from: doi: 10.1371/journal.pone.0065951. Thompson FC. 1998. Fruit Fly Expert Identification System and Systematic Information Database. Leiden, Netherlands: Backhuys Publishers. 524 pp. Tsakas S, Krimbas CB. 1970. The genetics of Dacus oleae. IV. Relation between adult esterase genotypes and survival to organophosphate insecticides. Evolution 24: 807-815. van Dijk EL, Auger H, Jaszczyszyn Y, Thermes C. 2014. Ten years of next-generation sequencing technology. Trends Genet 30: 418-426. van Wendel de Joode B, Mora AM, Lindh CH, Hernandez-Bonilla D, Cordoba L, Wesseling C, Hoppin JA, Mergler D. 2016. Pesticide exposure and neurodevelopment in children aged 6-9 years from Talamanca, Costa Rica. Cortex 85: 137-150. Voet D, Voet, J. G. 1990. Biochemistry. New York: Wiley. 1520 pp. Vontas J, Hernández-Crespo P, Margaritopoulos JT, Ortego F, Feng H-T, Mathiopoulos KD, Hsu J-C. 2011. Insecticide resistance in tephritid flies. Pestic Biochem Physiol 100: 199-205. Vontas JG, Cosmidis N, Loukas M, Tsakas S, Hejazi MJ, Ayoutanti A, Hemingway J. 2001. Altered acetylcholinesterase confers organophosphate resistance in the olive fruit fly Bactrocera oleae. Pestic Biochem Physiol 71: 124-132. Vontas JG, Hejazi MJ, Hawkes NJ, Cosmidis N, Loukas M, Hemingway J. 2002. Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly Bactrocera oleae. Insect Mol Biol 11: 329-336. Walker CH, Mackness MI. 1983. Esterases: problems of identification and classification. Biochem Pharmacol 32: 3265-3269. Wang LL, Huang Y, Lu XP, Jiang XZ, Smagghe G, Feng ZJ, Yuan GR, Wei D, Wang JJ. 2015a. Overexpression of two alpha-esterase genes mediates metabolic resistance to malathion in the oriental fruit fly, Bactrocera dorsalis (Hendel). Insect Mol Biol 24: 467-479. Wang LL, Lu XP, Meng LW, Huang Y, Wei D, Jiang HB, Smagghe G, Wang JJ. 2015b. Functional characterization of an α-esterase gene involving malathion detoxification in Bactrocera dorsalis (Hendel). Pestic Biochem Physiol 130: 44-51. White IM, Elson-Harris MM. 1992. Fruit Flies of Economic Significance: Their Identification and Bionomics. Wallingford, United Kingdom: CAB International. Wilkins MR, Gasteiger E, Bairoch A, Sanchez JC, Williams KL, Appel RD, Hochstrasser DF. 1999. Protein identification and analysis tools in the ExPASy server. Methods in molecular biology 112: 531-552. Wilson BW. 2010. Cholinesterases. pp 1457-1478. In: Krieger Robert (ed). Hayes' Handbook of Pesticide Toxicology, 3rd Edition. Academic Press, New York. Worek F, Thiermann H, Wille T. 2016. Oximes in organophosphate poisoning: 60 years of hope and despair. Chem Biol Interact 259: 93-98. Xu H, Freitas MA. 2009. MassMatrix: A database search program for rapid characterization of proteins and peptides from tandem mass spectrometry data. Proteomics 9: 1548-1555. Yang WJ, Yuan GR, Cong L, Xie YF, Wang JJ. 2014. De novo cloning and annotation of genes associated with immunity, detoxification and energy metabolism from the fat body of the oriental fruit fly, Bactrocera dorsalis. Plos One [Internet]. 2014 9(4): e94470. Available from: doi: 10.1371/journal.pone.0094470. Yin J, Zhong T, Wei Z-J, Li K-B, Cao Y-Z, Guo W. 2011. Molecular characters and recombinant expression of the carboxylesterase gene of the meadow moth Loxostege sticticalis L. (Lepidoptera: Pyralidae). African Journal of Biotechnology 10: 1794-1801. Yu QY, Lu C, Li WL, Xiang ZH, Zhang Z. 2009. Annotation and expression of carboxylesterases in the silkworm, Bombyx mori. BMC Genomics [Internet]. 2009 10: 553. Available from: doi: Doi 10.1186/1471-2164-10-553. Zhang JL, Qiao CL, Lan WS. 2005. Purification and some characteristics of recombinant insecticide-resistant mosquito carboxylesterase B1 expressed in Escherichia coli. Enzyme Microb Technol 36: 648-653. Zhang Y, Zeng L, Lu Y, Liang G. 2008. Monitoring of insecticides resistance of oriental fruit fly field populations in South China. J Huazhong Agric Univ 27: 456-459. Zheng YZ, Lan WS, Qiao CL, Mulchandani A, Chen W. 2007. Decontamination of vegetables sprayed with organophosphate pesticides by organophosphorus hydrolase and carboxylesterase (B1). Appl Biochem Biotechnol 136: 233-241. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59676 | - |
dc.description.abstract | 羧酸酯酶 (carboxylesterase, CBE) 具有水解羧酸酯及隔離殺蟲劑等外來物質之生理功能,為昆蟲外來物防禦系統的重要組成,並於東方果實蠅 (Bactrocera dorsalis) 與其他昆蟲中參與對有機磷類 (organophosphate) 殺蟲劑之抗藥性機制。先前研究指出東方果實蠅乃力松抗性品系與其他有機磷抗性以及感性品系相比,在酯酶活性圖譜上有一特定高強度之羧酸酯酶條帶 BdE5,且可被氧化巴拉松與乃力松等有機磷劑抑制,其在族群中出現頻度亦證實與乃力松抗藥性有關。在本研究中,體內抑制實驗發現診斷劑量之乃力松對於感性品系酯酶之總體抑制程度影響較抗性品系大,透過 NanoLC-nanoESi-MS/MS 針對 BdE5 進行蛋白質鑑定並將其註解為 esterase FE4-like (XP_011200445.1),利用快速擴增 cDNA 末端法 (rapid amplification of cDNA ends) 取得其 2,012 bp 之 cDNA 全長序列,並包含長度為1,770 bp 且可轉譯 590 個胺基酸殘基之開放閱讀框架 (open reading frame)。親緣分析亦指出其屬於分泌性 β 酯酶 (secreted β esterases)(E 支群) 並與黑腹果蠅 (Drosophila melanogaster) 之羧酸酯酶 CG6414 (NP_570089) 具有親緣關係。另一方面,由於昆蟲羧酸酯酶基因屬於羧酸/膽鹼酯酶 (carboxyl/cholinesterase, CCE) 多基因家族,許多與有機磷抗性相關之羧酸酯酶仍尚未被發現與個別探討。為發掘更多有機磷抗藥性相關之羧酸酯酶,本研究透過 RNA 測序 (RNA sequencing, RNA-seq) 取得東方果實蠅轉錄體資訊進行分析,以 5 個有機磷抗性品系對感性品系間轉錄本 (transcript) 之 RPKM (reads per kilobase per million mapped reads) 比值篩選,由 33 條酯酶基因中選取 6 條比值大於 2 之候選基因。以即時定量聚合酶連鎖反應 (RT-qPCR) 測定候選基因於乃力松、三氯松及馬拉松抗性品系比對感性品系計算所得之基因相對表現量,結果顯示其中 3 條在 3 個抗性品系中表現量皆顯著高於感性品系 (或高於 2 倍),可能與有機磷抗藥性相關。BdE5 基因亦為其中之一,與先前其在酯酶圖譜及活性實驗之發現相符。而相對表現量最高之基因被註解為 carboxylesterase B2 (AGU42836.1)(為 alpha-Esterase-7 (αE7) 之直系同源基因),最終亦成功於秋行軍蟲 (Spodoptera frugiperda) Sf21 細胞中,以昆蟲桿狀病毒 (baculovirus) 表現系統表現出具有活性之 αE7 重組蛋白。本研究提供對東方果實蠅有機磷抗藥性相關之羧酸酯酶更深入之認識,並生產東方果實蠅 αE7 重組蛋白供後續抗藥性機制研究或相關技術開發之應用。 | zh_TW |
dc.description.abstract | Physiological functions of carboxylesterases (CBEs) include hydrolysis of carboxylic esters and sequestration of xenobiotics such as insecticides, making CBEs the most important component of the xenobiotic defense system in insects. CBEs are also involved in the mechanism of organophosphorus insecticide resistance in many insects such as Bactrocera dorsalis. Previous studies have compared organophosphate (OP)-resistant and -susceptible lines of Bactrocera dorsalis and discovered a CBE BdE5 in the naled-resistant line with remarkable quantitative elevation in isoenzyme patterns of esterase activity, which was also inhibited by OPs such as paraoxon and naled. Correlation was found between naled resistance and flies with a higher percentage of intensive BdE5 bands. The current study used in vivo inhibition assays to show that, under diagnostic doses of naled, overall extent of inhibition on CBEs was much greater in the susceptible line than in the naled-resistant line. Through NanoLC-nanoESi-MS/MS analysis, BdE5 was identified and annotated as an esterase FE4-like (XP_011200445.1). Rapid amplification of cDNA ends was used to obtain the full-length 2,012-bp BdE5 cDNA, which contained an open reading frame of 1,770 bp and encoded a putative protein of 590 amino acid residues. Phylogenetic analysis revealed that BdE5 is a secreted β esterases (E clade) closely related to CG6414 (NP_570089), a CBE in Drosophila melanogaster. As insect CBE genes belong to a large multigene carboxyl/cholinesterases (CCE) family, many CBEs related to OP resistance have not yet been found and their specific roles have yet to be studied. To discover more OP resistance-related CBEs, in this study, we used RNA-seq analysis to obtain transcript RPKM ratios of five OP-resistant lines to one susceptible line, screening out CCE genes. Six genes had ratios of more than two and were chosen as candidate genes from a total of 33 genes. RT-qPCR was used to check the relative expression levels of candidate genes in the naled-, trichlorfon-, malathion-resistant lines compared to the susceptible line, and three of these candidate genes were thought to be related to OP resistance due to their significantly higher (or more than two-fold) expression levels in the three resistant lines. BdE5 was one of these genes and its transcript level result was consistent with previous findings of esterase patterns and activity assays. The gene with the highest relative expression level was annotated as carboxylesterase B2 (AGU42836.1)(orthologous gene of alpha-Esterase-7, αE7), and was successfully used to express recombinant αE7 with CBE catalytic activity in Spodoptera frugiperda Sf21 cells using the baculovirus expression system. The results in this study provide further understanding on how CBEs relate to OP resistance, and the B. dorsalis recombinant αE7 provides a basis for future studies of resistance mechanisms or development of related technical applications. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:32:43Z (GMT). No. of bitstreams: 1 ntu-106-R01632017-1.pdf: 3501516 bytes, checksum: 144384f381eadc6522e87f7bf73d587b (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 ii ABSTRACT iv 目錄 vi 表次 ix 圖次 x 附錄次 xi 壹、前言 1 貳、往昔研究 4 2.1 東方果實蠅之生物學 4 2.2 有機磷殺蟲劑 5 2.3 昆蟲對殺蟲劑之抗藥性機制 5 2.4 羧酸酯酶 6 2.4.1 羧酸酯酶之功能、分類與結構 6 2.4.2 羧酸酯酶之催化機制與抑制反應 8 2.4.3 羧酸酯酶之有機磷抗藥性研究 9 2.4.4 羧酸酯酶重組蛋白之表現 10 2.5 次世代定序 11 2.5.1 次世代定序技術之簡介 11 2.5.2 次世代定序於東方果實蠅抗藥性研究之應用 12 參、材料與方法 13 3.1 藥品 13 3.2 蟲源與抗性篩選 13 3.3 生物檢定 14 3.4 酯酶萃取 14 3.5 蛋白質定量 14 3.6 原態膠體電泳與酯酶活性染色 15 3.7 十二烷基硫酸鈉聚丙烯醯胺凝膠電泳 15 3.8 蛋白質染色 15 3.8.1 Coomassie Brilliant Blue R-250 15 3.8.2 SYPRO® Ruby 16 3.8.3 銀染法 16 3.9 乃力松之酯酶體內抑制試驗 16 3.10 質譜分析 16 3.11 BdE5 cDNA 全長定序 17 3.12 親緣分析 18 3.13 RNA-seq 分析 18 3.13.1 東方果實蠅羧酸酯酶相關基因之分類 18 3.13.2 RPKM ratio 計算 19 3.14 羧酸酯酶基因表現量分析 19 3.15 表現載體之構築與轉染 20 3.15.1 構築中間載體 20 3.15.2 構築穿梭載體 20 3.15.3 轉染穿梭載體 21 3.15.4 病毒力價測定 22 3.16 αE7重組蛋白之表現與純化 22 3.17 西方墨點法 24 3.18 αE7重組蛋白之酯酶活性測定 24 3.19 統計方法 25 肆、結果 26 4.1 有機磷抗性品系之抗性程度 26 4.2 體內抑制試驗 26 4.3 乃力松抗性品系 BdE5 之蛋白質身分鑑定 26 4.4 BdE5 cDNA 之序列分析及親緣分析 27 4.5 東方果實蠅之羧酸/膽鹼酯酶 (CCE) 之基因註解與分類 27 4.6 以 RPKM ratio 挑選候選基因之結果與分析 28 4.7 有機磷抗感性品系之候選基因表現量差異 28 4.8 載體之建立與轉染 28 4.9 病毒貯存溶液之感染力價與病毒濃度 29 4.10 αE7 重組蛋白之表現與純化結果 29 4.11 αE7 重組蛋白之酯酶活性 30 伍、討論 32 陸、參考文獻 64 柒、附錄 80 | |
dc.language.iso | zh-TW | |
dc.title | 東方果實蠅與有機磷抗藥性相關之羧酸酯酶研究 | zh_TW |
dc.title | A study of carboxylesterases associated with organophosphate resistance in Bactrocera dorsalis | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 戴淑美,張誠,馮海東,莊榮輝 | |
dc.subject.keyword | 東方果實蠅,有機磷抗藥性,羧酸酯?,乃力松,昆蟲桿狀病毒, | zh_TW |
dc.subject.keyword | Bactrocera dorsalis,organophosphate resistance,carboxylesterases,naled,baculovirus, | en |
dc.relation.page | 87 | |
dc.identifier.doi | 10.6342/NTU201700531 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-02-15 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
顯示於系所單位: | 昆蟲學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-1.pdf 目前未授權公開取用 | 3.42 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。