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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王俊能(Chun-Neng Wang) | |
dc.contributor.author | Hsin-Yi Huang | en |
dc.contributor.author | 黃馨儀 | zh_TW |
dc.date.accessioned | 2021-06-16T09:31:46Z | - |
dc.date.available | 2022-02-17 | |
dc.date.copyright | 2017-02-17 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-02-15 | |
dc.identifier.citation | Arendt, J.D. (1997). Adaptive intrinsic growth rates: an integration across taxa.
The Quarterly Review of Biology 72, 149-177. Asbe, A., Matsushita, S.C., Gordon, S., Kirkpatrick, H., and Madlung, A. (2015). Floral reversion in Arabidopsis suecica is correlated with the onset of flowering and meristem transitioning. PloS one 10, e0127897. Ballerini, E.S., and Kramer, E.M. (2011). In the light of evolution: a reevaluation of conservation in the CO–FT regulon and its role in photoperiodic regulation of flowering time. Frontiers in plant science 2, 81. Battey, N.H., and Lyndon, R.F. (1986). Apical growth and modification of the development of primordia during re-flowering of reverted plants of Impatiens balsamina L. Annals of botany 58, 333-341. Battey, N.H., and Lyndon, R.F. (1990). Reversion of flowering. The Botanical Review 56, 162-189. Battey, N.H., and Tooke, F. (2002). Molecular control and variation in the floral transition. Current opinion in plant biology 5, 62-68. Bazzaz, F. (1979). The physiological ecology of plant succession. Annual review of Ecology & Systematics 10, 351-371. Bernier, G., Havelange, A., Houssa, C., Petitjean, A., and Lejeune, P. (1993). Physiological signals that induce flowering. The Plant Cell 5, 1147. Blackman, B.K., Strasburg, J.L., Raduski, A.R., Michaels, S.D., and Rieseberg, L.H. (2010). The role of recently derived FT paralogs in sunflower domestication. Current Biology 20, 629-635. Böhlenius, H., Huang, T., Charbonnel-Campaa, L., Brunner, A.M., Jansson, S., Strauss, S.H., and Nilsson, O. (2006). CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees. Science 312, 1040-1043. Bolouri Moghaddam, M.R., and Van den Ende, W. (2013). Sugars, the clock and transition to flowering. Frontiers in plant science 4, 22. Cataldi, T.R.I., Campa, C., and De Benedetto, G.E. (2000). Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection: The potential is still growing. Fresenius' Journal of Analytical Chemistry 368, 739-758. Cerdán, P.D., and Chory, J. (2003). Regulation of flowering time by light quality. Nature 423, 881-885. Chang, J. (2010). Study of inflorescence identity and floral determinacy genes on Inflorescence Transition in Titanotrichum. Master thesis. In Institute of Ecology & Evolutionary Biology (National Taiwan University) pp. 1-122. Chen, M., MacGregor, D.R., Dave, A., Florance, H., Moore, K., Paszkiewicz, K., Smirnoff, N., Graham, I.A., and Penfield, S. (2014). Maternal temperature history activates FLOWERING LOCUS T in fruits to control progeny dormancy according to time of year. Proc Natl Acad Sci U S A 111, 18787-18792. Chen, Y.J. (2009). Candidate genes analysis of flower reversion to bulbils in Titanotrichum oldhamii (Hemsl.) Soler. Master thesis. In Institute of Ecology & Evolutionary Biology (National Taiwan University), pp. 1-49. Clough, S.J., and Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium‐mediated transformation of Arabidopsis thaliana. The plant journal 16, 735-743. Coelho, C.P., Minow, M.A., Chalfun-Junior, A., and Colasanti, J. (2014). Putative sugarcane FT/TFL1 genes delay flowering time and alter reproductive architecture in Arabidopsis. Front Plant Science 5, 221. Corbesier, L., Lejeune, P., and Bernier, G. (1998). The role of carbohydrates in the induction of flowering in Arabidopsis thaliana: comparison between the wild type and a starchless mutant. Planta 206, 131-137. Deng, T., Kim, C., Zhang, D.-G., Zhang, J.-W., Li, Z.-M., Nie, Z.-L., and Sun, H. (2013). Zhengyia shennongensis: A new bulbiliferous genus and species of the nettle family (Urticaceae) from central China exhibiting parallel evolution of the bulbil trait. Taxon 62, 89-99. Elmqvist, T., and Cox, P.A. (1996). The evolution of vivipary in flowering plants. Oikos 77, 3. Flagel, L.E., and Wendel, J.F. (2009). Gene duplication and evolutionary novelty in plants. New Phytologist 183, 557-564. Frohman, M.A., Dush, M.K., and Martin, G.R. (1988). Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proceedings of the National Academy of Sciences 85, 8998-9002. Genty, B., Briantais, J.-M., and Baker, N.R. (1989). The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA) - General Subjects 990, 87-92. Gibson, S.I. (2005). Control of plant development and gene expression by sugar signaling. Current opinion in plant biology 8, 93-102. Goetz, M., Godt, D.E., Guivarc'h, A., Kahmann, U., Chriqui, D., and Roitsch, T. (2001). Induction of male sterility in plants by metabolic engineering of the carbohydrate supply. Proc Natl Acad Sci U S A 98, 6522-6527. Golembeski, G.S., and Imaizumi, T. (2015). Photoperiodic regulation of florigen function in Arabidopsis thaliana. Arabidopsis Book 13, e0178. Graf, A., Schlereth, A., Stitt, M., and Smith, A.M. (2010). Circadian control of carbohydrate availability for growth in Arabidopsis plants at night. Proc Natl Acad Sci U S A 107, 9458-9463. Guignard, C., Jouve, L., Bogéat-Triboulot, M.B., Dreyer, E., Hausman, J.-F., and Hoffmann, L. (2005). Analysis of carbohydrates in plants by high-performance anion-exchange chromatography coupled with electrospray mass spectrometry. Journal of Chromatography A 1085, 137-142. Harig, L., Beinecke, F.A., Oltmanns, J., Muth, J., Muller, O., Ruping, B., Twyman, R.M., Fischer, R., Prufer, D., and Noll, G.A. (2012). Proteins from the FLOWERING LOCUS T-like subclade of the PEBP family act antagonistically to regulate floral initiation in tobacco. Plant Journal 72, 908-921. Hiraoka, K., Yamaguchi, A., Abe, M., and Araki, T. (2013). The florigen genes FT and TSF modulate lateral shoot outgrowth in Arabidopsis thaliana. Plant Cell Physiology 54, 352-368. Ho, Y.W. (2016). Duplication of WUSCHEL and AGAMOUS involvedin the bulbil development of Titanotrichum. Master thesis. In Institute of Life Science (National Taiwan University), pp. 1-111. Hsu, C.Y., Adams, J.P., Kim, H., No, K., Ma, C., Strauss, S.H., Drnevich, J., Vandervelde, L., Ellis, J.D., Rice, B.M. (2011). FLOWERING LOCUS T duplication coordinates reproductive and vegetative growth in perennial poplar. Proc Natl Acad Sci U S A 108, 10756-10761. Hsu, C.Y., Liu, Y., Luthe, D.S., and Yuceer, C. (2006). Poplar FT2 shortens the juvenile phase and promotes seasonal flowering. Plant Cell 18, 1846-1861. Kardailsky, I., Shukla, V.K., Ahn, J.H., Dagenais, N., Christensen, S.K., Nguyen, J.T., Chory, J., Harrison, M.J., and Weigel, D. (1999). Activation tagging of the floral inducer FT. Science 286, 1962-1965. King, R.W., Hisamatsu, T., Goldschmidt, E.E., and Blundell, C. (2008). The nature of floral signals in Arabidopsis. I. Photosynthesis and a far-red photoresponse independently regulate flowering by increasing expression of FLOWERING LOCUS T (FT). Journal of Experimental Botany 59, 3811-3820. Kinmonth-Schultz, H.A., Golembeski, G.S., and Imaizumi, T. (2013). Circadian clock-regulated physiological outputs: dynamic responses in nature. Seminars in Cell & Developmental Biology 24, 407-413. Kinoshita, T., Ono, N., Hayashi, Y., Morimoto, S., Nakamura, S., Soda, M., Kato, Y., Ohnishi, M., Nakano, T., Inoue, S., et al. (2011). FLOWERING LOCUS T regulates stomatal opening. Current Biology 21, 1232-1238. Kobayashi, Y., Kaya, H., Goto, K., Iwabuchi, M., and Araki, T. (1999). A pair of related genes with antagonistic roles in mediating flowering signals. Science 286, 1960-1962. Koch, K. (2004). Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Current opinion in plant biology 7, 235-246. Kotoda, N., Hayashi, H., Suzuki, M., Igarashi, M., Hatsuyama, Y., Kidou, S., Igasaki, T., Nishiguchi, M., Yano, K., Shimizu, T., et al. (2010). Molecular characterization of FLOWERING LOCUS T-like genes of apple (Malus x domestica Borkh.). Plant Cell Physiology 51, 561-575. Kozłowski, J. (1992). Optimal allocation of resources to growth and reproduction: Implications for age and size at maturity. Trends in Ecology & Evolution 7, 15-19. Krzymuski, M., Andres, F., Cagnola, J.I., Jang, S., Yanovsky, M.J., Coupland, G., and Casal, J.J. (2015). The dynamics of FLOWERING LOCUS T expression encodes long-day information. Plant Journal 83, 952-961. Lastdrager, J., Hanson, J., and Smeekens, S. (2014). Sugar signals and the control of plant growth and development. Journal of Experimental Botany 65, 799-807. Lauxmann, M.A., Annunziata, M.G., Brunoud, G., Wahl, V., Koczut, A., Burgos, A., Olas, J.J., Maximova, E., Abel, C., Schlereth, A., et al. (2016). Reproductive failure in Arabidopsis thaliana under transient carbohydrate limitation: flowers and very young siliques are jettisoned and the meristem is maintained to allow successful resumption of reproductive growth. Plant Cell & Environment 39, 745-767. Lee, R., Baldwin, S., Kenel, F., McCallum, J., and Macknight, R. (2013). FLOWERING LOCUS T genes control onion bulb formation and flowering. Nature Communications 4, 2884. Liu, L., Farrona, S., Klemme, S., and Turck, F.K. (2014). Post-fertilization expression of FLOWERING LOCUS T suppresses reproductive reversion. Front Plant Science 5, 164. Liu, Y.C., Liu, C.H., Lin, Y.C., Lu, C.H., Chen, W.H., and Wang, H.L. (2015). Effect of low irradiance on the photosynthetic performance and spiking of Phalaenopsis. Photosynthetica 54, 259-266. Mandel, M.A., Gustafson-Brown, C., Savidge, B., and Yanofsky, M.F. (1992). Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Matsoukas, I.G., Massiah, A.J., and Thomas, B. (2013). Starch metabolism and antiflorigenic signals modulate the juvenile-to-adult phase transition in Arabidopsis. Plant Cell & Environment 36, 1802-1811. Maxwell, K., and Johnson, G.N. (2000). Chlorophyll fluorescence - a practical guide. Journal of Experimental Botany 51, 659-668. McCullough, E., Wright, K.M., Alvarez, A., Clark, C.P., Rickoll, W.L., and Madlung, A. (2010). Photoperiod‐dependent floral reversion in the natural allopolyploid Arabidopsis suecica. New Phytologist 186, 239-250. Michaels, S.D., Himelblau, E., Kim, S.Y., Schomburg, F.M., and Amasino, R.M. (2005). Integration of flowering signals in winter-annual Arabidopsis. Plant Physiology 137, 149-156. Müller-Xing, R., Clarenz, O., Pokorny, L., Goodrich, J., and Schubert, D. (2014). Polycomb-group proteins and flowering LOCUS T maintain commitment to flowering in Arabidopsis thaliana. Plant Cell 26, 2457-2471. Navarro, C., Abelenda, J.A., Cruz-Oro, E., Cuellar, C.A., Tamaki, S., Silva, J., Shimamoto, K., and Prat, S. (2011). Control of flowering and storage organ formation in potato by FLOWERING LOCUS T. Nature 478, 119-122. Patrick, J.W., and Colyvas, K. (2014). Crop yield components – photoassimilate supply- or utilisation limited-organ development? Functional Plant Biology 41, 893. Pin, P.A., Benlloch, R., Bonnet, D., Wremerth-Weich, E., Kraft, T., Gielen, J.J., and Nilsson, O. (2010). An antagonistic pair of FT homologs mediates the control of flowering time in sugar beet. Science 330, 1397-1400. Pin, P.A., and Nilsson, O. (2012). The multifaceted roles of FLOWERING LOCUS T in plant development. Plant Cell & Environment 35, 1742-1755. Ratter, J. (1963). Some chromosome numbers in the Gesneriaceae. Notes Roy Bot Gard Edinburgh 24, 221-229. Ritchie, G.A. (2006). Chlorophyll fluorescence: what is it and what do the numbers mean? USDA Forest Service Proceedings, 35-43. Rogers, S.O., and Bendich, A.J. (1985). Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Molecular Biology 5, 69-76. Roldán, M., Gómez‐Mena, C., Ruiz‐García, L., Salinas, J., and Martínez‐Zapater, J.M. (1999). Sucrose availability on the aerial part of the plant promotes morphogenesis and flowering of Arabidopsis in the dark. The Plant Journal 20, 581-590. Rolland, F., Baena-Gonzalez, E., and Sheen, J. (2006). Sugar sensing and signaling in plants: conserved and novel mechanisms. Annual Review of Plant Biology 57, 675-709. Sadras, V.O., and Denison, R.F. (2009). Do plant parts compete for resources? An evolutionary viewpoint. New Phytologist 183, 565-574. Scialdone, A., Mugford, S.T., Feike, D., Skeffington, A., Borrill, P., Graf, A., Smith, A.M., and Howard, M. (2013). Arabidopsis plants perform arithmetic division to prevent starvation at night. Elife 2, e00669. Shirley, H.L. (1929). The influence of light intensity and light quality upon the growth of plants. American Journal of Botany, 354-390. Simon, R., Igeño, M.I., and Coupland, G. (1996). Activation of floral meristem identity genes in Arabidopsis. Nature 384, 59. Song, Y.H., Ito, S., and Imaizumi, T. (2010). Similarities in the circadian clock and photoperiodism in plants. Current opinion in plant biology 13, 594-603. Stitt, M., and Zeeman, S.C. (2012). Starch turnover: pathways, regulation and role in growth. Current opinion in plant biology 15, 282-292. Takada, S., and Goto, K. (2003). TERMINAL FLOWER2, an Arabidopsis homolog of HETEROCHROMATIN PROTEIN1, counteracts the activation of FLOWERING LOCUS T by CONSTANS in the vascular tissues of leaves to regulate flowering time. Plant Cell 15, 2856-2865. Thornley, J. (1998). Dynamic model of leaf photosynthesis with acclimation to light and nitrogen. Annals of Botany 81, 421-430. Tooke, F., and Battey, N. (2003). Models of shoot apical meristem function. New Phytologist 159, 37-52. Tooke, F., Ordidge, M., Chiurugwi, T., and Battey, N. (2005). Mechanisms and function of flower and inflorescence reversion. Journal of Experimental Botany 56, 2587-2599. Wahl, V., Ponnu, J., Schlereth, A., Arrivault, S., Langenecker, T., Franke, A., Feil, R., Lunn, J.E., Stitt, M., and Schmid, M. (2013). Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thaliana. Science 339, 704-707. Wang, C.N., and Cronk, Q.C.B. (2003). Meristem fate and bulbil formation in Titanotrichum (Gesneriaceae). American Journal of Botany 90, 1696-1707. Wang, C.N., Möller, M., and Cronk, Q.C.B. (2004). Altered expression of GFLO, the Gesneriaceae homologue of FLORICAULA / LEAFY, is associated with the transition to bulbil formation in Titanotrichum oldhamii. Development Genes & Evolution 214, 122-127. Wang, C.N., Möller, M., and Cronk, Q.C.B. (2004). Aspects of sexual failure in the reproductive processes of a rare bulbiliferous plant, Titanotrichum oldhamii (Gesneriaceae), in subtropical Asia. Sexual Plant Reproduction 17, 23-31. Wang, C.N., Möller, M., and Cronk, Q.C.B. (2004). Phylogenetic Position of Titanotrichum oldhamii (Gesneriaceae) Inferred from four different gene regions. Systematic Botany 29, 407-418. Weigel, D., Alvarez, J., Smyth, D.R., Yanofsky, M.F., and Meyerowitz, E.M. (1992). LEAFY controls floral meristem identity in Arabidopsis. Cell 69, 843-859. Wickland, D.P., and Hanzawa, Y. (2015). The FLOWERING LOCUS T/TERMINAL FLOWER 1 Gene Family: Functional Evolution and Molecular Mechanisms. Molecular Plant 8, 983-997. Wigge, P.A., Kim, M.C., Jaeger, K.E., Busch, W., Schmid, M., Lohmann, J.U., and Weigel, D. (2005). Integration of spatial and temporal information during floral induction in Arabidopsis. Science 309, 1056-1059. Winter, H., and Huber, S.C. (2000). Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes. Critical Reviews in Biochemistry & Molecular Biology 35, 253-289. Winterhagen, P., Tiyayon, P., Samach, A., Hegele, M., and Wunsche, J.N. (2013). Isolation and characterization of FLOWERING LOCUS T subforms and APETALA1 of the subtropical fruit tree Dimocarpus longan. Plant Physiology & Biochemistry 71, 184-190. Wu, P.A. (2016). Function of CENTRORADIALIS in Regulating Floral Reversion of Titanotrichum. Master thesis. In Institute of Ecology & Evolutionary Biology (National Taiwan University), pp. 1-112. Wu, P.H., Liu, C.H., Tseng, K.M., Liu, Y.C., Chen, C.C., Yang, P.P., Huang, Y.X., Chen, W.H., and Wang, H.L. (2013). Low irradiance alters carbon metabolism and delays flower stalk development in two orchids. Biologia Plantarum 57, 764-768. Yamaguchi, A., Kobayashi, Y., Goto, K., Abe, M., and Araki, T. (2005). TWIN SISTER OF FT (TSF) acts as a floral pathway integrator redundantly with FT. Plant Cell Physiology 46, 1175-1189. Yoo, S.K., Chung, K.S., Kim, J., Lee, J.H., Hong, S.M., Yoo, S.J., Yoo, S.Y., Lee, J.S., and Ahn, J.H. (2005). CONSTANS activates SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 through FLOWERING LOCUS T to promote flowering in Arabidopsis. Plant physiology 139, 770-778. Yu, S., Lian, H., and Wang, J.W. (2015). Plant developmental transitions: the role of microRNAs and sugars. Current opinion in plant biology 27, 1-7. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59655 | - |
dc.description.abstract | 營養期至繁殖期之時期轉換受嚴密的調控,環境訊息為其中一個重要的因子。俄氏草為兼行有性生殖與無性生殖的珠芽植物,夏季時開花(有性生殖),秋季日照縮短時,其花序頂端之原基會反轉成珠芽序(變形花序),而原本花序上的花原基逆轉為由珠芽(無性生殖)組成的珠芽叢構造,稱為成花反轉。此外,俄氏草在原生環境條件中,若該個體生長於陰暗或是養分不充足條件下,俄氏草植株發育成熟進入繁殖時期,植株將不經歷有性生殖開花,莖頂將直接轉換發育為珠芽序。故弱光可能會誘導成花反轉。為了調查弱光(醣類受限)條件下是否促使成花反轉發生,進入繁殖期植物以正常光照(100 至 150 μmol m-2s-1)和弱光(5 至15 μmol m-2s-1)處理做為比較。弱光處理將造成花朵敗育,產生的敗育花可視為成花反轉之過渡產物。另外,持續正常光處理組別之花序最終仍發生成花反轉,也暗示著醣類限制可能會促使成花反轉。再者,進一步測量俄氏草花序發育時期醣含量較與珠芽時期為高。因此,藉由遮光處理模擬醣限制情況加以驗證。將第一朵花開時期之植株遮光90%葉面積(S90),並記錄花序上節位狀態至轉換發育珠芽序時期以評估資源限制的效應。於S90個體約10.5天達百分之五十開花,早於未遮光之對照組的13天。並且S90組於轉換發育珠芽期前約有25朵花(67個節位)較對照組32朵花(74個節位)少。這些結果支持在藉由遮光處理導致的醣限制條件下,花期受到限制而增加轉換珠芽發育的可能性。
另一方面,為了調查光週期對俄氏草時期轉換的效應,自珠芽開始分別培養於長日照(LDs, 8D: 16L)或短日照條件(SDs, 16D: 8L),其中短日照植株進入繁殖期時將不發育花而直接轉換發育珠芽,而長日照條件下將誘導更多花發育。因此,光週期調控俄氏草繁殖時期之時期轉換。FLOWERING LOCUS T (FT)是匯整光週期訊息調控開花重要因子,而俄氏草之FT同源基因可能與成花反轉或珠芽發育現象具關連性。俄氏草的2條ToFT genes皆受光週期調控,於長日照條件表現呈週期震盪(光照後16 hr時間點表現量較4hr高),短日照下兩個時間點(光照後0 hr和8 hr兩個時間點)表現量差異較長日照小。此外,ToFT genes皆於轉換珠芽時期大量表現,且ToFT1於莖頂表現量較ToFT2高。藉由將俄氏草ToFT genes至阿拉伯芥全株過量表現,皆顯示2條ToFT genes為提早開花之性狀,顯示其功能保守性。僅管2條ToFT genes為功能性重覆,但已逐漸出現分化情形。 | zh_TW |
dc.description.abstract | The transition from vegetative to reproductive phase was strictly regulated: environmental signal is one of the important factors. Titanotrichum oldhamii utilizes a mixed sexual and asexual reproductive strategy, which is flowering in summer and generating bulbils to replace flowers on inflorescences, which called floral reversion, when day length shortens. In addition, it generates bulbils directly under weak light or nutritional inadequacy condition. To investigate whether light intensity and carbohydrate limitation condition promote floral reversion, pre-flowering plants were treated with normal (100 to 150 μmol m-2s-1) and weak (5 to 15 μmol m-2s-1) light intensity respectively. The weak light treatment led to aborted flowers, an intermediate form of floral reversion in T. oldhamii. Besides, the continuous normal light treatment ones, which inflorescence apex eventually transit to bulbil development. Moreover, the sugar content of transit to bulbil stage is lower than inflorescences stage. Those implies carbohydrate limitation might restrict flowering and trigger reversion to bulbils. Shading trial was proposed to mimic a carbohydrate limitation condition. Plants bloomed on the first day were shaded 90% leaf area and were recorded number of flower on inflorescence nodes generated before reversion to bulbils. Day to 50% flowering for shaded individuals (around 10.5 days) was earlier than non-shaded ones (around 13 days) and also number of fully developed flowers is less on shaded individuals (about 25 flowers from 67 nodes) before reversion compared to non-shaded control (about 32 flowers from 74 nodes). This supported that carbohydrate limitation restricts flowering and increases possibility of transition to bulbils.
On the other hands, to investigate the effect of photoperiod on developmental switches of inflorescence transition, seedlings were grown under long days (LDs, 8D: 16L) or short days (SDs, 16D: 8L) photoperiod. SDs induced the inflorescence of plants transiting to bulbiliferous shoots directly without normal flowers, in contrast, LDs induced more flowers. FLOWERING LOCUS T (FT), a pivotal flowering pathway integrator for photoperiod signals, could possibly be involved in floral reversion to bulbils. Two ToFT genes are photoperiod-dependent with circadian oscillation expressed in LDs (expression levels are higher at 16 hr than 4hr after dawn), but the pattern is unclear in SDs (compare 0 hr and 8 hr after dawn). Moreover, ToFT genes had higher expression during transition stage to bulbil, and also ToFT1 epxerssion is higher than ToFT2 at apex in this period. In addition, functional studies via ectopically expressing ToFT genes in Arabidopsis can promote early flowering phenotype, which reveals a conserve function of two ToFT genes. Although two ToFT genes would be functional redundant, they might have sub-functionalization. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:31:46Z (GMT). No. of bitstreams: 1 ntu-106-R03b42014-1.pdf: 8054748 bytes, checksum: 934c7e8fc9d923ca2d3f3de305146811 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 中文摘要…………………………………………………….……………………….. I
英文摘要…………………….……………………...…….……….…………………III 目錄…………………….……………………...………...……….………………...…V 圖目錄…………………….……………….....………...……….………………...….IX 表目錄…………………….……………………...………...……….………………..XI 第一章、前言………………………………………..…………………….………….1 一、俄氏草Titanotrichum oldhamii簡介………...………………….………...1 二、時期轉換(phase transiton)與成花反轉(floral reversion)…...….…..….….…..3 (一)、開花途徑回顧………………………..……………….…………………..3 (二)、成花反轉(floral reversion)現象與機制回顧……………………………..4 (三)、光合狀態(photosynthetic status)與醣類供應對繁殖時期調控…...……..6 三、FLOWERING LOCUS T, FT具多樣功能角色..……………….………….….8 四、俄氏草成花反轉分子發育機制與過去研究概況…………….…………….10 五、研究目標…………………………………..…………………….…….……..11 第二章、材料方法…………………………………………………….…………….12 一、植物材料來源栽培…………………………………………….…………….12 二、光週期對時期轉換及成花反轉影響……………………….………….……12 (一)、光週期對俄氏草時期轉換及成花反轉調控………….………………..12 (二)、測試感知光週期之組織是否為葉片……………………….…………..12 三、光照條件改變養分狀態對成花反轉影響……………………….………….13 (一)、強弱光度與強弱光間隔期間處理………..…………………………….13 (二)、俄氏草成花反轉過程之醣含量變化……..…………………….………15 (三)、葉片遮光製造之養分供應受限條件是否促進成花反轉…...…..……..15 四、生理參數測量……………………………………………….……….………16 (一)、葉綠素螢光……………………………………………….……………..16 (二)、葉面積……………………………………………………..…………….17 (三)、鮮重與乾重…………………………………………..………………….17 (四)、醣類萃取與醣含量測量…………………………………..…………….17 五、基本分生方法……………………………………………….……….………19 (一)、DNA萃取…………………………………………….…………….……19 (二)、RNA萃取……………………………………………………….……….19 (三)、反轉錄cDNA………………………………………………….………...20 (四)、聚合酵素連鎖反應 (polymerase chain reaction, PCR) …….………….20 六、同源基因分離…….…………….…………….…………….……………..…21 (一)、Thermal asymmetric interlaced PCR, TAIL-PCR……………………….21 (二)、Rapid amplification of 3’ cDNA ends, 3’ RACE……………….………..23 (三)、Cloning and Clones identification……………….………………………25 七、親緣分析……………………………………….………………………….…25 八、反轉錄聚合酵素連鎖反應(RT-PCR)基因表現分析………………………..25 九、阿拉伯芥全株過量表現俄氏草目標基因…………………………………..26 (一)、載體構建……………………….………………………………………..26 (二)、農桿菌勝任細胞製備與轉型…………………………………………...27 (三)、花序沾黏法Floral dipping……………………………………………...28 (四)、種子篩選……………………………………………...............................28 十、統計方法……………………………………………............................29 第三章、結果…………………………………………………….………………….30 一、光週期對俄氏草時期轉換調控………………………….………………….30 二、強弱光度與強弱光間隔期間處理對於俄氏草花序發育狀態調控………..32 三、俄氏草成花反轉過程之醣含量變化與該現象關連性……….…………….35 (一)、成花反轉後之珠芽期醣含量減少…………………….………………..35 (二)、成花反轉過程生理參數變化…………………………………………...38 四、遮光處理對俄氏草成花反轉影響………………………………………......41 (一)、PSII光化學反應與醣含量……………………………...........................43 (二)、生理狀態…………………………….........................................48 五、俄氏草FLOWERING LOCUS T同源基因角色與時期轉換的關連性........51 (一)、親源關係分析………………………............................................51 (二)、ToFT genes於不同組織部位、長短日照及成花反轉與珠芽發育時期 表現模式分析……………….............................................54 (三)、阿拉伯芥全株過量表現之功能分析.....................................................61 第四章、討論………………………..................63 一、光週期對俄氏草時期轉換調控─長日照開花而短日照發育珠芽..............63 二、強弱光度與強弱光間隔期間處理對於俄氏草花序發育狀態影響..............64 (一)、弱光處理可能造成養分短缺使花期受限產生成花反轉過渡產物.......64 (二)、敗育花與珠芽序間隔發育顯示花器與珠芽發育之轉換具可逆性.......64 (三)、弱光處理造成個體死亡的可能原因以及實驗限制...............................66 三、俄氏草成花反轉時期與遮光處理之資源分配及生理狀態..........................67 (一)、俄氏草於繁殖時期發育花序與地下根莖,於養分受限條件下將縮減於花序(繁殖組織)及地下根莖(貯藏養分組織)資源.............................67 (二)、遮光處理後對受光葉片生理狀態改變的其他變因...............................67 四、俄氏草於轉換發育珠芽序時期醣含量供應的減少可能促使成花反轉......69 (一)、成花反轉後的珠芽期醣含量減少...................................................69 (二)、遮光處理減少醣含量使俄氏草花期發育受限以促使成花反轉...........70 (三)、遮光實驗限制與改進方向.....................................................70 五、俄氏草FLOWERING LOCUS T同源基因角色與時期轉換的關連性─ ToFT1與ToFT2可能為功能重複(functional redundant)而逐漸分化 (subfunctionalization) .............................72 (一)、ToFT1與ToFT2表現模式相仿而逐漸分化(sub-functionalization) ….72 (二)、俄氏草ToFT1與ToFT2具有新功能參與珠芽發育的可能性…..........73 第五章、結論…………………..........................................75 第六章、參考文獻…………………..............................................77 附錄………………............................................87 | |
dc.language.iso | zh-TW | |
dc.title | 光週期與養分限制對俄氏草成花反轉及時期轉換的效應與ToFT genes參與角色 | zh_TW |
dc.title | Effects of photoperiod, resource limitation and the role of ToFT genes on floral reversion and phase transition in Titanotrichum oldhamii | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 鄭石通(Shih-Tong Jeng) | |
dc.contributor.oralexamcommittee | 陳仁治(Jen-Chih Chen),吳素幸(Shu-Hsing Wu),余天心(Tien-Shin Yu) | |
dc.subject.keyword | 俄氏草,珠芽,成花反轉,時期轉換,光週期,碳素資源,FLOWERING LOCUS T, | zh_TW |
dc.subject.keyword | Titanotrichum oldhamii,bulbils,floral reversion,phase transiton,photoperiod,carbon resource,FLOWERING LOCUS T, | en |
dc.relation.page | 94 | |
dc.identifier.doi | 10.6342/NTU201700635 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-02-15 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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