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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳逸聰(Yit-Tsong Chen) | |
| dc.contributor.author | Hsu-Cheng Chiang | en |
| dc.contributor.author | 江旭正 | zh_TW |
| dc.date.accessioned | 2021-06-16T09:29:05Z | - |
| dc.date.available | 2020-06-12 | |
| dc.date.copyright | 2017-06-12 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-03-28 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59589 | - |
| dc.description.abstract | 阿茲海默症(Alzheimer’s disease, AD)是最常見的神經退化性疾病之一，主要病理特徵為神經細胞外的乙型類澱粉蛋白斑塊(β-amyloid plaque)，斑塊中主要成分乙型類澱粉蛋白(β-amyloid, Aβ)是一段由40到42個胺基酸組合成的胜肽(Aβ40, Aβ42)。阿茲海默症的真正成因至今未明，也沒有治療的藥物，因此乙型類澱粉蛋白是阿茲海默症的研究焦點之一。本研究利用單層石墨烯(graphene)製成場效電晶體生物感測器(field-effect transistor biosensor)來研究乙型類澱粉蛋白單體在脂雙層膜上的聚集現象(aggregation)。
石墨烯的高化學穩定性、高載子遷移率、高導熱、高導電性、高機械強度、可撓性，以及高透光率，讓其應用受到許多領域的關注與研究，石墨烯的二維結構亦可以提供磷脂雙層穩定支撐，因此本研究以單層石墨烯在透明石英基板表面製成場效電晶體，接著在元件表面上以囊泡融合法(vesicle fusion method)鋪上含有神經節苷脂GM1之支撐性磷脂雙層(supported lipid bilayer, SLB)，並透過液相閘極(solution gate)量測到乙型類澱粉蛋白聚集現象，並使用Thioflavin T做為螢光探針驗證脂雙層膜上形成之纖維狀聚集。此「膜修飾石墨烯場效電晶體」提供了一個新的生物感測平台，未來可應用來進一步了解乙型類澱粉蛋白聚集機制、篩檢乙型類澱粉蛋白聚集抑制藥物。 | zh_TW |
| dc.description.abstract | Alzheimer’s disease (AD) is the most common neurodegenerative disease and is characterized by neurofibrillary tangles and amyloid plaques. As the main component of the amyloid plagues, amyloid beta (Aβ), a peptide consisting of 40-42 amino acids, are crucially involved in Alzheimer’s disease. However, the knowledge around the disease pathogenesis is poorly understood and there is no effective cure for AD. Hence, a great deal of attention has focused on the study of Aβ. This research uses a lipid-modified graphene-based field-effect transistor (G-FET) as a biosensor to study the aggregation of Aβ on the supported lipid bilayer.
Graphene has shown a great potential for research and applications because of its high chemical stability, high carrier mobility, high thermal conduction, high conductivity, flexibility, and high transmittance. Due to its two-dimensional (2D) nature, graphene possesses a large and stable interface with lipid membranes. We fabricated G-FET on a transparent quartz substrate, then modified a supported lipid bilayer containing GM1 on the G-FET by the vesicle fusion method. The as-fabricated G-FET biosensor is able to detect the aggregation of Aβ on the GM1 containing SLB. This aggregation is also verified by using Thioflavin T as a fluorescence indicator. With the capability of sensitively detecting the Aβ aggregation, this lipid-modified G-FET can be employed as a highly sensitive biosensing platform for further investigation of the mechanism of Aβ aggregation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T09:29:05Z (GMT). No. of bitstreams: 1 ntu-106-R03223224-1.pdf: 7014683 bytes, checksum: 3dd6884d3cc92ea7f30fc62c805874b8 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 口試委員會審定書 ........................................................................................................... i
中文摘要 .......................................................................................................................... ii ABSTRACT .................................................................................................................... iii 目錄 ................................................................................................................................. iv 圖目錄 ............................................................................................................................. vi Chapter 1 導論............................................................................................................ 1 1.1 石墨烯簡介 .................................................................................................... 1 1.2 場效電晶體生物感測器 ................................................................................ 2 1.3 乙型類澱粉蛋白 ............................................................................................ 3 1.4 研究動機 ........................................................................................................ 3 Chapter 2 文獻回顧.................................................................................................... 5 2.1 石墨烯之結構 ................................................................................................ 5 2.2 石墨烯之製備 ................................................................................................ 8 2.2.1 機械剝離法(mechanical exfoliation) .................................................... 8 2.2.2 氧化石墨烯還原(reduction of graphene oxide) ................................... 9 2.2.3 碳化矽磊晶成長法(epitaxial growth) ................................................ 10 2.2.4 化學氣相沉積合成(chemical vapor deposition, CVD) ..................... 10 2.3 石墨烯檢測方法 .......................................................................................... 13 2.3.1 拉曼光譜(Raman spectroscopy) ......................................................... 13 2.3.2 光學散射 ............................................................................................. 16 2.4 石墨烯場效電晶體生物感測器 .................................................................. 18 2.5 乙型類澱粉蛋白(β-amyloid, Aβ) ................................................................ 21 2.5.1 乙型類澱粉蛋白簡介 ......................................................................... 21 2.5.2 乙型類澱粉蛋白與神經節苷脂GM1 ............................................... 23 2.6 支撐性脂雙層膜 .......................................................................................... 26 Chapter 3 材料與方法 ............................................................................................. 31 3.1 石墨烯場效電晶體元件製作 ...................................................................... 31 3.1.1 CVD合成石墨烯 ............................................................................... 31 3.1.2 以聚甲基丙烯酸甲酯轉置石墨烯 ..................................................... 32 3.1.3 熱蒸鍍金屬電極 ................................................................................. 34 3.1.4 微流道系統 ......................................................................................... 34 3.2 生物感測平台 .............................................................................................. 35 3.2.1 鋪放磷脂雙層 ..................................................................................... 35 3.2.2 量測裝置 ............................................................................................. 37 3.3 檢驗儀器 ...................................................................................................... 38 3.3.1 光學金相顯微鏡 ................................................................................. 38 3.3.2 原子力顯微鏡（atomic force microscope, AFM） ........................... 39 3.3.3 共軛焦顯微鏡 ..................................................................................... 40 3.3.4 拉曼光譜儀 ......................................................................................... 41 Chapter 4 結果與討論 ............................................................................................. 42 4.1 石墨烯品質鑑定 .......................................................................................... 42 4.2 支撐性磷脂雙層鑑定 .................................................................................. 43 4.2.1 脂囊泡製備 ......................................................................................... 43 4.2.2 原子力顯微鏡影像 ............................................................................. 45 4.2.3 光漂白螢光恢復 ................................................................................. 47 4.2.4 液相量測 ............................................................................................. 49 4.3 量測乙型類澱粉蛋白聚集 .......................................................................... 52 4.3.1 控制組實驗(control experiments) ...................................................... 52 4.3.2 液相量測 ............................................................................................. 53 4.3.3 ThT螢光檢測 ..................................................................................... 55 Chapter 5 結論.......................................................................................................... 57 參考文獻 ......................................................................................................................... 58 | |
| dc.language.iso | zh-TW | |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | 場效電晶體 | zh_TW |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | 支撐性磷脂雙層 | zh_TW |
| dc.subject | 場效電晶體 | zh_TW |
| dc.subject | 支撐性磷脂雙層 | zh_TW |
| dc.subject | 乙型類澱粉蛋白 | zh_TW |
| dc.subject | 石墨烯 | zh_TW |
| dc.subject | 乙型類澱粉蛋白 | zh_TW |
| dc.subject | 石墨烯 | zh_TW |
| dc.subject | supported lipid bilayer | en |
| dc.subject | Alzheimer’s disease | en |
| dc.subject | amyloid-beta | en |
| dc.subject | graphene | en |
| dc.subject | field-effect transistor | en |
| dc.subject | biosensor | en |
| dc.subject | supported lipid bilayer | en |
| dc.subject | Alzheimer’s disease | en |
| dc.subject | amyloid-beta | en |
| dc.subject | graphene | en |
| dc.subject | field-effect transistor | en |
| dc.subject | biosensor | en |
| dc.title | 以磷脂質修飾之石墨烯場效電晶體量測乙型類澱粉蛋白聚集 | zh_TW |
| dc.title | Using Lipid-Modified Graphene-Based Field-Effect Transistors to Detect Aggregation of Amyloid-Beta | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳巧貞(Chiao-Chen Chen),陳韻如(Yun-Ru Chen),張允崇(Yun-Chorng Chang) | |
| dc.subject.keyword | 乙型類澱粉蛋白,石墨烯,場效電晶體,生物感測器,支撐性磷脂雙層, | zh_TW |
| dc.subject.keyword | Alzheimer’s disease,amyloid-beta,graphene,field-effect transistor,biosensor,supported lipid bilayer, | en |
| dc.relation.page | 61 | |
| dc.identifier.doi | 10.6342/NTU201700721 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-03-28 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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