請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59585
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃念祖(Nien-Tsu Huang) | |
dc.contributor.author | Bo-Cheng Huang | en |
dc.contributor.author | 黃柏承 | zh_TW |
dc.date.accessioned | 2021-06-16T09:28:56Z | - |
dc.date.available | 2017-06-12 | |
dc.date.copyright | 2017-06-12 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-03-29 | |
dc.identifier.citation | [1] Harley Tse Raphael Wong. Lateral Flow Immunoassay. Humana Press,2009.
[2] Elisângela M. Linares et al. Enhancement of the detection limit for lateral flow immunoassays: evaluation and comparison of bioconjugates. Journal of immunological methods,375(1):264–270,2012. [3] Staffan Birnbaumetal. Latex-based thin-layer immunoaffinity chromatography for quantitation of protein analytes. Analytical biochemistry, 206(1):168–171,1992. [4] Maria Lönnberg and Jan Carlsson. Quantitative detection in the attomole range for immunochromatographic tests by means of a flatbed scanner. Analytical biochemistry,293(2):224–231,2001. [5] Kumi Inoue et al. A competitive immunochromatographic assay for testosterone based on electrochemical detection. Talanta,73(5):886–892,2007. [6] JK Horton, S Swinburne, and MJ O’Sullivan. A novel, rapid, single-step immunochromatographic procedure for the detection of mouse immunoglobulin. Journal of immunological methods, 140(1):131–134,1991. [7] Ryo Tanaka et al. A novel enhancement assay for immunochromatographic test strips using gold nanoparticles. Analytical and bioanalytical chemistry, 385(8): 1414–1420,2006. [8] Natalya V.Zaytseva et al. Multi-analyte single-membrane biosensor for the serotype-specific detection of dengue virus. Analytical and bioanalytical chemistry, 380(1): 46–53,2004. [9] George M Whitesides. The origins and the future of microfluidics. Nature, 442(7101):368–373,2006. [10] Xu Li, David R Ballerini, and Wei Shen. A perspective on paper-based microfluidics: current status and future trends. Biomicrofluidics,6(1):011301,2012. [11] Theo Vos et al. Years lived with disability (ylds) for 1160 sequelae of 289 diseases and injuries 1990–2010: a systematic analysis for the global burden of disease study 2010. The Lancet,380(9859):2163–2196,2013. [12] S.Listl et al. Global economic impact of dental diseases. Journal of dental research, page 0022034515602879,2015. [13] Poul E Petersen and Hiroshi Ogawa. The global burden of periodontal disease: to wards integration with chronic disease prevention and control. Periodontology 2000, 60(1):15–39,2012. [14] Blanca Urzúa et al. Yeast diversity in the oral microbiota of subjects with periodontitis: Candida albicans and candida dubliniensis colonize the periodontal pockets. Medical mycology,46(8):783–793,2008. [15] Nilminie Rathnayake et al. Salivary biomarkers of oral health–a cross-sectional study. Journal of Clinical Periodontology,40(2):140–147,2013. [16] Christoph A. Ramseier et al. Identification of pathogen and host-response markers correlated with periodontal disease. Journal of periodontology, 80(3):436–446,2009. [17] Geertruida A Posthuma-Trumpie, Jakob Korf, and Aart van Amerongen. Lateral flow (immuno) assay: its strengths, weaknesses, opportunities and threats. a literature survey. Analytical and bioanalytical chemistry,393(2):569–582,2009. [18] Muhammad Sajid, Abdel-Nasser Kawde, and Muhammad Daud. Designs, formats and applications of lateral flow assay: A literature review. Journal of Saudi Chemical Society,19(6):689–705,2015. [19] LauraAnfossietal. Lateral-flow immunoassays for mycotoxins and phycotoxins: a review. Analytical and bioanalytical chemistry,405(2-3):467–480,2013. [20] Babacar Ngom et al. Development and application of lateral flow test strip technology for detection of infectious agents and chemical contaminants: a review. Analytical and bioanalytical chemistry,397(3):1113–1135,2010. [21] Solomon A Berson and Rosalyn S Yalow. Quantitative aspects of the reaction between insulin and insulin-binding antibody. Journal of Clinical Investigation, 38(11):1996,1959. [22] Eva Engvall and Peter Perlmann. Enzyme-linked immunosorbent assay(elisa) quantitative assay of immunoglobulin g. Immunochemistry,8(9):871–874,1971. [23] Jacques M Singer and Charles M Plotz. The latex fixation test: I. application to the serologic diagnosis of rheumatoid arthritis. The American journal of medicine, 21(6):888–892,1956. [24] Shuo ang et al. Rapid determination of fumonisin b1 in food samples by enzyme linked immunosorbent assay and colloidal gold immunoassay. Journal of agricultural and food chemistry,54(7):2491–2495,2006. [25] A.Salomone et al. Reliability of detection of citrus tristeza virus by an immunochromatographic lateral flow assay in comparison with elisa. Journal of PlantPathology, pages43–48,2004. [26] Xiaolin Huang et al. Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: A review. Biosensors and Bioelectronics,75:166–180,2016. [27] Irina Yu Goryacheva, Pieterjan Lenain, and Sarah De Saeger. Nanosized labels for rapid immunotests. TrAC Trends in Analytical Chemistry,46:30–43,2013. [28] Aart van Amerongen et al. Quantitative computer image analysis of a human chorionic gonadotropin colloidal carbon dipstick assay. Clinica chimica acta, 229(1-2): 67–75,1994. [29] Juan Su et al. Quantitative detection of human chorionic gonadotropin antigen via immunogold chromatographic test strips. Analytical Methods,6(2):450–455,2014. [30] Can Zhang,Yan Zhang,and ShuoWang. Development of multianalyte flow-through and lateral-flow assays using gold particles and horseradish peroxidase as tracers for the rapid determination of carbaryl and endosulfan in agricultural products. Journal of agricultural and food chemistry,54(7):2502–2507,2006. [31] Arindam Pal and Tarun K Dhar. An analytical device for on-site immunoassay. demonstration of its applicability in semiquantitative detection of aflatoxin b1 in a batch of samples with ultrahigh sensitivity. Analytical chemistry, 76(1):98–104, 2004. [32] Namrata Sippy et al. Rapid electrochemical detection and identification of catalase positivemicro-organisms. BiosensorsandBioelectronics,18(5):741–749,2003. [33] Zarini Muhammad-Tahir and Evangelyn C Alocilja. A conductometric biosensor for biosecurity. Biosensors and Bioelectronics,18(5):813–819,2003. [34] Rong-Hwa Shyu et al. Colloidal gold-based immunochromatographic assay for detection of ricin. Toxicon,40(3):255–258,2002. [35] Pei Zhou et al. Nanocolloidal gold-based immunoassay for the detection of the n-methylcarbamate pesticide carbofuran. Journal of agricultural and food chemistry, 52(14):4355–4359,2004. [36] Wei Yang et al. A colloidal gold probe-based silver enhancement immunochromatographic assay for the rapid detection of abrin-a. Biosensors and Bioelectronics, 26(8):3710–3713,2011. [37] William E. Doering et al. Sers as a foundation for nanoscale, optically detected biologicallabels. AdvancedMaterials, 19(20):3100–3108,2007. [38] Carsten Sönnichsen et al. A molecular ruler based on plasmon coupling of single goldandsilvernanoparticles. Naturebiotechnology,23(6):741–745,2005. [39] Yuichi Oku et al. Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection. Journal of immunological methods,258(1):73–84,2001. [40] Anna Yu Kolosova et al. Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol. Analytical and bioanalytical chemistry,389(7-8):2103–2107,2007. [41] Kenneth F. Buechler et al. Simultaneous detection of seven drugs of abuse by the triage panel for drugs of abuse. Clinical chemistry,38(9):1678–1684,1992. [42] Steven D Schwartzbach and Tetsuaki Osafune. Immunoelectron microscopy: methodsandprotocols. Humana Press,2010. [43] Constance Oliver and eds. Maria Célia Jamur. Immunocytochemical methods and protocols. Humana Press,2010. [44] ed. Javois, Lorette C. Immunocytochemical methods and protocols, volume 115. Springer,1999. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59585 | - |
dc.description.abstract | 在 2010 年全球有 10.79% 的人口受牙周病的影響,也就是將近 7.5 億的人口總數,並造成約 539 億元的全球生產總值下降,造成巨大的經濟負擔。因此,為了解決牙周病衍生的各種社會問題,偵測早期牙周病並及時處理與治療,可大幅降低牙周病後期所需要的醫療開銷,降低國家在口腔疾病治療上的負擔。因此為了預防牙周病的發生,本論文開發了側向流免疫分析試紙,並以此裝置偵測牙周病的標準生物細胞因子-基質金屬蛋白酶 (Matrix metallopeptidase 9)。我們成功達成 (1) 金奈米粒子與 MMP-9 檢測抗體的鍵結;(2)MMP-9 抓捕抗體最佳化;(3) 樣本收集區過濾測試;(4)NC 薄膜 (Nitrocellulose) 進行 MMP-9 偵測和 (5) 銀離子強化等功能,以達成快速的檢測 MMP-9,並得到此裝置 MMP-9 的最低偵測極限 是 1.49 ng mL−1。在未來展望方面,我們希望此裝置能夠結合多重因子偵測並達到定點照護的概念。我們相信這個裝置將會對健康醫療,尤其在口腔照護方面,有長足的貢獻,並解決醫療資源短缺地區在牙周病偵測上的不便。 | zh_TW |
dc.description.abstract | In 2010, 10.79% of the world’s population, about 750 million people, is affected by chronic periodontitis. Productivity loss from periodontitis alone is approximately $53.99 billion each year, causing a huge economic burden around the world. Therefore,the early prediction and treatment of periodontitis is critical in relieving the economic burden and medical expenses in low-resource settings. In this thesis, we developed a lateral flow immunoassay (LFIA) test strip for periodontitis diagnosis, and used the LFIA test strip to detect MMP-9, which is a standard biomarker for periodontitis. We achieved: (1) conjugation of MMP-9 antibody to AuNPs;(2) capture antibody optimization;(3)sample pad filtration;(4)MMP-9 detection by the 1/2 LFIA test strips and(5) sliver enhancement, and derive the limit of detection (LOD) of 1.49 ngmL−1. In the future, we hope this device can be applied in multiplex detection and achieve the concept of point-of-care (POC).We believe this development will be greatly contributed to health care and welfare,especially in dental care,and resource-limited country. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:28:56Z (GMT). No. of bitstreams: 1 ntu-106-R03945014-1.pdf: 9148000 bytes, checksum: 545a5748127f595cf6c3a1950469c69f (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試會定書 i
致謝 ii 中文要 iv Abstract v Contents vi ListofFigures viii ListofTables xi 1 Introduction 1 1.1 Background . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.2 Motivation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2 Literaturereview 4 2.1 OverviewofLFIA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.2 TheHistoryofLFIA . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2.3 ComponentsofLFIA . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 2.3.1 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2.3.2 Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2.3.3 MultiplexLFIA . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 3 MethodandMaterials 17 3.1 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 3.2 PreparationoftheDevice . . . . . . . . . . . . . . . . . . . . . . . . . . 18 3.3 AuNPs-MMP-9AbConjugates . . . . . . . . . . . . . . . . . . . . . . . 19 3.4 TheProcedureofLFIADetection . . . . . . . . . . . . . . . . . . . . . 21 3.5 SignalDetectionandProcess . . . . . . . . . . . . . . . . . . . . . . . . 22 4 ResultsandDiscussion 24 4.1 AuNPsBufferOptimization . . . . . . . . . . . . . . . . . . . . . . . . 24 4.2 DetectionAntibodyOptimization. . . . . . . . . . . . . . . . . . . . . . 25 4.2.1 MabI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 4.2.2 MabII. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 4.3 CaptureAntibodyOptimization . . . . . . . . . . . . . . . . . . . . . . 30 4.4 MMP-9Detectionbyusingthe1/2TestStrip . . . . . . . . . . . . . . . 34 4.4.1 SilverEnhancement . . . . . . . . . . . . . . . . . . . . . . . . 36 4.5 MucinsFiltration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 5 Conclusion 41 6 Futurework 43 Bibliography 46 | |
dc.language.iso | en | |
dc.title | 開發側向流免疫分析試紙進行基質金屬蛋白酶偵測 | zh_TW |
dc.title | Developing a Lateral Flow Immunoassay Test Strip for Matrix Metallopeptidase 9 Detection | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳奕帆(Yih-Fan Chen),林致廷(Chih-Ting Lin),黃榮山(Long-Sun Huang) | |
dc.subject.keyword | 試紙,微流道,金屬蛋白?, | zh_TW |
dc.subject.keyword | test strip,microfluidic,MMP-9, | en |
dc.relation.page | 51 | |
dc.identifier.doi | 10.6342/NTU201700725 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-03-30 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
顯示於系所單位: | 生醫電子與資訊學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-1.pdf 目前未授權公開取用 | 8.93 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。