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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 魏安祺(An-Chi Wei) | |
dc.contributor.author | Chien-Hsun Huang | en |
dc.contributor.author | 黃建勛 | zh_TW |
dc.date.accessioned | 2021-06-16T09:26:19Z | - |
dc.date.available | 2026-02-23 | |
dc.date.copyright | 2021-03-30 | |
dc.date.issued | 2021 | |
dc.date.submitted | 2021-02-24 | |
dc.identifier.citation | 1. Wang, X., et al., Interplay between up-regulation of cytochrome-c-oxidase and hemoglobin oxygenation induced by near-infrared laser. Sci Rep, 2016. 6: p. 30540. 2. Ferraresi, C., et al., Low-level laser (light) therapy increases mitochondrial membrane potential and ATP synthesis in C2C12 myotubes with a peak response at 3-6 h. Photochem Photobiol, 2015. 91(2): p. 411-6. 3. Mester, E., B. Szende, and P. Gärtner, [The effect of laser beams on the growth of hair in mice]. Radiobiol Radiother (Berl), 1968. 9(5): p. 621-6. 4. Wong-Riley, M.T., et al., Photobiomodulation directly benefits primary neurons functionally inactivated by toxins: role of cytochrome c oxidase. J Biol Chem, 2005. 280(6): p. 4761-71. 5. Karu, T.I. and N.I. Afanas'eva, [Cytochrome c oxidase as the primary photoacceptor upon laser exposure of cultured cells to visible and near IR-range light]. Dokl Akad Nauk, 1995. 342(5): p. 693-5. 6. Caterina, M.J. and Z. Pang, TRP Channels in Skin Biology and Pathophysiology. Pharmaceuticals (Basel), 2016. 9(4). 7. Salehpour, F., et al., Brain Photobiomodulation Therapy: a Narrative Review. Mol Neurobiol, 2018. 55(8): p. 6601-6636. 8. Sarti, P., et al., Cytochrome c oxidase and nitric oxide in action: molecular mechanisms and pathophysiological implications. Biochim Biophys Acta, 2012. 1817(4): p. 610-9. 9. Hamblin, M.R., Mechanisms and Mitochondrial Redox Signaling in Photobiomodulation. Photochem Photobiol, 2018. 94(2): p. 199-212. 10. Pannala, V.R., A.K. Camara, and R.K. Dash, Modeling the detailed kinetics of mitochondrial cytochrome c oxidase: Catalytic mechanism and nitric oxide inhibition. J Appl Physiol (1985), 2016. 121(5): p. 1196-1207. 11. de Freitas, L.F. and M.R. Hamblin, Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy. IEEE J Sel Top Quantum Electron, 2016. 22(3). 12. Huang, Y.Y., et al., Biphasic dose response in low level light therapy - an update. Dose Response, 2011. 9(4): p. 602-18. 13. McGuff, P.E., R.A. Deterling, Jr., and L.S. Gottlieb, Tumoricidal effect of laser energy on experimental and human malignant tumors. N Engl J Med, 1965. 273(9): p. 490-2. 14. Gál, P., et al., Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and corticosteroid-treated rats. Lasers Med Sci, 2009. 24(4): p. 539-47. 15. Hamblin, M. and T. Demidova, Mechanisms of low level light therapy. Proc SPIE, 2006. 6140: p. 1-12. 16. Mason, M., P. Nicholls, and C. Cooper, Re-evaluation of the Near Infrared spectra of mitochondrial cytochrome c oxidase: implications for non invasive in vivo monitoring of tissues. Biochimica et biophysica acta, 2014. 1837. 17. Avci, P., et al., Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg, 2013. 32(1): p. 41-52. 18. Quaresima, V., S. Bisconti, and M. Ferrari, A brief review on the use of functional near-infrared spectroscopy (fNIRS) for language imaging studies in human newborns and adults. Brain Lang, 2012. 121(2): p. 79-89. 19. Kolyva, C., et al., Cytochrome c oxidase response to changes in cerebral oxygen delivery in the adult brain shows higher brain-specificity than haemoglobin. Neuroimage, 2014. 85 Pt 1(Pt 1): p. 234-44. 20. Tachtsidis, I., et al., Functional optical topography analysis using statistical parametric mapping (SPM) methodology with and without physiological confounds. Advances in experimental medicine and biology, 2010. 662: p. 237-243. 21. Kolyva, C., et al., Systematic investigation of changes in oxidized cerebral cytochrome c oxidase concentration during frontal lobe activation in healthy adults. Biomed Opt Express, 2012. 3(10): p. 2550-66. 22. Sanderson, T.H., et al., Inhibitory modulation of cytochrome c oxidase activity with specific near-infrared light wavelengths attenuates brain ischemia/reperfusion injury. Scientific Reports, 2018. 8(1): p. 3481. 23. Butt, W.D. and D. Keilin, Absorption Spectra and Some Other Properties of Cytochrome c and of Its Compounds with Ligands. Proceedings of the Royal Society of London. Series B, Biological Sciences, 1962. 156(965): p. 429-458. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59516 | - |
dc.description.abstract | 光生物調節(Photobiomodulation)是一種新穎且具有潛力的治療方式,一方面光治療本身是無創的,且光治療使用的波長較長,對於人體的傷害較低;另一方面是LED的取得相當容易,不論是臨床治療或是居家應用,都非常容易實行。光生物調節的應用相當廣泛,根據治療的部位不同會達到不同的治療效果,例如:促進傷口復原、舒緩肌肉痠痛以及提升大腦的認知能力等,不只是使用在特定病患上,而是幾乎適用於每一個人。Cytochrome c Oxidase,電子傳遞鏈裡其中一個蛋白質複合體,是促進氧氣還原成水的關鍵酵素。在近期的研究中被認為是參與光生物調節中,吸收紅外光和近紅外光的重要的發色團(Chromophore)。 在本研究中,我們使用電極作為偵測氧氣消耗的工具,測量人類心肌細胞AC16在照射750 nm、810 nm、940 nm及1050 nm(約2 J/cm2,持續照射五分鐘)後得到的不同耗氧結果;從結果中得到750 nm及940 nm會造成粒線體耗氧率下降,810 nm及1050 nm則會造成粒線體耗氧率上升。同時本篇研究除了AC16之外,還對其他細胞株Panc-1、HEK-293、HepG2和IMR-32照射1050 nm(約2 J/cm2,持續照射五分鐘)光處理,並測量各株細胞對其控制組氧氣消耗的結果,從實驗結果發現,AC16及HepG2在照射1050 nm對上其對照組的氧氣消耗有顯著提升。本篇研究還使用了Cytochrome c Oxidase Assay做為粒線體活性測量的工具,對細胞照射810 nm及1050 nm(1.07~3.06 J/cm2,持續照射五分鐘),發現照射810 nm後,細胞活性有顯著提升。最後對粒線體染TMRM,使用Confocal拍攝螢光訊號來確認膜電位的高低,我們對細胞AC16、Panc-1、HEK-293、HepG2和IMR-32細胞照射1050 nm(6.37 mW/cm2),發現照射後能夠減緩AC16膜電位下降。儘管對於光生物調節的機制尚未完全了解,但從多篇研究及本篇實驗結果可以確定,使用近紅外光是一種很有潛力的治療手段。 | zh_TW |
dc.description.abstract | Photobiomodulation has been applied as noninvasive intervention to regulate cellular functions in animal models, and clinical applications. Cytochrome c oxidase (CCO) is a protein complex of the electron transport chain that has potential chromophores for infrared light absorption, which can modulate mitochondrial respiration and energy production. The photobiomodulation mechanisms of CCO may come from direct changes in enzymatic activity or indirect responses involving the activation of transcription factors or secondary signaling pathways. In this thesis, we focused on acute infrared treatment effects on mitochondria respiration in human cell lines under different wavelengths and intensities. The oxygen consumption rate of suspended cultured cells was measured using a Clarke-type electrode under different light wavelengths and intensities that ranged from near-infrared to short-wavelength infrared. In the wavelength ranges, 810 nm and 1050 nm showed upregulation of mitochondrial respiration, while 750 nm and 940 nm showed downregulation. We observed the same trend in a parallel CCO activity colorimetric assay measuring the absorption of cytochrome c at 550 nm. Among five tested cell lines with different human organ origins ( AC16, PANC-1, HEK293, HepG2, and IMR32 cell lines), the HepG2 cell line showed the greatest activity increase under low energy light treatment of 1050 nm (1.07~3.06 J/cm2). The AC16 cells maintained the TMRM fluorescent intensity best among these five cell lines. Although the exact mechanisms of how infrared light modulates mitochondria respiration are not fully understood, short-wavelength infrared (> 1000 nm) is an attractive candidate for therapeutic intervention because it provides noninvasive drug-free photomodulation of mitochondrial energetics while indirectly controlling ΔΨm and ROS production with the benefit of better human tissue penetration than near-infrared light (< 1000 nm). | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:26:19Z (GMT). No. of bitstreams: 1 U0001-0702202123260000.pdf: 3033896 bytes, checksum: e453da14b0a5cb5186b6197d0f0430de (MD5) Previous issue date: 2021 | en |
dc.description.tableofcontents | 中文摘要 3 Abstract 4 目錄 5 圖目錄 7 表目錄 9 第一章:緒論 10 第一節:研究背景 10 光生物調節 10 發色團 10 Cytochrome c Oxidase 11 假說 12 紅光與近紅外光 13 雙向劑量效應 13 第二節:文獻探討 15 光學窗口(Optical window) 15 照射人體前臂 16 照射細胞株 19 抑制性波長 20 第三節:研究動機 21 第二章:研究方法與材料 22 第一節:細胞培養 22 第二節:儀器 23 第三節:光學平台搭建 25 第四節:尋找實驗條件 26 第五節:氧氣消耗實驗 27 照射劑量的決定 27 810 nm光源照射於不同細胞 28 對AC16細胞照射不同波長之光源 29 1050 nm光源照射於不同細胞 30 第六節:Cytochrome c oxidase assay 32 第七節:細胞影像 33 第八節:資料統計與分析 35 第三章:結果 36 第一節:氧氣消耗實驗 36 確認光照條件 36 810 nm光源照射於不同細胞 37 對AC16照射不同波長之光源 38 1050 nm光源照射於不同細胞 39 第二節:Cytochrome c Oxidase Assay 40 第三節:細胞影像 42 第四章:討論 44 第五章:結論 47 參考文獻 48 | |
dc.language.iso | zh-TW | |
dc.title | 使用寬範圍近紅外光對細胞色素c氧化酶活性的光生物調節 | zh_TW |
dc.title | Broad range near infrared photobiomodulation of cytochrome c oxidase activity | en |
dc.type | Thesis | |
dc.date.schoolyear | 109-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃立達(Li-Da Huang),宋孔彬(Kung-Bin Sung),何亦平(Yi-Ping Ho) | |
dc.subject.keyword | 光生物調節,粒線體,細胞色素c氧化酶,近紅外光, | zh_TW |
dc.subject.keyword | photobiomodulation,mitochondria,cytochrome c oxidase,near infrared, | en |
dc.relation.page | 50 | |
dc.identifier.doi | 10.6342/NTU202100653 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2021-02-25 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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