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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 蔡坤憲(Kun-Hsien Tsai) | |
dc.contributor.author | Chien-Ling Su | en |
dc.contributor.author | 蘇千玲 | zh_TW |
dc.date.accessioned | 2021-06-16T09:25:42Z | - |
dc.date.available | 2019-09-12 | |
dc.date.copyright | 2017-09-12 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-06-08 | |
dc.identifier.citation | 1.WHO. World Health Day 2014: Preventing vector-borne diseases. Available at: http://www.who.int/mediacentre/news/releases/2014/small-bite-big-threat/en/
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Philos Trans R Soc Lond B Biol Sci. 2015; 370(1665): 20130561. 50.盧孟明、卓盈旻、李思瑩等:臺灣氣候變化:1911~2009年資料分析。大氣科學2012; 40(3): 297-321。 51.蘇慧貞、吳佩芝、龍世俊等:氣候暖化對台灣防疫之風險與因應對策相關研究。行政院衛生署疾病管制局98年度科技研究發展計畫研究報告2009。 52.Luis FK, Shaohong L, Gabriel M. et al. Co-distribution and co-infection of chikungunya and dengue viruses. BMC Infect Dis. 2016; 16: 84. 53.Chang SF, Su CL, Shu PY, et al. Concurrent isolation of Chikungunya virus and Dengue virus from a patient with coinfection resulting from a trip to Singapore. J Clin Microbiol. 2010; 48 (12): 4586-89. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59498 | - |
dc.description.abstract | 日本腦炎、登革熱和茲卡病毒感染症是在臺灣疾病管制署列為通報的蚊媒性傳染病。其中,日本腦炎病毒感染發生在大多數亞洲國家也包括臺灣在內,此病毒是亞洲地區最常見的腦炎主因。根據過去文獻紀錄,近幾年日本腦炎病毒的基因型別已由原來的第三型轉變成基因型第一型。登革熱是登革病毒所引起,為臺灣最常見的蚊媒性疾病,在臺灣主要是因為通過密切的商業聯繫與不斷輸入鄰近東南亞國家的登革病毒,致使臺灣每年都會有大大小小不同的的疫情發生,由於每年的流行株序列不同,可以證實登革熱並非地方性流行病。自從2005年以來,茲卡病毒感染症疫情已發生在65個國家,主要是亞洲和美洲地區,多數被茲卡病毒感染的人是沒有症狀的,但有少部分的人症狀卻非常嚴重,在臺灣2016年共有13例境外移入茲卡感染症個案。本研究於2016年期間,於臺灣北中南東區,以乾冰掛網結合捕蚊器和人工掃網進行蚊蟲採集,採集之成蚊先進行型態分類,並進行蚊媒病毒的檢測和特性分析。在本研究中,利用螢光定量反轉錄聚合酶連鎖反應方法,使用廣用性的引子組來檢測病媒蚊感染病毒情況。使用之引子組涵蓋黃病毒科、套膜病毒科、呼腸病毒科和布尼亞病毒科。篩檢結果顯示:在臺灣的養豬場、公園和水鳥棲息地被採集到的成蚊共有19種,總計22,042隻蚊蟲,分成572池,其中三斑家蚊是豬場和濕地中最常見的蚊種。黃病毒螢光定量反轉錄聚合酶連鎖反應檢測顯示共有99池為陽性,這些陽性池的蚊種計有三斑家蚊 (81池)、環蚊家蚊 (3池)、白頭家蚊 (1池)、中華瘧蚊 (6池)、熱帶家蚊 (1池)、白腹叢蚊 (1池)和地下家蚊 (6池)。病毒檢驗陽性檢體經成功培養和定序分析後,培養確認有日本腦炎病毒 (67池)與家蚊黃病毒 (5池)。日本腦炎病毒E基因定序及演化親緣性分析顯示:2016年分離到的所有日本腦炎病毒株均屬於基因型第一型,臺灣日本腦炎病毒分離株與中國大陸、越南、日本的日本腦炎病毒株的親緣關係最為接近。由於氣候變遷加劇,臺灣地區未來也可能發生登革熱、日本腦炎、茲卡病毒及其他蚊媒病毒傳染病的共同流行,政府應更加強病媒性傳染病監測,成立病媒性傳染病研究及防治中心,並配合實驗室為基礎的檢驗系統,有系統的進行各種病媒性病毒的監測、檢驗與流行病學研究,以降低病媒傳染病的發生。 | zh_TW |
dc.description.abstract | Japanese encephalitis, dengue fever and Zika virus (ZIKV) infection are reported as notified of mosquito-borne infectious diseases in Taiwan's CDC. Japanese encephalitis virus (JEV) infection occurs throughout most of Asian countries including Taiwan and is the most common cause of encephalitis in that region. The literature indicated that JEV strains had shifted from GIII to GI over the past years. Dengue fever is caused by dengue virus, the most common mosquito-borne disease in Taiwan. Mainly because through close business ties with neighboring countries in Southeast Asia continue to enter the dengue virus, resulting in Taiwan every year there are different sizes of outbreak, due to the annual sequence of different strains, it was confirmed that dengue is not endemic. ZIKV outbreaks have occurred in 65 countries, mainly in Asia and the America, since 2015. Most people with ZIKV infection have no symptoms or mild disease. In Taiwan, there were 13 imported ZIKA cases in 2016. During the period of 2016, mosquitoes were collected and collected by integrate binding dry ice hanging mosquito nets and sweeping net in Taiwan. The mosquitoes were collected and the mosquito-borne virus was monitored and analyzed. In this study, we developed multiple real-time fluorescence quantitative RT-PCR using virus Genus (or group) consensus primer sets to detect flavivirdae, togaviridae, reoviridae and bunyavirdae. The results showed that there were 19 species of mosquitoes collected in a wide range of pig farms, parks and waterbirds habitats in Taiwan, with a total number of 22,042 mosquitoes divided into 572 pools. Cx. tritaeniorhynchus was the most common mosquito species found in the pig farms and wetlands. A total number of 99 tested positive for flavivirus by RT-PCR, including Cx. tritaeniorhynchus (n=81), Cx. annulus (n=3), Cx. fuscocephala (n=1), Anopheles sinensis (n=6), Cx. quinquefasciatus (n=1), Armigeres subalbatus (n=1), and Cx. pipiens molestus (n=6). All the mosquito pools were extracted for virus isolation and were proved as JEV (67 pools) and Culex flavivirus (5 pools). Phylogenetic analysis indicated that all the JEV strains isolated in 2016 belong to genotype I. The phylogenetic analysis of JEV isolates from Taiwan showed a high correlation with JEV isolates from China, Vietnam and Japan. As the climate change intensifies, the future of Taiwan may suffer from dengue fever, Japanese encephalitis, ZIKV and other mosquito-borne infectious diseases, the Government should put more effort on surveillance of infectious diseases, and invest more resource to establish mosquito-borne infectious disease research and prevention center. It is expected to control the mosquito-borne diseases with the infrastructure of laboratory-based inspection system surveillance, diagnosis, as well as epidemiological analysis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:25:42Z (GMT). No. of bitstreams: 1 ntu-106-R02847045-1.pdf: 1653995 bytes, checksum: 761f6518292094a5d0b8811e9bb0abfa (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試委員審定書 i
謝辭 ii 中文摘要 iv 英文摘要 vi 第一章 導論 1 第一節 實習單位簡介 1 第二節 研究動機與目的 2 第三節 文獻探討 4 第二章 研究方法 7 第一節 材料試劑及儀器 7 第二節 病蚊媒採集 10 第三節 形態鑑定與分類 12 第四節 病媒蚊研磨 12 第五節 核酸萃取 13 第六節 引子的設計與合成 14 第七節 多重螢光定量反轉錄聚合酶連鎖反應 15 第八節 病毒分離鑑定及核酸定序 16 第九節 統計分析 17 第三章 結果 18 第一節 病蚊媒採集與統計 18 第二節 螢光定量反轉錄聚合酶連鎖反應結果 19 第三節 不同地區螢光定量反轉錄聚合酶連鎖反應陽性率 19 第四節 陽性池病毒分離與鑑定 20 第五節 病媒蚊日本腦炎病毒結構基因序列分析 20 第六節 統計分析 20 第四章 討論 22 參考文獻 32 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣地區蚊媒病毒之監測與特性分析 | zh_TW |
dc.title | Surveillance and Characterization of Mosquito-borne Viruses in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 舒佩芸(Pei-Yun Shu),陳維鈞(Wei-June Chen) | |
dc.subject.keyword | 監測,蚊媒病毒,日本腦炎病毒,定量反轉錄聚合?連鎖反應,三斑家蚊, | zh_TW |
dc.subject.keyword | surveillance,mosquito-borne viruses,Japanese encephalitis virus,quantitative RT-PCR,Cx. tritaeniorhynchus, | en |
dc.relation.page | 50 | |
dc.identifier.doi | 10.6342/NTU201700915 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-06-09 | |
dc.contributor.author-college | 公共衛生學院 | zh_TW |
dc.contributor.author-dept | 公共衛生碩士學位學程 | zh_TW |
顯示於系所單位: | 公共衛生碩士學位學程 |
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