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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 潘俊良(Chun-Liang Pan) | |
| dc.contributor.author | Sin-Ying Jiang | en |
| dc.contributor.author | 蔣欣穎 | zh_TW |
| dc.date.accessioned | 2021-06-16T09:24:14Z | - |
| dc.date.available | 2017-09-12 | |
| dc.date.copyright | 2017-09-12 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-06-20 | |
| dc.identifier.citation | Ando, S. (2012). Neuronal dysfunction with aging and its amelioration. Proc Jpn Acad Ser B Phys Biol Sci, 88(6), 266-282.
Bargmann, C. I., Hartwieg, E., & Horvitz, H. R. (1993). Odorant-Selective Genes and Neurons Mediate Olfaction in C-Elegans. Cell, 74(3), 515-527. Bishop, N. A., Lu, T., & Yankner, B. A. (2010). Neural mechanisms of ageing and cognitive decline. Nature, 464(7288), 529-535. Bloss, E. B., Janssen, W. G., Ohm, D. T., Yuk, F. J., Wadsworth, S., Saardi, K. M., . . . Morrison, J. H. (2011). Evidence for reduced experience-dependent dendritic spine plasticity in the aging prefrontal cortex. J Neurosci, 31(21), 7831-7839. Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics, 77(1), 71-94. Coburn, C. M., & Bargmann, C. I. (1996). A putative cyclic nucleotide-gated channel is required for sensory development and function in C-elegans. Neuron, 17(4), 695-706. Dumitriu, D., Hao, J., Hara, Y., Kaufmann, J., Janssen, W. G., Lou, W., . . . Morrison, J. H. (2010). Selective changes in thin spine density and morphology in monkey prefrontal cortex correlate with aging-related cognitive impairment. J Neurosci, 30(22), 7507-7515. Falk, N., Losl, M., Schroder, N., & Giessl, A. (2015). Specialized Cilia in Mammalian Sensory Systems. Cells, 4(3), 500-519. Farajnia, S., Deboer, T., Rohling, J. H., Meijer, J. H., & Michel, S. (2014). Aging of the suprachiasmatic clock. Neuroscientist, 20(1), 44-55. Fontaine, S. N., Ingram, A., Cloyd, R. A., Meier, S. E., Miller, E., Lyons, D., . . . Abisambra, J. F. (2017). Identification of changes in neuronal function as a consequence of aging and tauopathic neurodegeneration using a novel and sensitive magnetic resonance imaging approach. Neurobiol Aging, 56, 78-86. Garigan, D., Hsu, A. L., Fraser, A. G., Kamath, R. S., Ahringer, J., & Kenyon, C. (2002). Genetic analysis of tissue aging in Caenorhabditis elegans: a role for heat-shock factor and bacterial proliferation. Genetics, 161(3), 1101-1112. Idowu, A. J., Olatunji, B., II, & Olagunju, J. A. (2017). Neuronal Function in Male Sprague Dawley Rats During Normal Ageing. Niger J Physiol Sci, 31(2), 127-131. Irvine, K. A., & Blakemore, W. F. (2006). Age increases axon loss associated with primary demyelination in cuprizone-induced demyelination in C57BL/6 mice. J Neuroimmunol, 175(1-2), 69-76. Jiang, H. C., Hsu, J. M., Yen, C. P., Chao, C. C., Chen, R. H., & Pan, C. L. (2015). Neural activity and CaMKII protect mitochondria from fragmentation in aging Caenorhabditis elegans neurons. Proc Natl Acad Sci U S A, 112(28), 8768-8773. Kabaso, D., Coskren, P. J., Henry, B. I., Hof, P. R., & Wearne, S. L. (2009). The Electrotonic Structure of Pyramidal Neurons Contributing to Prefrontal Cortical Circuits in Macaque Monkeys Is Significantly Altered in Aging. Cerebral Cortex, 19(10), 2248-2268. Kaplan, O. I., Doroquez, D. B., Cevik, S., Bowie, R. V., Clarke, L., Sanders, A. A., . . . Blacque, O. E. (2012). Endocytosis genes facilitate protein and membrane transport in C. elegans sensory cilia. Curr Biol, 22(6), 451-460. Keuker, J. I., de Biurrun, G., Luiten, P. G., & Fuchs, E. (2004). Preservation of hippocampal neuron numbers and hippocampal subfield volumes in behaviorally characterized aged tree shrews. J Comp Neurol, 468(4), 509-517. Li, Y. F., Healy, E. W., Drane, J. W., & Zhang, J. (2006). Comorbidity between and risk factors for severe hearing and memory impairment in older Americans. Preventive Medicine, 43(5), 416-421. Marshall, J., Grindle, J., Ansell, P. L., & Borwein, B. (1979). Convolution in human rods: an ageing process. Br J Ophthalmol, 63(3), 181-187. Mello, C. C., Kramer, J. M., Stinchcomb, D., & Ambros, V. (1991). Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J, 10(12), 3959-3970. Mobley, A. S., Rodriguez-Gil, D. J., Imamura, F., & Greer, C. A. (2014). Aging in the olfactory system. Trends Neurosci, 37(2), 77-84. Morrison, J. H., & Baxter, M. G. (2012). The ageing cortical synapse: hallmarks and implications for cognitive decline. Nature Reviews Neuroscience, 13(4), 240-250. Mukhopadhyay, S., Lu, Y., Shaham, S., & Sengupta, P. (2008). Sensory signaling-dependent remodeling of olfactory cilia architecture in C. elegans. Dev Cell, 14(5), 762-774. Murman, D. L. (2015). The Impact of Age on Cognition. Semin Hear, 36(3), 111-121. Nag, T. C., & Wadhwa, S. (2012). Ultrastructure of the human retina in aging and various pathological states. Micron, 43(7), 759-781. Ogg, S., Paradis, S., Gottlieb, S., Patterson, G. I., Lee, L., Tissenbaum, H. A., & Ruvkun, G. (1997). The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans. Nature, 389(6654), 994-999. Pan, C. L., Peng, C. Y., Chen, C. H., & McIntire, S. (2011). Genetic analysis of age-dependent defects of the Caenorhabditis elegans touch receptor neurons. Proc Natl Acad Sci U S A, 108(22), 9274-9279. Pannese, E. (2011). Morphological changes in nerve cells during normal aging. Brain Struct Funct, 216(2), 85-89. Rosli, Y., Breckenridge, L. J., & Smith, R. A. (1999). An ultrastructural study of age-related changes in mouse olfactory epithelium. J Electron Microsc (Tokyo), 48(1), 77-84. Tank, E. M., Rodgers, K. E., & Kenyon, C. (2011). Spontaneous age-related neurite branching in Caenorhabditis elegans. J Neurosci, 31(25), 9279-9288. Toth, M. L., Melentijevic, I., Shah, L., Bhatia, A., Lu, K., Talwar, A., . . . Driscoll, M. (2012). Neurite sprouting and synapse deterioration in the aging Caenorhabditis elegans nervous system. J Neurosci, 32(26), 8778-8790. Troemel, E. R., Chou, J. H., Dwyer, N. D., Colbert, H. A., & Bargmann, C. I. (1995). Divergent seven transmembrane receptors are candidate chemosensory receptors in C. elegans. Cell, 83(2), 207-218. Troemel, E. R., Kimmel, B. E., & Bargmann, C. I. (1997). Reprogramming chemotaxis responses: Sensory neurons define olfactory preferences in C-elegans. Cell, 91(2), 161-169. Troemel, E. R., Sagasti, A., & Bargmann, C. I. (1999). Lateral signaling mediated by axon contact and calcium entry regulates asymmetric odorant receptor expression in C-elegans. Cell, 99(4), 387-398. Vitale, S., Cotch, M. F., & Sperduto, R. D. (2006). Prevalence of visual impairment in the United States. JAMA, 295(18), 2158-2163. Wang, J., & Deretic, D. (2014). Molecular complexes that direct rhodopsin transport to primary cilia. Progress in Retinal and Eye Research, 38, 1-19. Wes, P. D., & Bargmann, C. I. (2001). C-elegans odour discrimination requires asymmetric diversity in olfactory neurons. Nature, 410(6829), 698-701. Wickremaratchi, M. M., & Llewelyn, J. G. (2006). Effects of ageing on touch. Postgrad Med J, 82(967), 301-304. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59453 | - |
| dc.description.abstract | 感覺纖毛是一種由微管構成的胞器，主要功能為偵測化學性或物理性的刺激。老化會造成感覺系統的功能喪失。我們先前發現，在線蟲中的一個感覺器官，amphid，其中具有纖毛的感覺神經在老化時會有結構上的變化，像是感溫神經AFD在老化時會產生微絨毛的減少以及纖毛的膨大，或是化學感覺神經ASI和ASE在老化時所產生的纖毛旁膜室的擴大。為了描述其他在amphid中的感覺神經在老化時的變化，我們以嗅覺神經AWB和AWC作為研究對象。我們觀察到老化時，AWB的纖毛會有局部膨大的現象，而AWC的纖毛則會有捲曲以及出現堆積物的現象。當我們在AWC嗅覺神經中表現TAX-2陽離子通道而使神經活化，發現可以減緩老化所造成的感覺纖毛的形態變化，這代表神經活性在老化時扮演著維持纖毛形狀的角色。總括來說，我們發現嗅覺神經纖毛在老化時有形狀上的改變，這種老化相關的纖毛形態變化，可作為神經老化的指標。 | zh_TW |
| dc.description.abstract | Sensory cilia, microtubule-based subcellular organelles, are involved in sensing chemical or mechanical stimuli. Dysfunction of the sensory system occurs during aging. Previously, we have described age-related morphological changes in amphid sensory cilia in C. elegans, such as reduced AFD ciliated endings and enlargement of periciliary membrane compartments of ASI and ASE neurons. To characterize the morphological changes of other amphid sensory neurons in C. elegans during aging, we studied the olfactory sensory neurons, AWB and AWC. We found focal enlargement of AWB cilia and curling of AWC cilia. Overexpression of TAX-2 cation channel, which activates the neurons, improves age-related AWC cilia defects, suggesting a role for neural activity in sensory cilia maintenance during aging. In summary, we found that olfactory sensory cilia showed morphological deterioration during aging, which may be one of the indicators of aging of the sensory neurons. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T09:24:14Z (GMT). No. of bitstreams: 1 ntu-106-R04448011-1.pdf: 1390680 bytes, checksum: 058e78fe73d4414c2f7bd9676e6434b4 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 口試委員會審定書………………………………..……………...………..#
ACKNOWLEDGEMENT………..……………………………..……..….. i 中文摘要…………………………………………………..……...……… iii ABSTRACT………………………….…………………………...……… iv CONTENTS……………………………….……………………...………. v Chapter 1 INTRODUCTION………………………...……………….. 1 1.1 Age-Related Changes in the Nervous System……...……….….. 1 1.2 Aging in the Sensory Nervous System…….…………...………. 2 1.3 Anatomy and Function of Amphid Sensory Neurons in C. elegans…………………………………………………...…………… 4 1.4 The Ciliary Morphology and Functions of AWB and AWC Neurons…………………………………………...………………..…. 5 Chapter 2 MATERIALS and METHODS………..…………………... 8 2.1 C. elegans Strains and Genetics………..……………………….. 8 2.2 Molecular Biology……………………………….…...…...……. 8 2.3 Fluorescence Microscopy………………....…………....………. 8 2.4 Analysis of Aging Phenotypes.…………….…………......….…. 9 Chapter 3 RESULTS…………………………...…………………….. 10 3.1 The Cilia of AWB and AWC Olfactory Neurons Show Age-Dependent Morphological Changes………………...……………….. 10 3.2 Lifespan Manipulation can Control the Evolution of AWC Cilia Defects…………….…………………………………….......………. 11 3.3 Endocytic Marker can Label PCMC of AWC Neurons……..…. 12 3.4 Localization of Cyclic Nucleotide-Gated Ion Channel in AWC Neurons.………………………………………………………......…. 12 Chapter 4 DISCUSSION……………………………………….…….. 14 4.1 AWB and AWC Olfactory Sensory Neurons Showed Age-Related Cilia Defects……………………………………...…………...…….. 14 4.2 Neural Activity Role on Cilia Maintenance during Aging…...... 15 4.3 Morphological Changes in AWB Neurons during Aging……… 15 4.4 Morphological Changes in AWC Neurons during Aging…….... 16 Chapter 5 FIGURES…………….…………..………………….…….. 17 Chapter 6 REFERENCE………………….……..…………...……..... 38 | |
| dc.language.iso | zh-TW | |
| dc.subject | 線蟲 | zh_TW |
| dc.subject | 神經老化 | zh_TW |
| dc.subject | 纖毛 | zh_TW |
| dc.subject | cilia | en |
| dc.subject | neuronal aging | en |
| dc.subject | C. elegans | en |
| dc.title | 老化對線蟲嗅覺神經的影響 | zh_TW |
| dc.title | Characterization of age-related changes in olfactory sensory neurons in C. elegans | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡欣祐(Hsin-Yue Tsai),吳瑞菁(Jui-Ching Wu) | |
| dc.subject.keyword | 線蟲,神經老化,纖毛, | zh_TW |
| dc.subject.keyword | C. elegans,neuronal aging,cilia, | en |
| dc.relation.page | 42 | |
| dc.identifier.doi | 10.6342/NTU201701002 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-06-20 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
| 顯示於系所單位： | 分子醫學研究所 | |
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