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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 潘子明 | |
| dc.contributor.author | Sheng-Fang Huang | en |
| dc.contributor.author | 黃聖方 | zh_TW |
| dc.date.accessioned | 2021-06-16T09:22:08Z | - |
| dc.date.available | 2019-07-17 | |
| dc.date.copyright | 2017-07-17 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-06-26 | |
| dc.identifier.citation | 行政院衛生福利部。2003。健康食品之胃腸功能改善評估方法。衛署食字第 0920401629 號公告修正。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59384 | - |
| dc.description.abstract | 腸道菌相在體內扮演了許多角色，具有調節免疫、血脂、血糖等功能，也可以提供腸道細胞所需營養及能量，並可以協助宿主抵抗病原菌入侵。一旦菌相發生紊亂可能會導致疾病發生，而益生菌及發酵乳先前已有許多文獻證實其調節腸道菌相之功效。本研究使用含 Bifidobacterium longum 之發酵優酪乳作為樣品，每日管餵 SD 大鼠低、中、高三個劑量之 NY 優酪乳凍乾粉末，各組劑量分別為 2.2 g/kg BW、4.4 g/kg BW 及 8.8 g/kg BW (相當於含優酪乳 9.3 g/kg BW、18.6 g/kg BW 及 37.2 g/kg BW; 含 B. longum 則各為 3.40 × 104 cfu/kg BW、6.80 × 104 cfu/kg BW 及 1.36 × 105 cfu/kg BW)，探討不同劑量對於腸道菌相及糞便情況之改善效果。研究結果顯示管餵低、中、高劑量之優酪乳隨著餵食時間增加，皆可以提升糞便 Bifidobacterium spp. 與 Lactobacillus spp. 之菌數及降低盲腸及糞便內 Enterobacteriaceae 及 Clostridium perfringens 之菌數，顯示其具有改善腸道菌相之功能。此外餵食 NY 優酪乳亦可以提升腸道轉運速率、降低糞便 pH 值、增加糞便水分含量，並可以提高糞便及盲腸內容物之短鏈脂肪酸含量，藉此增進腸道健康。綜合以上結果，含 B. longum 之發酵優酪乳可以透過調節腸道菌相、改善糞便情況及腸道蠕動速率，以及增加短鏈脂肪酸含量來達到促進腸道健康之功效。 | zh_TW |
| dc.description.abstract | Gut microbiota plays an important role in our body, it have several functions, such as immune regulation, blood lipid and blood glucose regulation, nutrients production and energy required for intestinal cells. And it provides a barrier to against pathogen invasion. Gut microbiota dysbiosis may lead diseases, as probiotics and fermented milk could rebalance gut microbiota in previous studies. In this study, using fermented yogurt containing Bifidobacterium longum as sample (NY yogurt) to understand the intestinal beneficial effect of different doses. Sprague-Dawley (SD) rats were orally administrated with 2.2 g/kg BW, 4.4 g/kg BW and 8.8 g/kg BW yogurt powder per day (i.e. 9.3 g/kg BW, 18.6 g/kg BW and 37.2 g/kg BW yogurt; 3.40 × 104 cfu/kg BW, 6.80 × 104 cfu/kg BW and 1.36 × 105 cfu/kg BW B. longum), respectively. This result shows that feeding B. longum contenting yogurt can increase fecal Bifidobacterium spp. and Lactobacillus spp. contents, and decrease the numbers of Enterobacteriaceae and Clostridium perfringens in cecum and feces. It displays that this yogurt has improvement effect on gut microbiota. In addition, feeding NY yogurt can increase intestinal transit rate, reduce fecal pH value, increase fecal moisture, and increase caecum and fecal short-chain fatty acid contents. Thereby, it shows the enhancing effects of intestinal health. Based on the above results, the fermented yogurt containing B. longum can improve fecal condition and intestinal peristalsis by adjusting gut microbiota, and enhancing the short-chain fatty acids to promote intestinal health. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T09:22:08Z (GMT). No. of bitstreams: 1 ntu-106-R04b22051-1.pdf: 2687406 bytes, checksum: d286c79954da3c43be186030480755a3 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 目錄
縮寫表…………………………………………………………………………………… I 中文摘要………………………………………………………………………………… II 英文摘要………………………………………………………………………………… III 目錄……………………………………………………………………………………… IV 圖目錄…………………………………………………………………………………… VIII 表目錄…………………………………………………………………………………… X 第一章 前言…………………………………………………………………….………. 1 第二章 文獻回顧………………………………………………………………….……. 2 一、 腸道菌相…………………………………………………………………………… 2 (一) 腸道菌相分布與組成………………………………………………………..….... 2 (二) 腸道菌相於胃腸道內之分布情形………………………………………….....…. 2 (三) 人體腸道菌相來源………………………………………………………..……… 4 (四) 腸道菌相對宿主之影響………..………………………………………..……….. 4 (五) 影響腸道菌相之因素…………………………………………………..………… 7 二、 腸道菌相失調導致之疾病………………………………………………………… 7 (一) 發炎性腸道疾病 (inflammatory bowel disease, IBD).…………….……………. 7 (二) 腸躁症 (irritable bowel syndrome, IBS)……………………………..…………... 7 (三) 特異性和過敏性疾病 (atopic and allergic diseases)…………………………..... 10 (四) 代謝症候群 (metabolic disease)…………………………………..……………. 10 三、 益生菌……………………………………………………………...………………. 11 (一) 益生菌於乳製品之應用………………………………………………...………... 11 (二) 乳酸桿菌 (Lactobacillus spp.) ……………………………………...…………… 12 (三) 雙歧桿菌 (Bifidobacterium spp.) ……………………………………...………… 12 (四) Bifidobacterium longum 之功效……………………………………...………….. 12 (五) 益生菌改善腸道菌相之策略……………………………………...……………... 14 四、 腸道有害菌……………………………………...…………………………………. 16 (一) 腸內菌科 (Enterobacteriaceae) ……………………………………...…………... 16 (二) 產氣莢膜梭菌 (Clostridium perfrigens) …………………………………...….. 17 五、 短鏈脂肪酸 (SCFAs)……………………………………...………………………. 17 (一) 乙酸……………………………………...……………………………………...… 18 (二) 丙酸……………………………………...……………………………………...… 18 (三) 丁酸……………………………………...……………………………………...… 18 六、 腸道菌相改善評估方法……………………………………...………….………… 20 第三章 研究動機與實驗架構………………………………………………………….. 21 一、 研究動機……………………………………...…………..……………………...… 21 二、 實驗架構……………………………………...…………………..……………...… 21 第四章 材料與方法…………………………………………………………………….. 23 一、 藥品試劑…………………………………………………………………………… 23 二、 儀器設備…………………………………………………………………………… 23 三、 試驗方法…………………………………………………………………………… 24 (一) 試驗物質……………………………………...…………………………………... 24 (二) 實驗動物飼養與照料……………………………………...…………………...… 30 (三) 實驗動物日程與動物犧牲及樣品採集……………………………………...…... 30 (四) 腸蠕動速率之測量……………………………………...………………………... 30 (五) 腸道菌相檢測……………………………………...……………………………... 34 (六) 糞便粒數計數及秤重……………………………………...……………………... 42 (七) 糞便水分含量之測定……………………………………...……………………... 42 (八) 糞便 pH 值之測定……………………………………...……………………...... 42 (九) 糞便及盲腸內容物短鏈脂肪酸測定……………………………………...……... 42 (十) 生物統計分析方法……………………………………...……………………....... 44 第五章 結果與討論…………………………………………………………………….. 51 一、 攝食量、攝水量與體重...………………………………………………………... 51 (一) 大鼠實驗期間攝食與攝水量變化情形……………………………………….…... 51 (二) 大鼠實驗期間體重變化情形……………………………………...………………. 51 二、 腸道菌相……………………………………...……………………....................... 51 (一) 糞便中 Clostridium perfringens 含量變化情形……………………….……..…... 51 (二) 糞便中 Enterobacteriaceae 含量變化情形………………………..………….…... 56 (三) 糞便中 Lactobacillus spp. 含量變化情形………………………………………... 56 (四) 糞便中 Bifidobacterium spp. 含量變化情形……………………………………... 59 (五) 盲腸內容物中菌相……………………………………...……………………......... 59 (六) 盲腸菌相與糞便菌相之比較……………………………………...………………. 59 三、 糞便情況……………………………………...………………..………................. 62 (一) 大鼠糞便之酸鹼值……………………………………...……………………......... 62 (二) 大鼠糞便水分含量………………………………...……………………................. 62 (三) 糞便數量及重量……………………………………...……………………............. 65 (四) 糞便情況改善作用…………………………………………...………………......... 65 四、 腸蠕動轉運速率……………………………………..…...……………………..... 65 五、 短鏈脂肪酸………………………………..………...…………………………..... 69 (一) 盲腸短鏈脂肪酸含量……………………………………...……………………..... 69 (二) 糞便短鏈脂肪酸含量……………………………………...……………………..... 69 (三) 盲腸與糞便短鏈脂肪酸含量比較……………………………………...…………. 69 (四) 短鏈脂肪酸與健康之關聯…………………………………….........……………... 72 六、 短鏈脂肪酸與腸道菌相之關聯……………………………..…………...………. 72 七、 短鏈脂肪酸與腸蠕動速率之關聯…………………………..……………...……. 72 八、 結果總整理……………………………………………………………………….. 75 第六章 結論……………………………………...……………………........................... 77 第七章 參考文獻……………………………………...……………………................... 79 | |
| dc.language.iso | zh-TW | |
| dc.title | Bifidobacterium longum 為主要成份優酪乳對於大鼠腸道菌相之改善效果 | zh_TW |
| dc.title | The improvement effect of yogurt containing mainly Bifidobacterium longum on gut microbiota in rats | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王志傑,邱秋霞,李俊霖,蔣慎思 | |
| dc.subject.keyword | Bifidobacterium longum,腸道菌相,腸道蠕動,短鏈脂肪酸, | zh_TW |
| dc.subject.keyword | Bifidobacterium longum,gut microbiota,intestinal peristalsis,short chain fatty acid, | en |
| dc.relation.page | 89 | |
| dc.identifier.doi | 10.6342/NTU201701001 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-06-27 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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