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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 廖憶純(Yi-Chun Liao) | |
| dc.contributor.author | Chia-Chung Chang | en |
| dc.contributor.author | 張佳鈞 | zh_TW |
| dc.date.accessioned | 2021-05-16T16:18:22Z | - |
| dc.date.available | 2018-08-23 | |
| dc.date.available | 2021-05-16T16:18:22Z | - |
| dc.date.copyright | 2013-08-23 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-15 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5922 | - |
| dc.description.abstract | EGFR signaling 是調控細胞遷移的訊息傳導路徑之一，而癌細胞中不正常活化的 EGFR signaling 會促進腫瘤的侵入性或癌細胞轉移。已知 EGFR signaling 可透過調控 focal adhesion 中 tensin 家族的 tensin3 及 Cten 蛋白質含量的消長，使蛋白質含量增加的 Cten 取代 tensin3，進而拆卸 actin fiber 增強乳癌細胞遷移的能力，且 EGF 誘導大量表現的 Cten 還可進一步延長 EGFR signaling 的活化時間，但 EGFR signaling 調控 Cten 表現上升的機制目前仍不清楚。本研究於 Cten 啟動子中並未發現與 EGF 調節有關的 cis-acting elements，且 EGF 的刺激也不會增加 Cten mRNA 之穩定性，進一步分析 Cten 啟動子上 nucleosome positioning 及 histone modification 等表觀基因調控方式，實驗結果發現 EGF 可促使 Cten 啟動子區域的 chromatin 結構變得較鬆散，並增加 Cten 啟動子上 histone tail 的 acetylation 修飾，而 histone acetyltransferase p300 的抑制劑 anacardic acid 可降低 EGF 誘導的 Cten 表現量，因此推測 EGF signaling 可能是藉由 histone acetyltrasnsferase p300 增加 Cten 啟動子區域 histone 的 acetylation 進而活化 Cten 基因的表現。 | zh_TW |
| dc.description.abstract | Epidermal growth factor receptor (EGFR) signaling is involved in regulation of cell migration. Aberrant activation of the pathway contributes to the tumor invasion and metastasis. Recent studies showed that EGF-driven breast cancer cells migration is mediated by a tensin3-Cten switch mechanism. EGF-induced up-regulation of Cten displaces tensin-3 from cytoplasmic tail of integrin and causes actin fiber disassembly then promotes cell migration. Furthermore, elevated expression of Cten by EGFR activation can prolong EGFR signaling. However, the molecular mechanism underlying EGFR signaling regulates the increased expression of Cten is still incompletely understood. Our results have shown that no EGF-responsive cis-acting elements were found within Cten promoter and Cten mRNA stability was not increased by EGF. Nonetheless, analyses of nuclesome positioning and histone modification within Cten promoter demonstrated that EGF stimulation increased the chromatin accessibility of Cten promoter and enhanced histone acetylation. Moreover, anacardic acid, a histone acetyltransferase p300 inhibitor, suppressed EGF-induced Cten expression, which suggested that the histone acetyltransferase p300 may be involved in the regulation of EGF-induced Cten expression. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-16T16:18:22Z (GMT). No. of bitstreams: 1 ntu-102-R00b22046-1.pdf: 2228579 bytes, checksum: fe1df28e9ec186538a328f72efe51377 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 目錄 I
縮寫表 IV 摘要 VI Abstract VII 一、本論文之研究基礎 1 1.1 表皮生長因子受體訊息傳導路徑 1 1.2 EGF 誘導 focal adhesion turnover 及細胞遷移機制 2 1.2.1 Focal adhesion 3 1.2.2 EGF 誘導 focal adhesion turnover 及細胞遷移之機制 4 1.3 Cten 於 EGFR signaling 中扮演的角色 5 1.3.1 Tensin 家族 5 1.3.2 Cten 6 1.3.3 Cten 於 EGFR signaling 中參與的調控機制 8 1.4 EGFR signaling調控基因表現的可能機制 9 1.4.1 EGF-responsive cis-acting elements 9 1.4.2 EGF 增加 mRNA 的穩定性 10 1.4.3 EGF 參與表觀基因調控 12 1.5 本論文之研究目的 15 二、材料與方法 16 2.1 實驗材料 16 2.1.1 菌種 16 2.1.2 質體 DNA 16 2.1.3 細胞株 18 2.1.4 細胞培養基 18 2.1.5 EGF 及抑制劑 18 2.1.6 抗體 18 2.2 實驗儀器設備 19 2.2.1 核酸電泳設備 19 2.2.2 蛋白質電泳設備 19 2.2.3 離心機 19 2.2.4 其他 19 2.3 實驗方法 20 2.3.1 細胞實驗 20 2.3.2 DNA 分析與質體之建構 23 2.3.3 RNA 分析 26 2.3.4 蛋白質分析 27 2.3.5 Dual luciferase assay 28 2.3.6 Chromatin accessibility by real-time PCR (CHART PCR) 28 2.3.7 染色質免疫沉澱 (Chromatin Immunoprecipitation assay, ChIP) 30 三、研究結果 33 3.1 選擇適用於探討 Cten 表現受 EGF調控之機制的細胞株 33 3.2 不同 EGF 濃度及處理時間對 RWPE-1細胞株中 Cten 表現的影響 33 3.3 EGF 於 RWPE-1 中藉由活化 ERK 進而調控 Cten 表現 34 3.4 於轉錄層次探討 Cten 受 EGF 誘導之機制 35 3.5 EGF 對 Cten mRNA 穩定性的影響 36 3.6 於表觀基因調控層次探討 Cten 受 EGF 誘導之機制 36 3.6.1 以 nuclesome positioning 探討 EGF 調控 Cten 表現之機制 37 3.6.2 以 histone modification 探討 EGF 調控 Cten 表現之機制 37 3.7 用癌症細胞株 HeLa 及 DU145探討 Cten 受 EGF signaling 誘導之調控機制 39 3.8 利用染色質免疫沉澱法探討細胞株 HeLa 中 Cten 受 EGF signaling 誘導之分子機制 40 四、討論與未來研究方向 42 五、參考文獻 49 六、圖與表 57 圖 3.1 不同細胞株中 Cten 表現量受 EGF 影響之分析 58 圖 3.2 不同 EGF 濃度及處理時間對 RWPE-1 細胞株中 Cten 表現量的影響 59 圖 3.3 MEK inhibitor U0126 對 RWPE-1 細胞株中 EGF 誘導之 Cten 蛋白質含量及 ERK 磷酸化之影響 60 圖 3.4 Cten 啟動子活性受 EGF 影響之分析 61 圖 3.5 EGF對 Cten mRNA 穩定性的影響 62 圖3.6 EGF 於 RWPE-1 細胞株中對 Cten 啟動子受 MNase 之 accessibility 的影響。 64 圖 3.7 HAT P300 inhibitor anacardic acid (AA) 及 HDAC inhibitor TSA 對 EGF 誘導之 Cten 表現量的影響 65 圖3.8 Anacardic acid 可抑制 EGF 所誘導之 Cten mRNA 轉錄而不影響 p300 mRNA 66 圖 3.10 TSA 可促進 HeLa cells 中 Cten mRNA 之表現量 68 圖3.11 MEK inhibitor U0126 對 HeLa 細胞株中 EGF 誘導之 Cten 蛋白質含量及 ERK 磷酸化之影響 69 圖3.12 HeLa 細胞株中 Cten 啟動子活性受 EGF 影響之分析 70 圖3.13 利用 ENCODE 資料庫分析 Cten 基因中具有的 histone acetylation 位置及轉錄因子結合位。 71 圖3.14 以 ChIP assay 分析 Cten 啟動子之 histone acetylation 受 EGF 之影響 72 表一 73 表二 74 表三、表四、表五 75 | |
| dc.language.iso | zh-TW | |
| dc.subject | 表觀基因調控機制 | zh_TW |
| dc.subject | 細胞遷移 | zh_TW |
| dc.subject | 集中點附著體 | zh_TW |
| dc.subject | 基因轉錄 | zh_TW |
| dc.subject | 表皮生長因子 | zh_TW |
| dc.subject | Trichostatin A | en |
| dc.subject | EGF | en |
| dc.subject | EGFR signaling | en |
| dc.subject | focal adhesion | en |
| dc.subject | Tensin | en |
| dc.subject | Cten | en |
| dc.subject | U0126 | en |
| dc.subject | cis-acting element | en |
| dc.subject | RNA stability | en |
| dc.subject | epigenetic regulation | en |
| dc.subject | histone modification | en |
| dc.subject | Anacardic acid | en |
| dc.title | 表皮生長因子促進 Cten 基因表現之機制探討 | zh_TW |
| dc.title | Elucidating the Mechanism of EGF-Induced Cten Gene Expression | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王愛玉(Ai-Yu Wang),張麗冠(Li-Kwan Chang),謝淑貞(Shu-Chen Hsieh),賴韻如(Yun-Ju Lai) | |
| dc.subject.keyword | 表皮生長因子,細胞遷移,集中點附著體,基因轉錄,表觀基因調控機制, | zh_TW |
| dc.subject.keyword | EGF,EGFR signaling,focal adhesion,Tensin,Cten,U0126,cis-acting element,RNA stability,epigenetic regulation,histone modification,Trichostatin A,Anacardic acid, | en |
| dc.relation.page | 76 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2013-08-15 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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