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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李昆達(Kung-Ta Lee) | |
dc.contributor.author | Chien-Hsun Tseng | en |
dc.contributor.author | 曾建勳 | zh_TW |
dc.date.accessioned | 2021-06-16T09:16:01Z | - |
dc.date.available | 2022-07-20 | |
dc.date.copyright | 2017-07-20 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-07-17 | |
dc.identifier.citation | Alberto Benedetti, Simona Daly, Hermes Pagani, and Roberto Xaiz. 2009. 'European Patent 20070154998'.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59099 | - |
dc.description.abstract | 維生素K化合物,尤其是維生素K2 (MK-7) ,已被證實可以改善心血管疾病、骨質疏鬆等疾病。多篇研究指出,MK-7的功能性、半衰期、吸收率及體內利用率皆較其它維生素K優異,但在自然界中MK-7含量低,僅在少量發酵食品中可以獲得,例如納豆,是目前世界上MK-7含量最多的食物,而MK-7主要存在於納豆菌 (Bacillus subtilis natto) 的細胞膜上。從本實驗室所收集的20株納豆菌中篩選出具有生產MK-7潛力的納豆菌NTU-19,再利用前人研究之生產MK-7最佳化培養基配方進行改良,經搖瓶培養改良培養基配方,再放大至5公升生物反應器中進行培養。經過培養基最佳化,搖瓶培養120小時後,可生產MK-7濃度36.68 mg/L。在生物反應器放大培養中,本研究採用冷藏方式,發現可以使MK-7濃度提早達到高點,使培養時間縮短將近一半,是未被報導過的培養方式。目前,生物反應器批次培養120小時,MK-7濃度為37.12 mg/L,而饋料培養120小時則可以將產量提高1.5倍,濃度53.89 mg/L。 | zh_TW |
dc.description.abstract | The Vitamin K series, particularly Vitamin K2 (MK-7), has been confirmed that can reduce both cardiovascular disease and prevent osteoporosis. Dozens of scientific reports have shown that MK-7 has the best bioavailability, half-life, absorption rate, and function in the human body. However, MK-7 can only be found in some fermented foods, such as Natto, which is the traditional fermented soy bean food in Japan. Previous reports show that Natto has the most abundant MK-7 in the world. The MK-7 in the Natto is synthesized by Bacillus subtilis natto. Bacillus subtilis strain NTU-19 which was selected from 20 strains of Bacillus subtilis natto collected in our laboratory. NTU-19 shows the most MK-7 production potential. Then, we adjust the medium for MK-7 production based on previous report; make it more suitable to NTU-19 for MK-7 production. In Hinton flask culture, NTU-19 produced MK-7 concentration to 36.68 mg/L after 120 hours at 37oC, 125 rpm. In 5-L bioreactor batch culture, the NTU-19 produced MK-7 concentration to 37.12 mg/L. Finally, in 5-L bioreactor fed-batch culture, NTU-19 produced MK-7 concentration to 53.89 mg/L after 120 hours, the total MK-7 concentration increase 1.5- fold during fermentation. Moreover, in this study, we found the special cold effect that can reduce almost half time of culture. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:16:01Z (GMT). No. of bitstreams: 1 ntu-106-R04b22040-1.pdf: 2097704 bytes, checksum: d2062c06900850124aed3b64723b0993 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii 目錄 iv 圖目錄 vii 表目錄 viii 壹、 前言 1 1.1維生素K 1 1.1.1維生素K簡介 1 1.1.2維生素K生理功能 3 1.1.3維生素K2 (MK-7) 的重要性 4 1.1.4 MK-7生產現況 6 1.2 納豆與納豆菌 7 1.2.1納豆食品起源 7 1.2.2納豆菌 (Bacillus subtilis natto) 8 1.3 研究目的 10 1.4 研究架構 11 貳、 材料與方法 12 2.1菌株來源 12 2.2菌種活化與篩選 12 2.3 Hinton氏三角瓶振盪培養 12 2.4 Hinton氏三角瓶靜置培養 12 2.5培養基濃度最佳化策略 13 2.6 5公升發酵槽培養設備及各項參數設定 13 2.7 MK-7萃取方法 14 2.8 MK-7分析方法 14 2.9 內孢子染色 15 參、 結果與討論 16 3.1納豆菌MK-7含量比較篩選結果 16 3.1.1試管篩選結果 16 3.1.2 Hinton氏三角瓶篩選結果 17 3.2 NTU-19 培養條件探討 18 3.2.1溫度對NTU-19 MK-7濃度之探討 18 3.2.2振盪培養與靜置培養對NTU-19生產 MK-7之影響 19 3.2.3不同碳、氮源對NTU-19 生產MK-7影響之探討 19 3.3最適培養基成分探討 21 3.3.1關鍵氮源確認 21 3.3.2不同碳氮比例對MK-7產量影響 22 3.3.3相同碳氮比例於不同濃度對MK-7濃度之影響 23 3.4 5公升發酵槽培養 24 3.4.1批次培養 (一) 24 3.4.2批次培養 (二) 25 3.4.3饋料培養 (一) -pH-stat策略 27 3.4.4饋料培養 (二) -定速饋料策略 28 肆、 結論 29 4.1結論 29 4.2未來展望 31 伍、 圖表 32 陸、 參考資料 51 柒、 附錄 56 | |
dc.language.iso | zh-TW | |
dc.title | 以納豆菌生產維生素K2 (MK-7) 之研究 | zh_TW |
dc.title | Production of Vitamin K2 (MK-7) from Bacillus subtilis natto | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉啟德(Chi-Te Liu),楊健志(Chien-Chih Yang) | |
dc.subject.keyword | MK-7,維生素K2,饋料培養,納豆菌,發酵槽, | zh_TW |
dc.subject.keyword | MK-7,Vitamin K2,Fed-batch,Bacillus subtilis natto,Bioreactor, | en |
dc.relation.page | 60 | |
dc.identifier.doi | 10.6342/NTU201701623 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-07-17 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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