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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58987完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 朱士維(Si Wei Chu) | |
| dc.contributor.author | Yen-Jun Huang | en |
| dc.contributor.author | 黃彥鈞 | zh_TW |
| dc.date.accessioned | 2021-06-16T08:42:54Z | - |
| dc.date.available | 2015-09-07 | |
| dc.date.copyright | 2013-09-07 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-28 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58987 | - |
| dc.description.abstract | 電驅動奈米流道已經被廣泛的利用在許多生化分析的系統上，尤其在尺度更微小、因而較難以控制的蛋白質分析上。雖然人們對蛋白質本身的生化結構已有深入的認知，然而許多蛋白質如何在奈米流道的行為仍是未知，例如，蛋白質如何吸附道流道表面上，和蛋白質如何影響流道的電滲流(electro-osmotic flow)傳輸。這份研究利用電流監控法(current-monitoring method)來量測奈米微流道(高度約500奈米)中電滲流移動率(electro-osmotic mobility)在不同濃度的牛血清白蛋白(bovine serum albumin: BSA)下的表現。我們發現電滲流移動率會隨著蛋白質濃度的增加而下降，此下降趨勢並非無限制延伸到零，而是達到一個飽和值。藉由和隨機序列吸附模型(random-sequential-adsorption model)的指數函數比對下，可以得到造成移動率變化的特徵濃度為1.4±0.3mg/ml，而移動率的飽和值為1.3±0.1 cm*um/V s. | zh_TW |
| dc.description.abstract | Electrokinetically-driven nanofluidics has been widely used in micro-total-analysis system (μTAS), especially for proteomic analysis. However, many details in how proteins were absorbed onto the surface and perturbed the electro-osmotic flow (EOF) are still elusive. In this study, we investigated the reliability of current-monitoring method by reproducing the results under different conditions, and used this method to measure electro-osmotic mobility under different concentration of bovine serum albumin (BSA) in nano-slits (channel height ~ 500nm). We observed that EOF mobility would be reduced as BSA concentration increases, and reach a saturation level at high BSA concentration. By fitting with exponential decay from random-sequential-adsorption model suggested by Adamczyk et al1,2, two relevant parameters were found: characteristic perturbing concentration is 1.4±0.3 mg/ml and mobility at high concentration is 1.3±0.1 um*cm/V s. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T08:42:54Z (GMT). No. of bitstreams: 1 ntu-102-R00245015-1.pdf: 4141721 bytes, checksum: e43d1010e55a46bd29f0a079f34b9190 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | Chapter 1 Introduction 11
1.1 Miniaturized fluidics for micro analysis 11 1.2 Fluid transport in micro/nano-channels 13 1.3 Why understand protein adsorption in microfluidics? 17 Chapter 2 Theory of electrokinetics in nanoslits 21 2.1 Electrical double layer-the Debye length 21 2.2 Current-monitoring method 23 2.3 The concentration-dependent zeta potential: Exponential decay from random-sequential-adsorption (RSA) model 25 Chapter 3 Experimental materials and method: 27 3.1 Sample preparation 27 3.2 Device fabrication: 27 3.2.1 Photolithography 27 3.2.2 Loading holes 28 3.2.3 PSQ bonding process 29 3.3 Experimental setup for the current-monitoring methods 30 3.4 Data analysis 31 Chapter 4 Results and discussions 33 4.1 Basic calibrations: Multi-meter and electrode polarization 33 4.2 Reliability test of current-monitoring method 34 4.2.1 Reproducibility with fresh solution in used channels 34 4.2.2 Reproducibility with fresh solution and new channels 35 4.2.3 Reproducibility with the same solution by switching electric field 36 4.3 Effect of ionic concentration on EOF mobility 38 4.4 Effect of protein adsorption on EOF mobility 39 4.4.1 Screening effect of surface charge due to adsorbed proteins 39 4.4.2 Dependence of EOF mobility on profiles of adsorbed proteins: side-on or aggregate manners 44 Chapter 5 Conclusion and future perspective 46 References 48 | |
| dc.language.iso | en | |
| dc.subject | 微流體 | zh_TW |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | 電滲流 | zh_TW |
| dc.subject | 牛血清蛋白 | zh_TW |
| dc.subject | electroosmosis | en |
| dc.subject | microfluidics | en |
| dc.subject | biosensor | en |
| dc.subject | bovine serum albumin | en |
| dc.title | 探討在奈米微流道中蛋白質吸附對電滲流的影響 | zh_TW |
| dc.title | Effect of protein adsorption on electro-osmotic flow in nano-slits | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 周家復(Chia Fu Chou) | |
| dc.contributor.oralexamcommittee | 趙玲(Ling Chao) | |
| dc.subject.keyword | 微流體,生物感測器,電滲流,牛血清蛋白, | zh_TW |
| dc.subject.keyword | microfluidics,biosensor,electroosmosis,bovine serum albumin, | en |
| dc.relation.page | 52 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-28 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 應用物理所 | zh_TW |
| 顯示於系所單位： | 應用物理研究所 | |
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