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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄧述諄(Shu-Chun Teng) | |
dc.contributor.author | Li Kao | en |
dc.contributor.author | 高立 | zh_TW |
dc.date.accessioned | 2021-06-16T08:28:31Z | - |
dc.date.available | 2014-02-25 | |
dc.date.copyright | 2014-02-25 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-01-14 | |
dc.identifier.citation | Akiyoshi, B., and Biggins, S. (2010). Cdc14-dependent dephosphorylation of a kinetochore protein prior to anaphase in Saccharomyces cerevisiae. Genetics 186, 1487-1491.
Allen, J.B., Zhou, Z., Siede, W., Friedberg, E.C., and Elledge, S.J. (1994). The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. Genes Dev 8, 2401-2415. Altman, R., and Kellogg, D. (1997). Control of mitotic events by Nap1 and the Gin4 kinase. J Cell Biol 138, 119-130. Asano, S., Park, J.E., Sakchaisri, K., Yu, L.R., Song, S., Supavilai, P., Veenstra, T.D., and Lee, K.S. (2005). Concerted mechanism of Swe1/Wee1 regulation by multiple kinases in budding yeast. EMBO J 24, 2194-2204. Bailly, E., Cabantous, S., Sondaz, D., Bernadac, A., and Simon, M.N. (2003). Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci 116, 4119-4130. Bardin, A.J., and Amon, A. (2001). Men and sin: what's the difference? Nat Rev Mol Cell Biol 2, 815-826. Bardin, A.J., Visintin, R., and Amon, A. (2000). A mechanism for coupling exit from mitosis to partitioning of the nucleus. Cell 102, 21-31. Bembenek, J., Kang, J., Kurischko, C., Li, B., Raab, J.R., Belanger, K.D., Luca, F.C., and Yu, H. (2005). Crm1-mediated nuclear export of Cdc14 is required for the completion of cytokinesis in budding yeast. Cell Cycle 4, 961-971. Bilsland, E., Hult, M., Bell, S.D., Sunnerhagen, P., and Downs, J.A. (2007). The Bre5/Ubp3 ubiquitin protease complex from budding yeast contributes to the cellular response to DNA damage. DNA repair 6, 1471-1484. Bloom, J., Cristea, I.M., Procko, A.L., Lubkov, V., Chait, B.T., Snyder, M., and Cross, F.R. (2011). Global analysis of Cdc14 phosphatase reveals diverse roles in mitotic processes. J Biol Chem 286, 5434-5445. Booher, R.N., Deshaies, R.J., and Kirschner, M.W. (1993). Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J 12, 3417-3426. Boyle, E.I., Weng, S., Gollub, J., Jin, H., Botstein, D., Cherry, J.M., and Sherlock, G. (2004). GO::TermFinder--open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes. Bioinformatics 20, 3710-3715. Brachmann, C.B., Davies, A., Cost, G.J., Caputo, E., Li, J., Hieter, P., and Boeke, J.D. (1998). Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14, 115-132. Breitkreutz, A., Choi, H., Sharom, J.R., Boucher, L., Neduva, V., Larsen, B., Lin, Z.Y., Breitkreutz, B.J., Stark, C., Liu, G., et al. (2010). A global protein kinase and phosphatase interaction network in yeast. Science 328, 1043-1046. Chant, J., and Herskowitz, I. (1991). Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway. Cell 65, 1203-1212. Chant, J., Mischke, M., Mitchell, E., Herskowitz, I., and Pringle, J.R. (1995). Role of Bud3p in producing the axial budding pattern of yeast. J Cell Biol 129, 767-778. Cheeseman, I.M., Anderson, S., Jwa, M., Green, E.M., Kang, J., Yates, J.R., 3rd, Chan, C.S., Drubin, D.G., and Barnes, G. (2002). Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111, 163-172. Eluere, R., Offner, N., Varlet, I., Motteux, O., Signon, L., Picard, A., Bailly, E., and Simon, M.N. (2007). Compartmentalization of the functions and regulation of the mitotic cyclin Clb2 in S. cerevisiae. J Cell Sci 120, 702-711. Fitzpatrick, P.J., Toyn, J.H., Millar, J.B., and Johnston, L.H. (1998). DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase. Mol Gen Genet 258, 437-441. Freeman, L., Aragon-Alcaide, L., and Strunnikov, A. (2000). The condensin complex governs chromosome condensation and mitotic transmission of rDNA. J Cell Biol 149, 811-824. Geymonat, M., Spanos, A., Wells, G.P., Smerdon, S.J., and Sedgwick, S.G. (2004). Clb6/Cdc28 and Cdc14 regulate phosphorylation status and cellular localization of Swi6. Mol Cell Biol 24, 2277-2285. Guacci, V., Hogan, E., and Koshland, D. (1994). Chromosome condensation and sister chromatid pairing in budding yeast. J Cell Biol 125, 517-530. Guarente, L. (1983). Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast. Methods in enzymology 101, 181-191. Haase, S.B., Winey, M., and Reed, S.I. (2001). Multi-step control of spindle pole body duplication by cyclin-dependent kinase. Nat Cell Biol 3, 38-42. Hall, M.C., Jeong, D.E., Henderson, J.T., Choi, E., Bremmer, S.C., Iliuk, A.B., and Charbonneau, H. (2008). Cdc28 and Cdc14 control stability of the anaphase-promoting complex inhibitor Acm1. J Biol Chem 283, 10396-10407. Han, C.L., Chien, C.W., Chen, W.C., Chen, Y.R., Wu, C.P., Li, H., and Chen, Y.J. (2008). A multiplexed quantitative strategy for membrane proteomics: opportunities for mining therapeutic targets for autosomal dominant polycystic kidney disease. Mol Cell Proteomics 7, 1983-1997. Hirano, T. (1999). SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates? Genes Dev 13, 11-19. Hirano, T., and Mitchison, T.J. (1994). A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro. Cell 79, 449-458. Jaspersen, S.L., Charles, J.F., and Morgan, D.O. (1999). Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14. Curr Biol 9, 227-236. Jaspersen, S.L., Charles, J.F., Tinker-Kulberg, R.L., and Morgan, D.O. (1998). A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae. Mol Biol Cell 9, 2803-2817. Jaspersen, S.L., and Morgan, D.O. (2000). Cdc14 activates cdc15 to promote mitotic exit in budding yeast. Curr Biol 10, 615-618. Khmelinskii, A., Lawrence, C., Roostalu, J., and Schiebel, E. (2007). Cdc14-regulated midzone assembly controls anaphase B. J Cell Biol 177, 981-993. Knop, M., Siegers, K., Pereira, G., Zachariae, W., Winsor, B., Nasmyth, K., and Schiebel, E. (1999). Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast 15, 963-972. Koshland, D., and Strunnikov, A. (1996). Mitotic chromosome condensation. Annual review of cell and developmental biology 12, 305-333. Lavoie, B.D., Hogan, E., and Koshland, D. (2002). In vivo dissection of the chromosome condensation machinery: reversibility of condensation distinguishes contributions of condensin and cohesin. J Cell Biol 156, 805-815. Lavoie, B.D., Tuffo, K.M., Oh, S., Koshland, D., and Holm, C. (2000). Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren. Mol Biol Cell 11, 1293-1304. Lew, D.J., and Reed, S.I. (1995a). A cell cycle checkpoint monitors cell morphogenesis in budding yeast. J Cell Biol 129, 739-749. Lew, D.J., and Reed, S.I. (1995b). Cell cycle control of morphogenesis in budding yeast. Current opinion in genetics & development 5, 17-23. Li, C.R., Yong, J.Y., Wang, Y.M., and Wang, Y. (2012). CDK regulates septin organization through cell-cycle-dependent phosphorylation of the Nim1-related kinase Gin4. J Cell Sci 125, 2533-2543. Liang, C., and Stillman, B. (1997). Persistent initiation of DNA replication and chromatin-bound MCM proteins during the cell cycle in cdc6 mutants. Genes Dev 11, 3375-3386. Longtine, M.S., Theesfeld, C.L., McMillan, J.N., Weaver, E., Pringle, J.R., and Lew, D.J. (2000). Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol Cell Biol 20, 4049-4061. Lorincz, A.T., and Reed, S.I. (1986). Sequence analysis of temperature-sensitive mutations in the Saccharomyces cerevisiae gene CDC28. Mol Cell Biol 6, 4099-4103. Lu, X., and Zhu, H. (2005). Tube-gel digestion: a novel proteomic approach for high throughput analysis of membrane proteins. Mol Cell Proteomics 4, 1948-1958. Miller, J.H. (1972). Experiments in molecular genetics (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory). Mocciaro, A., and Schiebel, E. (2010). Cdc14: a highly conserved family of phosphatases with non-conserved functions? J Cell Sci 123, 2867-2876. Montagnoli, A., Valsasina, B., Brotherton, D., Troiani, S., Rainoldi, S., Tenca, P., Molinari, A., and Santocanale, C. (2006). Identification of Mcm2 phosphorylation sites by S-phase-regulating kinases. J Biol Chem 281, 10281-10290. Moritani, M., and Ishimi, Y. (2013). Inhibition of DNA binding of MCM2-7 complex by phosphorylation with cyclin-dependent kinases. Journal of biochemistry 154, 363-372. Olsen, J.V., de Godoy, L.M., Li, G., Macek, B., Mortensen, P., Pesch, R., Makarov, A., Lange, O., Horning, S., and Mann, M. (2005). Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap. Mol Cell Proteomics 4, 2010-2021. Ouspenski, II, Cabello, O.A., and Brinkley, B.R. (2000). Chromosome condensation factor Brn1p is required for chromatid separation in mitosis. Mol Biol Cell 11, 1305-1313. Peters, J.M. (2002). The anaphase-promoting complex: proteolysis in mitosis and beyond. Mol Cell 9, 931-943. Pringle, J.R. (1991). Staining of bud scars and other cell wall chitin with calcofluor. Methods in enzymology 194, 732-735. Sanders, S.L., and Herskowitz, I. (1996). The BUD4 protein of yeast, required for axial budding, is localized to the mother/BUD neck in a cell cycle-dependent manner. J Cell Biol 134, 413-427. Schneider, B.L., Seufert, W., Steiner, B., Yang, Q.H., and Futcher, A.B. (1995). Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiae. Yeast 11, 1265-1274. Shou, W., Seol, J.H., Shevchenko, A., Baskerville, C., Moazed, D., Chen, Z.W., Jang, J., Charbonneau, H., and Deshaies, R.J. (1999). Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97, 233-244. Skoufias, D.A., Indorato, R.L., Lacroix, F., Panopoulos, A., and Margolis, R.L. (2007). Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed. J Cell Biol 179, 671-685. Stegmeier, F., and Amon, A. (2004). Closing mitosis: the functions of the Cdc14 phosphatase and its regulation. Annu Rev Genet 38, 203-232. Stegmeier, F., Visintin, R., and Amon, A. (2002). Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell 108, 207-220. Strunnikov, A.V., Hogan, E., and Koshland, D. (1995). SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family. Genes Dev 9, 587-599. Strunnikov, A.V., and Jessberger, R. (1999). Structural maintenance of chromosomes (SMC) proteins: conserved molecular properties for multiple biological functions. European journal of biochemistry / FEBS 263, 6-13. Sutani, T., Yuasa, T., Tomonaga, T., Dohmae, N., Takio, K., and Yanagida, M. (1999). Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4. Genes Dev 13, 2271-2283. Tanaka, T.U., Stark, M.J., and Tanaka, K. (2005). Kinetochore capture and bi-orientation on the mitotic spindle. Nat Rev Mol Cell Biol 6, 929-942. Taylor, G.S., Liu, Y., Baskerville, C., and Charbonneau, H. (1997). The activity of Cdc14p, an oligomeric dual specificity protein phosphatase from Saccharomyces cerevisiae, is required for cell cycle progression. J Biol Chem 272, 24054-24063. Tsai, C.F., Wang, Y.T., Chen, Y.R., Lai, C.Y., Lin, P.Y., Pan, K.T., Chen, J.Y., Khoo, K.H., and Chen, Y.J. (2008). Immobilized metal affinity chromatography revisited: pH/acid control toward high selectivity in phosphoproteomics. Journal of proteome research 7, 4058-4069. Tseng, S.F., Lin, J.J., and Teng, S.C. (2006). The telomerase-recruitment domain of the telomere binding protein Cdc13 is regulated by Mec1p/Tel1p-dependent phosphorylation. Nucleic Acids Res 34, 6327-6336. Tseng, S.F., Shen, Z.J., Tsai, H.J., Lin, Y.H., and Teng, S.C. (2009). Rapid Cdc13 turnover and telomere length homeostasis are controlled by Cdk1-mediated phosphorylation of Cdc13. Nucleic Acids Res 37, 3602-3611. Tsou, C.C., Tsai, C.F., Tsui, Y.H., Sudhir, P.R., Wang, Y.T., Chen, Y.J., Chen, J.Y., Sung, T.Y., and Hsu, W.L. (2010). IDEAL-Q, an automated tool for label-free quantitation analysis using an efficient peptide alignment approach and spectral data validation. Mol Cell Proteomics 9, 131-144. Ubersax, J.A., Woodbury, E.L., Quang, P.N., Paraz, M., Blethrow, J.D., Shah, K., Shokat, K.M., and Morgan, D.O. (2003). Targets of the cyclin-dependent kinase Cdk1. Nature 425, 859-864. Uhlmann, F., Lottspeich, F., and Nasmyth, K. (1999). Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1. Nature 400, 37-42. Uhlmann, F., Wernic, D., Poupart, M.A., Koonin, E.V., and Nasmyth, K. (2000). Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast. Cell 103, 375-386. Varela, E., Shimada, K., Laroche, T., Leroy, D., and Gasser, S.M. (2009). Lte1, Cdc14 and MEN-controlled Cdk inactivation in yeast coordinate rDNA decompaction with late telophase progression. EMBO J 28, 1562-1575. Vas, A.C., Andrews, C.A., Kirkland Matesky, K., and Clarke, D.J. (2007). In vivo analysis of chromosome condensation in Saccharomyces cerevisiae. Mol Biol Cell 18, 557-568. Visintin, R., Craig, K., Hwang, E.S., Prinz, S., Tyers, M., and Amon, A. (1998). The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation. Mol Cell 2, 709-718. Visintin, R., Hwang, E.S., and Amon, A. (1999). Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature 398, 818-823. Vizcaino, J.A., Cote, R.G., Csordas, A., Dianes, J.A., Fabregat, A., Foster, J.M., Griss, J., Alpi, E., Birim, M., Contell, J., et al. (2013). The PRoteomics IDEntifications (PRIDE) database and associated tools: status in 2013. Nucleic Acids Res 41, D1063-1069. Wang, Y.T., Tsai, C.F., Hong, T.C., Tsou, C.C., Lin, P.Y., Pan, S.H., Hong, T.M., Yang, P.C., Sung, T.Y., Hsu, W.L., et al. (2010). An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. Journal of proteome research 9, 5582-5597. Xu, H., and Freitas, M.A. (2009). MassMatrix: a database search program for rapid characterization of proteins and peptides from tandem mass spectrometry data. Proteomics 9, 1548-1555. Xu, H., Hsu, P.H., Zhang, L., Tsai, M.D., and Freitas, M.A. (2010). Database search algorithm for identification of intact cross-links in proteins and peptides using tandem mass spectrometry. Journal of proteome research 9, 3384-3393. Yoshida, S., Kono, K., Lowery, D.M., Bartolini, S., Yaffe, M.B., Ohya, Y., and Pellman, D. (2006). Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. Science 313, 108-111. Zachariae, W., and Nasmyth, K. (1999). Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13, 2039-2058. Zhai, Y., Yung, P.Y., Huo, L., and Liang, C. (2010). Cdc14p resets the competency of replication licensing by dephosphorylating multiple initiation proteins during mitotic exit in budding yeast. J Cell Sci 123, 3933-3943. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58741 | - |
dc.description.abstract | 酵母菌的細胞週期調控已經被完全的研究岀其機制,而負責調控的磷酸酶Cdk1利用與不同的週期素結合,以在不同細胞週期時對不同的受質進行磷酸化,以利細胞完成細胞週期。
Cdc14已知會針對被Cdk1磷酸化的受質進行去磷酸化,並調控M phase的週期素的降解作用,以利細胞可以離開M phase進行下一個細胞週期,Cdc14 在這個調控中是一個必要的去磷酸酶,更是細胞負責調控離開M phase的FEAR (cdc fourteen early anaphase release) 及MEN (mitotic exit network)中不可或缺的一個酵素。而Cdc14主要的功能在於將Cdh1/APC/C complex去磷酸並活化,進而使得M phase的週期素降解。但其他與有絲分裂及細胞分裂由Cdc14調控的受質並不清楚。我們利用質譜分析的方法對酵母菌來進行系統分析以找出Cdc14所調控的受質與位點。利用質譜分析,我們找到了455個可能的受質共835個位點。 我們進一步發現Cdc14可對被Cdk1磷酸化的受質進行去磷酸化來參予調控細胞週期。Cdc14可以經由Smc4 S128的位點去磷酸化調控染色體縮合(condensation)及有絲分裂進行,及對Bud3 S1549 與T1566的位點去磷酸化而調控Clb2在細胞內的位置及胞質分裂的進行。這些結果讓我們對於Cdc14在調控細胞離開M phase 所扮演的角色有更進一步的了解。 | zh_TW |
dc.description.abstract | Degradation of the M phase cyclins triggers the exit from M phase. Cdc14 is the major phosphatase required for the exit of the M phase. One of the functions of Cdc14 is to dephosphorylate and activate the Cdh1/APC/C complex, resulting in the degradation of the M phase cyclins. However, other crucial targets of Cdc14 for mitosis and cytokinesis remain to be elucidated. Here we systematically analyzed the positions of dephosphorylation sites for the Cdc14 in the budding yeast Saccharomyces cerevisiae. Quantitative mass spectrometry identified a total of 835 dephosphorylation sites on 455 potential Cdc14 substrates in vivo.
We validated two candidates that phosphorylated by Cdk1 and dephosphorylated by Cdc14. Cdc14 promotes the process of chromosome condensation via regulated the phosphorylated status of Smc4 on S128, and affects the bud-neck localization of Clb2 via regulated the phosphorylated status of Bud3 on S1549 and T1566. From these results we discovered that Cdc14-mediated dephosphorylation of Smc4 and Bud3 is essential for proper mitosis and cytokinesis, respectively. These results provide insight into the Cdc14-mediated pathways for exiting of M phase. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T08:28:31Z (GMT). No. of bitstreams: 1 ntu-103-D92b41008-1.pdf: 2128162 bytes, checksum: 652419124525da308b4ea1a1eac40597 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 口試委員會審定書 …………………………………………………………………………………………… i
誌謝 …………………………………………………………………………………………………………………. ii 中文摘要 …………………………………………………………………………………………………………… iii Abstract ……………………………………………………………………………………………………………. iv Introduction ……………………………………………………………………………………………………….. 1 Materials and Methods ………………………………………………………………………………………… 5 Yeast Strains and Plasmids ……………………………………………………………………………… 5 Gel-assisted Digestion …………………………………………………………………………………… 6 Immobilized Metal Affinity Chromatography (IMAC) Procedure …………………… 6 LC-MS/MS Analysis ……………………………………………………………………………………… 7 Database Search ……………………………………………………………………………………………… 8 Quantitative Analysis by IDEAL-Q ………………………………………………………………… 9 Gene Ontology Analysis ………………………………………………………………………………… 10 Generation of Phospho-specific Antibodies …………………………………………………… 11 Protein analysis ……………………………………………………………………………………………… 11 In vitro kinase and phosphatase assays …………………………………………………………… 12 Yeast Two hybrid …………………………………………………………………………………………… 13 Quantitative Fluorescence Microscopy …………………………………………………………… 13 Immunoprecipitation ……………………………………………………………………………………… 14 Chromatin Fractionation ………………………………………………………………………………… 14 PART I: Results of LC-MS/MS Large-scale identification of potential substrates of Cdc14 and their dephosphorylation sites ……………………………………………………………………………………………………………………… 16 PART II: The regulation of Cdc14 in mitosis The roles of Smc4 ………………………………………………………………………………………… 18 Smc4 is phosphorylated by Cdk1 and dephosphorylated by Cdc14 ……………………………………………………………… 19 Phosphorylation of Smc4 at S128 is required for chromosome condensation and chromosome segregation ……………………………………………………………………………… 20 PART III: The regulation of Cdc14 in cytokinesis The roles of Bud3 …………………………………………………………………………………………… 22 Bud3 is phosphorylated by Cdk1 and dephopshorylated by Cdc14 ……………………………………………………………… 24 Phosphorylation of Bud3 at S1549 and T1566 is required for Clb2 recruitment and Swe1 degradation ………………………………………………………………………………………… 25 Discussion …………………………………………………………………………………………………………… 28 References …………………………………………………………………………………………………………… 32 | |
dc.language.iso | en | |
dc.title | 利用質譜分析的方法研究Cdc14調控細胞有絲分裂與胞質分裂的機制 | zh_TW |
dc.title | Global analysis of Cdc14 dephosphorylation sites reveals essential regulatory role in mitosis and cytokinesis | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 鄭子豪(Tzu-Hao Cheng),李財坤(Tsai-Kun Li),林敬哲(Jing-Jer Lin),王萬波(Won-Bo Wang) | |
dc.subject.keyword | Cdc14,磷酸化,有絲分裂,細胞分裂,Smc4,Bud3, | zh_TW |
dc.subject.keyword | Cdc14,phosphorylation,mitosis,cytokinesis,Smc4,Bud3, | en |
dc.relation.page | 81 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-01-14 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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