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標題: | 阿拉伯芥C2-domain ABA-related protein family (CAR) 在光與茉莉酸訊息傳遞中之功能性探討 Functional studies of Arabidopsis C2-domain ABA-related protein family (CAR) involved in light and jasmonate signaling pathways |
作者: | Wei-Ting Liao 廖薇婷 |
指導教授: | 謝旭亮(Hsu-Liang Hsieh) |
關鍵字: | FUL,CAR,離層酸,茉莉酸,光, FUL,CAR,abscisic acid,jasmonate,light, |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 了解光及賀爾蒙所調控的果實發育之中的分子機制,對於提高作物產量是非常重要的。為了得到更多關於阿拉伯芥和番茄中FRUITFULL (FUL)參與在光及賀爾蒙調控果實發育的資訊,本研究以酵母菌雙雜合互補去氧核糖核酸基因庫(yeast two-hybrid cDNA library)釣取與FUL有交互作用的蛋白質,找到一C2-domain ABA-related protein family (CAR)成員。阿拉伯芥中CAR蛋白質家族包含10個成員CAR1~CAR10,參與調控PYR/PYL離層酸受體和細胞膜需要鈣離子的暫時性結合。在先前的研究中,CAR1、CAR4、CAR5和CAR9的car三重突變體在幼苗發育及根延長分析中對離層酸不敏感。CAR1和CAR4的晶體結構已經被解出,這代表可以更加清楚需要鈣離子的膜結合C2區域(C2 domain)的特有標誌,與PYR/PYL受體有交互作用的區域,以及他們到磷脂囊泡的吸收。然而,CAR的其他功能仍然是未知的。在不同光照與賀爾蒙處理的基因表現和種子發芽實驗中,我發現car突變體有不同的外表型,而且CAR可能在光及茉莉酸訊息傳遞途徑中扮演了不同的角色。光及茉莉酸訊息傳遞途徑在植物中調控不同的生理反應過程,包含發芽、抗病、逆境耐受、開花,以及果實成熟。CAR5可能參與果實成熟;car1和car9突變株在藍光下較敏感,而car1和car5則是對甲基化茉莉酸更加敏感。而且CAR1和CAR9會被甲基化茉莉酸(methyl jasmonate, MeJA)正調控。在酵母菌雙雜合和雙分子螢光互補(bimolecular fluorescence complementation, BiFC)實驗中,CAR4、5、9及FUL會分別和FIN219進行交互作用,CAR4會和CAR9有交互作用。總結來說,CAR家族不只能調控離層酸訊息傳遞路徑,也能參與在光及茉莉酸訊息傳遞途徑之中。 Understanding the molecular mechanisms underlying light- and hormone-mediated fruit development is critical in improving crop yields. To get more information about FRUITFULL (FUL) protein which is involved in the regulation of light- and hormone-mediated fruit development in both Arabidopsis and tomato, I used a yeast two-hybrid cDNA library to screen FUL protein interacting partners, and obtained a member of the C2-domain ABA-related protein family (CAR). The CAR protein family contain CAR1 to CAR10, they can mediate transient calcium-dependent interactions of PYR/PYL ABA receptors with membranes in Arabidopsis thaliana. In previous study, a car triple mutant among CAR1, CAR4, CAR5 and CAR9 was abscisic acid (ABA) insensitive in seedling establishment and root growth assays. The crystal structure of CAR1 and CAR4 was solved, which shows clearer specific signatures in calcium-dependent lipid binding C2 domain, interacting domain with PYR/PYL receptors and their recruitment to phospholipid vesicles (Diaz, M. et al., 2016). However, the other functions of CAR are still unknown. In gene expression and seed germination experiments under different hormone and light treatments, I found that car mutants showed different phenotypes and CARs may play different roles in light and Jasmonate signal pathways. Both light and Jasmonate signaling pathways regulate many diverse processes in plants, including germination, defense, stress tolerance, flowering and fruit ripening. CAR5 may join fruit ripening, car1 and car9 are more sensitive to blue light, car1 and car5 show more sensitivity to MeJA. In addition, CAR1 and CAR9 may be up-regulated by methyl jasmonate (MeJA). In yeast two-hybrid and Bimolecular Fluorescence Complementation (BiFC) data, CAR4, 5, 9 and FUL may interact with FIN219. CAR4 may physically interact with CAR9. To sum up, CARs not only can mediate ABA signaling, but also integrate light and Jasmonate signaling pathways. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58472 |
DOI: | 10.6342/NTU202001472 |
全文授權: | 有償授權 |
顯示於系所單位: | 植物科學研究所 |
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