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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 徐善慧(Shan-hui Hsu) | |
dc.contributor.author | Yan-Ping Chen | en |
dc.contributor.author | 陳彥平 | zh_TW |
dc.date.accessioned | 2021-06-16T08:13:18Z | - |
dc.date.available | 2019-03-18 | |
dc.date.copyright | 2014-03-18 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-02-14 | |
dc.identifier.citation | [1] Kruis FE, Fissan H, Peled A. Synthesis of nanoparticles in the gas phase for electronic, optical and magnetic applications – a review. J Aerosol Sci 1998;29:511-35.
[2] Wang LS, Chuang MC, Ho JA. Nanotheranostics – a review of recent publications. Int J Nanomedicine 2012;7:4679-95. [3] Pridgen EM, Langer R, Farokhzad OC. Biodegradable, polymeric nanoparticle delivery systems for cancer therapy. Nanomedicine 2007;2:669-80. [4] Ahmed N, Fessi H, Elaissari A. Theranostic applications of nanoparticles in cancer. Drug Discov Today 2012;17:928-34. [5] Lee JH, Cheon JW. Synergistically integrated nanoparticles as multimodal probes for nanobiotechnology. Acc Chem Res 2008;41:1630-40. [6] Klostranec JM, Chan WCW. Quantum dots in biological and biomedical research: recent progress and present challenges. Adv Mater 2006;18:1953-64. [7] Cho EC, Glaus C, Chen J, Welch MJ, Xia Y. Inorganic nanoparticle-based contrast agents for molecular imaging. Trends Mol Med 2010;16:561-73. [8] Soenen SJ, Pilar RG, Montenegro JM, Parak WJ, Smedt SCD , Braeckmans K. Cellular toxicity of inorganic nanoparticles: common aspects and guidelines for improved nanotoxicity evaluation. Nano Today 2011;6:446-65. [9] Gupta AK, Gupta M. Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 2005;26:3995-4021. [10] Hsu SH, Ho TT, Tseng TC. Nanoparticle uptake and gene transfer efficiency for MSCs on chitosan and chitosan-hyaluronan substrates. Biomaterials 2012;33:3639-50. [11] Li KG, Chen JT, Bai SS, Wen X, Song SY, Yu Q, Li J, Wang YQ. Intracellular oxidative stress and cadmium ions release induce cytotoxicity, of unmodified cadmium sulfide quantum dots. Toxicol In Vitro 2009; 23:1007-13. [12] Nguyen TK, Luke AW. Functionalisation of nanoparticles for biomedical applications. Nano Today 2010;5:213-30. [13] Zhu M, Nie G, Meng H, Xia T, Nel A, Zhao Y. Physicochemical properties determine nanomaterial cellular uptake, transport, and fate. Acc Chem Res 2013;46:622-31. [14] Wu W, He Q, Jiang C. Magnetic iron oxide nanoparticles: synthesis and surface functionalization strategies. Nanoscale Res Lett 2008;3:397-415. [15] Sun J, Zhou S, Hou P, Yang Y, Weng J, Li X, Li M. Synthesis and characterization of biocompatible Fe3O4 nanoparticles. J Biomed Mater Res A 2007;2:333-41. [16] Grubbs RB. Roles of polymer ligands in nanoparticle stabilization. Polym Rev 2007;47:197-215. [17] Jeon HJ, Jeong YI, Jang MK, Park YH, Nah JW. Effect of solvent on the preparation of surfactant-free poly(DL-lactide-co-glycolide) nanoparticles and norfloxacin release characteristics. Int J Pharm 2000;207:99-108. [18] Gupta AK, Wells S. Surface-modified superparamagnetic nanoparticles for drug delivery: preparation, characterization, and cytotoxicity studies. IEEE T Nanobiosci 2004;3:66-73. [19] Berry CC, Wells S, Charles S, Curtis ASG. Dextran and albumin derivatised iron oxide nanoparticles: influence on fibroblasts in vitro. Biomaterials 2003;24:4551-7. [20] Li W, Xiao L, Qin CQ. The characterization and thermal investigation of chitosan-Fe3O4 nanoparticles synthesized via a novel one-step modifying process. j macromol sci A 2010;48:57-64. [21] Wang Y, Li B, Xu F, Jia D, Feng Y, Zhou Y. In vitro cell uptake of biocompatible magnetite/chitosan nanoparticles with high magnetization: a single-step synthesis approach for In-situ-modified magnetite by amino groups of chitosan. J Biomater Sci Polym Ed 2013;23:843-60. [22] Olsen D, Yang C, Bodo M, Chang R, Leigh S, Baez J, Carmichael D, Perala M, Hamalainen ER, Jarvinen M, Polarek J. Recombinant collagen and gelatin for drug delivery. Adv Drug Deliv Rev 2003;55:1547-67. [23] KievitFM, Zhang MQ.Surface engineering of iron oxide nanoparticles for targeted cancer therapy. Acc Chem Res 2011;44:853-62. [24] Soppimath KS, Aminabhavi TM, Kulkarni AR, Rudzinski WE. Biodegradable polymeric nanoparticles as drug delivery devices. J Controlled Release 2001;70:1-20. [25] Panyam J, Labhasetwar V. Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Deliv Rev 2012;64:61-71. [26] Gref R, Minamitake Y, Peracchia MT, Trubetskoy V, Torchilin V, Langer R. Biodegradable long-circulating polymeric nanospheres. Science 1994;263:1600-3. [27] Bae SJ, Park JA, Lee JJ, Lee GH, Kim TJ, Yoo DS, Chang Y. Ultrasmall iron oxide nanoparticles: synthesis, physicochemical, and magnetic properties. Curr Appl Phys 2009;9:19-21. [28] Ahmed N, Michelin-Jamois M, Fessi H, Elaissari A. Modified double emulsion process as a new route to prepare submicron biodegradable magnetic/polycaprolactone particles for in vivo theranostics. Soft Matter 2012;8:2554-64. [29] Rutnakornpituk M, Meerod S, Boontha B, Wichai U. Magnetic core-bilayer shell nanoparticle: A novel vehicle for entrapment of poorly water-soluble drugs. Polymer 2009;50:3508-15. [30] Eriksson T, Borjesson J, Tjerneld F. Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzyme Microb Technol 2002;31:353-64. [31] Kim BK, Yang JS, Yoo SM, Lee JS. Waterborne polyurethanes containing ionic groups in soft segments. Colloid Polym Sci 2003;281:461-8. [32] Jaudouin O, Robin JJ, Lopez-Cuesta JM, Perrin D, Imbert C. Ionomer-based polyurethanes: a comparative study of properties and applications. Polym Int 2012;61:495-510. [33] Noble KL. Waterborne polyurethanes. Prog Org Coat 1997;32:131-6. [34] Lee SY, Lee JS, Kim BK. Preparaton and properties of water-borne polyurethanes. Polymer Internationalt 1997;42:67-76. [35] Rahman MM, Kim HD. Synthesis and characterization of waterborne polyurethane adhesives containing different amount of ionic group (I). J Appl Polym Sci. 2006;102:5684-91. [36] Gunatillake P, Mayadunne R, Adhikari R. Recent developments in biodegradable polymers. Biotechnol Annu Rev 2006;12:301-47. [37] Santerre JP, Woodhouse K, Laroche G, Labow RS. Understanding the biodegradation of polyurethanes: from classical implants to tissue engineering materials. Biomaterials 2005;26:7457-70. [38] Younes HM, Bravo-Grimaldo E, Amsden BG. Synthesis, characterization and in-vitro degradation of a biodegradable elastomer. Biomaterials. 2004;25:5261-9. [39] Guelcher SA. Biodegradable polyurethanes: synthesis and applications in regenerative medicine.Tissue Eng Part B Rev 2008;14:3-17. [40] Loh X. J, Tan KK, Li X, Li J. The in-vitro hydrolysis of poly (ester urethane)s consisting of poly[(R)-3-hydroxybutyrate] and poly (ethylene glycol). Biomaterials 2005;27:1841-50. [41] Ding M, Li J, Tan H, Fu Q. Self-assembly of biodegradable polyurethanes for controlled delivery applications. Soft Matter 2012;8:5414-28. [42] Zhu QS, Wang Y, Zhou M, Mao C, Huang XH, Bao JC, Shen J. Preparation of anionic polyurethane nanoparticles and blood compatible behaviors. J Nanosci Nanotechno 2012;12:4051-6. [43] Khosroushahi AY, Naderi-Manesh H, Yeganeh H, Barar J, Omidi Y. Novel water-soluble polyurethane nanomicelles for cancer chemotherapy: physicochemical characterization and cellular activities. J Nanobiotechnology 2012;10:2. [44] Zhang J, Wu M, Yang J, Wu Q, Jin Z. Anionic poly (lactic acid)-polyurethane micelles as potential biodegradable drug delivery carriers. Colloids Surf A Physicochem Eng Asp 2009;337:200-4. [45] Gong CY, Wei XW, Wang XH, Wang YJ, Guo G, Mao YQ, Luo F, Qian ZY. Biodegradable self-assembled PEG-PCL-PEG micelles for hydrophobic honokiol delivery: I. preparation and characterization. Nanotechnology 2010;21:215103. [46] Gong CY, Wang YJ, Wang XH, Wei XW, Wu QJ, Wang BL, Dong PW, Chen LJ, Luo F, Qian ZY. Biodegradable self-assembled PEG-PCL-PEG micelles for hydrophobic drug delivery, part 2: in vitro and in vivo toxicity evaluation. J Nanopart Res 2011;13:721-31. [47] Zhang SB, Lv HT, Zhang H, Wang B, Xu YM. Waterborne polyurethanes: spectroscopy and stability of emulsions. J Appl Polym Sci 2005;101:597-602. [48] Das B, Mandal M, Upadhyay A, Chattopadhyay P, Karak N. Bio-based hyperbranched polyurethane/Fe3O4 nanocomposites:smart antibacterial biomaterials for biomedical devices and implants. Biomed Mater 2013;8:035003. [49] Srichatrapimuk VW, Cooper SL. Infrared thermal analysis of polyurethane block polymers. J Macromol Sci Phys 1978;15:267-311. [50] Hung HS, Chu MY, Lin CH, Wu CC, Hsu SH. Mediation of the migration of endothelial cells and fibroblasts on polyurethane nanocomposites by the activation of integrin-focal adhesion kinase signaling. J Biomed Mater Res A 2012;100A:26-37. [51] Guo ZH, Lei K, Li YT, Ng HW, Prikhodko S, Hahn HT. Fabrication and characterization of iron oxide nanoparticles reinforced vinyl-ester resin nanocomposites. Compos Sci Technol 2008;68:1513-20. [52] Giardiello M, McDonald TO, Martin P, Owen A, Rannard SP. Facile synthesis of complex multi-component organic and organic-magnetic inorganic nanocomposite particles. J Mater Chem 2012;22:24744-52. [53] Chantrell RW, Popplewell J, Charles SW. Measurements of particle-size distribution parameters in ferrofluids. IEEE T Magn 1978;14:975-7. [54] Glover AL, Bennett JB, Pritchett JS, Nikles SM, Nikles DE, Nikles JA, Brazel CS. Magnetic heating of iron oxide nanoparticles and magnetic micelles for cancer therapy. IEEE T Magn 2013;49:231-5. [55] Liu TY, Hu SH, Liu DM, Chen SY, Chen IW. Biomedical nanoparticle carriers with combined thermal and magnetic responses. Nano Today 2009;4:52-65. [56] Li TJ, Huang CC, Ruan PW, Chuang KY, Huang KJ, Shieh DB, Yeh CS. In vivo anti-cancer efficacy of magnetite nanocrystal-based system using locoregional hyperthermia combined with 5-fluorouracil chemotherapy. Biomaterials 2013;34:7873-83. [57] Giljohann DA, Seferos DS, Patel PC, Millstone JE, Rosi NL, Mirkin CA. Oligonucleotide loading determines cellular uptake of DNA-modified gold nanoparticles. Nano Lett 2007;7:3818-21. [58] Liao MH, Chen DH. Fast and efficient adsorption/desorption of protein by a novel magnetic nano-adsorbent. Biotechnol Lett 2002;24:1913-17. [59] Tremblay P, Lesage J, Ostiguy C, Tra HV. Investigation of the competitive rate of derivatization of several secondary amines with phenylisocyanate (PHI),hexamethylene-1,6-diisocyanate (HDI), 4,4A-methylenebis(phenyl isocyanate) (MDI) and toluene diisocyanate (TDI) in liquid medium. Analyst 2003;128:142-9. [60] Xiong XY, Tam KC, Gan LH. Release kinetics of hydrophobic and hydrophilic model drugs from pluronic F127/poly(lactic acid) nanoparticles. J Control Release 2005;103:73-82. [61] Doring O, Luthje S, Bottger M. Modification of the activity of the plasma membrane redox system of Zea mays L. roots by vitamin K3 and dicumarol. J exp bot 1992;43:175-81. [62] Lin TC, Chen JH, Chen YH, Teng TM, Su CH, Hsu SH.Biodegradable micelles from a hyaluronan-poly-(3-caprolactone) graft copolymer as nanocarriers for fibroblast growth factor 1. J Mater Chem B 2013;1:5977-87. [63] Wang Y, Wu G, Li X, Wang Y, Gao H, Ma J. Synthesis, characterization and controlled drug release from temperature-responsive poly(ether-urethane) particles based on PEG-diisocyanates and aliphatic diols. J Biomater Sci Polym Ed 2013;24:1676-91. [64] Fresta M, Puglisi G, Giammona G, Cavallaro G, Micali N, Furneri PM. Pefloxacine mesilate- and ofloxacin-loaded polyethylcyanoacrylate nanoparticles: characterization of the colloidal drug carrier formulation. J Pharm Sci 1995;84:895-902. [65] Osada S, Tomita H, Tanaka Y, Tokuyama Y, Tanaka H, Sakashita F, Takahashi T. The utility of vitamin K3 (menadione) against pancreatic cancer. Anticancer Res 2008;28:45-50. [66] Tareen B, Summers JL, Jamison JM, Neal DR, McGuire K, Gerson L, Diokno A. A 12 week, open label, phase I/IIa study using ApatoneR for the treatment of prostate cancer patients who have failed standard therapy. Int J Med Sci 2008;5:62-7. [67] Jamison JM, Gilloteaux J, Taper HS, Summers JL. Evaluation of the In Vitro and In vivo antitumor activities of vitamin C and K-3 combinations against human prostate cancer. J Nutr 2001;131:158-60. [68] Rapoport N. Physical stimuli-responsive polymeric micelles for anti-cancer drug delivery. Prog Polym Sci 2007;32:962-90. [69] Wiradharma N, Zhang Y, Venkataraman S, Hedrick JL, Yang YY. Self-assembled polymer nanostructures for delivery of anticancer therapeutics. Nano Today 2009;4:302-17. [70] Mao C, Jiang LC, Luo WP, Liu HK, Bao JC, Huang XH, Shen J. Novel blood-compatible polyurethane ionomer nanoparticles. Macromolecules 2009;42:9366-8. [71] Ding MM, Song NJ, He XL, Li JH, Zhou LJ, Tan H, Fu Q, Gu Q. Toward the next-generation nanomedicines: design of multifunctional multiblock polyurethanes for effective cancer treatment. ACS Nano 2013;7:1918-28. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58384 | - |
dc.description.abstract | 傳統超順磁氧化鐵奈米粒(SPIO NPs)具備MRI顯影和過高熱治療的生醫應用,又治療癌症之抗癌藥物為疏水性。本研究使用水性生物可降解聚胺酯的水分散並行自組裝成為奈米粒(PU NPs)之行為,將超順磁氧化鐵奈米粒子或疏水性模擬藥物分別以原位(in situ)混合方式包覆成為超順磁氧化鐵-水性生物可降解聚胺酯奈米粒(SPIO-PU NPs)和藥物-水性生物可降解聚胺酯奈米粒(drug-PU NPs)。先以動態光散射評估包覆結果皆為奈米粒型式。使用穿透式電子顯微鏡、傅立葉紅外線光譜和熱重分析證明SPIO-PU NPs表面為PU高分子,以超導量子磁干涉儀與交流磁場裝置分析其保持原有SPIO超順磁性與磁場加熱功能,同時具備標定癌細胞能力。透過離心超濾法可分析此PU NPs對疏水性藥物具備高包覆效率與藥物釋放,也發現隨著藥物結構中的官能基若與PU預聚合物反應,會產生化學性或物理性之包覆結果並影響藥物釋放能力,而物理性包覆的drug-PU NPs具有良好的藥物釋放行為,進一步得知可經由溫度提高加速藥物釋放。PU NPs無顯著細胞毒性,又藥物之螢光賦予PU NPs具有螢光的能力,依此證明癌細胞亦可攝取PU NPs予以標定。因此本研究PU NPs具有能搭配診斷與雙重治療癌症的潛力。 | zh_TW |
dc.description.abstract | Superparamagnetic iron oxide nanoparticles (SPIO NPs) are widely used in magnetic resonance imaging and magnetic hyperthermia. In this study, we used the self-assembly behavior of biodegradable polyurethane nanoparticles (PU NPs) in water to encapsulate SPIO NPs (SPIO-PU NPs) or hydrophobic model drugs (drug-PU NPs) by an in-situ method. PU NPs and SPIO-PU NPs were characterized by the dynamic light scattering (DLS), transmission electron microscopy (TEM), infrared spectroscopy (IR), and thermogravimetric analysis (TGA). The superparamagnetic property and magnetic heating ability of SPIO-PU NPs were assessed. PU NPs had no significant cytotoxicity and could be taken up by cells. SPIO-PU NPs were highly efficient in labeling cancer cells with cellular uptake of ~16 pg per cell in average. Hydrophobic drugs were entrapped in PU NPs effectively and showed a sustained release profile. Upon heating, the release of drug was accelerated. This proof-of-concept study demonstrated a novel way to encapsulate SPIO and hydrophobic drug in PU NPs with smart designs for potential applications in cancer diagnostics, hyperthermia, and chemotherapy. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T08:13:18Z (GMT). No. of bitstreams: 1 ntu-103-R01549014-1.pdf: 6895527 bytes, checksum: 829c4e5110a78fbd53ee75ac3dc3653b (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 口試委員會審定書 i
致謝 ii 摘要 iii Abstract iv 圖目錄 viii 表目錄 xi 第一章 文獻回顧 1 1.1. 無機奈米粒子與生醫應用 1 1.2. 磁性與光學奈米粒子特性用於生醫應用 2 1.3. 奈米粒子毒性與應用 3 1.4. 奈米粒子表面修飾與改質 4 1.4.1. 氧化鐵(SPIO)奈米粒子表面改質 4 1.5. 奈米粒子表面高分子塗層 5 1.5.1氧化鐵(SPIO)奈米粒子表面高分子塗層 5 1.6. 聚胺酯 6 1.6.1. 油性/水性/離子體聚胺酯 6 1.6.2. 生物可降解聚胺酯 7 1.6.3. 水性聚胺酯奈米粒子之生醫應用與安全性 8 1.7. 研究動機與目的 9 第二章 研究方法 10 2.1. 研究架構 10 2.2. 水性生物可降解聚胺酯包覆疏水奈米粒子與藥物之製備 12 2.2.1. 水性生物可降解聚胺酯奈米粒子(Water-based biodegradable polyurethane nanoparticles, PU NPs)合成 12 2.2.2. 氧化鐵-生物可降解聚胺酯奈米粒子(SPIO-PU NPs)製備 14 2.2.3. 疏水性藥物-生物可降解聚胺酯奈米粒子(drug-PU NPs)製備 16 2.3. 氧化鐵-生物可降解聚胺酯奈米粒子物化特性分析 18 2.3.1. 粒徑與表面電位分析 18 2.3.2. 穿透式電子顯微鏡分析 18 2.3.3. 全反射傅立葉紅外線光譜分析 18 2.3.4. 熱重分析儀分析 19 2.3.5. 磁化性質分析 20 2.3.6. 高週波磁場加熱測試 20 2.3.7. SPIO-PU NPs細胞活性與攝取實驗 21 2.4. 疏水性藥物-生物可降解聚胺酯奈米粒子分析 23 2.4.1. 粒徑與界面電位分析 23 2.4.2. 藥物溶液檢量線製作與濃縮離心管測試分析 23 2.4.3. 藥物釋放實驗 24 2.4.4. PU NPs細胞活性與攝取 25 第三章 實驗結果 27 3.1. 水性生物可降解聚胺酯合成並包覆氧化鐵奈米粒子之物化分析與細胞實驗 27 3.1.1. 水性生物可降解聚胺酯奈米粒子(PU NPs)合成 27 3.1.2. 氧化鐵-生物可降解聚胺酯奈米粒子(SPIO-PU NPs)製備 27 3.1.3. 粒徑與表面電位分析 28 3.1.4. 穿透式電子顯微鏡分析 28 3.1.5. 傅立葉紅外線光譜分析 28 3.1.6. 熱性質分析 29 3.1.7. 磁化性質分析 29 3.1.8. 高週波磁場加熱測試 29 3.1.9. SPIO-PU NPs之細胞活性與攝取實驗 30 3.2. 藥物-生物可降解聚胺酯奈米粒子(drug-PU NPs)之分析與藥物釋放 31 3.2.1. 藥物檢量線與濃縮離心管測試 31 3.2.2. 藥物-生物可降解聚胺酯奈米粒子之分析 31 3.2.3. 藥物-生物可降解聚胺酯奈米粒子藥物釋放 32 3.3. 生物可降解聚胺酯奈米粒子之細胞活性與細胞攝取實驗 33 3.3.1. PU NPs與MAMA-PU NPs之細胞活性 33 3.3.2.細胞攝取MAMA-PU NPs實驗 33 第四章 討論 34 4.1. 水性生物可降解聚胺酯合成並包覆氧化鐵奈米粒子之物化分析與細胞實驗 34 4.1.1. 水性生物可降解聚胺酯奈米粒子(PU NPs)合成 34 4.1.2. 氧化鐵-生物可降解聚胺酯奈米粒子(SPIO-PU NPs)製備 34 4.1.3. 粒徑與表面電位分析 35 4.1.4. 穿透式電子顯微鏡分析 35 4.1.5. 傅立葉紅外線光譜分析 35 4.1.6. 熱性質分析 36 4.1.7. 磁化性質分析 37 4.1.8. 高週波磁場加熱測試 37 4.1.9. SPIO-PU NPs之細胞活性與攝取實驗 38 4.2. 藥物-生物可降解聚胺酯奈米粒子(drug-PU NPs)之分析與藥物釋放 39 4.2.1. 藥物檢量線與濃縮離心管測試 39 4.2.2. 藥物-生物可降解聚胺酯奈米粒子之分析 39 4.2.3. 藥物-生物可降解聚胺酯奈米粒子藥物釋放 40 4.3. 生物可降解聚胺酯奈米粒子之細胞活性與細胞攝取實驗 42 4.3.1. PU NPs與MAMA-PU NPs之細胞活性 42 4.3.2. 細胞攝取MAMA-PU NPs實驗 42 第五章 結論 44 參考文獻 65 | |
dc.language.iso | zh-TW | |
dc.title | 新穎水性可降解聚胺酯奈米粒包覆超順磁氧化鐵與疏水性藥物之製備與分析 | zh_TW |
dc.title | Preparation and characterization of novel water-based biodegradable polyurethane nanoparticles encapsulating superparamagnetic iron oxide and hydrophobic drug | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉定宇(Ting-Yu Liu),林政鞍(Cheng-An J. Lin),孫一明(Yi-Ming Sun),胡孝光(Shiaw-Guang Hu) | |
dc.subject.keyword | 磁奈米粒子,超順磁氧化鐵,疏水性藥物,生物可降解聚胺酯,自組裝,細胞標定, | zh_TW |
dc.subject.keyword | magnetic nanoparticles,superparamagnetic iron oxide (SPIO NPs),hydrophobic drug,biodegradable polyurethane,self-assembly,cell labeling, | en |
dc.relation.page | 72 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-02-14 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
顯示於系所單位: | 高分子科學與工程學研究所 |
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