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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58052完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 朱士維(Shi-Wei Chu) | |
| dc.contributor.author | Kuo-Jen Hsu | en |
| dc.contributor.author | 徐國仁 | zh_TW |
| dc.date.accessioned | 2021-06-16T08:05:09Z | - |
| dc.date.available | 2017-08-11 | |
| dc.date.copyright | 2014-08-11 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-06-26 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58052 | - |
| dc.description.abstract | 中樞神經系統的神經生理活動一直都與腦如何運作有很大的關係。眾多神經退化性疾病譬如: 阿茲海默氏症、帕金森氏症、杭丁頓舞蹈症等等，都與神經系統的運作息息相關，因此研究活體神經功能在今日極為重要。就我們所知，神經網路具有高度複雜的三維空間結構，同時神經活動發生在數毫秒內，而神經細胞大小約數微米，因此研究神經活動的方法必須具有高的時間與空間解析度且同時具有解析三維空間的能力。
傳統電生理學雖可提供非常好的時間解析度以及相當高的訊雜比，然而，在空間上要在兩個相鄰的神經細胞插上電極直至目前為止仍然是相當困難的；不僅如此，其侵入式的做法是否會影響神經細胞本身的生理狀態仍有待商榷。而功能性磁共振成像雖提供了非侵入式的做法並大大提高了穿透深度到足以觀察人腦的神經活動，但其時間與空間的解析度卻不足以分辨單一神經細胞活動。 相較之下，光學成像技術可以在活體內具有單一神經細胞的空間解析度，並且可同時觀察多個神經細胞活動，即是一項值得神經研究嘗試使用的方法。傳統顯微技術如共軛焦、多光子顯微術等雖然具有光學切片能力而能夠提供良好的影像對比，但應用在神經活動的研究上卻無法同時觀察到軸向上分離的神經活動，這項缺失進而促使了高速三維光學成像技術的發展。到目前為止的進展譬如聲光偏轉器、三維空間雷射掃描等技術利用空間局部取樣的方法增加取樣速度已被證實可以同時監控軸向分離的神經活動，然而空間局部取樣使用的前提是依賴在樣本具有非常好的空間穩定性，這卻是在活體研究上難以達成的。因此這篇論文採用聲光可調梯度折射率透鏡結合光學切片雷射掃描顯微鏡軸向延展焦點，進而實現相對傳統共軛焦、多光子顯微術更厚的光學切片，組成影像研究空間中軸向分開的神經活動，利用影像觀察神經活動將不受限於樣本的空間穩定性且可提供局部資訊以利科學家判定細胞種類及功能，這就是全焦距功能性成像系統的概念。 聲光可調梯度折射率透鏡已有文獻證實不論是結合雷射掃描或是明視野顯微鏡都可以提供相對傳統共軛焦、多光子顯微術更大的景深，而不同的景深可藉由調控聲波震幅以及頻率獲得。雖然這項技術已被應用在觀察果蠅幼蟲的生長，但卻尚未有文獻將這項技術優化使得焦點延展的長度符合理論預測，並且均勻化軸向的影像對比進一步將其應用在神經活動的研究。因此這篇論文中也將探討如何優化這項技術並應用其為實現全焦距功能性成像系統的研究方法。 本篇論文的研究對象是果蠅。短的生命週期、龐大的子代數量以及眾多的基因變異使牠成為一個良好的模式生物。果蠅的大腦由約十萬顆神經細胞構成，雖然相對於人腦的一百億顆，這是個微小的數目；但這樣的結構足以使果蠅具有簡單的行為模式以利科學家研究建立模型以簡化研究人腦的困難度。而在眾多感覺及行為中，嗅覺相對來說較為容易操控與理解，而果蠅的嗅覺傳導機制和人類很接近，因此這篇論文選擇果蠅的嗅覺神經系統為研究目標。利用全焦距功能性成像系統證實可同時觀察到在果蠅腦內軸向分離數十甚至上百微米不同區塊的嗅覺神經活動。這項結果不僅在技術上是一項重大突破，在生物研究上也是世界上第一個果蠅全腦功能性成像，這項具備高空間解析度且非侵入式的技術相信在未來能夠提供神經科學家一項強而有力的研究工具。 | zh_TW |
| dc.description.abstract | Neural network dynamics in central nervous system are the key to resolve a big question, how the brain functions, nowadays. Many neural diseases such as Alzheimer’s, Parkinson’s and Huntington’s, are directly linked to brain functions. As a result, in order to reveal the secrets behind these diseases, in vivo neurophysiology researches are important. Neural networks exhibits complex 3-dimensional (3D) structures and neural events have temporal and spatial scales on millisecond (ms) and micrometer ( | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T08:05:09Z (GMT). No. of bitstreams: 1 ntu-103-R01222011-1.pdf: 3985162 bytes, checksum: 13313e8a7f9f51b4bec21cc9ddfcb45f (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 口試委員會審定書 II
致謝 III 中文摘要 V ABSTRACT VII CONTENTS X Chapter 1. Introduction 1 Chapter 2. Optical Methods for Neural Study 4 2.1 Historical review 4 2.2 Obstacles of optical methods for neural study 5 2.3 Current 3D optical methods for neural study 5 2.3.1. Multifocal multiphoton microscopy (MMM) 6 2.3.2. 3D laser line scanning 7 2.3.3. Random access microscopy (RAM) 7 2.3.4. Others 9 2.4 Strategy-TAG lens 15 2.4.1. Introduction 15 2.4.2. Comparison with other research 18 Chapter 3. Sample 30 3.1 Drosophila Melanogaster (D. Melanogaster) 30 3.2 Olfactory system 30 3.3 Genetically encoded calcium indicators (GECIs) 33 Chapter 4. Experiments and Results 34 4.1 Temporal modulation of TAG Lens 34 4.1.1. Setup 34 4.1.2. Steps 36 4.1.3. Results 37 4.2 PSF measurement and test on fluorescence study 37 4.2.1. Setup 38 4.2.2. Steps 39 4.2.3. Results 40 4.3 All-in-focus imaging 46 4.3.1. Setup 47 4.3.2. Steps 48 4.3.3. Results 48 4.4 All-in-focus functional imaging 51 4.4.1. Setup 51 4.4.2. Steps 52 4.4.3. Results 53 Chapter 5. Discussion 58 5.1 PSF measurement and test on fluorescence study 58 5.2 All-in-focus functional imaging 59 Chapter 6. Conclusion and Prospect 63 Figure list 66 Table list 69 References 70 | |
| dc.language.iso | en | |
| dc.subject | 全焦距 | zh_TW |
| dc.subject | 果蠅 | zh_TW |
| dc.subject | 雷射掃描顯微鏡 | zh_TW |
| dc.subject | 變焦透鏡 | zh_TW |
| dc.subject | Drosophila | en |
| dc.subject | laser scanning microscopy | en |
| dc.subject | all-in-focus | en |
| dc.subject | variable lens | en |
| dc.title | 全焦距功能性成像系統應用於果蠅腦內活動之研究 | zh_TW |
| dc.title | All-in-focus Functional Imaging System for Drosophila Brain Activities Study | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林彥穎(Yen-Yin Lin),江安世(Ann-Shyn Chiang) | |
| dc.subject.keyword | 變焦透鏡,雷射掃描顯微鏡,全焦距,果蠅, | zh_TW |
| dc.subject.keyword | variable lens,laser scanning microscopy,all-in-focus,Drosophila, | en |
| dc.relation.page | 76 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-06-26 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 物理研究所 | zh_TW |
| 顯示於系所單位： | 物理學系 | |
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