請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58017
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 游佳欣(Jiashing Yu) | |
dc.contributor.author | I-Chun Chen | en |
dc.contributor.author | 陳宜駿 | zh_TW |
dc.date.accessioned | 2021-06-16T08:04:36Z | - |
dc.date.available | 2020-08-06 | |
dc.date.copyright | 2020-08-06 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-07-31 | |
dc.identifier.citation | 1. Murphy, C. M.; O'Brien, F. J.; Little, D. G.; Schindeler, A., Cell-scaffold interactions in the bone tissue engineering triad. Eur Cell Mater 2013, 26, 120-32. 2. Bhardwaj, N.; Chouhan, D.; Mandal, B. B., 14 - 3D functional scaffolds for skin tissue engineering. In Functional 3D Tissue Engineering Scaffolds, Deng, Y.; Kuiper, J., Eds. Woodhead Publishing: 2018; pp 345-365. 3. Wang, H. M.; Chou, Y. T.; Wen, Z. H.; Wang, C. Z.; Chen, C. H.; Ho, M. L., Novel biodegradable porous scaffold applied to skin regeneration. PLoS One 2013, 8 (6), e56330. 4. Ma, L.; Gao, C.; Mao, Z.; Zhou, J.; Shen, J.; Hu, X.; Han, C., Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials 2003, 24 (26), 4833-4841. 5. Tang, K. C.; Yang, K. C.; Lin, C. W.; Chen, Y. K.; Lu, T. Y.; Chen, H. Y.; Cheng, N. C.; Yu, J., Human Adipose-Derived Stem Cell Secreted Extracellular Matrix Incorporated into Electrospun Poly(Lactic-co-Glycolic Acid) Nanofibrous Dressing for Enhancing Wound Healing. Polymers (Basel) 2019, 11 (10). 6. Poursamar, S. A.; Hatami, J.; Lehner, A. N.; da Silva, C. L.; Ferreira, F. C.; Antunes, A. P. M., Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment. Materials Science and Engineering: C 2015, 48, 63-70. 7. Harris, L. D.; Kim, B. S.; Mooney, D. J., Open pore biodegradable matrices formed with gas foaming. J Biomed Mater Res 1998, 42 (3), 396-402. 8. Pezeshki-Modaress, M.; Rajabi-Zeleti, S.; Zandi, M.; Mirzadeh, H.; Sodeifi, N.; Nekookar, A.; Aghdami, N., Cell-loaded gelatin/chitosan scaffolds fabricated by salt-leaching/lyophilization for skin tissue engineering: in vitro and in vivo study. J Biomed Mater Res A 2014, 102 (11), 3908-17. 9. Wake, M. C.; Gupta, P. K.; Mikos, A. G., Fabrication of Pliable Biodegradable Polymer foams to Engineer Soft Tissues. Cell Transplantation 1996, 5 (4), 465-473. 10. Schmedlen, R. H.; Masters, K. S.; West, J. L., Photocrosslinkable polyvinyl alcohol hydrogels that can be modified with cell adhesion peptides for use in tissue engineering. Biomaterials 2002, 23 (22), 4325-4332. 11. Wu, Z.; Su, X.; Xu, Y.; Kong, B.; Sun, W.; Mi, S., Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation. Sci Rep 2016, 6, 24474. 12. Pereira, R. F.; Barrias, C. C.; Bártolo, P. J.; Granja, P. L., Cell-instructive pectin hydrogels crosslinked via thiol-norbornene photo-click chemistry for skin tissue engineering. Acta Biomaterialia 2018, 66, 282-293. 13. Zhao, X.; Lang, Q.; Yildirimer, L.; Lin, Z. Y.; Cui, W.; Annabi, N.; Ng, K. W.; Dokmeci, M. R.; Ghaemmaghami, A. M.; Khademhosseini, A., Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering. Advanced healthcare materials 2016, 5 (1), 108-18. 14. Full-Thickness Skin Wound Healing Using Human Placenta-Derived Extracellular Matrix Containing Bioactive Molecules. Tissue Eng Pt A 2013, 19 (3-4), 329-339. 15. Hoshiba, T.; Lu, H.; Kawazoe, N.; Chen, G., Decellularized matrices for tissue engineering. Expert Opin Biol Ther 2010, 10 (12), 1717-28. 16. Gunatillake, P. A.; Adhikari, R., Biodegradable synthetic polymers for tissue engineering. Eur Cell Mater 2003, 5, 1-16; discussion 16. 17. Martello, F.; Tocchio, A.; Tamplenizza, M.; Gerges, I.; Pistis, V.; Recenti, R.; Bortolin, M.; Del Fabbro, M.; Argentiere, S.; Milani, P.; Lenardi, C., Poly(amido-amine)-based hydrogels with tailored mechanical properties and degradation rates for tissue engineering. Acta Biomaterialia 2014, 10 (3), 1206-1215. 18. Armentano, I.; Bitinis, N.; Fortunati, E.; Mattioli, S.; Rescignano, N.; Verdejo, R.; Lopez-Manchado, M. A.; Kenny, J. M., Multifunctional nanostructured PLA materials for packaging and tissue engineering. Progress in Polymer Science 2013, 38 (10), 1720-1747. 19. Gentile, P.; Chiono, V.; Carmagnola, I.; Hatton, P. V., An overview of poly(lactic-co-glycolic) acid (PLGA)-based biomaterials for bone tissue engineering. Int J Mol Sci 2014, 15 (3), 3640-59. 20. Kweon, H.; Yoo, M. K.; Park, I. K.; Kim, T. H.; Lee, H. C.; Lee, H.-S.; Oh, J.-S.; Akaike, T.; Cho, C.-S., A novel degradable polycaprolactone networks for tissue engineering. Biomaterials 2003, 24 (5), 801-808. 21. VandeVord, P. J.; Matthew, H. W. T.; DeSilva, S. P.; Mayton, L.; Wu, B.; Wooley, P. H., Evaluation of the biocompatibility of a chitosan scaffold in mice. Journal of Biomedical Materials Research 2002, 59 (3), 585-590. 22. Croisier, F.; Jérôme, C., Chitosan-based biomaterials for tissue engineering. European Polymer Journal 2013, 49 (4), 780-792. 23. Thein-Han, W. W.; Misra, R. D. K., Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering. Acta Biomaterialia 2009, 5 (4), 1182-1197. 24. Rowley, J. A.; Madlambayan, G.; Mooney, D. J., Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 1999, 20 (1), 45-53. 25. Supramaniam, J.; Adnan, R.; Mohd Kaus, N. H.; Bushra, R., Magnetic nanocellulose alginate hydrogel beads as potential drug delivery system. International Journal of Biological Macromolecules 2018, 118, 640-648. 26. Ashton, R. S.; Banerjee, A.; Punyani, S.; Schaffer, D. V.; Kane, R. S., Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture. Biomaterials 2007, 28 (36), 5518-5525. 27. Tan, W. H.; Takeuchi, S., Monodisperse alginate hydrogel microbeads for cell encapsulation. Advanced Materials 2007, 19 (18), 2696-+. 28. Necas, J.; Bartošíková, L.; Brauner, P.; Kolár, J., Hyaluronic acid (hyaluronan): a review. Veterinarni Medicina 2008, 53, 397-411. 29. Kim, I. L.; Mauck, R. L.; Burdick, J. A., Hydrogel design for cartilage tissue engineering: A case study with hyaluronic acid. Biomaterials 2011, 32 (34), 8771-8782. 30. Monteiro, I. P.; Shukla, A.; Marques, A. P.; Reis, R. L.; Hammond, P. T., Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering. J Biomed Mater Res A 2015, 103 (1), 330-40. 31. Wang, T.-W.; Spector, M., Development of hyaluronic acid-based scaffolds for brain tissue engineering. Acta Biomaterialia 2009, 5 (7), 2371-2384. 32. Tan, H.; Ramirez, C. M.; Miljkovic, N.; Li, H.; Rubin, J. P.; Marra, K. G., Thermosensitive injectable hyaluronic acid hydrogel for adipose tissue engineering. Biomaterials 2009, 30 (36), 6844-6853. 33. Tiwari, S.; Patil, R.; Bahadur, P., Polysaccharide Based Scaffolds for Soft Tissue Engineering Applications. Polymers 2019, 11 (1), 1. 34. Chaudhari, A. A.; Vig, K.; Baganizi, D. R.; Sahu, R.; Dixit, S.; Dennis, V.; Singh, S. R.; Pillai, S. R., Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review. Int J Mol Sci 2016, 17 (12). 35. Shoulders, M. D.; Raines, R. T., Collagen Structure and Stability. Annual Review of Biochemistry 2009, 78 (1), 929-958. 36. Nimni, M. E., Collagen: Structure, function, and metabolism in normal and fibrotic tissues. Seminars in Arthritis and Rheumatism 1983, 13 (1), 1-86. 37. Glowacki, J.; Mizuno, S., Collagen scaffolds for tissue engineering. Biopolymers 2008, 89 (5), 338-344. 38. Spoerl, E.; Wollensak, G.; Dittert, D. D.; Seiler, T., Thermomechanical Behavior of Collagen-Cross-Linked Porcine Cornea. Ophthalmologica 2004, 218 (2), 136-140. 39. Designed Three-Dimensional Collagen Scaffolds for Skin Tissue Regeneration. Tissue Engineering Part C: Methods 2010, 16 (5), 813-820. 40. Lawrence, B. D.; Marchant, J. K.; Pindrus, M. A.; Omenetto, F. G.; Kaplan, D. L., Silk film biomaterials for cornea tissue engineering. Biomaterials 2009, 30 (7), 1299-1308. 41. Altman, G. H.; Diaz, F.; Jakuba, C.; Calabro, T.; Horan, R. L.; Chen, J.; Lu, H.; Richmond, J.; Kaplan, D. L., Silk-based biomaterials. Biomaterials 2003, 24 (3), 401-416. 42. Mosesson, M. W., Fibrin polymerization and its regulatory role in hemostasis. J Lab Clin Med 1990, 116 (1), 8-17. 43. Dunn, C. J.; Goa, K. L., Fibrin Sealant. Drugs 1999, 58 (5), 863-886. 44. Shaikh, F. M.; Callanan, A.; Kavanagh, E. G.; Burke, P. E.; Grace, P. A.; McGloughlin, T. M., Fibrin: A Natural Biodegradable Scaffold in Vascular Tissue Engineering. Cells Tissues Organs 2008, 188 (4), 333-346. 45. Mol, A.; van Lieshout, M. I.; Dam-de Veen, C. G.; Neuenschwander, S.; Hoerstrup, S. P.; Baaijens, F. P. T.; Bouten, C. V. C., Fibrin as a cell carrier in cardiovascular tissue engineering applications. Biomaterials 2005, 26 (16), 3113-3121. 46. Ye, Q.; Zünd, G.; Benedikt, P.; Jockenhoevel, S.; Hoerstrup, S. P.; Sakyama, S.; Hubbell, J. A.; Turina, M., Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering. European Journal of Cardio-Thoracic Surgery 2000, 17 (5), 587-591. 47. Hu, X.; Cebe, P.; Weiss, A. S.; Omenetto, F.; Kaplan, D. L., Protein-based composite materials. Materials Today 2012, 15 (5), 208-215. 48. Huang, C.; Chen, R.; Ke, Q.; Morsi, Y.; Zhang, K.; Mo, X., Electrospun collagen–chitosan–TPU nanofibrous scaffolds for tissue engineered tubular grafts. Colloids and Surfaces B: Biointerfaces 2011, 82 (2), 307-315. 49. Fraser, R. D.; MacRae, T. P., Intermediate filament structure. Biosci Rep 1985, 5 (7), 573-9. 50. Crewther, W. G.; Fraser, R. D.; Lennox, F. G.; Lindley, H., The chemistry of keratins. Adv Protein Chem 1965, 20, 191-346. 51. Hill, P.; Brantley, H.; Van Dyke, M., Some properties of keratin biomaterials: kerateines. Biomaterials 2010, 31 (4), 585-93. 52. Wang, B.; Yang, W.; McKittrick, J.; Meyers, M. A., Keratin: Structure, mechanical properties, occurrence in biological organisms, and efforts at bioinspiration. Prog Mater Sci 2016, 76, 229-318. 53. Coulombe, P. A.; Omary, M. B., 'Hard' and 'soft' principles defining the structure, function and regulation of keratin intermediate filaments. Current Opinion in Cell Biology 2002, 14 (1), 110-122. 54. Wawersik, M.; Coulombe, P. A., Forced expression of keratin 16 alters the adhesion, differentiation, and migration of mouse skin keratinocytes. Mol Biol Cell 2000, 11 (10), 3315-3327. 55. Magin, T. M.; Vijayaraj, P.; Leube, R. E., Structural and regulatory functions of keratins. Experimental Cell Research 2007, 313 (10), 2021-2032. 56. Powell, B. C.; Beltrame, J. S., Characterization of a Hair (Wool) Keratin Intermediate Filament Gene Domain. J Invest Dermatol 1994, 102 (2), 171-177. 57. Thibaut, S.; Barbarat, P.; Leroy, F.; Bernard, B. A., Human hair keratin network and curvature. International Journal of Dermatology 2007, 46, 7-10. 58. Sierpinski, P.; Garrett, J.; Ma, J.; Apel, P.; Klorig, D.; Smith, T.; Koman, L. A.; Atala, A.; Van Dyke, M., The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. Biomaterials 2008, 29 (1), 118-128. 59. Bellis, S. L., Advantages of RGD peptides for directing cell association with biomaterials. Biomaterials 2011, 32 (18), 4205-4210. 60. Wu, Y. L.; Lin, C. W.; Cheng, N. C.; Yang, K. C.; Yu, J., Modulation of keratin in adhesion, proliferation, adipogenic, and osteogenic differentiation of porcine adipose-derived stem cells. J Biomed Mater Res B Appl Biomater 2017, 105 (1), 180-192. 61. Lin, C. W.; Yang, K. C.; Cheng, N. C.; Tsai, W. B.; Lou, K. L.; Yu, J. S., Evaluation of adhesion, proliferation, and differentiation of human adipose-derived stem cells on keratin. J Polym Res 2018, 25 (2). 62. Wayner, E. A.; Kovach, N. L., Activation-Dependent Recognition by Hematopoietic-Cells of the Ldv Sequence in the V-Region of Fibronectin. J Cell Biol 1992, 116 (2), 489-497. 63. Saul, J. M.; Ellenburg, M. D.; de Guzman, R. C.; Van Dyke, M., Keratin hydrogels support the sustained release of bioactive ciprofloxacin. J Biomed Mater Res A 2011, 98 (4), 544-53. 64. de Guzman, R. C.; Saul, J. M.; Ellenburg, M. D.; Merrill, M. R.; Coan, H. B.; Smith, T. L.; Van Dyke, M. E., Bone regeneration with BMP-2 delivered from keratose scaffolds. Biomaterials 2013, 34 (6), 1644-56. 65. Silva, R.; Singh, R.; Sarker, B.; Papageorgiou, D. G.; Juhasz, J. A.; Roether, J. A.; Cicha, I.; Kaschta, J.; Schubert, D. W.; Chrissafis, K.; Detsch, R.; Boccaccini, A. R., Hybrid hydrogels based on keratin and alginate for tissue engineering. Journal of Materials Chemistry B 2014, 2 (33), 5441-5451. 66. Yue, K.; Liu, Y. H.; Byambaa, B.; Singh, V.; Liu, W. J.; Li, X. Y.; Sun, Y. X.; Zhang, Y. S.; Tamayol, A.; Zhang, P. H.; Ng, K. W.; Annabi, N.; Khademhosseini, A., Visible light crosslinkable human hair keratin hydrogels. Bioeng Transl Med 2018, 3 (1), 37-48. 67. Barati, D.; Kader, S.; Shariati, S. R. P.; Moeinzadeh, S.; Sawyer, R. H.; Jabbari, E., Synthesis and Characterization of Photo-Cross-Linkable Keratin Hydrogels for Stem Cell Encapsulation. Biomacromolecules 2017, 18 (2), 398-412. 68. Kim, I. Y.; Seo, S. J.; Moon, H. S.; Yoo, M. K.; Park, I. Y.; Kim, B. C.; Cho, C. S., Chitosan and its derivatives for tissue engineering applications. Biotechnol. Adv. 2008, 26 (1), 1-21. 69. Li, Z.; Ramay, H. R.; Hauch, K. D.; Xiao, D.; Zhang, M., Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials 2005, 26 (18), 3919-28. 70. Riva, R.; Ragelle, H.; des Rieux, A.; Duhem, N.; Jerome, C.; Preat, V., Chitosan and Chitosan Derivatives in Drug Delivery and Tissue Engineering. In Chitosan for Biomaterials II, Jayakumar, R.; Prabaharan, M.; Muzzarelli, R. A. A., Eds. Springer-Verlag Berlin: Berlin, 2011; Vol. 244, pp 19-44. 71. Muzzarelli, R.; Baldassarre, V.; Conti, F.; Ferrara, P.; Biagini, G.; Gazzanelli, G.; Vasi, V., Biological activity of chitosan: ultrastructural study. Biomaterials 1988, 9 (3), 247-252. 72. Chandy, T.; Sharma, C. P., Chitosan--as a biomaterial. Biomaterials, artificial cells, and artificial organs 1990, 18 (1), 1-24. 73. Sugimoto, M.; Morimoto, M.; Sashiwa, H.; Saimoto, H.; Shigemasa, Y., Preparation and characterization of water-soluble chitin and chitosan derivatives. Carbohydrate Polymers 1998, 36 (1), 49-59. 74. Francis Suh, J. K.; Matthew, H. W. T., Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials 2000, 21 (24), 2589-2598. 75. Lahiji, A.; Sohrabi, A.; Hungerford, D. S.; Frondoza, C. G., Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes. Journal of biomedical materials research 2000, 51 (4), 586-95. 76. Sukarto, A.; Yu, C.; Flynn, L. E.; Amsden, B. G., Co-delivery of adipose-derived stem cells and growth factor-loaded microspheres in RGD-grafted N-methacrylate glycol chitosan gels for focal chondral repair. Biomacromolecules 2012, 13 (8), 2490-502. 77. Knight, D. K.; Shapka, S. N.; Amsden, B. G., Structure, depolymerization, and cytocompatibility evaluation of glycol chitosan. J Biomed Mater Res A 2007, 83 (3), 787-98. 78. Uchegbu, I. F.; Sadiq, L.; Arastoo, M.; Gray, A. I.; Wang, W.; Waigh, R. D.; Schätzleinä, A. G., Quaternary ammonium palmitoyl glycol chitosan—a new polysoap for drug delivery. International Journal of Pharmaceutics 2001, 224 (1–2), 185-199. 79. Chen, Z.; Zhao, M.; Liu, K.; Wan, Y.; Li, X.; Feng, G., Novel chitosan hydrogel formed by ethylene glycol chitosan, 1,6-diisocyanatohexan and polyethylene glycol-400 for tissue engineering scaffold: in vitro and in vivo evaluation. Journal of materials science. Materials in medicine 2014, 25 (8), 1903-13. 80. Amsden, B. G.; Sukarto, A.; Knight, D. K.; Shapka, S. N., Methacrylated glycol chitosan as a photopolymerizable biomaterial. Biomacromolecules 2007, 8 (12), 3758-66. 81. Oberoi, G.; Nitsch, S.; Edelmayer, M.; Janjić, K.; Müller, A. S.; Agis, H., 3D Printing—Encompassing the Facets of Dentistry. Frontiers in Bioengineering and Biotechnology 2018, 6 (172). 82. Zhu, W.; Ma, X.; Gou, M.; Mei, D.; Zhang, K.; Chen, S., 3D printing of functional biomaterials for tissue engineering. Current Opinion in Biotechnology 2016, 40, 103-112. 83. Donderwinkel, I.; van Hest, J. C. M.; Cameron, N. R., Bio-inks for 3D bioprinting: recent advances and future prospects. Polymer Chemistry 2017, 8 (31), 4451-4471. 84. Gasperini, L.; Mano, J. F.; Reis, R. L., Natural polymers for the microencapsulation of cells. Journal of The Royal Society Interface 2014, 11 (100), 20140817. 85. Gorgieva, S.; Kokol, V., Preparation, characterization, and in vitro enzymatic degradation of chitosan‐gelatine hydrogel scaffolds as potential biomaterials. Journal of Biomedical Materials Research Part A 2012, 100 (7), 1655-1667. 86. Lu, M.; Liu, Y.; Huang, Y.-C.; Huang, C.-J.; Tsai, W.-B., Fabrication of photo-crosslinkable glycol chitosan hydrogel as a tissue adhesive. Carbohyd Polym 2018, 181, 668-674. 87. Lin, H.; Zhang, D.; Alexander, P. G.; Yang, G.; Tan, J.; Cheng, A. W.; Tuan, R. S., Application of visible light-based projection stereolithography for live cell-scaffold fabrication with designed architecture. Biomaterials 2013, 34 (2), 331-9. 88. Field, C. K.; Kerstein, M. D., Overview of wound healing in a moist environment. The American Journal of Surgery 1994, 167 (1, Supplement), S2-S6. 89. Peng, H. T.; Martineau, L.; Shek, P. N., Hydrogel–elastomer composite biomaterials: 1. Preparation of interpenetrating polymer networks and in vitro characterization of swelling stability and mechanical properties. Journal of Materials Science: Materials in Medicine 2007, 18 (6), 975-986. 90. Rahmany, M. B.; Hantgan, R. R.; Van Dyke, M., A mechanistic investigation of the effect of keratin-based hemostatic agents on coagulation. Biomaterials 2013, 34 (10), 2492-2500. 91. Burnett, L. R.; Rahmany, M. B.; Richter, J. R.; Aboushwareb, T. A.; Eberli, D.; Ward, C. L.; Orlando, G.; Hantgan, R. R.; Van Dyke, M. E., Hemostatic properties and the role of cell receptor recognition in human hair keratin protein hydrogels. Biomaterials 2013, 34 (11), 2632-2640. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58017 | - |
dc.description.abstract | 燒燙傷是一個嚴重的全球健康議題,目前全球每年有18萬人因燒燙傷而死亡。為了解決這項問題,科學家發展了各式各樣的人工皮膚以及替代物。其中,水凝膠型態的組織支架對皮膚傷口復原是一個具有前景的選擇,不只是因為它能夠提供濕潤的環境促進傷口修復,同時它也能吸收傷口所滲出的血液、組織液等。在這項研究中,我們使用了兩種不同的天然高分子來製作能夠透過照射紫外光交聯的水凝膠,以作為皮膚組織工程的相關應用。
角蛋白是富含於自然界中的一種結構性蛋白,常見於頭髮、指甲和羽毛等生物組織中,其胺基酸序列組成具有許多對細胞友善的利基,能夠和細胞膜上的受體交互作用,促進貼附和生長等現象,同時也具有良好的生物降解性,因此在過去常被用於製作生醫材料,然而因為其固有的雙硫鍵結構,使其的可加工性受到很大的侷限。透過化學萃取方式,我們將人類頭髮中的角蛋白萃取出來,並經由化學改質,使其具備水溶性與紫外光交聯等特性,以將其製備成水凝膠。 幾丁質是常見於節肢動物外骨骼中的成分,透過處理能夠將其製成甲殼素,應用於工業、醫藥與美容等領域,其特點在於具有良好的生物相容性以及生物降解性,以及作為調整機械性質的材料,此外其特有的陰電性質也被指出具有誘導幹細胞進行軟骨分化的能力。實驗中我們使用水溶性甲殼素,並對其做化學修飾使其具備紫外光交聯的性質,以用於光交聯水膠之開發。 在此研究中,藉由將修飾過的角蛋白和修飾後之水溶性甲殼素混合,製備出具有快速交聯能力和理想機械強度的水凝膠。過程中,我們將探討萃取出的角蛋白之性質、對角蛋白和甲殼素進行化學修飾的效果,使用紫外光使水凝膠交聯並評估水凝膠的機械強度和降解速度等。為了調查細胞在水凝膠的表現,我們將細胞種植在水凝膠表面,觀察細胞貼附、生長能力還有細胞毒性的測試。 | zh_TW |
dc.description.abstract | Burns are a crucial global health problem. An estimated 180,000 deaths are caused by burns annually. To prepare the antidote for this problem, scientists develop various artificial skin and skin substitutes. A hydrogel system is a promising candidate for skin wound healing. Not only because of its high water-content to offer a moist environment for healing, but also its ability to absorb the blood and the extracellular fluid in the wound site. In this study, we utilized different natural polymers as materials to fabricate a UV-crosslinkable hydrogel system for skin tissue engineering.
Keratin is a kind of structural protein common in animal tissues, such as hair, nail, feather, and so on. Due to the excellent biodegradability and biocompatibility, keratin was frequently used as biomaterials. The cell-binding motifs in its peptide sequences, such as leucine-aspartic acid-valine (LDV) and glutamic acid-aspartic acid-serine (EDS), can interact with receptors on cell membranes to promote cell adhesion and proliferation. Nonetheless, because of its intrinsic disulfide bond structure, its processability has significantly been constrained. In this study, we extracted keratin from human hair with the chemical method. Further, we chemically modify the keratin to make it water-soluble and UV-crosslinkable. Chitosan, a kind of polysaccharide, is made from chitin shells of the crustaceans. Its characteristics include outstanding biocompatibility, biodegradability, and simple to control the mechanical strength. Furthermore, its unique electronegativity is indicated to lead stem cell to chondrogenic differentiation. Therefore, in developing a UV- triggered hydrogel system, we choose water-soluble chitosan (glycol chitosan) as the other supporting material, and then chemically modify it to become UV-crosslinkable. In this research, we combined the chemically modified keratin and glycol chitosan to fabricate a fast crosslinking hydrogel with ideal mechanical strength. In the processes, the characteristics of extracted keratin were assessed. The degree of grafting was also measured. Further, we examined the surface morphology, the mechanical strength and the speed of degradation. To investigate the biocompatibility, we cultured L929 cells on hydrogel surfaces to observe the performance of the cell adhesion, proliferation, and the cytotoxicity. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T08:04:36Z (GMT). No. of bitstreams: 1 U0001-1507202017101000.pdf: 3483810 bytes, checksum: 0c1c77ac6c57ade09d980569bb0c0788 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 摘要 i Abstract ii Contents iv List of Figures vii List of Tables x Chapter 1 Introduction 1 1.1 Tissue Engineering 1 1.1.1 Overview 1 1.1.2 Skin Tissue Engineering 2 1.1.3 Biomaterials in Skin Tissue Engineering 4 1.1.4 Introduction to Keratin 8 1.1.5 Keratin in Tissue Engineering 9 1.1.6 Chitosan as biomaterials 11 1.2 Bioprinting 12 1.2.1 3D printing 12 1.2.2 3D printing in Tissue Engineering 13 1.2.3 Bioink 15 1.3 Motivation and Aims 17 1.4 Research Framework 17 Chapter 2 Materials and Methods 19 2.1 Materials 19 2.1.1 Keratin Extraction 19 2.1.2 Chemical modification of keratin 19 2.1.3 Degree of grafting measurement 19 2.1.4 Hydrogel degradation test 20 2.1.5 Cell culture and Viability 20 2.2 Equipment 22 2.3 Solution formula 24 2.4 Methods 25 2.4.1 Keratin Extraction 25 2.4.2 SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) analysis 25 2.4.3 Solubilization of Extracted Keratin 26 2.4.4 Synthesis of Methacrylate Keratin 26 2.4.5 Synthesis of Methacrylate Glycol Chitosan 27 2.4.6 Measurement of Degree of Grafting (DOG) 28 2.4.7 UV-crosslinking Gelation Test 28 2.4.8 Gelation Time and Mechanical Test 29 2.4.9 Preparation of KE/GC (methacrylate keratin/methacrylate glycol chitosan) composite films 29 2.4.10 Cell culture on methacrylate glycol chitosan/methacrylate keratin hydrogel surface 30 2.4.11 Degradation test 31 2.4.12 Mass swelling 31 2.4.13 Statistical analysis 32 Chapter 3 Results and discussion 37 3.1 Characteristics of human hair keratin 37 3.2 Characteristics of chemically modified keratin and glycol chitosan 38 3.2.1 Keratin Solubilization 38 3.2.2 Chemical Modification and Degree of Grafting for Glycol Chitosan 38 3.2.3 Gelation test of GCKE hydrogels 39 3.2.4 Swelling and Stability of GCKE Hydrogels 39 3.2.5 Rheological Properties 40 3.2.6 Scanning electron microscope (SEM) image of UV-crosslinking hydrogels 41 3.3 In vitro study of UV-crosslinking Hydrogel 42 3.3.1 Cell Morphology (2D Cell Culture) 42 3.3.2 Cell Viability (2D Cell Culture) 42 Conclusion 61 Future Perspective 62 References 63 | |
dc.language.iso | en | |
dc.title | 紫外光交聯之幾丁聚醣衍生物與角蛋白的複合式水凝膠於組織工程的發展 | zh_TW |
dc.title | UV-Crosslinkable Glycol Chitosan/Keratin Composite Hydrogel for Tissue Engineering | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 廖英志(Ying-Chih Liao),李亦宸(Yi-Chen Li) | |
dc.subject.keyword | 角蛋白,甲殼素,水凝膠,3D列印,生物墨水,UV光交聯,微組織, | zh_TW |
dc.subject.keyword | keratin,glycol chitosan,UV light crosslinking,hydrogel,bioprinting,microtissue, | en |
dc.relation.page | 69 | |
dc.identifier.doi | 10.6342/NTU202001550 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-08-03 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
U0001-1507202017101000.pdf 目前未授權公開取用 | 3.4 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。