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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林江珍(Jiang-Jen Lin) | |
| dc.contributor.author | Yi-Hsiu Huang | en |
| dc.contributor.author | 黃詣琇 | zh_TW |
| dc.date.accessioned | 2021-06-16T07:07:37Z | - |
| dc.date.available | 2019-08-11 | |
| dc.date.copyright | 2014-08-11 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-07-09 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57854 | - |
| dc.description.abstract | 醫學科技日新月異，表面改質技術的開發使醫療植入物的功能與效用從以往單純之機械性質導向，提升至今日兼具生物相容性之需求層面發展。骨科鋼板手術的最大併發症是發生感染並導致骨髓炎，因此具有抗菌表面之金屬能有效降低手術中的感染風險。由奈米銀粒子 (AgNP) 、層狀結構之矽片 (NSP) 、水性PU所組成之三成分奈米複合材料均勻分散於水中，塗佈於骨科鋼板之表面後，能作為具有良好抗菌及附著之骨科植入物。在骨科醫療應用上，不鏽鋼廣泛使用於骨折支架等用途，因此在此金屬表面進行改質，使之具有抗菌效果，為本篇研究之目的。
在此研究中，由之前已開發之奈米矽片銀 (AgNP/NSP 7/93) 均勻地與水性PU 依據不同的組成比例 (1/0.1至1/10 w/w) 進行混合，由紫外光吸收與穿透式電子顯微鏡等觀察，發現奈米銀粒子穩定且其大小約7~11 nm。於實驗中薄膜厚度約為1.5 μm，且發現表面抗菌性與薄膜厚度無關，但與組成中矽片銀含量有關，水性PU於系統中作為附著劑(Adhesion agent)，並不影響奈米銀粒子之大小與成膜後之抗菌效果，在矽片銀混水性PU比例1/1~1/5 (w/w)中，具有良好的抗菌效果，而在毒性測試 (Mouse fibroblasts) 中，更凸顯材料的低毒特性。本研究針對不同組成比例成膜後之抗菌性、附著度以及對細菌所產生之影響，以及作為骨科植入物作研究探討，希望日後有機會做為造福人群之生醫材料。 | zh_TW |
| dc.description.abstract | Metallic implants are highly associated with osteomyelitis happening. Antibacterial surface is one of the strategies to approach this problem. A tri-component nanohybrid dispersion in water comprising of silver nanoparticles (AgNP), nanometer-thick silicate platelets (NSP) and waterborne polyurethane (PU) was developed for surface coating on orthopedic metal plates. Since the stainless metal plates are commonly used as the implants to repair bond fractures in the orthopedic field, it is desired to functionalize the metal surface with antimicrobial property.
In this study, the previously developed AgNP-on-NSP nanohybrid was homogeneously blended into a selected waterborne PU dispersion at varied weight ratios from 1/0.1 to 1/10 (w/w). The resultant dispersions were shown to have stable AgNP at the size of 7-12 nm in diameter, characterized by using UV absorption and Transmission electron microscope (TEM) direct observations. The subsequent coating process allowed the formation of a nanohybrid thin film on the metal plate surface. It was found that the antimicrobial efficacy was irrelevant to the thickness of the coated materials but closely depending on the composition of Ag in PU. The presence of PU was essential for adhering Ag onto the metal surface, however, avoidably reducing the antimicrobial efficacy due to the dilution effect. Both functions were compromised by judiciously selecting the composition of AgNP-NSP to PU in the range from 1/1 to 1/5 (w/w). Furthermore, the metal plate coated with the specific 1/3 (w/w) composition was shown to be low cytotoxicity towards the contacted mouse fibroblasts. Overall, the uses of tri-component Ag/silicate/PU in water dispersion could generate a scratch-free thin film (thickness about 1.5 μm) on the metal surface while exhibiting both of antimicrobial and biocompatible properties. The facile coating technique of the AgNP in waterborne PU is proven to be viable for fabricating infection-free medical devices. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T07:07:37Z (GMT). No. of bitstreams: 1 ntu-103-R01549012-1.pdf: 3317600 bytes, checksum: 74eb9998cb303b16e7614c8b84028537 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
Acknowledgements II 摘要 III Abstract V Contents VII Figure Captions IX Table Captions XI Chapter 1 Introduction and literature review 1 1.1 Introduction of Nanomaterials 1 1.2 Introduction of nanoscale silicate platelets (NSP) 3 1.3 Introduction of slilver nanoparticles (AgNP) 5 1.3.1 Method of preparing silver nanoparticles (AgNP) 6 1.3.2 Silver as antibacterial agent 9 1.3.3 Bactericidal mechanism of silver 10 1.3.3.1 Bactericidal mechanism of silver ions 10 1.3.3.2 Bactericidal mechanism of silver nanoparticles 11 1.4 Water borne polyurethane (PU) 12 1.5 The risk of surgical-wound infection 13 1.6 Research objectives 14 Chapter 2 Experimental section 16 2.1 Materials 16 2.2 Synthesis of AgNP/NSP 7/93 nanohybrids 17 2.3 Synthesis of the waterborne PU 17 2.4 Solution coating of AgNP/NSP-PU on metal plates 18 2.5 Adhesion property of AgNP/NSP-PU film75 19 2.6 Antibacterial efficacy of AgNP/NSP-PU nanohybrid coated surface 19 2.6.1 Using phosphate buffered saline as dilute solution: 20 2.6.2 Using Luria Bertani as dilute solution: 20 2.7 Antibacterial efficacy of AgNP/NSP-PU films by LIVE/DEAD analysis 21 2.8 Biofilm assay and SEM of AgNP/NSP-PU surface 21 2.9 Cytotoxicity test of AgNP/NSP-PU surface 22 2.10 Instruments and Analyses 24 Chapter 3 Results and Discussion 26 3.1 Synthesis of AgNP/NSP 7/93 nanohybrids 26 3.2 The adhesion agent in the nanohybrid system 29 3.3 Physical property of AgNP/NSP-PU on stainless steel surface 30 3.4 AFM analysis of AgNP/NSP-PU surface 33 3.5 EDS analysis of AgNP/NSP-PU surface 34 3.6 Antibacterial efficacy of AgNP/NSP-PU and SEM analyses 36 3.6.1 Solution type ─ Antibacterial efficacy of AgNP/NSP-PU 36 3.6.2 Film type ─ Antibacterial efficacy of AgNP/NSP-PU 38 3.6.2.1 Using phosphate buffered saline as dilute solution: 38 3.6.2.2 Using Luria Bertani (LB) as dilute solution: 41 3.7 Antibacterial efficacy of AgNP/NSP-PU films by LIVE/DEAD analysis 43 3.8 Biofilm assay and SEM of AgNP/NSP-PU surface 44 3.9 Cytotoxicity test of the AgNP/NSP-PU dispersion 46 Chapter 4 Conclusion 48 Chapter 5 References 49 | |
| dc.language.iso | en | |
| dc.title | 奈米矽片銀/PU附著骨科用鋼材表面之抗菌與生物相容性探討 | zh_TW |
| dc.title | Antimicrobial Function and Biocompatibility of Orthopedic Steel Plates by Thin-Film Coating of Tri-Component Silver-on-Silicate Platelet/PU Nanohybrids | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 謝國煌,張志豪,胡淑文,劉定宇(Ting-Yu Liu) | |
| dc.subject.keyword | 奈米矽片,奈米銀粒子,水性樹脂,抗菌性,附著,醫療器材,不鏽鋼,表面改質,塗佈, | zh_TW |
| dc.subject.keyword | nanosilicate platelets (NSP),silver nanoparticles (AgNP),waterborne polyurethane (PU),antibacterial,adhesion,medical device,stainless plate,surface treatment,coating, | en |
| dc.relation.page | 55 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-07-09 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
| 顯示於系所單位： | 高分子科學與工程學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-103-1.pdf 目前未授權公開取用 | 3.24 MB | Adobe PDF |
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