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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃光裕(Kuang-Yuh Huang) | |
dc.contributor.author | Haw Hsiao | en |
dc.contributor.author | 蕭皓 | zh_TW |
dc.date.accessioned | 2021-06-16T07:07:23Z | - |
dc.date.available | 2020-07-21 | |
dc.date.copyright | 2020-07-21 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-07-16 | |
dc.identifier.citation | [1] LEDs magazine 數位微反射鏡陣列構造解析相關網頁https://www.ledsmagazine.com/leds-ssl-design/microcontrollers/article/16695785/digital-micromirror-devices-enable-dynamic-stage-lighting-magazine [2] Dana, D., Walter, D., and John, S., “Emerging Digital micromirror device (DMD) applications”, School psychology international., 2003, Vol.4985, p.14 [3] Isabel, R. and João, S., “Cell nucleus GFP flow analysis from sequences of LSCFM images”, Proc. Of Portugal-UT Austin CFD., 2008 [4] Manish, S., Gangadhar E., and Sai S. G., “Structured illumination microscopy”, Advances in optics and photonics., 2015, Vol. 7, Issue. 2, p. 241-275 [5] Daryl, L., Kengyeh K. C., and Jerome M., “Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy”, Optics letters, 2008, Vol. 33, Issue. 16, p.1819-1821 [6] Silvia, S., Kengyeh K. C., Nenad B., Timothy N. F., Claire, H., Aaron, C. B., Satish K. S., and Jerome, M., “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle”, Journal of Biomedical Optics, 2009, Vol. 14, Issue. 3, 030502 [7] Daryl, L., Tim, N. F., Kengyeh, K. C., and Jerome M., “Optically sectioned in vivo imaging with speckle illumination HiLo microscopy”, Journal of Biomedical Optics, 2011, Vol. 16, Issue. 1, 016014 [8] Timothy, N. F., Daryl, L., and Jerome, M., “Fast optically sectioned fluorescence HiLo endomicroscopy”, Journal of Biomedical Optics, 2012, Vol. 17, Issue. 2, 021105 [9] Chen-Yen, L., Wei-Hsin, L., Ju-Hsuan, C., Jui-Chang, T., and Yuan, L., “In vivo volumetric fluorescence sectioning microscopy with mechanical-scan-free hybrid illumination imaging”, Biomedical Optics Express, 2016, Vol. 7, Issue. 2 p.3968-3978 [10] W. T. Lin, C. Y. Lin, V. R. Singh, and Y. Luo, 'Speckle illumination holographic non-scanning fluorescence endoscopy,' Journal of Biophotonics, 2018, Vol. 7, Issue. 2 p.3968-3978 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57850 | - |
dc.description.abstract | 螢光內視鏡能提供高對比生物影像,因此廣泛應用於生醫領域。然而,傳統螢光內視鏡缺少深度解析能力,因此很難從厚生物樣本獲得高解析三維影像。近年來光學切片內視鏡的研究蓬勃發展,各種光學切片法相繼被提出,但還存在待克服的限制。共焦內視鏡有良好的切片能力與橫向解析度,但需要冗長的橫向掃描,成像速度有限;結構光內視鏡無需橫向掃描,但存在結構光相移誤差的問題;紙片光內視鏡成像速度高、無須重建,但有不均勻照明光、空間限制影像最高解析度、複雜系統等缺點。相較於早期開發光學切片系統,高低頻(HiLo)光學切片內視鏡僅需兩幀即可重建出光學切片影像,且系統架構以螢光顯微鏡為基礎,相對簡單。為了產生全空間頻率之光學切片影像並重建生物樣本三維螢光分布,本論文結合微反射鏡裝置產生高低頻光學切片法所需柵狀結構照明,電控可變焦透鏡進行定放大率之軸向掃描,搭配NA= 0.26之自設計內視鏡探頭,達成30 μm之光學切片能力,同時掃描範圍長達810 μm。為了全面性驗證系統之光學切片效能,對標準螢光球、螢光花粉、老鼠心臟肌肉組織、老鼠小腸組織以所開發可調高低頻光學切片內視鏡進行了實際拍攝測試。 | zh_TW |
dc.description.abstract | Fluorescence endo-microscopy is widely used in biomedical field due to its capability to provide biological images with high contrast. However, conventional fluorescence microscopy is lack of depth resolving power. In recent years, research about optically sectioning endo-microscopy has been flourishing. Several optical sectioning technique has been presented, all of which have their own limitation, though. Confocal endo-microscopy has strong depth discrimination power, but its acquisition time is relatively long due to requirement of lateral scanning. Structured light endo-microscopy, a kind of wide-field optical sectioning microscopy, doesn't require lateral scanning, but it faces problems of phase difference error between structured illumination images. Light sheet endo-microscopy has high acquisition rate but has disadvantages such as nonuniform illumination, resolution limitation due to orthogonal setup, complicated system. Compared to above optically sectioning techniques, HiLo endo-microscopy only requires two frames to reconstruct an optically sectioned image, and its structure is based on fluorescence endo-microscopy, which is relatively simple. To obtain an optical -ly sectioned image with full spatial resolution and reconstruct 3-dimensional (3D) fluorescence distribution of biological samples, the thesis combined digital micro-mirror device to generate grid illumination required by HiLo optical sectioning algorithm, electrically tunable lens to achieve axial scanning with invariant resolution and magnification, and custom-made NA = 0.26 endoscopic probe. The optical sectioning capability is measured to be 30 μm, and axial scanning range is 810 μm. In order to fully demonstrate the optical sectioning performance, standard fluorescent beads, fluorescent pollens, mice heart tissues, mice intestine tissues are imaged using the developed endoscope. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T07:07:23Z (GMT). No. of bitstreams: 1 U0001-1607202017222100.pdf: 5811334 bytes, checksum: ebfd5d024d2a55231768789d2ae75e28 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 目錄 致謝 I 摘要 II Abstract III 目錄 IV 圖目錄 VI 表目錄 VIII 符號表 IX 第一章 緒論 1 1.1 研究背景與動機 1 1.2 文獻回顧 3 1.2.1數位微反射鏡陣列 3 1.2.2 共焦切片內視鏡 4 1.2.3 結構光切片內視鏡 6 1.2.4 高低頻切片顯微術 8 1.3 研究目標 11 1.4 內容簡介 12 第二章 高低頻光學切片內視鏡原理與光學系統設計 13 2.1 高低頻影像處理法之原理 13 2.1.1 高頻之焦平面影像擷取 14 2.1.2 低頻之焦平面影像擷取 15 2.1.3 對比分布計算:居部對比法 15 2.1.4 對比分布計算:差分法 16 2.1.5 光學切片能力之優化:二維帶通濾波器 17 2.2 高低頻光學切片內視鏡架構 20 2.3 結構光之創造:數位微反射鏡陣列 21 2.3.1 數位微反射鏡之工作原理與架設 21 2.3.2 柵狀結構照明 23 2.3.3 繞射特性 27 2.4 軸向掃描裝置:電控可變焦透鏡 28 2.4.1 電控變焦透鏡工作原理 28 2.4.2 等放大率之軸向掃描 28 2.4.3 延長透鏡組之選用 29 2.4.4 有效焦長之計算 31 2.5 理論擷取時間之計算 34 2.6 內視鏡探頭之結構 35 2.7 自動化拍攝程序流程 36 第三章 內視鏡效能驗證 37 3.1 橫向解析度之理論與驗證 37 3.2 視場尺寸量測 40 3.3 調製轉移函數量測 41 第四章 軸向掃描實驗驗證 43 4.1 軸向掃描校正曲線 43 4.2 軸向掃描等放大率之實驗驗證 44 第五章 光學切片能力之實驗與驗證 45 5.1 光學切片能力量測 45 5.2 標準螢光球影像處理與分析 47 5.3 螢光花粉粒影像處理與分析 49 5.4 老鼠心臟肌肉組織切片影像處理與分析 50 5.5 老鼠小腸組織影像處理與分析 51 5.6 帶通濾波器參數優化 53 第六章 可攜化內視鏡系統之設計 56 第七章 結論與未來展望 57 參考文獻 58 附錄A 數位微反射鏡陣列規格 60 附錄B CCD相機規格 61 附錄C 電控可變焦透鏡規格 62 附錄D 雷射光源規格 63 附錄E 內視鏡探頭規格 64 附錄F 實體光柵規格 67 附錄G 線性致動器規格 68 | |
dc.language.iso | zh-TW | |
dc.title | 可調高低頻光學切片內視鏡用於三維螢光成像 | zh_TW |
dc.title | Tunable HiLo optical sectioning endoscopy for volumetric fluorescence imaging | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.author-orcid | 0000-0002-5890-9212 | |
dc.contributor.advisor-orcid | 黃光裕(0000-0002-2981-4986) | |
dc.contributor.coadvisor | 駱遠(Yuan Luo) | |
dc.contributor.coadvisor-orcid | 駱遠(0000-0001-9776-7897) | |
dc.contributor.oralexamcommittee | 林沛群(Pei-Chun Lin),廖先順(Hsien-Shun Liao) | |
dc.contributor.oralexamcommittee-orcid | 林沛群(0000-0001-9146-3817),廖先順(0000-0003-1338-0332) | |
dc.subject.keyword | 光學切片內視鏡,全場式光學系統,數位微反射鏡陣列,電控可變焦透鏡, | zh_TW |
dc.subject.keyword | Optical sectioning endoscopy,wide-field optical system,DMD,Electrically tunable lens, | en |
dc.relation.page | 68 | |
dc.identifier.doi | 10.6342/NTU202001578 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-07-17 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 機械工程學研究所 | zh_TW |
顯示於系所單位: | 機械工程學系 |
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