請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57623
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曾顯雄(Shean-Shong Tzean) | |
dc.contributor.author | Dan-Jung Lee | en |
dc.contributor.author | 李丹容 | zh_TW |
dc.date.accessioned | 2021-06-16T06:54:38Z | - |
dc.date.available | 2019-12-31 | |
dc.date.copyright | 2014-08-21 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-07-21 | |
dc.identifier.citation | 白佳真。2003。樟芝免疫調節蛋白與超氧歧化酶之研究。國立台灣海洋大學生物科技研究所碩士論文。
高曉薇。1991。台灣靈芝屬新種樟芝之三萜類成分研究。台北醫學院天然物醫學研究所碩士論文。 莊榮輝。2000。酵素化學實驗。台灣大學農化系。頁76~81。 曹巧吟。2003。樟芝中免疫調節蛋白的純化與其生理活性之探討。國立台灣大學園藝學研究所碩士論文。 張怡婷。2004。樟芝抗腫瘤活性之研究。國立成功大學藥理學研究所碩士論文。 張中姿。2002。樟芝菌絲體之甲醇萃取部分對人類肝癌細胞株 (HepG2) 生長抑制作用的機轉探討。國立台灣大學醫學院生物化學暨分子生物學研究所碩士論文。 游慧娟。2006。深層發酵樟芝菌絲體乙醇萃取物對人類肺癌及肝癌細胞生長之影響與其作用機轉之探討。國立台灣大學食品科技研究所碩士論文。 黃鈴娟。1999。樟芝與姬松茸之抗氧化性質及其多醣組成分析。中興大學食品科學系碩士論文。 蔡雁暉。2002。樟芝深層培養液及其多醣體之抗氧化特性。國立中興大學食品科學系研究所碩士論文。 劉宇真。2007。牛樟芝之抗氧化酶相關基因選殖及其生理活性分析。台灣大學植物病理與微生物學系碩士論文。 盧祉彤。2004。牛樟芝菌體對腫瘤細胞的影響。南台科技大學生物科技研究所碩士論文。 賴鈺菁。2004。樟芝發酵液之抗發炎及其誘導癌細胞凋亡機制之探討。中國醫藥大學營養研究所碩士論文。 Alscher, R. G., Erturk, N., and Heath, L. S. 2002. Role of superoxide dismutase (SODs) in controlling oxidative stress in plants. J. Exp. Bot. 53: 1331-1341. Altschul, A. M., Abrams, R., and Hogness, T. R. 1940. Cytochrome c peroxidase. J. Biol. Chem. 241: 2983-2984. Ao, Z., Xu, Z., Lu, Z., Xu, H., Zhang, X., and Dou, W. 2009. Niuchangchih (Antrodia camphorata) and its potential in treating liver diseases. J. Ethnopharmacol. 121: 194-212. Arthur, J. R. 2000. The glutathione peroxidases. Cell. Mol. Life Sci. 57: 1825-1835. Bannister, J. V., Bannister, W. H., and Rotilio, G. 1987. Aspects of the structure, function, and applications of superoxide dismutase. CRC Crit. Rev. Biochem. 22: 111-180. Beauchamp, C., and Fridovich, I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-287. Bechtold, R., and Bosshard, H. R. 1985. Structure of an electron transfer complex II Chemical modification of carboxyl groups of cytochrome c peroxidase in presence and absence of cytochrome c. J. Biol. Chem. 260: 5191-5200. Berman, H. M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T. N., Weissig, H., Shindyalov, I. N., and Bourne, P. E. 2000. The Protein Data Bank. Nucleic Acids Res. 28: 235-42. Blokhina, O., Virolainen, E., and Fagerstedt, K. V. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Botany 91: 179-194. Bosshard, H. R., Bänziger J., Hasler, T., and Poulos, T. L. 1984. The cytochrome c peroxidase-cytochrome c electron transfer complex-The role of histidine residues. J. Biol. Chem. 259: 5683-5690. Boveris, A. 1976. Mitochondrial production of hydrogen peroxide in Saccharomyces cerevisiae. Acta Physiol. Lat. Am. 26: 303-309. Chang, E. C., Crawford, B. F., Hong, Z., Bilinski, T., and Kosman, D. J. 1991. Genetic and biochemical characterization of Cu, Zn superoxide dismutase mutants in Saccharomyces cerevisiae. J. Biol. Chem. 266: 4417-4424. Chang, T. T., and Chou, W. N. 2004. Antrodia cinnamomea reconsidered and A. salmonea sp. nov. on Cunninghamia konishii in Taiwan. Bot. Bull. Acad. Sin. 45: 347-352. Chang, T. T., and Chou, W. N. 1995. Antrodia cinnamomea sp. nov. on Cinnamomum kanehiral in Taiwan. Mycol. Res. 99: 756-758. Charizanis, C., Juhnke, H., Krems, B., and Entian, K. D. 1999. The oxidative stress response mediated via Pos9/Skn7 is negatively regulated by the Ras/PKA pathway in Saccharomyces cerevisiae. Mol. Genet. Genomics 261: 740–752. Chen, C. C., Shiao, Y. J., Lin, R. D., Shao, Y. Y., Lai, M. N., Lin, C. C., Ng, L. T., and Kuo, Y. H. 2006. Neuroprotective diterpenes from the fruiting body of Antrodia camphorate. J. Nat. Prod. 69: 689-691. Chen, C. H., Yang, S. W., and Shen, Y. C. 1995. New steroid acids from Antrodia cinnamomea, a fungal parasite of Cinnamomum micranthum. J. Nat. Prod. 58: 1655-1661. Chen, S. C., Lu, M. K., Cheng, J. J., and Wang D. L. 2005. Antiangiogenic activities of polysaccharides isolated from medicinal fungi. FEMS Microbiol. Lett. 249: 247-254. Cheng, J. J., Huang, N. K., Chang, T. T., Wang, D. L., and Lu, M. K. 2005. Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. Life Sci. 76: 3029-3042. Cherng, I. H., Chiang, H. C., Cheng, M. C., and Wang, Y. 1995. Three new triterpenoids from Antrodia cinnamomea. J. Nat. Prod. 58: 365-371. Choi, Y. M., Ku, J. B., Chang, H. B., and Lee, J. 2005. Antioxidant activities and total phenolics of ethanol extracts from several edible mushrooms produced in Korea. Food Sci. Biotechnol. 14: 700-703. Combet, C., Blanchet, C., Geourjon, C., and Deléage, G. 2000. NPS@: Network Protein Sequence Analysis. Trends Biochem. Sci. 25: 147-150. Czapski, J. 2004. Antioxidant activity and phenolic content in some strains of mushrooms (Agaricus bisporus). Veg. Crops Res. Bull. 62: 165-173. Darwin, S., Pablo, F., Alejandra, R., Edilia, A., and Angel, O. 2012. Oral limmunization of mice with recombinant Lactococcus lactis expressing Cu, Zn superoxide dismutase of Brucella abortus triggers protective immunity. Vaccine 30: 1283-1290. Ding, D., Liu, S., Wang, K., Huang, L., and Zhao, J. 2014. Expression, purification, and characterization of Cu/ZnSOD from Panax Ginseng. Molecules 19: 8112-8123. Douglas, M. G., McCammon, M. T., and Vassarotti, A. 1986. Targeting proteins into mitochondria. Microbiol. Rev. 50: 166-178. Dubost, N. J., Beelman, R. B., and Royse, D. J. 2007. Influence of selected cultural factors and postharvest storage on ergothioneine content of common button mushroom Agaricus bisporus (J. Lge) Imbach (Agaricomcetideae). Int. J. Med. Mushrooms 9: 163-176. Elmastas, M., Isaldak, O., Turkekul, I., and Temur, N. 2007. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J. Food Comp. Anal. 20: 337-344. Erman, J. E., Kilheeney, H., Bidwai, A. K., Ayala, C. E., and Vitello, L. B. 2013. Peroxygenase activity of cytochrome c peroxidase and three apolar distal heme pocket mutants: hydroxylation of 1-methoxynaphthalene. BMC Biochem. 14: 19. Erman, J. E., and Vitello, L. B. 2002. Yeast cytochrome c peroxidase: mechanistic studies via protein engineering. Biochim. Biophys. Acta 1957: 193-220. Fridovich, I. 1986a. Biological effects of the superoxide radical. Arch. Biochem. Biophys. 247: 1-11. Fridovich, I. 1986b. Superoxide dismutase. Adv. Enzymol. Relat. Areas Mol. Biol. 41: 61-97. Fridovich, I. 1995. Superoxide radical and superoxide dismutases. Annu. Rev. Biochem. 64: 97-112. Gasser, S. M., Ohashi, A., Daum, G., Bohni, P. C., Gibson, J., Reid, G. A., Yonetani, T., and Schatz, G. 1982. Proc. Natl. Acad. Sci. U.S.A. 79: 267-271. Geethangili, M., and Tzeng, Y. 2009. Review of pharmacological effects of Antrodia camphorata and its bioactive compounds. eCAM. 2011: 212641. Giles, S. S., Perfect, J. R., and Cox, G. M. 2005. Cytochrome c peroxidase contributes to the antioxidant defense of Cryptococcus neoformans. Fungal Genet. Biol. 42: 20-29. Grivell, L. A. 1988. Protein import into mitochondria. Int. Rev. Cytol. 111: 107-141. Halliwell, B. 1993. The role of oxygen radicals in human disease, with particular reference to the vascular system. Haemostasis 23: 118-126. Han, H. F., Nakamura N., Zuo, F., Hirakawa, A., Yokozawa, T., and Hattori, M. 2006. Protective effects of a neutral polysaccharide isolated from the mycelium of Antrodia cinnamomea on Propionibacterium acnes and lipopolysaccharide induced hepatic injury in mice. Chem. Pharm. Bull. 54: 496-500. Hemández-Saavedra N. Y. 2003. Cu, Zn superoxide dismutase in Rhodotorula and Udeniomyces spp. isolated from sea water: cloning and sequencing the encoding region. Yeast 20: 479-492. Hseu, Y., Chen, S., Yech, Y., Wang, L., and Yang, H. 2008. Antioxidant activity of Antrodia camphorata on free radical-induced endothelial cell damage. J. Ethnopharmacol. 118: 237-245. Hseu, Y. C., Chang, W. C., Hseu, Y. T., Lee, C. Y., Yech, Y. J., Chen, P. C., Chen, J. Y., and Yang, H. L. 2002. Protection of oxidative damage by aqueous extract from Antrodia camphorata mycelia in normal human erythrocytes. Life Sci. 71: 469-482. Hseu, Y. C., Wu, F. Y., Wu, J. J., Chen, J. Y., Chang, W. H., Lu, F. J., Lai, Y. C., and Yang, H. L. 2005. Anti-inflammatory potential of Antrodia camphorata through inhibition of iNOS, COX-2, and cytokines via the NF-κB pathway. Int. Immunopharmacol. 5: 1914-1925. Hsiao, G., Shen, M. Y., Lin, K. H., Lan, M. H., Wu, L. Y., Chou, D. S., Lin, C. H., Su, C. H., and Sheu, J. R. 2003. Antioxidative and hepatoprotective effects of Antrodia camphorata extract. J. Agrc. Food Chem. 51: 3302-3308. Hsu, Y. L., Kuo, P. L., Cho, C. Y., Ni, W. C., Tzeng, T. F., Ng, L. T., Kuo, Y. H., and Lin. C. C. 2007. Antrodia cinnamomea fruiting bodies extract suppresses the invasive potential of human liver cancer cell line PLC/PRF/5 through inhibition of nuclear factor kappaB pathway. Food Chem. Toxicol. 45: 1249-57. Huang, L. C., Huang, S. J., Chen, C. C., and Mau, J. L. 1999. Antioxidant properties of Antrodia camphorata. Proc. Third Int. Conf. Mush. Biol. Mush. Prod. 275-283. Huang, S. J., Tsai, S. Y., and Mau, J. L. 2006. Antioxidant properties of methanolic extracts from Agrocybe cylindracea. LWT -Food Sci. Technol. 39: 378-386. Huang, S., and Mau, J. 2007. Antioxidant properties of methanolic extracts from Antrodia camphorata with various doses of gamma-irradiation. Food Chem. 105: 1702–1710. Hwang, C. S., Rhie, G. E., Oh, J. H., Huh, W. K., Yim, H. S., and Kang, S. O. 2002. Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is required for the protection of Candida albicans against oxidative stresses and the expression of its full virulence. Microbiology 148: 3705-3713. Inoue, M., Sato, E. F., Nishikawa, M., Hiramoto, K., Kashiwagi, A., and Utsumi, K. 2004. Free radical theory of apoptosis and metamorphosis. Redox Rep. 9: 237-247. Intes, L., Bahut, M., Nicole, P., Couvineau, A., Guette, C., and Calenda, A. 2012. Intestinal cell targeting of a stable recombinant Cu–Zn SOD from Cucumis melo fused to a gliadin peptide. J. Biotech. 159: 99-107. James, F. K., and Donna, L. H. 1988. Formation of recombinant protein inclusion bodies in Escherichia coli. Trends Biotechnol. 6: 95-101. Jiang, H., and English, A. M. 2006. Phenotypic analysis of the ccp1Δ and ccp1Δ-ccp1W191F mutant strains of Saccharomyces cerevisiae indicates that cytochrome c peroxidase functions in oxidative-stress signaling. J. Inorg. Biochem. 100: 1996-2008. Kaput, J., Goltz, S., and Blobel, G. 1982. Nucleotide sequence of the yeast nuclear gene for cytochrome c peroxidase precursor: implications of the presequence for protein transport into mitochondria. J. Biol. Chem. 257: 15054-15058. Kendall, B. W. 1997. Free radical toxicology. CRC Press 1-352. Kojima, T., Wakamatsu, T. H., Dogru, M., Ogawa, Y., Igarashi, A., Ibrahim, O. M., Inaba, T., Shimizu, T., Noda, S., Obata, H., Nakamura, S., Wakamatsu, A., Shirasawa, T., Shimazaki, J., Negishi, K., and Tsubota, K. 2012. Age-related dysfunction of the lacrimal gland and oxidative stress: evidence from the Cu, Zn-superoxide dismutase-1 (Sod1) knockout mice. Am. J. Pathol. 180: 1879-96. Lee, E. J., and Jang, H. D. 2004. Antioxidant activity and protective effect of five edible mushrooms on oxidative DNA damage. Food Sci. Biotechnol. 13: 443-449. Lee, I. H., Huang, R. L., Chen, C. T., Chen, H. C., Hsu, W. C., and Lu M. K. 2002. Antrodia camphorate polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiol. Lett. 209: 63-67. Lee, J., Godon, C., Lagniel, G., Spector, D., Garin, J., Labarre, J., and Toledano, M. B. 1999. Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. J. Biol. Chem. 274: 16040-16046. Lee, Y. L., Huang, G. H., Liang, Z. C., and Mau, J. L. 2007. Antioxidant properties of three extracts from Pleurotus citrinopileatus. LWT -Food Sci. Technol. 40: 823-833. Leonowicz, A., Matuszewska, A., Luterek, J., Ziegenhagen, D., Wasilewska, W. M., Cho, N. S., Hofrichter, M., and Rogalski, J. 1999. Biodegradation of lignin by white rot fungi. Fungal Genet. Biol. 27: 175-185. Liau, Y.J., L. Wen, J.F. Shaw, and Lin, C.T. 2007. A highly stable cambialistic-superoxide dismutase from Antrodia camphorata: expression in yeast and enzyme properties. J. Biotech. 131: 84-91. Lin, M. T., Kuo, T. J., and Lin, C. T. 1998. Molecular cloning of a cDNA encoding copper/zinc superoxide dismutase from papaya fruit and overexpression in Escherichia coli. J. Agric. Food Chem. 46: 344-348. Lin, W. C., Kuo, S. C., Lin, W. L., Fang, H. L., and Wang, B. C. 2006. Filtrate of fermented mycelia from Antrodia camphorate reduces liver fibrosis induced by carbon tetrachloride in rats. World J. Gastroenterol. 12: 2369-2374. Liu, D., Liang, H., Chen, C., Su, C., Lee, T., Huang, C., Hou, W., Lin, S., Zhong, W., Lin, P., Hung, L., and Liang, Y. 2007. Comparative anti-inflammatory characterization of wild fruiting body, liquid-state fermentation, and solid-state culture of Taiwanofungus camphoratus in microglia and the mechanism of its action. J. Ethnopharmacol. 113: 5-53. Liu, D. Z., Liang, Y. C., Lin, S. Y., Lin, Y. S., Wu, W. C., Hou, W. C., and Su, C. H. 2007. Antihypertensive activities of a solid-state culture of Taiwanofungus camphoratus (Chang-Chih) in spontaneously hypertensive rats. Biosci. Biotechnol Biochem. 71: 23-30. Maccecchini, M. L., Rudin, Y., and Schatz, G. 1979. Transport of proteins across the mitochondrial outer membrane: a precursor form of the cytoplasmically made intermembrane enzyme cytochrome c peroxidase. J. Biol. Chem. 254: 7468-7471. Mann, T., and Keilin, D. 1939. Haemocuprin and hepatocuprin, copper-proein compounds of blood and liver in mammals. Proc. R. Soc Lond. [Biol.] 128: 303-304. Mau, J. L., Lin, H. C., and Song, S. F. 2002. Antioxidant properties of several specialty mushrooms. Food Res. Int. 35: 519-526. Mau, J. L., Chang, C. N., Huang, S. J., and Chen, C. C. 2004. Antioxidant properties of methanolic extracts from Grifola frondosa, Morchella esculenta and Termitomyces albuminosus mycelia. Food Chem. 87: 111-118. Mau, J. L., Huang, P. N., Huang, S. J., and Chen .C. C. 2004. Antioxidant properties of methanolic extracts from two kinds of Antrodia camphorata mycelia. Food Chem. 86: 25-31. McCord, J. M., and Edeas, M. A. 2005. SOD, oxidative stress and human pathologies: a brief history and a future vision. Biomed. Pharmacother. 59: 139-142. Mei, H., Wang, K., Peffer, N., Weatherly, G., Cohen, D. S., Miller, M., Pielak, G., Durham, B., and Millett, F. 1999. Role of configurational gating in intracomplex electron transfer from cytochrome c to the radical cation in cytochrome c peroxidase. Biochemistry 38: 6846-6854. McCord, J. M., and Fridovich, I. 1969. Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244: 6049-6052. McDongh, O. B., Gralla, E. B., and Valentine, J. S. 1988. The copper, zinc-superoxide dismutase gene of Saccharomyces cerevisiae: cloning, sequencing, and biological activity. Proc. Natl. Acad. Sci. U.S.A. 85: 4789-4793. Miller, V. P., DePillis, G. D., Ferrer, J. C., Mauk, A. G., and Ortiz de Montellano, P. R. 1992. Monooxygenase activity of cytochrome c peroxidase. J. Biol. Chem. 267: 8936-42. Nakamura, N., Hirakawa, A., Gao, J. J., Kakuda, H., Shiro, M., Komatsu, Y., Sheu, C. C., and Hattori, M. 2004. Five new maleic and succinic acid derivatives from the mycelium of Antrodia camphorata and their cytotoxic effects on LLC tumor cell line. J. Nat. Prod. 67: 46-48. Nakani, S., Vitello, L. B., and Erman, J. E. 2006a. Characterization of four covalently-linked yeast cytochrome c/cytochrome c peroxidase complexes: evidence for electrostatic interaction between bound cytochrome c molecules. Biochemistry 45: 14371-14378. Nakani, S., Viriyakul, T., Mitchell, R., Vitello, L. B., and Erman, J. E. 2006b. Charaterization of a covalently linked yeast cytochrome c-cytochrome c peroxidase complex: evidence for a single, catalytically active cytochrome c binding site on cytochrome c peroxidase. Biochemistry 45: 9887-9893. Narasipura, S. D., Ault, J. G., Behr, M. J., Chaturvedi, V., and Chaturvedi, S. 2003. Characterization of Cu, Zn superoxide dismutase (SOD1) gene knock-out mutant of Cryptococcus neoformans var. gattii: role in biology and virulence. Mol. Microbiol. 47: 1681-1694. Pan, S. M., Hwang, G. B., and Liu, H. C. 1999. Overexpression of rice CuZnSOD in E. coli. Bot. Bull. Acad. Sin. 40: 275-281. Park, N. S., Lee, R. S., Sohn, H. D., and Kim, D. H. 2005. Molecular cloning, expression, and characterization of the Cu, Zn superoxide dismutase (SOD1) gene from the entomopathogenic fungus Cordyceps militaris. Mycologia 97: 130-138. Passardi, F., Bakalovic, N., Teixeira, F. K., Margis-Pinheiro, M., Penel, C., and Dunand, C. 2007. Prokaryotic origins of the non-animal peroxidase superfamily and organelle-mediated transmission to eukaryotes. Genomics 89: 567–579. Passardi, F., Theiler, G., Zamocky, M., Cosio, C., Rouhier, N., Teixera, F., Margis-Pinheiro, M., Ioannidis, V., Penel, C., Falquet, L., and Dunand, C. 2007. PeroxiBase: The peroxidase database. Phytochemistry. 68: 1605-11. Peng, C. C., Chen, K. C., Peng, R. Y., Su, C. H., and Hsieh-Li, H. M. 2006. Human urinary bladder cancer T24 cells are susceptible to the Antrodia camphorata extracts. Cancer Lett. 243: 109-119. Pfister, T. D., Gengenbach, A. J., Syn, S., and Lu, Y. 2001. The role of redox-active amino acids on compound I stability, substrate oxidation, and protein cross-linking in yeast cytochrome c peroxidase. Biochemistry 40: 14942-14951. Planner, N., Hartl, F. U., and Neupert, W. 1988. Import of proteins into mitochondria: a multi-step process. Eur. J. Biochem. 175: 205-212. Poulos, T. L., and Kraut, J. 1980. A hypothetical model of eht cytochrome c peroxidase ․cytochrome c electron transfer complex. J. Biol. Chem. 255: 10322-10330. Poulos, T. L., Freer, S. T., Xuong, A. N., Edwards, S. L., Hamlin, R. C., and Kraut, J. 1978. Crystallographic determination of the heme orientation and location of the cyanide binding site in yeast cytochrome c peroxidase. J. Biol. Chem. 253: 3730-3735. Rao, Y., Fang, S., and Tzeng, Y. 2007. Evaluation of the anti-inflammatory and anti-proliferation tumoral cells activities of Antrodia camphorata, Cordyceps sinensis, and Cinnamomum osmophloeum bark extracts. J. Ethnopharmacol. 114: 78-85. Sakamoto, A., Ohsuga, H., and Tanaka, K. 1992. Nucleotide sequences of two cDNA clones encoding different Cu/Zn-superoxide dismutase expressed in developing rice seed (Oryza sativa L.). Plant Mol. Biol. 19: 323-327. Savoie, J. M., Minvielle, N., and Largeteau, M. 2008. Radical scavenging properties of extracts from the white button mushroom Agaricus bisporus. J. Agric. Food Chem. 88: 970-975. Schatz, G. 1987. Signals guiding proteins to their correct locations in mitochondria. Eur. J. Biochem. 165: 1-6. Shen, Y. C., Chou, C. J., Wang, Y. H., Chen, C. F., Chou, Y. C., and Lu, M. K. 2004. Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with watersoluble fractions from five different Cinnamomum species. FEMS Microbiol. Lett. 231: 137-143. Shiao, M. S., and Lin, L. J. 1987. Two new triterpenes of the fungus Ganoderma lucidum. J. Nat. Prod. 50: 886-890. Siddique, T., Figlewicz, D. A., Pericak-Vance, M. A., Haines, J. L., Rouleau, G., Jeffers, A. J., Sapp, P., Hung, W. Y., Bebout, J., McKenna-Yasek, D., Deng, G., Horvitz, H. R., Gusella, J. F., Brown, R. H., and Roses, A. D. 1991. Linkage of a gene causing familial amyotrophic lateral sclerosis to chromosome 21 and evidence of genetic-locus heterogeneity. N. Engl. J. Med. 324: 1381–1384. Sies, H. 1993. Strategies of antioxidant defense. Eur. J. Biochem. 215: 213-219. Sigman, J. A., Pond, A. E., Dawson, J. H., and Lu, Y. 1999. Engineering cytochrome c peroxidase into cytochrome P450: A proximal effect on heme-thiolate ligation. Biochemistry 38: 11122-11129. Silar, P. 2012. Hyphal interference: self versus non-self fungal recognition and hyphal death. Biocommunication of Fungi Günther Witzany ed. pp. 155-170. Song, T. Y., and Yen, G. C. 2002. Antioxidant properties of Antrodia camphorata in submerged culture. J. Agric. Food Chem. 50: 3322-3327. Song, T. Y., and Yen, G. C. 2003. Protective effects of fermented filtrate from Antrodia camphorata in submerged culture against CCl4-induced hepatic toxicity in rats. J. Agric. Food Chem. 51: 1571-1577. Song, T. Y., Hsu, S. L., Yeh, C. T., and Yen, G. C. 2005. Mycelia from Antrodia camphorata in submerged culture induce apoptosis of human hepatoma HepG2 cells possibly through regulation of Fas pathway. J. Agric. Food Chem. 53: 5550-5564. Southorn, P. A., and Powis, G. 1988a. Free radicals in medicine. I. Chemical nature and biologic reactions. Mayo Clin. Proc. 63: 381-389. Southorn, P. A., and Powis, G. 1988b. Free radicals in medicine. II. Involvement in human disease. Mayo Clin. Proc. 63: 390-408. Sturtz, L. A., Diekert, K., Jensen, L. T., Lill, R., and Culotta, V. C. 2001. A fraction of yeast Cu,Zn-superoxide dismutase and its metallochaperone, ccs, localize to the intermembrane space of mitochondria. a physiological role for sod1 in guarding against mitochondrial oxidative damage. J. Biol. Chem. 276: 38084-38089. Swartz, J. R. 2001. Advances in Escherichia coli production of therapeutic proteins. Curr. Opin. Biotechnol. 12: 195-201. Tsaprailis, G., and English, A. M. 2003. Different pathways of radical translocation in yeast cytochrome c peroxidase and its W191F mutant on reaction with H2O2 suggest an antioxidant role. J. Biol. Inorg. Chem. 8: 248-255. Turrens, J. F. 2003. Mitochondrial formation of reactive oxygen species. J. Physiol. 552: 335–344 van Loon, A. P., Brandli, A. W., and Schatz, G. 1986. The presequence of two imported mitochondrial proteins contain information for intracellular and intramitochondrial sorting. Cell 44: 801-812. Verner, K., and Schatz, G. 1988. Protein translocation across membranes. Science 241: 1307-1313. Volkov, A. N., Worrall, J. A., Holtzmann, E., and Ubbink, M. 2006. Solution structure and dynamics of the complex between cytochrome c and cytochrome c peroxidase determined by paramagnetic NMR. Proc. Natl. Acad. Sci. U.S.A. 103: 18945-18950. Waldmeyer, B., Bechtold, R., Bosshard, H. R., and Poulos, T. L. 1982. The cytochrome c peroxidase․cytochrome c electron transfer complex- Experimental support of hypothetical model. J. Biol. Chem. 257: 6073-6076. Wang, Z. S., He, Z. J., Shen, Q., Gu, Y. X., Li, S. X., and Yuan, Q. S. 2005. Purification and partial characterization of recombinant Cu, Zn containing superoxide dismutase of Cordyceps militaris in E. coli. J. Chromatogr. B 826: 114-121. Wen, L., Huang, H. M., Juang, R. H., and Lin, C. T. 2007. Biochemical characterization of 1-Cys peroxiredoxin from Antrodia camphorata. Appl. Microbiol. Biotechnol. 73: 1314-1322. Williams, C. H. 1976. In “The Enzymes” (P. D. Boyer, ed). Acad. Press 13: 89-132. Wu, S. H., Yu, Z. H., Dai, Y. C., Chen, C. T., Su, C. H., Chen, L. C., Hsu, W. C., and Hwang, G. Y. 2004. Taiwanofungus, a polypore new genus. Fung. Sci. 19: 109-116. Wu, S. H., Ryvarden, L., and Chang, T. T. 1997. Antrodia camphoratum (“niu-chang-chih”), new combination of a medicinal fungus in Taiwan. Bot. Bull. Acad. Sin. 38: 273-275. Yamada, K., Nakagawa, C. W., and Mutoh, N. 1999. Schizosaccharomyces pombe homologue of glutathione peroxidase, which does not contain selenocysteine, is induced by several stresses and works as an antioxidant. Yeast 15: 1125-1132. Yang, H. L., Chen, C. S., Chang, W. H., Lu, F. J., Lai, Y. C., Chen, C. C., Hseu, T. H., Kuo, C. T., and Hseu, Y. C. 2006. Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. Cancer Lett. 231: 215-227. Yeung, B. K., Wang, X., Sigman, J. A., Petillo, P. A., and Lu, Y. 1997. Construction and characterization of a manganese-binding site in cytochrome c peroxidase: towards a novel manganese peroxidase. Chem. Biol. 4: 215-221. Yonetani, T., and Schleyer, H. 1967. Studies on cytochrome c peroxidase IX. The reaction of ferrimyoglobin with hydroperoxides and a comparison of peroxide-induced compounds of ferrimyoglobin and cytochrome c peroxidase. J. Biol. Chem. 242: 1974-1979. Yu, Z. H., Wu, S. H., Wang, D. M., and Chen, C. T. 2010. Phylogenetic relationships of Antrodia species and related taxa based on analyses of nuclear large subunit ribosomal DNA sequences. Bot. Stud. 51: 53-60. Zamocky, M., and Dunand, C. 2006. Divergent evolutionary lines of fungal cytochrome c peroxidases belonging to the superfamily of bacterial, fungal and plant heme peroxidases. FEBS Lett. 580: 6655-6664. Zang, H., He, S., and Mauk, A. G. 2002. Radical formation at Tyr39 and Tyr153 following reaction of yeast cytochrome c peroxidase with hydrogen peroxide. Biochemistry 41: 13507-13513. Zang, M., and Su, C. H. 1990. Ganoderma comphoratum, a new taxon in genus Ganoderma from Taiwan. Acta Bot. Yunnanica 12: 393-396. Zelko, I. N., Mariani, T. J., and Folz, R. J. 2002. Superoxide dismutase multigene family: a comparison of the CuZn-SOD(SOD1), Mn-SOD(SOD2), and EC-SOD(SOD3) gene structures, evolution, and expression. Free Radic. Biol. Med. 33: 337-349. Zemlyak, I., Nimon, V., Brooke, S., Moore, T., McLaughlin, J., and Sapolsky, R. 2006. Gene therapy in the nervous system with superoxide dismutase. Brain Res. 1088: 12-18. Zimmermann, R., Flohe, L., Weser, U., and Hartmann, H. J. 1973. Inhibition of lipid peroxidation in isolated inner membrane of rat liver mitochondria by superoxide dismutase. FEBS Lett. 29: 117-120. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57623 | - |
dc.description.abstract | 牛樟芝(Antrodia cinnamomea) 為生長於台灣特有種之牛樟樹(Cinnamonum kanehirae Hayata) 樹心空胴壁處之珍貴且特別的醫藥菇蕈。牛樟芝具有多種有益生理活性,其中包括強抗氧化成份而使其具備良好抗發炎效果。抗氧化與抗發炎可有效抑制活性氧物種(reactive oxygen species, ROS),而牛樟芝抗氧化成份中含不少抗氧化酶,其細胞色素c過氧化酶(cytochrome c peroxidase, CcP)、銅鋅型超氧歧化酶(superoxide dismutase, Cu/Zn SOD) 均具清除自由基之抗氧化效果。超氧歧化酶具催化超氧自由基(超氧陰離子) 成過氧化氫和氧分子的能力,目前超氧歧化酶因其活性位所結合的金屬不同可分為四種類型:銅鋅型、錳型、鐵型、鎳型。細胞色素c過氧化酶則具催化過氧化氫成水分子的能力。由分析先前已建構完成之牛樟芝(Antrodia cinnamomea) cDNA library,推測可能為細胞色素c過氧化酶、超氧歧化酶者,將兩者 cDNAs 上傳於 NCBI GenBank 與 Swiss-Prot database 上比對鑑定確實符合該酵素特性,且歸屬於可知該超氧歧化酶類型應為銅鋅型;同時收集分析相近物種之酵素胺基酸序列,以找出保守性胺基酸位置。接著,再由多方面生物資訊分析該基因與胺基酸特性,以預測該蛋白質結構,以及是否具 signal peptide、transmembrane domain、醣基化等資訊,作為異源表現兩種酵素的依據;同時,對牛樟芝之細胞色素c過氧化酶與銅鋅型超氧歧化酶進行類緣分析,結合所得之生物資訊學的資料,從中發現有關這兩個酵素在演化上可能代表的意義,並進而解釋牛樟芝之序列獨特性。為更近一步探究牛樟芝細胞色素c過氧化酶和銅鋅型超氧歧化酶,我們將牛樟芝 gDNA 與 cDNA 全長已解序之兩種抗氧化酶開放讀碼區(open reading frame, ORF) 建構於適合表現的載體上,並將其轉入大腸桿菌(Escherichia coli) 系統中,誘導重組蛋白之表現,再以 Ni-NTA 親和性管柱純化出該重組蛋白。牛樟芝細胞色素過氧化酶在大腸桿菌系統中有較好重組蛋白之表現,對其進行酵素活性測試測得該酵素在酸鹼值 pH 7與 pH 5,溫度42 ℃有最佳的活性;在最佳條件下進行酵素動力學之分析,得到 Vmax=1.06×10-5 M/min 及 Km=0.161 M。牛樟芝細胞色素c過氧化酶具有清除過氧化氫的能力,這樣的現象也同樣可印證在正常倉鼠卵巢細胞(chinese hamster ovary cell, CHO cell) 模式上,清除細胞外添加之過氧化氫有助於避免細胞內產生過量之活性氧物種,達到保護細胞免受胞內過量活性氧物種之傷害。牛樟芝細胞色素c過氧化酶被設計以褐藻膠包覆進行微膠囊化,以期待該酵素能有儲藏之穩定性。然而,微膠囊化在製備的過程卻會導致該酵素活性幾乎喪失,且儲藏之穩定性僅能維持兩周,整體來說,該酵素若要進一步進行微膠囊化之實際應用,改善過程中大量活性喪失的情況是最主要的關鍵所在,故酵素方面的微膠囊化方面仍有待研究探討。 | zh_TW |
dc.description.abstract | Antrodia cinnamomea is an unique precious medicinal mushroom, inhabiting on the inner cavity wall of Cinnamonum kanehirae Hayata, an endemic species of Taiwan. Many beneficial biological activity of Antrodia cinnamomea have been reported. One of them is anti-oxidation and anti-inflammation against the reactive oxygen species (ROS). Cytochrome c peroxidase (CcP) and Cu/Zn superoxide dismutase (Cu/Zn SOD) both are anti-oxidative enzymes. Superoxide dismutase is capable of catalyzing the dismutation of the superoxide ion (O2•−) to hydrogen peroxide and molecular oxygen. Based on the affinity of metal elements which bind to the active sites, superoxide dismutase can be classified into four types:Cu/Zn, Mn, Fe, and Ni. Cytochrome c peroxidase is a mitochondrial enzyme that catalyzes the reduction of hydrogen peroxide to water. The putative SOD and CcP genes were accessed in our previously constructed Antrodia cinnamomea cDNA library, and were demonstrated homologous to Cu/Zn SOD and CcP while blasting NCBI GenBank and Swiss-Prot database. The amino acid sequences were used to acquire the bioinformatics from bioinformatics resource portal, in order to figure out the characterizations of the two anti-oxidative enzymes and find the conditions of heterologous protein expression. Meanwhile, the phylogeny analysis of cytochrome c peroxidase and Cu/Zn superoxide dismutase show the significance of species evolution and can be used to explain the unique characteristics of Antrodia cinnamomea by the bioinformatics of amino acid sequences. The putative AC-Cu/Zn SOD and AC-CcP were cloned, characterized, and their gDNA and cDNA full-length was worked out. The open reading frame of AC-Cu/Zn SOD and AC-CcP were constructed into E. coli expression vector. The recombinant protein was purified to homogeneity by Ni-NTA affinity chromatography. The recombinant protein of AC-CcP was well expressed in E. coli expression system. In enzyme acivity test, AC-CcP had the best activity at pH 7, pH 5 and best reaction temperature at 42 ℃. In the best reaction condition, the result of the enzymatic kinetics got AC-CcP Vmax=1.06×10-5 M/min and Km=0.161 M. AC-CcP had ability to eliminate hydrogen peroxide. It also can find that AC-CcP prevent ROS in CHO cell (chinese hamster ovary cell) from overproducting. Therefore, AC-CcP should had the ability to protect cell from damege that induced by hydrogen peroxide. The microencapsulation of AC-CcP were coated with alginate and hope to provide the enzyme with stability of storage. However, the process led to loss the activity almost. The storage stability of the microcapsulated AC-CcP was just 2 weeks. In the case of microencapsulating enzyme, how to enhance activity of enzyme was an importance issue to be investigated. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T06:54:38Z (GMT). No. of bitstreams: 1 ntu-103-R00633011-1.pdf: 9127238 bytes, checksum: f5b930b933a91e1075a4673d9a5e1779 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 iii Abstract v 目錄 vii 表目錄 x 圖目錄 xi 縮寫對照表 xiv 壹、 前言 1 貳、 前人研究 4 I. 牛樟芝簡介 4 一、 分類地位 4 二、 型態特徵 4 三、 成分分析 5 四、 生物功能活性研究 7 II. 自由基與抗氧化機制 12 一、 自由基的產生 12 二、 自由基的危害 12 三、 活性氧物種 (reactive oxygen species, ROS) 13 四、 生物抗氧化機制 13 五、 抗氧化能力影響其他生物功能活性 14 III. 牛樟芝抗氧化酵素 16 一、 細胞色素c過氧化酶 (Cytochrome c peroxidase, CcP) 18 二、 銅鋅型超氧歧化酶 (Cu/Zn superoxide dismutase, Cu/Zn SOD) 22 三、 牛樟芝細胞色素c過氧化酶與牛樟芝銅鋅型超氧歧化酶之研究動機 25 參、 材料與方法 27 一、 菌株與培養基 27 二、 Genomic DNA 與 RNA 的萃取 27 三、 cDNA 的合成 29 四、 定序與建構 29 五、 目標蛋白質的表現與純化 33 六、 酵素活性分析 34 七、 穩定性測試 35 八、 酵素動力學 35 九、 序列分析 36 十、 細胞模式測試 37 十一、 微膠囊化 39 十二、 類源分析 40 肆、 結果 41 一、 序列分析 41 二、 類緣分析 47 三、 定序與建構 49 四、 目標蛋白質的表現 50 五、 酵素活性分析 51 六、 穩定性測試 52 七、 酵素動力學 53 八、 細胞模式測試 55 九、 微膠囊化 57 伍、 討論 58 參考文獻 68 附錄 137 | |
dc.language.iso | zh-TW | |
dc.title | 牛樟芝抗氧化酵素—細胞色素c過氧化酶與銅鋅型超氧歧化酶之分子選殖、蛋白質表現及功能性分析 | zh_TW |
dc.title | Molecular cloning, characterization, expression and functional analysis of anti-oxidative cytochrome c peroxidase and copper/zinc superoxide dismutase from Antrodia cinnamomea | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉瑞芬(Ruey-Fen Liou),陳明汝(Ming-Ju Chen),沈湯龍(Tang-Long Shen),林乃君(Nai-Chun Lin) | |
dc.subject.keyword | 牛樟芝,牛樟樹,抗氧化?,細胞色素c 過氧化?,銅鋅型超氧歧化?,類緣分析,異源蛋白質表現,細胞模式測式,微膠囊化, | zh_TW |
dc.subject.keyword | Antrodia cinnamomea,Cinnamonum kanehirae Hayata,antioxidative enzymes,cytochrome c peroxidase,Cu/Zn superoxide dismutase,phylogeny,heterologous protein expression,cell model test,microencapsulation, | en |
dc.relation.page | 149 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-07-21 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 目前未授權公開取用 | 8.91 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。