請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57609
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 牟中原(Chung-Yuan Mou) | |
dc.contributor.author | Chieh-Jui Tsou | en |
dc.contributor.author | 鄒劼叡 | zh_TW |
dc.date.accessioned | 2021-06-16T06:53:55Z | - |
dc.date.available | 2014-08-08 | |
dc.date.copyright | 2014-08-08 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-07-21 | |
dc.identifier.citation | 1. Kresge, C. T.; Leonowicz, M. E.; Roth, W. J.; Vartuli, J. C.; Beck, J. S. Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism. Nature 1992, 359, 710-712.
2. Nooney, R. I.; Thirunavukkarasu, D.; Chen, Y.; Josephs, R.; Ostafin, A. E. Synthesis of Nanoscale Mesoporous Silica Spheres with Controlled Particle Size. Chem. Mater. 2002, 14, 4721-4728. 3. Lin, H.-P.; Mou, C.-Y. Structural and Morphological Control of Cationic Surfactant-Templated Mesoporous Silica. Acc. Chem. Res. 2002, 35, 927-935. 4. Zhao, D.; Huo, Q.; Feng, J.; Chmelka, B. F.; Stucky, G. D. Nonionic Triblock and Star Diblock Copolymer and Oligomeric Surfactant Syntheses of Highly Ordered, Hydrothermally Stable, Mesoporous Silica Structures. J. Am. Chem. Soc. 1998, 120, 6024-6036. 5. He, Q.; Zhang, Z.; Gao, Y.; Shi, J.; Li, Y. Intracellular Localization and Cytotoxicity of Spherical Mesoporous Silica Nano- and Microparticles. Small 2009, 5, 2722-2729. 6. Hoffmann, F.; Cornelius, M.; Morell, J.; Froba, M. Silica-Based Mesoporous Organic–Inorganic Hybrid Materials. Angew. Chem. Int. Ed. 2006, 45, 3216-3251. 7. Stober, W.; Fink, A.; Bohn, E. Controlled growth of monodisperse silica spheres in the micron size range. J. Colloid Interface Sci. 1968, 26, 62-69. 8. Grun, M.; Lauer, I.; Unger, K. K. The synthesis of micrometer- and submicrometer-size spheres of ordered mesoporous oxide MCM-41. Adv. Mater. 1997, 9, 254-257. 9. Wu, S. H.; Hung, Y.; Mou, C. Y. Mesoporous silica nanoparticles as nanocarriers. Chem.commun.2011, 47, 9972-85. 10. Lu, F.; Wu, S.-H.; Hung, Y.; Mou, C.-Y. Size Effect on Cell Uptake in Well-Suspended, Uniform Mesoporous Silica Nanoparticles. Small 2009, 5, 1408-1413. 11. Wang, Y.; Caruso, F. Enzyme encapsulation in nanoporous silica spheres. Chem. Commun. 2004, 0, 1528-1529. 12. Liu, R.; Liao, P.; Liu, J.; Feng, P. Responsive Polymer-Coated Mesoporous Silica as a pH-Sensitive Nanocarrier for Controlled Release. Langmuir 2011, 27(6), 3095-3099. 13. Lin, Y.-S.; Tsai, C.-P.; Huang, H.-Y.; Kuo, C.-T.; Hung, Y.; Huang, D.-M.; Chen, Y.-C.; Mou, C.-Y. Well-Ordered Mesoporous Silica Nanoparticles as Cell Markers. Chem. Mater. 2005, 17, 4570-4573. 14. Lee, C.-H.; Cheng, S.-H.; Wang, Y.-J.; Chen, Y.-C.; Chen, N.-T.; Souris, J.; Chen, C.-T.; Mou, C.-Y.; Yang, C.-S.; Lo, L.-W. Near-Infrared Mesoporous Silica Nanoparticles for Optical Imaging: Characterization and In Vivo Biodistribution. Adv. Funct. Mater. 2009, 19, 215-222. 15. Lei, J.; Wang, L.; Zhang, J. Ratiometric pH sensor based on mesoporous silica nanoparticles and Forster resonance energy transfer. Chem. Commun. 2010, 46, 8445-7. 16. Vivero-Escoto, J. L.; Huxford-Phillips, R. C.; Lin, W. Silica-based nanoprobes for biomedical imaging and theranostic applications. Chem. Soc. Rev. 2012, 41, 2673-2685. 17. Yang, P.; Gai, S.; Lin, J. Functionalized mesoporous silica materials for controlled drug delivery. Chem. Soc. Rev. 2012, 41, 3679-3698. 18. Liu, R.; Liao, P.; Liu, J.; Feng, P. Responsive Polymer-Coated Mesoporous Silica as a pH-Sensitive Nanocarrier for Controlled Release. Langmuir 2011, 27, 3095-3099. 19. Chen, Y.-P.; Chen, H.-A.; Hung, Y.; Chien, F.-C.; Chen, P.; Mou, C.-Y. Surface charge effect in intracellular localization of mesoporous silica nanoparticles as probed by fluorescent ratiometric pH imaging. RSC Adv.2012, 2, 968. 20. Li; Shi; Hua; Chen; Ruan; Yan. Hollow Spheres of Mesoporous Aluminosilicate with a Three-Dimensional Pore Network and Extraordinarily High Hydrothermal Stability. Nano Lett. 2003, 3, 609-612. 21. Tan, B.; Rankin, S. E. Dual Latex/Surfactant Templating of Hollow Spherical Silica Particles with Ordered Mesoporous Shells. Langmuir 2005, 21, 8180-8187. 22. Yang, Y.; Liu, J.; Li, X.; Liu, X.; Yang, Q. Organosilane-Assisted Transformation from Core–Shell to Yolk–Shell Nanocomposites. Chem. Mater. 2011, 23, 3676-3684. 23. Okamoto, M.; Huang, H. Formation of hollow silica spheres with ordered mesoporous structure by treatment with dimethyl carbonate for selective decomposition of mesoporous silica core. Micropor. Mesopor. Mater. 2012, 163, 102-109. 24. Zhang, A.; Zhang, Y.; Xing, N.; Hou, K.; Guo, X. Hollow Silica Spheres with a Novel Mesoporous Shell Perforated Vertically by Hexagonally Arrayed Cylindrical Nanochannels. Chem. Mater. 2009, 21, 4122-4126. 25. Wang, Y.; Tang, C.; Deng, Q.; Liang, C.; Ng, D. H.; Kwong, F. L.; Wang, H.; Cai, W.; Zhang, L.; Wang, G. A versatile method for controlled synthesis of porous hollow spheres. Langmuir 2010, 26, 14830-14834. 26. Li, J.; Liu, J.; Wang, D.; Guo, R.; Li, X.; Qi, W. Interfacially controlled synthesis of hollow mesoporous silica spheres with radially oriented pore structures. Langmuir 2010, 26, 12267-1226772. 27. Wang, D. P.; Zeng, H. C. Creation of Interior Space, Architecture of Shell Structure, and Encapsulation of Functional Materials for Mesoporous SiO2Spheres. Chem. Mater. 2011, 23, 4886-4899. 28. Wu, S.-H.; Hung, Y.; Mou, C.-Y. Compartmentalized Hollow Silica Nanospheres Templated from Nanoemulsions. Chem. Mater. 2013, 25, 352-364. 29. Blas, H. l. n.; Save, M.; Pasetto, P.; Boissière, C. d.; Sanchez, C. m.; Charleux, B. Elaboration of Monodisperse Spherical Hollow Particles with Ordered Mesoporous Silica Shells via Dual Latex/Surfactant Templating: Radial Orientation of Mesopore Channels. Langmuir 2008, 24, 13132-13137. 30. Schacht, S.; Huo, Q.; Voigt-Martin, I. G.; Stucky, G. D.; Schuth, F. Oil-Water Interface Templating of Mesoporous Macroscale Structures. Science 1996, 273, 768-771. 31. Lin, Y.-S.; Wu, S.-H.; Tseng, C.-T.; Hung, Y.; Chang, C.; Mou, C.-Y. Synthesis of hollow silica nanospheres with a microemulsion as the template. Chem. Commun. 2009, 24, 3542-3544. 32. Tanev, P. T.; Pinnavaia, T. J. Biomimetic Templating of Porous Lamellar Silicas by Vesicular Surfactant Assemblies. Science 1996, 271, 1267-1269. 33. Tanev, P. T.; Liang, Y.; Pinnavaia, T. J. Assembly of Mesoporous Lamellar Silicas with Hierarchical Particle Architectures. J. Am. Chem. Soc. 1997, 119, 8616-8624. 34. Huang, X.; Meng, X.; Tang, F.; Li, L.; Chen, D.; Liu, H.; Zhang, Y.; Ren, J. Mesoporous magnetic hollow nanoparticles—protein carriers for lysosome escaping and cytosolic delivery. Nanotech. 2008, 19, 445101. 35. Yang, J.; Lee, J.; Kang, J.; Lee, K.; Suh, J.-S.; Yoon, H.-G.; Huh, Y.-M.; Haam, S. Hollow Silica Nanocontainers as Drug Delivery Vehicles. Langmuir 2008, 24, 3417-3421. 36. Lin, W.-I.; Lin, C.-Y.; Lin, Y.-S.; Wu, S.-H.; Huang, Y.-R.; Hung, Y.; Chang, C.; Mou, C.-Y. High payload Gd(iii) encapsulated in hollow silica nanospheres for high resolution magnetic resonance imaging. J. Mater. Chem. B 2013, 1, 639-645. 37. Wu, S.-H.; Tseng, C.-T.; Lin, Y.-S.; Lin, C.-H.; Hung, Y.; Mou, C.-Y. Catalytic nano-rattle of Au@hollow silica: towards a poison-resistant nanocatalyst. J. Mater. Chem. 2011, 21, 789-794. 38. Das, S. K.; Bhunia, M. K.; Chakraborty, D.; Khuda-Bukhsh, A. R.; Bhaumik, A. Hollow spherical mesoporous phosphosilicate nanoparticles as a delivery vehicle for an antibiotic drug. Chem. Commun. 2012, 48, 2891-2893. 39. Tao, C.; JiaJun, F. pH-responsive nanovalves based on hollow mesoporous silica spheres for controlled release of corrosion inhibitor. Nanotech. 2012, 23, 235-605. 40. Kao, K.-C.; Tsou, C.-J.; Mou, C.-Y. Collapsed (kippah) hollow silica nanoparticles. Chem. Commun. 2012, 48, 3454-3456. 41 Vivero-Escoto, J. L.; Huxford-Phillips, R. C.; Lin, W. Chem. Soc. Rev. 2012, 41, 2673. 42 Yang, P.; Gai, S.; Lin, J. Chem. Soc. Rev. 2012, 41, 3679. 43 Ji, J.; Rosenzweig, N.; Griffin, C.; Rosenzweig, Z. Anal. Chem. 2000, 72, 3497. 44 Wu, S.-H.; Lin, Y.-S.; Hung, Y.; Chou, Y.-H.; Hsu, Y.-H.; Chang, C.; Mou, C.-Y. ChemBioChem 2008, 9, 53. 45 Lin, W.-I.; Lin, C.-Y.; Lin, Y.-S.; Wu, S.-H.; Huang, Y.-R.; Hung, Y.; Chang, C.; Mou, C.-Y. J.Mater. Chem. B 2013, 1, 639. 46 Kresge, C. T.; Leonowicz, M. E.; Roth, W. J.; Vartuli, J. C.; Beck, J. S. Nature 1992, 359, 710. 47 Lise, D.; Richter, C. P.; Drees, C.; Birkholz, O.; You, C.; Rampazzo, E.; Piehler, J. Nano Lett. 2014, 14, 2189. 48 Izumi, H.; Torigoe, T.; Ishiguchi, H.; Uramoto, H.; Yoshida, Y.; Tanabe, M.; Ise, T.; Murakami, T.; Yoshida, T.; Nomoto, M.; Kohno, K. Cancer treatment reviews 2003, 29, 541. 49 Davies, T. A.; Fine, R. E.; Johnson, R. J.; Levesque, C. A.; Rathbun, W. H.; Seetoo, K. F.; Smith, S. J.; Strohmeier, G.; Volicer, L.; Delva, L.; Simons, E. R. Biochem. Biophys. Res. Commun. 1993, 194, 537. 50 Burns, A.; Sengupta, P.; Zedayko, T.; Baird, B.; Wiesner, U. Small 2006, 2, 723. 51 Wang, L.; Lei, J.; Zhang, J. Chem. Commun. 2009, 2195. 52 Benjaminsen, R. V.; Sun, H.; Henriksen, J. R.; Christensen, N. M.; Almdal, K.; Andresen, T. L. ACS Nano 2011, 5, 5864. 53 Marin, M. J.; Galindo, F.; Thomas, P.; Russell, D. A. Angew. Chem. Int. Ed. 2012, 51, 9657. 54 Wang, X. D.; Stolwijk, J. A.; Lang, T.; Sperber, M.; Meier, R. J.; Wegener, J.; Wolfbeis, O. S. J. Am. Chem. Soc. 2012, 134, 17011. 55 Yin, L.; He, C.; Huang, C.; Zhu, W.; Wang, X.; Xu, Y.; Qian, X. Chem. Commun. 2012, 48, 4486. 56 Lei, J.; Wang, L.; Zhang, J. Chem. Commun. 2010, 46, 8445. 57 Chen, Y.-P.; Chen, H.-A.; Hung, Y.; Chien, F.-C.; Chen, P.; Mou, C.-Y. RSC Advances 2012, 2, 968. 58 Tsou, C.-J.; Chu, C.-Y.; Hung, Y.; Mou, C.-Y. J. Mater. Chem. B 2013, 1, 5557. 59 Wu, S. H.; Hung, Y.; Mou, C. Y. Chem. Commun. 2011, 47, 9972. 60 Lee, C.-H.; Lin, T.-S.; Mou, C.-Y. Nano Today 2009, 4, 165. 61 Dennis, A. M.; Rhee, W. J.; Sotto, D.; Dublin, S. N.; Bao, G. ACS Nano 2012, 6, 2917. 62 Doussineau, T.; Schulz, A.; Lapresta-Fernandez, A.; Moro, A.; Korsten, S.; Trupp, S.; Mohr, G. J. Chem. Eur. J. 2010, 16, 10290. 63 Peng, H. S.; Stolwijk, J. A.; Sun, L. N.; Wegener, J.; Wolfbeis, O. S. Angew. Chem. Int. Ed. 2010, 49, 4246. 64 Montiel, D.; Yang, H. Laser & Photonics Reviews 2010, 4, 374. 65 Saxton, M. J.; Jacobson, K. Annu. Rev. Biophys. Biomol. Struct. 1997, 26, 373. 66 Wells, N. P.; Lessard, G. A.; Goodwin, P. M.; Phipps, M. E.; Cutler, P. J.; Lidke, D. S.; Wilson, B. S.; Werner, J. H. Nano Lett. 2010, 10, 4732. 67 Reuel, N. F.; Dupont, A.; Thouvenin, O.; Lamb, D. C.; Strano, M. S. ACS Nano 2012, 6, 5420. 68 Welsher, K.; Yang, H. Nat Nano 2014, 9, 198. 69 McHale, K.; Berglund, A. J.; Mabuchi, H. Nano Lett. 2007, 7, 3535. 70 Juette, M. F.; Bewersdorf, J. Nano Lett. 2010, 10, 4657. 71 Han, J. J.; Kiss, C.; Bradbury, A. R. M.; Werner, J. H. ACS Nano 2012, 6, 8922. 72 Kao, H. P.; Verkman, A. S. Biophys. J. 1994, 67, 1291. 73 Holtzer, L.; Meckel, T.; Schmidt, T. Appl. Phys. Lett. 2007, 90. 74 Toprak, E.; Balci, H.; Blehm, B. H.; Selvin, P. R. Nano Lett. 2007, 7, 2043. 75 Huang, B.; Wang, W.; Bates, M.; Zhuang, X. Science 2008, 319, 810. 76 Li, Y.; Hu, Y.; Cang, H. J.Phys. Chem. B 2013, 117, 15503. 77 Stober, W.; Fink, A.; Bohn, E. J. Colloid Interface Sci. 1968, 26, 62. 78 Lin, H.-P.; Mou, C.-Y. Acc. Chem. Res. 2002, 35, 927. 79 Zhang, Y.; Ke, X.; Zheng, Z.; Zhang, C.; Zhang, Z.; Zhang, F.; Hu, Q.; He, Z.; Wang, H. ACS Nano 2013, 7, 3896. 80 Biermann, B.; Sokoll, S.; Klueva, J.; Missler, M.; Wiegert, J. S.; Sibarita, J. B.; Heine, M. Nat Commun 2014, 5, 1. 81. Roos, A.; Boron, W. F. Intracellular pH. Physiol. Rev. 1981, 61, 296-434. 82. Gottlieb, R. A.; Dosanjh, A. Mutant cystic fibrosis transmembrane conductance regulator inhibits acidification and apoptosis in C127 cells: possible relevance to cystic fibrosis. P. Natl. Acad. Sci. USA 1996, 93, 3587-3591. 83. Montrose, M.; Friedrich, T.; Murer, H. Measurements of intracellular pH in single LLC-PK1 cells: Recovery from an acid load via basolateral Na+/H+ exchange. J. Membrain Biol. 1987, 97, 63-78. 84. Schindler, M.; Grabski, S.; Hoff, E.; Simon, S. M. Defective pH Regulation of Acidic Compartments in Human Breast Cancer Cells (MCF-7) Is Normalized in Adriamycin-Resistant Cells (MCF-7adr)†. Biochem. 1996, 35, 2811-2817. 85. Ohkuma, S.; Poole, B. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. P. Natl. Acad. Sci. USA 1978, 75, 3327-3331. 86. Izumi, H.; Torigoe, T.; Ishiguchi, H.; Uramoto, H.; Yoshida, Y.; Tanabe, M.; Ise, T.; Murakami, T.; Yoshida, T.; Nomoto, M.; Kohno, K. Cellular pH regulators: potentially promising molecular targets for cancer chemotherapy. Cancer Treat. Rev. 2003, 29, 541-549. 87. Davies, T. A.; Fine, R. E.; Johnson, R. J.; Levesque, C. A.; Rathbun, W. H.; Seetoo, K. F.; Smith, S. J.; Strohmeier, G.; Volicer, L.; Delva, L.; Simons, E. R. Non-age Related Differences in Thrombin Responses by Platelets from Male Patients with Advanced Alzheimer′s Disease. Biochem. Biophys. Res. Commun. 1993, 194, 537-543. 88. Rink, T. J.; Tsien, R. Y.; Pozzan, T. Cytoplasmic pH and free Mg2+ in lymphocytes. J. Cell Bio. 1982, 95, 189-196. 89. Sun, W.-C.; Gee, K. R.; Klaubert, D. H.; Haugland, R. P. Synthesis of Fluorinated Fluoresceins. J. Org. Chem. 1997, 62, 6469-6475. 90. Kim, J. H.; Johannes, L.; Goud, B.; Antony, C.; Lingwood, C. A.; Daneman, R.; Grinstein, S. Noninvasive measurement of the pH of the endoplasmic reticulum at rest and during calcium release. P. Natl. Acad. Sci. USA 1998, 95, 2997-3002. 91. Jiao, G.-S.; Han, J. W.; Burgess, K. Syntheses of Regioisomerically Pure 5- or 6-Halogenated Fluoresceins. J. Org.c Chem. 2003, 68, 8264-8267. 92. Doussineau, T.; Schulz, A.; Lapresta-Fernandez, A.; Moro, A.; Korsten, S.; Trupp, S.; Mohr, G. J. On the Design of Fluorescent Ratiometric Nanosensors. Chem Eur. J. 2010, 16, 10290-10299. 93. Marin, M. J.; Galindo, F.; Thomas, P.; Russell, D. A. Localized Intracellular pH Measurement Using a Ratiometric Photoinduced Electron-Transfer-Based Nanosensor. Angew. Chem. Int. Ed. 2012, 51, 9657-9661. 94. Burns, A.; Sengupta, P.; Zedayko, T.; Baird, B.; Wiesner, U. Core/Shell fluorescent silica nanoparticles for chemical sensing: towards single-particle laboratories. Small 2006, 2, 723-6. 95. Jin, T.; Sasaki, A.; Kinjo, M.; Miyazaki, J. A quantum dot-based ratiometric pH sensor. Chem. Commun. 2010, 46, 2408-2410. 96. Schulz, A.; Wotschadlo, J.; Heinze, T.; Mohr, G. J. Fluorescent nanoparticles for ratiometric pH-monitoring in the neutral range. J. Mater. Chem. 2010, 20, 1475-1482. 97. Benjaminsen, R. V.; Sun, H.; Henriksen, J. R.; Christensen, N. M.; Almdal, K.; Andresen, T. L. Evaluating Nanoparticle Sensor Design for Intracellular pH Measurements. ACS Nano 2011, 5, 5864-5873. 98. Chan, Y.-H.; Wu, C.; Ye, F.; Jin, Y.; Smith, P. B.; Chiu, D. T. Development of Ultrabright Semiconducting Polymer Dots for Ratiometric pH Sensing. Anal. Chem. 2011, 83, 1448-1455. 99. Wang, X. D.; Stolwijk, J. A.; Lang, T.; Sperber, M.; Meier, R. J.; Wegener, J.; Wolfbeis, O. S. Ultra-small, highly stable, and sensitive dual nanosensors for imaging intracellular oxygen and pH in cytosol. J. Am. Chem. Soc. 2012, 134, 17011-4. 100. Peng, H. S.; Stolwijk, J. A.; Sun, L. N.; Wegener, J.; Wolfbeis, O. S. A nanogel for ratiometric fluorescent sensing of intracellular pH values. Angew. Chem. Int. Ed. 2010, 49, 4246-9. 101. Lei, J.; Wang, L.; Zhang, J. Ratiometric pH sensor based on mesoporous silica nanoparticles and Forster resonance energy transfer. Chem. Commun. 2010, 46, 8445-7. 102. Chen, Y.-P.; Chen, H.-A.; Hung, Y.; Chien, F.-C.; Chen, P.; Mou, C.-Y. Surface charge effect in intracellular localization of mesoporous silica nanoparticles as probed by fluorescent ratiometric pH imaging. RSC Adv. 2012, 2, 968. 103. Wang, D.; Nap, R. J.; Lagzi, I.; Kowalczyk, B.; Han, S.; Grzybowski, B. A.; Szleifer, I. How and why nanoparticle's curvature regulates the apparent pKa of the coating ligands. J. Am. Chem. Soc.2011, 133, 2192-7. 104. Sun, W.; Fang, N.; Trewyn, B.; Tsunoda, M.; Slowing, I.; Lin, V. Y.; Yeung, E. Endocytosis of a single mesoporous silica nanoparticle into a human lung cancer cell observed by differential interference contrast microscopy. Anal. Bioanal. Chem. 2008, 391, 2119-2125. 105. Das, D. D.; Sayari, A. Applications of pore-expanded mesoporous silica 6. Novel synthesis of monodispersed supported palladium nanoparticles and their catalytic activity for Suzuki reaction. J. Catal. 2007, 246, 60-65. 106. Trewyn, B. G.; Slowing, I. I.; Giri, S.; Chen, H.-T.; Lin, V. S. Y. Synthesis and Functionalization of a Mesoporous Silica Nanoparticle Based on the Sol–Gel Process and Applications in Controlled Release. Acc. Chem. Res. 2007, 40, 846-853. 107. Sun, J.; Zhang, H.; Tian, R.; Ma, D.; Bao, X.; Su, D. S.; Zou, H. Ultrafast enzyme immobilization over large-pore nanoscale mesoporous silica particles. Chem. Commun. 2006, 1322-1324. 108. Chung, T.-H.; Wu, S.-H.; Yao, M.; Lu, C.-W.; Lin, Y.-S.; Hung, Y.; Mou, C.-Y.; Chen, Y.-C.; Huang, D.-M. The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells. Biomaterials 2007, 28, 2959-2966. 109. Wu, S.-H.; Lin, Y.-S.; Hung, Y.; Chou, Y.-H.; Hsu, Y.-H.; Chang, C.; Mou, C.-Y. Multifunctional Mesoporous Silica Nanoparticles for Intracellular Labeling and Animal Magnetic Resonance Imaging Studies. ChemBio Chem. 2008, 9, 53-57. 110. Wu, S.-H.; Hung, Y.; Mou, C.-Y. Mesoporous silica nanoparticles as nanocarriers. Chem. Commun. 2011, 47, 9972-9985. 111. Trewyn, B. G.; Giri, S.; Slowing, I. I.; Lin, V. S. Y. Mesoporous silica nanoparticle based controlled release, drug delivery, and biosensor systems. Chem. Commun. 2007, 3236-3245. 112. Kao, K.-C.; Tsou, C.-J.; Mou, C.-Y. Collapsed (kippah) hollow silica nanoparticles. Chem. Commun. 2012, 48, 3454-3456. 113. Liu, S.; Cool, P.; Collart, O.; Van Der Voort, P.; Vansant, E. F.; Lebedev, O. I.; Van Tendeloo, G.; Jiang, M. The Influence of the Alcohol Concentration on the Structural Ordering of Mesoporous Silica: Cosurfactant versus Cosolvent. J. Phy. Chem. B 2003, 107, 10405-10411. 114. Tsou, C.-J.; Chu, C.-y.; Hung, Y.; Mou, C.-Y. A broad range fluorescent pH sensor based on hollow mesoporous silica nanoparticles, utilising the surface curvature effect. J. Mater. Chem. B 2013, 1, 5557-5563. 115. Li; Shi; Hua; Chen; Ruan; Yan. Hollow Spheres of Mesoporous Aluminosilicate with a Three-Dimensional Pore Network and Extraordinarily High Hydrothermal Stability. Nano Lett. 2003, 3, 609-612. 116. Li, J.; Liu, J.; Wang, D.; Guo, R.; Li, X.; Qi, W. Interfacially controlled synthesis of hollow mesoporous silica spheres with radially oriented pore structures. Langmuir 2010, 26, 12267-72. 117. Tsou, C.-J.; Hung, Y.; Mou, C.-Y. Hollow mesoporous silica nanoparticles with tunable shell thickness and pore size distribution for application as broad-ranging pH nanosensor. Micropor. Mesopor. Mater. 2014, 190, 181-188. 118. Mine, E.; Nagao, D.; Kobayashi, Y.; Konno, M. Solvent Effects on Particle Formation in Hydrolysis of Tetraethyl Orthosilicate. J. Sol-Gel Sci. Technol. 2005, 35, 197-201. 119. Tan, B.; Rankin, S. E. Dual Latex/Surfactant Templating of Hollow Spherical Silica Particles with Ordered Mesoporous Shells. Langmuir 2005, 21, 8180-8187. 120. Wang, D. P.; Zeng, H. C. Creation of Interior Space, Architecture of Shell Structure, and Encapsulation of Functional Materials for Mesoporous SiO2 Spheres. Chem. Mater. 2011, 23, 4886-4899. 121. Okamoto, M.; Huang, H. Formation of hollow silica spheres with ordered mesoporous structure by treatment with dimethyl carbonate for selective decomposition of mesoporous silica core. Micropor. Mesopor. Mater. 2012, 163, 102-109. 122. Cheng, S.-H.; Lee, C.-H.; Yang, C.-S.; Tseng, F.-G.; Mou, C.-Y.; Lo, L.-W. Mesoporous silica nanoparticles functionalized with an oxygen-sensing probe for cell photodynamic therapy: potential cancer theranostics. J. Mater. Chem. 2009, 19, 1252-1257. 123. Lu, F.; Wu, S.-H.; Hung, Y.; Mou, C.-Y. Size Effect on Cell Uptake in Well-Suspended, Uniform Mesoporous Silica Nanoparticles. Small 2009, 5, 1408-1413. 124. Wei, Y.; Jana, N. R.; Tan, S. J.; Ying, J. Y. Surface Coating Directed Cellular Delivery of TAT-Functionalized Quantum Dots. Bioconjugate Chem. 2009, 20, 1752-1758. 125. Lee, C.-H.; Cheng, S.-H.; Wang, Y.-J.; Chen, Y.-C.; Chen, N.-T.; Souris, J.; Chen, C.-T.; Mou, C.-Y.; Yang, C.-S.; Lo, L.-W. Near-Infrared Mesoporous Silica Nanoparticles for Optical Imaging: Characterization and In Vivo Biodistribution. Adv. Funct. Mater. 2009, 19, 215-222. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57609 | - |
dc.description.abstract | 酸鹼值在細胞生理系統中扮演極重要的角色,從細胞增生、胞吞作用(endocytosis)到細胞凋零都伴隨著酸鹼值改變,因此一個能精確偵測細胞內酸鹼值變化的偵測器是探討細胞生理學不可或缺的重要利器。近年來,具有高生物相容性、高表面積且易於在表面修飾各種官能基的中孔洞二氧化矽奈米粒子,在各研究領域都受到相當廣泛的討論。
在此研究中,我們建立了一個統合的細胞內單粒子追蹤與酸鹼值偵測系統,突破過去文獻中只能專一在其中一項技術的限制。在材料方面,利用將螢光分子fluorescein isothiocyanate (FITC) 與rhodamine B isothiocyanate (RITC) 以共縮合方式鑲嵌於二氧化矽中孔洞奈米粒子的結構當中,其中FITC在鹼性環境下螢光強度會增加,而RITC的螢光強度則不受環境酸鹼值影響,再利用其強度比值去做換算便能得知該環境的酸鹼度。本篇論文的第一部分我們使用三維空間即時單粒子追蹤技術,去追蹤癌細胞對於修飾螢光分子之中孔洞二氧化矽奈米粒子的吞噬行為與粒子所在環境的酸鹼值。透過觀察胞吞作用以及粒子脫離核內體(endosome)的過程,我們發現周遭環境酸鹼值改變與粒子運動行為有密切關連,而這些行為同時也受到細胞生理狀態的影響。論文第二部分則希望能改善過去的酸鹼值偵測器過於狹窄的偵測範圍,使其能準確地反映周遭環境變化。藉由合成具有中空結構和大小不一的表面孔洞的螢光中孔洞二氧化矽奈米粒子,我們利用這些曲率不一致的表面來影響FITC與材料表面帶正電荷官能基之間的距離,進而改變其酸解離常數(pKa)且增廣FITC螢光強度變化所涵蓋的pH值範圍,讓此奈米粒子成為具備極佳發展潛能的細胞pH偵測器。未來希望能將此細胞酸鹼值偵測器與追蹤技術應用於更多生物現象的觀察,以冀求對於細胞生理機制能有更進一步之了解。 | zh_TW |
dc.description.abstract | Intracellular pH plays many important roles in cell, including proliferation, apoptosis and endocytosis. Therefore, information of intracellular pH can help us to understand the cellular dysfunctions and the physical conditions of organelles. Since the discovery of mesoporous silica nanoparticle (MSN), such silica materials with controllable physical properties and excellent biocompatibility have received tremendous attention. Because the silanol groups on surface are available for further modification of diverse functional groups, MSN has been employed in drug delivery, intracellular imaging and sensing.
Herein, dye-leaded MSN is synthesized by co-condensing a pH-sensitive dye FITC and a reference dye RITC with silica source. By ratiometric method, one can correctly obtain the readout of this pH sensor. In the first part of the thesis, we demonstrate a 3D real time tracking technique which can record the trajectories of the fluorescent dye-loaded MSN (FMSN) internalized by cancer cell and the change of pH at the same time. The motion of FMSN and pH are proved highly correlative by observing the acidification after endocytosis and the increase of pH due to endosome escape. Moreover, the cellular dysfunction is shown responsible for the occurrence of endosome escape. In the second part, we synthesize hollow structured FMSN by adding hexane during the synthesis, which also leads to a broad pore size distribution. The different surface curvature due to various pore sizes affects not only the distance between FITC and positive-charged functional groups on the silica surface but the pKa value of FITC. As a result, we obtain a powerful pH sensor which owns a broad pH sensing range. We envision these researches can open up new designs of sensors and improve the knowledge of cellular process. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T06:53:55Z (GMT). No. of bitstreams: 1 ntu-103-R01223129-1.pdf: 5192758 bytes, checksum: e6b6a648fdc651f952e478848c0bbd5f (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 口試委員會審定書 #
謝誌 I 中文摘要 II Abstract III Table of Contents i List of Figures vi List of Tables xv List of Schemes xvi Chapter 1 General Introduction 1 1.1 Introduction to Mesoporous Silica Nanoparticle (MSN) 1 1.1.1 Synthesis Mechanism of MSN 3 1.1.2 Applications of MSN 6 1.2 Introduction to Hollow Mesoporous Silica nanoparticles (HMSNs) 9 1.2.1 Synthesis of HMSNs 9 1.2.2 Applications of HMSN 12 1.3 References 14 Chapter 2 Simultaneous 3D Single Particle Tracking and Ratiometric Local pH Detection in Cancer Cells 19 2.1 Introduction 19 2.2 Experimental Section 25 2.2.1 Materials 25 2.2.2 Characterization 27 I. Transmission Electron Microscopy (TEM) 27 II. Nitrogen Adsorption-Desoprtion Isotherms 27 III. Fluorescence Spectrum 28 IV. X-ray Diffraction Pattern 28 V. Dynamic Light Scattering (DLS) 28 VI. Zeta Potential 29 2.2.3 Synthetic Procedure 30 I. Preparation of Dye Conjugated APTMS 30 II. Synthesis of TA-FMSNs 30 III. Synthesis of THPMP-FMSNs 32 2.2.4 Fluorescence Microscope Setup 34 2.2.5 Calibration Curves 36 I. Z-Axial Position Calibration 36 II. pH Calibration 36 2.2.6 In Vitro Cell Studies 38 I. Cell Culture 38 II. Single Particle Tracking and Spectroscopy in Live Cell 39 III. Drug Treatment 39 2.3 Results and Discussions 40 2.4 Conclusion 69 2.5 References 70 Chapter 3 A Broad-range fluorescent pH Sensor Based on Hollow Mesoporous Silica Nanoparticles with Surface Curvature Effect 73 3.1 Introduction 73 3.1.1 Measuring Intracellular pH by Fluorescence 73 3.1.2 Broadening the Sensing Range of Nano-sized pH sensor 79 3.1.3 pH Biosensor Based on Mesoporous Silica Nanoparticles 82 3.2 Experimental Section 84 3.2.1 Materials 84 3.2.2 Characterization 86 I. Transmission Electron Microscopy (TEM) 86 II. Nitrogen Adsorption-Desoprtion Isotherms 86 III. Fluorescence Spectrum 87 IV. X-ray Diffraction Pattern 87 V. Dynamic Light Scattering (DLS) 87 VI. Scanning electron microscope (SEM) 88 VII. Zeta Potential 88 3.2.3 Synthetic Procedure 89 I. Preparation of Dye Conjugated APTMS 89 II. Synthesis of Dye-HMSN 89 III. Synthesis of Dye-MSN 90 IV. Synthesis of HMSN Using Ethanol (EX-s) 91 V. Synthesis of HMSN Using THF (TYDZ) 92 VI. Synthesis of Dye-loaded Mesoporous Products 93 3.2.4 In Vitro Cell Study 94 I. Cell Cuture 94 II. Incubation of HeLa cells with Dye-HMSNs 95 III. Confocal Microscope Study 95 IV. Establishment of Calibration Curves 96 3.3 Results and Discussion 97 3.3.1 Comparing Dye-MSN with Dye-HMSN 97 I. Results of Characterization 97 II. Hypothesis of the Broad pH-Sensing Capability 103 3.3.2 Mechanism Study 105 I. Characterization of the HMSNs 105 II. Solvent Effect 112 III. Broad-Ranging pH sensors 117 3.3.3 Intracellular pH study 126 3.4 Conclusions 131 3.5 References 132 Chapter 4 Conclusion 137 | |
dc.language.iso | en | |
dc.title | 應用修飾螢光分子之中孔洞二氧化矽奈米粒子進行癌細胞內酸鹼值偵測與三維空間即時追蹤 | zh_TW |
dc.title | Utilizing Fluorescent Dye Loaded Mesoporous Silica Nanoparticle for pH Sensing and 3D Real Time Tracking in Cancer Cell | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳培菱(Pei-Lin Chen),周必泰(Pi-Tai Chou) | |
dc.subject.keyword | 中孔洞二氧化矽,癌細胞,酸鹼值偵測器,三維空間即時追蹤,胞吞作用, | zh_TW |
dc.subject.keyword | Mesoporous silica,Cancer cell,pH sensor,3D Real Time Tracking,Endocytosis, | en |
dc.relation.page | 138 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-07-21 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 化學研究所 | zh_TW |
顯示於系所單位: | 化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 目前未授權公開取用 | 5.07 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。