合併補充益生菌及含魚油之綜合營養劑對BALB/c小鼠免疫的影響

Abstract

益生菌是指對宿主有正面效益的活性微生物，藉由活化腸道內的巨噬細胞或樹突細胞進而刺激或調節宿主的免疫系統；魚油藉由降低吞噬細胞活性、減少發炎激素分泌方式抑制過度或慢性的發炎反應。本實驗探討合併補充兩種具免疫調節能力的益生菌與含魚油的綜合營養劑對免疫調節的效果是否比單獨補充顯著。 本實驗檢測的益生菌為Lactobacilus rhamnosus及Bifidobacterium lactis之混合物；綜合營養劑除了魚油外含有多種微量營養素。實驗分成兩部分，分別為非特異性及特異性免疫反應。非特異性免疫反應實驗中，利用九週大的BALB/c小鼠，管餵樣品六週後觀察其先天性及非特異之適應性免疫指標，結果合併補充的組別較單一樣品補充顯著提升自然殺手細胞活性、提升血清總IgM、IgG及IgA含量及降低脾臟細胞IL-2分泌量。得知合併補充較具免疫調節能力後，接以進行特異性免疫反應實驗，同樣以九週大的BALB/c小鼠進行OVA致免模式，檢測指標除了有非特異性免疫指標，亦含OVA特異性抗體及脾臟細胞在OVA刺激下激素的檢測。結果顯示合併補充組別顯著促進B細胞增生、血清總IgG及IgA含量及脾臟細胞IL-10分泌量；血液吞噬細胞活性、T細胞增生有增加的趨勢，卻依然降低脾臟細胞IL-2分泌量。合併補充組別特異性免疫結果在血清OVA-specific IgG有較高趨勢，在OVA刺激下脾臟細胞IL-2分泌量顯著上升。 綜合以上結果得知合併補充益生菌及含魚油之綜合營養劑相較單一補充的組別，較能提升BALB/c小鼠免疫細胞的活化及免疫系統的調節能力，其中，最明顯的是提升血清總抗體含量，顯示小鼠抵抗致病原感染的能力及黏膜免疫都有提升的現象。

Probiotics is defined as the microbe that beneficially affect the host. By influencing the intestinal macrophage and dendritic cell, probiotics may stimulate the host immune response or promote the immune-modulation function. Fish oil alleviates the excessive and chronic inflammation by reducing the phagocytic activity of macrophage and pro-inflammatory cytokine level. Therefore, we wondered that whether the combination of probiotics and nutrition supplement containing fish oil would multiple the effects of immune-modulatory function. In this study, the mixture of Lactobacilus rhamnosus and Bifidobacterium lactis were used as the source of probiotics and the nutrition supplement contains fish oil and various micronutrients. There were two parts in this experiment: non-specific and specific immune response. In the first part, nine-week-old BALB/c female mice were tube-fed with different contents including probiotics alone, nutrition supplement alone or combined sample for 6 weeks and followed by performing sacrifice to measure the innate and non-specific adaptive immune indicators. The results showed that combined-sample group significantly increased activity of NK cell, elevated total IgM, IgG, IgA in the serum and reduced IL-2 secretion of splenocytes. Knowing that the immune-modulatory ability of combined-sample group, we continued with the next part. In the experiment, nine-week-old BALB/c female mice were not only fed by different sample for 9 weeks but also immunized with OVA, followed by sacrifice. The innate immune responses and adaptive immune responses including OVA-specific and non-specific were examined. The results showed that combined-sample group significantly induced B cell proliferation, elevated total IgG, IgA in the serum, and significantly increased IL-10 but reduced IL-2 secretion of splenocytes. Combined-sample group also tend to induce phagocytic activity and T cell proliferation. In the OVA-specific indicators, combined-sample group tend to increase OVA-specific IgG and significantly increased IL-2 secretion with OVA- stimulated splenocytes. In conclusion, combined-sample group is the most remarkable to modulate the immune system within other single-sample groups, especially the elevated level of total antibodies. Supporting that supplied BALB/c mice with probiotics combined nutrition supplement containing fish oil may benefit the immune system than the single sample group, which may avoid the extracellular pathogen invasion and promote mucosal immunity.