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標題: | 透明質酸-層黏連蛋白-褐藻酸細胞載體應用於脂肪組織工程之研究 The Study of HA-Laminin-Alginate Bead as Cell Carrier for Adipose Tissue Engineering |
作者: | Yen-Yu Chen 陳彥宇 |
指導教授: | 林峰輝(Feng-Huei Lin) |
共同指導教授: | 戴浩志(Hao-Chih Tai) |
關鍵字: | 脂肪組織工程,支架,乳房重建, Adipose tissue engineering,Scaffold,Breast reconstruction, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 目前臨床上常使用自體脂肪移植的方式進行乳房重建手術,直接抽脂後注入乳房中,雖然具有相當多優點,但仍有40至60 %的體積會在植入後鈣化或脂肪再吸收,造成患者需要重覆手術才能維持理想的體積。本研究提出一種新材料--透明質酸-層粘連蛋白-褐藻酸,能夠做為脂肪組織工程的細胞載體,應用於乳房重建手術中可以改善現行臨床上脂肪再吸收的問題。研究結果顯示,透明質酸-層粘連蛋白-褐藻酸能夠製作出體積約1.886±0.07 mm3的微球細胞載體,具有良好的質傳效率,降解至第六十天仍有60 %的質量,並且材料本身不具毒性,能夠促進細胞貼附、增生及維持分化,在第二週時不論是細胞活性或是維持分化能力都顯著高於一般的褐藻酸載體。於動物體內試驗也證明,透明質酸-層粘連蛋白-褐藻酸能夠同時促進脂肪分化及血管新生,避免脂肪再吸收及鈣化,甚至第三週時能再增加42 %的體積,證明透明質酸-層粘連蛋白-褐藻酸相當具有潛力應用於脂肪組織工程及改善乳房重建手術。 In recent years, autologous fat transplantation is commonly used in clinical breast reconstruction surgery. Fat tissue injected into the breast directly after liposuction, it has many benefits, however, tissue resorption or calcification result in 40–60% of the graft volume loss. It cause patient must accept reoperation for maintain identical volume. In current study present a new material-- Hyaluronic acid-Laminin-Alginate as a cell carrier for used in adipose tissue engineering and improve breast reconstruction surgery by avoid tissue resorption. Results showed that Hyaluronic acid-Laminin-Alginate could create a 1.886±0.07 mm3 cell carrier, has a suitable mass transfer efficiency. Also, it could remain 60 % weight after 60 days degraded, non-cytotoxicity, could promote cells attachment, proliferation and differentiation. Hyaluronic acid-Laminin-Alginate has significantly higher cell proliferation and adipogenesis than alginate in 2 weeks data. In vivo animal study also prove that Hyaluronic acid-Laminin-Alginate could promote both adipogenesis and angiogenesis, prevent resorption and calcification, and increase 42 % volume after 3 weeks implanted. Hyaluronic acid-Laminin-Alginate has the potential to be applied in adipose tissue engineering and improve breast reconstruction surgery. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56842 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學工程學研究所 |
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ntu-103-1.pdf 目前未授權公開取用 | 4.4 MB | Adobe PDF |
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