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標題: | 利用布朗運動檢測方法於奈米薄膜濃縮晶片之技術 A Biosensing Method Based on Nano-Particles’ Brownian Motion Applied to Nanofluidic Preconcentration Chip |
作者: | Heng-Chia Chiou 邱珩嘉 |
指導教授: | 沈弘俊(Horn-Jiunn Sheen) |
共同指導教授: | 田維誠(Wei-Cheng Tian) |
關鍵字: | 布朗運動,奈米粒子,離子選擇性,濃度極化,氣閥,微粒子追蹤測速儀, Nafion,Brownian motion,ion-selective membrane,ion concentration polarization (ICP),pneumatic valve,micro-Particle Tracking Velocimetry (micro-PTV), |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 本研究開發一新的檢測系統,使用微奈米濃縮晶片對生物樣本進行預濃縮後,利用氣閥捕捉濃縮區塊,再進行布朗運動的檢測分析。主要的微奈米流體濃縮晶片,是使用Nafion奈米薄膜作為離子選擇性通道,又可稱為濃縮器(precon- centrator),施加一電位差於具有離子選擇性(ion-selective)效果的Nafion薄膜上可產生濃度極化效應(ion concentration polarization),於高電位處產生離子空乏區(ion depletion region)。此時施加偏壓可產生第二種電滲透流(electroosmosis of second kind),可在高電位處的空乏區旁產生原本樣本濃度的105~106倍的濃縮區塊(preconcentration plug),可以大幅降低濃度的生物樣本的偵測下限。達到低濃度生物標記(biomarker)的偵測,使用氣閥(pneumatic valve)將濃縮區塊捕捉後,結合布朗運動,利用抗原與抗體間之專一性(Specific Relationship)辨認作為檢測機制。
本文詳述使用Nafion奈米薄膜濃縮晶片的設計與驗證,包括Nafion的導電率與晶片電阻模型的驗證、以及氣閥捕捉濃縮區塊的成效。使用LabVIEW架設迴路電流監測系統觀測電流與濃縮之間的特性,並以微粒子追蹤測速儀(micro-Particle Pracking Velocimetry, micro-PTV)進行布朗運動的量測與分析。 本研究所開發的檢測方法具有降低生物樣本偵測下限,提高檢測靈敏度的能力,因此將來可以使用在不同的低濃度生物樣本上,以利後端的生物檢測。 In this thesis, we have developed a novel detection method based on capturing preconcentrated plug preconcentrated by preconcentration chip and Brownian motion detection. Applying voltage drop to the preconcentrator which use Nafion as ion-selective membrane can generate ion concentration polarization (ICP) effect. The electroosmosis of second kind which generate by applying the bias voltage, force bulk solution to aggregate the preconcentration plug near the depletion region. Using the Brownian motion which analyze the characteristic of antigen-antibody interactions as detection mechanism to detect the preconcentration plug captured by pneumatic valve. The design and the validation methods and processes of nanofluidic preconcentration chips and pneumatic valve were proposed starting from validation of resistive models, test of ion-selective membrane and test of Brownian motion by micro-Particle Tracking Velocimetry (micro-PTV), to get the onset of detection mechanism. In summary, the presented nanofluidic preconcentrator and pneumatic valve demonstrates biological sample can be preconcentrated in short time and the plug can be captured by valve. With the analysis of loop currents and Brownian motion detection, various low biological sample could be demonstrated in the future. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56803 |
全文授權: | 有償授權 |
顯示於系所單位: | 應用力學研究所 |
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