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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 孫岩章(En-Jang Sun) | |
dc.contributor.author | Ji-Fang Cheng | en |
dc.contributor.author | 鄭及昉 | zh_TW |
dc.date.accessioned | 2021-06-16T05:47:11Z | - |
dc.date.available | 2014-08-21 | |
dc.date.copyright | 2014-08-21 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-11 | |
dc.identifier.citation | 參考文獻
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56767 | - |
dc.description.abstract | 本研究之目的為臭氧生物監測技術之應用與機制。研究分為四個部份:(一)利用組織培養並配合無臭氧環境下馴化組培苗,以建立本土指標植物光果龍葵。(二)利用T8菸草監測室內外臭氧。(三)探討影響Bel-W3菸草指標植物臭氧敏感性之因子。(四)利用臭氧指標植物探討臭氧病害及植物免疫間之關係。結果顯示:(一)野生龍葵之臭氧敏感性歧異度極大,以組織培養並配合無臭氧環境下馴化之龍葵組培苗對臭氧有極高的敏感性且變異性小。(二)臭氧超敏感T8菸草以迷你化平盤方式栽培,並配合小型生態箱之運用,可成功監測室內臭氧。在台大校園內六定點中,以男一舍影印間之臭氧濃度最高。在台北市區校園內,以文化國小之臭氧濃度最高。(三)臭氧前處理會降低Bel-W3菸草之臭氧敏感性,且經慢性臭氧處理過之老葉,可保護新生之葉免於後續之臭氧急性危害。(四)利用非親合性細菌引發植物過敏反應之實驗中發現,對臭氧敏感之Bel-W3菸草,其對非親合性細菌之過敏反應,比臭氧耐受品種Bel-B菸草之過敏反應強。且在葉齡與過敏反應相關性實驗中發現,Bel-W3菸草之老葉對非親合性細菌之過敏反應,比年輕葉之過敏反應強。結果顯示Bel-W3菸草對非親合性細菌之過敏反應其強度表現與對臭氧之反應一致;臭氧同時具有為害植物及誘導植物免疫的雙重功能,其作用機制相當複雜。研究結果顯示臭氧指標植物是用於研究臭氧病害及植物免疫間關係之良好材料。 | zh_TW |
dc.description.abstract | The aims of the study were to explore the applications and mechanisms of the ozone biomonitoring techniques. There were four parts in this study: (1) Black nightshade plantlets from tissue culture are acclimatized in charcoal-filtered air as a bio-indicator for ozone; (2)The use of tobacco T8 indicator plant to monitor the indoor ozone; (3)Factors affecting ozone sensitivity of tobacco Bel-W3 seedlings; and (4) The use of tobacco T8 indicator plant to explore the relationship between ozone injuries and hypersensitive reactions of plants. Results showed: (1) The tissue culture technique is a successful propagation method for providing black nightshades with more sensitive and uniform responses to ozone. An charcoal-filtered or ozone-free ambient air used during plantlet acclimatization can increase the O3 sensitivity. (2) Among the six monitoring sites in the NTU campus, the leaf injury index percentage (LII%) of T8 seedlings were the largest in the copying room of the dormitory. Among the six classrooms in Taipei City, the foliar injuries of the T8 seedlings in Wen-Hua Elementary School were the most serious. (3) Pre-exposure to O3 is a critical factor that decrease the O3 sensitivity and chronically exposed old leaves help protect young leaves from acute injuries when later exposed to O3. (4) Hypersensitive reactions (HR) can be induced by Xanthomons campestris pv. vesicatoria (Xcv ), a non-host bacterium, on two tobacco (Nicotiana tabacum) cultivars with different sensitivity of ozone. While the O3-sensitive tobacco cv. Bel-W3 displayed significantly higher HR intensity than the ozone-resistant tobacco cv. Bel-B. In an age-related study, the older leaves of O3-sensitive tobacco cv. Bel-W3 displayed significantly higher HR intensity than younger leaves. Ozone can be phytotoxic to plants or as an elicitor as well, and both the mechanisms are complex. This study represented that the ozone indicator plants were an ideal model plant to explore the relationship between ozone injuries and hypersensitive reactions of plants. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T05:47:11Z (GMT). No. of bitstreams: 1 ntu-103-D94633001-1.pdf: 4170034 bytes, checksum: b0cc278c73847647fe346032d1dc09fa (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 第一章 總論………………………………………………………...1
第一節 臭氧污染………………………………………………………….....1 第二節 臭氧對動植物健康之影響…………………………………………..2 一、臭氧對植物之影響……………………………………………............2 二、臭氧對人體之危害……………………………………………………2 第三節 臭氧指標植物與生物監測技術……………………………………..3 第四節 臭氧與植物防禦系統………………………………………………..3 第二章 臭氧指標植物之開發:建立光果龍葵為本土臭氧指標植物………………………………………………………………………………....7 第一節 前人研究...............................................................................................7 一、臭氧指標植物之原理及應用………………………………………….7 二、開發本土臭氧指標植物光果龍葵…………………………………….9 第二節 材料方法……………………………………………………………..11 一、田間調查………………………………………………………………11 二、初次篩選(primary screen)……………………………………………..11 三、人工模擬熏氣…………………………………………………………11 四、病害評估……………………………………………………………....12 五、組織培養………………………………………………………………12 第三節 結果與討論…………………………………………………………..15 一、田間調查………………………………………………………………15 二、野生龍葵之臭氧敏感性分析…………………………………………18 三、龍葵組培苗臭氧敏感度測試…………………………………………21 第三章 臭氧指標植物菸草的應用……………………………………28 第一節 前人研究……………………………………………………………..28 一、指標植物種植之標準化 …………………………………………..28 二、室內臭氧(indoor ozone)……………………………………………30 三、超敏感型T8煙草……………………………………………………30 第二節 材料方法…………………………………………………………..31 一、利用七葉齡T8菸草監測台北市區校園大氣臭氧………………..31 二、利用21日齡之T8菸草幼苗監測室內臭氧……………………....32 第三節 結果………………………………………………………………..35 一、利用七葉齡T8菸草監測台北市區校園大氣臭氧………………..35 二、利用21日齡之T8菸草幼苗監測室內臭氧……………………........35 第四節 討論………………………………………………………………..40 第四章大氣臭氧對指標植物Bel-W3菸草臭氧敏感度之影響....49 第一節 前人研究…………………………………………………………..49 一、Bel-W3菸草品種的起源及應用…………………………………...49 二、臭氧對植物之影響………………………………………………...49 三、臭氧致病機制…………………………………………………….....50 第二節 材料方法…………………………………………………………...52 一、21日齡菸草迷你化平盤(mini-kit)之置備………………………….52 二、老葉對新葉之臭氧敏感性影響…………………………………….52 第三節 結果…………………………………………………………….......53 一、慢性臭氧前處理對菸草敏感性之影響…………………………….53 二、老葉對新生葉臭氧敏感性之影響…………………………………..53 三、急性臭氧前處理對臭氧敏感性之影響……………………………..53 第四節 討論與結論……………………………………………………........60 第五章 臭氧與植物免疫:利用指標植物研究植物防禦系統..61 第一節 前人研究…………………………………………………………61 一、臭氧與PCD反應…………………………………………………62 二、臭氧與植物抗菌素………………………………………………..63 三、臭氧與訊息傳導物質……………………………………………..63 四、臭氧與植物抗氧化物……………………………………………..64 五、臭氧與病原相關蛋白……………………………………………..65 第二節 材料方法…………………………………………………………66 一、菸草幼苗培育……………………………………………………..66 二、菌種材料及接種程序……………………………………………..66 三、過敏反應試驗……………………………………………………..66 第三節 結果與討論……………………………………………………....67 第六章 結論……………………………………………………………....73 參考文獻……………………………………………………………………75 | |
dc.language.iso | zh-TW | |
dc.title | 臭氧生物監測技術之研究 | zh_TW |
dc.title | Studies on Biomonitoring Technology for Ozone | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 葉德銘(Der-Ming Yeh),張育森(Yu-Sen Chang),王亞男(Ya-Nan Wang),鄭福田(Fu-Tien Jeng),張艮輝(Ken-Hui Chang) | |
dc.subject.keyword | 臭氧生物監測,組織培養,菸草,甜椒細菌性斑點病菌,過敏反應, | zh_TW |
dc.subject.keyword | Ozone biomonitoring,tissue culture,tobacco,Xanthomons campestris pv. vesicatoria,hypersensitive reactions, | en |
dc.relation.page | 89 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-11 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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