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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李嗣涔(SC Lee) | |
| dc.contributor.author | Shin-Chi Yeh | en |
| dc.contributor.author | 葉欣綺 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:44:10Z | - |
| dc.date.available | 2015-08-25 | |
| dc.date.copyright | 2014-08-25 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-11 | |
| dc.identifier.citation | [1] Noordhuis, M.G., et al., Prognostic Cell Biological Markers in Cervical Cancer Patients Primarily Treated With (Chemo)radiation: A Systematic Review. International Journal of Radiation Oncology*Biology*Physics. 79(2): p. 325-334(2011).
[2] GM Clifford, JS Smith, M Plummer, N Mun ̃oz, S Franceschi. Human papillomavirus types in invasive cervical cancer worldwide: a meta-analysis. British Journal of Cancer 88, 63 – 73(2003). [3] Medeiros, L.R., et al., Efficacy of Human Papillomavirus Vaccines: A Systematic Quantitative Review. International Journal of Gynecological Cancer. 19(7): p. 1166-1176 10.1111/IGC.0b013e3181a3d100(2009). [4] Walboomers, J.M.M., et al., Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. The Journal of Pathology. 189(1): p. 12-19(1999). [5] Woodman, C.B.J., S.I. Collins, and L.S. Young, The natural history of cervical HPV infection: unresolved issues. Nat Rev Cancer. 7(1): p. 11-22(2007). [6] Lesile R. Schover, Michael Fife, Msw, David. Sexual Dysfunction and Treatment for Early Stage Cervical Cancer. Cancer. 63:204-212(1989). [7] Richard Roden, T.-C. Wu. How will HPV vaccines affect cervical cancer? Nature Reviews Cancer. doi:10.1038/nrc1973(2006). [8] Lehman, T.A., et al., p53 mutations in human immortalized epithelial cell lines. Carcinogenesis. 14(5): p. 833-9(1993). [9] Brown, C.L., The immortal life of henrietta lacks. J Health Polit Policy Law. 37(6): p. 1141-6(2012). [10] John R. Masters. Hela cells 50 years on: the good, the bad and the ugly. Nature Reviews Cancer, 2002, doi:10.1038/nrc774 [11] Maycock P (Ed). PV Energy System. PV NEWS 20(3), 2 (2001). [11] Mark Hill. Cell biology lecture, University of New South Wales, Australia. [12] Eric Daugas., et al., Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14, 729-739 (2000). [13] Hamblin, M.R. and T.N. Demidova, Mechanisms of low level light therapy. p. 614001-614001(2006). [14] Karu, T.I., Mitochondrial Signaling in Mammalian Cells Activated by Red and Near-IR Radiation. Photochemistry and Photobiology. 84(5): p. 1091-1099(2008). [15] M. W. Tsai, T. H. Chuang, C. Y. Meng, Y. T. Chang, and S. C. Lee, High performance midinfrared narrowband plasmonic thermal emitter. App. Phys. Lett. 89, 173128-173130(2006) [16] T.H. Chuang, M. W. Tsai, Y. T. Chang, and S. C. Lee, Remotely coupled surface plasmons in a twocolored plasmonic thermal emitter. App. Phys. Lett.89,173128-173130(2006) [17] M. U. Pralle, N. Moelders, M. P. McNeal, I. Puscasu, A. C. Greenward, J. T. Daly, E. A. Johnson, T. George, D. S. Choi, I. El-kady, and R. Biswas, Photonic crystal enhanced narrow-band infrared emitters. Appl. Phys. Lett. 81, 4685-4687(2002) [18] R. H. Ritchie, Phys. Rev. 106, 874−881 (1957). [19] H. Raether, Surface Plasmons (Springer-Verlag, Berlin, 1988). [20] C. J. Powell, J. B. Swan, Phys. Rev. 118, 640 (1960). [21] William L. Barnes, Alain Dereux and Thomas W. Ebbesen, Nature (London) 424, 824 (2003). [22] E. Kretschmann and H. Raether, Z. Naturforsch. A 23, 2135-2136 (1968). [23] Y.T. Chang, T.H. Chuang, M.W. Tsai, L.C. Chen, and S.C. Lee. Dispersion relation of Al/Si surface plasmon in hexagonally ordered aluminum hole arrays. Journal of Applied Physics 101, 054305 (2007). [24] H M Davey and D B Kell, Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiology and Molecular Biology Reviews. 60(4):641(1996) [25] Ruth M. Kluck, Ella Bossy-Wetzel, Douglas R. Green,* Donald D. Newmeyer*, The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis. Science 275, 1132 (1997) [26] S. T., Reers M., Mottola-Hartshorn C., Lin M., Chen A., Smith T. W., Steele G. D. Jr., Chen L. B. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate -forming lipophilic cation JC-1. Proc. Nat. Acad. Sci. U.S.A. 88, 3671-3675 (1991). [27] Chun-Chi Liang, Ann Y Park & Jun-Lin Guan, In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, doi:10.1038 (2007). [28] Vikas Malhotra, Michael C. Perry, Classical Chemotherapy: Mechanism, Toxicities and Theraputic Window. Cancer Biology & Therapy (2003) [29] Bernhard Lipper, Wiley-VCH, Weinheim, Developments in Cisplatin Research. Platinum Metals Rev: 103-104 (1999) [30] Rebecca A. Alderden , Matthew D. Hall and Trevor W. Hambley, The Discovery and Development of Cisplatin. J. Chem. Educ.. (2006) [31] PATRICK J. LOEHRER, M.D.; and LAWRENCE H. EINHORN, M.D, Cisplatin. ACP (1984) [32] Kristoph A. Klein, The metastasis cascade. Science 321 (5897), doi:10.1126/science.1164853 (2008) [33] Mathias Felix Leber, Thomas Efferth, Molecular principles of cancer invasion and metastasis. International Jurnal of Oncology, DOI:10.3892/ijo_00000214 (2009) [34] Christine L. Chaffer and Robert A. Weinberg, A perspective on cancer cell metastasis. Science 331, 1559, DOI: 10.1126/science.1203543 (2011) | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56719 | - |
| dc.description.abstract | 此研究的目的是用本實驗室所研發出表面電漿子紅外線發射器，利用其所發出的窄頻寬紅外線去照射子宮頸癌細胞48小時，並且使用生物分析方法測量癌細胞受紅外光刺激所造成的反應，主要探討是否會造成子宮頸癌細胞粒線體膜電位崩壞及是否會刺激下游的細胞走自我凋亡途徑。在本研究使用的紅外光發射器，是依據表面電漿子原理制作，此紅外光源結構是在矽基板上鍍上銀/二氧化矽/銀三層薄膜，即是金屬/絕緣層/金屬（MIM）三明治結構的薄膜。而最上層的金屬銀表面是使用光罩並利用曝光技術所制作的週期性排列孔洞藉以調變本實驗所需要的特定波長。透過電流加熱，此結構便會發出窄頻寬高功率的紅外光。將子宮頸癌細胞經過不同波長之紅外光照射48小時後，得知3~3.8 μm和3.8 μm波長照射下，癌細胞生長明顯受到抑制並且會抑制其細胞移動。 | zh_TW |
| dc.description.abstract | The purpose of this research is to study the infrared irradiation effect on the cervical cancer cells Hela growth and migration. The plasmonic thermal emitter with broad bandwidth (3~3.8 μm) and narrow emission waveband (3.8, 4.4, 4.8 μm) were designed and used to illuminate the cells for 48 hours to investigate the specific wavelength that affects the cell expression most significantly. Infrared emitters used in this study are fabricated based on the theory of surface plasmon. The infrared source can be achieved by heating the triple layer structure which consists of a SiO2 layer sandwitched between two Ag films on the silicon substrate, it is called metal/dielectric/metal (MIM) structure. The top Ag layer is perforated by periodic hole array, and the emission wavelength can be altered and selected by changing the lattice constant and diameter of the hole arrays.
It is found that the growth of Hela cell responds the most after illumination by PTE with peak wavelength at 3~3.8 μm and 3.8 μm, the growth of Hela cell was inhibited significantly. Besides, IR may regulate mitochondrial membrane potential, activate the downstream of apoptosis to decrease cell viability. And it also showed that 3~3.8 μm and 3.8 μm decreased the cell movement mostly after 48h IR exposure. In general, after infrared illumination under specific wavelengths, hela cells can be diminished in vitro. The study implies that IR illumination can prohibit the cell growth and promote cell apoptosis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:44:10Z (GMT). No. of bitstreams: 1 ntu-103-R00945034-1.pdf: 6723677 bytes, checksum: 3ee0442e5fd5a4fbddac9968257e010c (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 口試委員審定書 I
誌謝 II 摘要 III Abstract IV Chapter 1 Introduction 1 1.1 Introduction of Human Cervical Cancer cell……….…….…… 1 1.2 Immortal Cell Line Hela Cell …………………………………....3 1.3 Purpose of This reserch 5 1.4 Framework of Thesis 7 Chapter 2 The Fundamentals of Plasmonic Thermal Emitters 9 2.1 The Fundamentals of Surface plasmons 9 2.1.1 Surface plasmons on smooth surface 14 2.1.2 Surface plasmons on the surface with hole arrays… 15 2.1.3 Dispersion relation of surface plasmon in hexagonally ordered hole arrays 20 2.2 Process Flow… 23 2.2.1 Fabrication processes of metal hole arrays 23 2.1.1 Fabrication processes of plasmonic thermal emitter 26 2.3 Measureing Systems 30 2.3.1 Introduction of FTIR ………………………………………….……....29 2.3.2 Thermal emitter chamber ………………………………….….……....31 2.4 Incubator for cell illuminated by infrared 32 Chapter 3 Experimental and Analysis of Infrared Irradiation Effect 34 3.1 Experimental Setup 34 3.2 Materials and Experimental Process 40 3.2.1 Cell culture 40 3.2.2 WST-1 assay 40 3.2.3 Measurement of apoptosis 46 3.2.2 Cell mitochindrial membrane potential analysis 51 3.2.2 In vitro wound healing assay 56 Chapter 4 Effect of Narrow Bandwidth Infrared Radiation on Cancer Cells Hela Solar Cells 59 4.1 Cancer cells Hela illuminated by 3.8~4.8 μm Infrared Light 59 4.2 IR Exposure Experiments #1 (λp = 3.8 μm) 61 4.2.1 WST-1 61 4.2.2 Measurement of apoptosis 62 4.2.3 Cell mitochondrial membrane potential analysis 64 4.3 IR Exposure Experiments #2 (λp = 4.4 μm) 66 4.2.1 WST-1 66 4.2.2 Measurement of apoptosis 67 4.2.3 Cell mitochondrial membrane potential analysis 68 4.4 IR Exposure Experiments #3 (λp = 4.8 μm) 70 4.2.1 WST-1 70 4.2.2 Measurement of apoptosis 71 4.2.3 Cell mitochondrial membrane potential analysis 73 4.5 Analysis of In Vitro Wound Healing Assay 74 4.6 3~3.8 and 3.8 μm with Cisplatin 77 4.6.1 Introduction of Cisplatin 77 4.6.2 Measurement of apoptosis 78 Chapter 5 Conclusion 80 References 81 | |
| dc.language.iso | zh-TW | |
| dc.subject | 子宮頸癌細胞 | zh_TW |
| dc.subject | 表面電漿子紅外線發射器 | zh_TW |
| dc.subject | 窄頻 | zh_TW |
| dc.subject | 自我凋亡 | zh_TW |
| dc.subject | specific waveband | en |
| dc.subject | Infrared irradiation | en |
| dc.subject | plasmonic thermal emitter | en |
| dc.subject | apoptosis | en |
| dc.subject | cervical cancer cells Hela | en |
| dc.title | 窄頻紅外線照射對子宮頸癌細胞Hela生長之影響 | zh_TW |
| dc.title | Effect of Narrow Bandwidth Infrared Radiation on the Cervical Cancer Cells Hela Expressions | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許博欽,阮雪芬,謝旭亮 | |
| dc.subject.keyword | 表面電漿子紅外線發射器,窄頻,子宮頸癌細胞,自我凋亡, | zh_TW |
| dc.subject.keyword | Infrared irradiation,plasmonic thermal emitter,specific waveband, cervical cancer cells Hela,apoptosis, | en |
| dc.relation.page | 86 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-11 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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