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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56595完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張百恩(Bei-En Chang) | |
| dc.contributor.author | Kun-Han Yang | en |
| dc.contributor.author | 楊坤翰 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:36:47Z | - |
| dc.date.available | 2019-10-09 | |
| dc.date.copyright | 2014-10-09 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-13 | |
| dc.identifier.citation | Ando, Z., et al., 2005. Slc12a2 is a direct target of two closely related homeobox proteins, Six1 and Six4. FEBS J. 272, 3026-41.
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Genomic cloning and characterization of the human homeobox gene SIX6 reveals a cluster of SIX genes in chromosome 14 and associates SIX6 hemizygosity with bilateral anophthalmia and pituitary anomalies. Genomics. 61, 82-91. Goodrich, L. V., 2005. Hear, hear for the zebrafish. Neuron. 45, 3-5. Grifone, R., et al., 2005. Six1 and Six4 homeoproteins are required for Pax3 and Mrf expression during myogenesis in the mouse embryo. Development. 132, 2235-49. Harris, S., et al., 1996. Myotonic dystrophy: will the real gene please step forward! Hum Mol Genet. 5 Spec No, 1417-23. Islam, M. E., et al., 2006. Three enhancer regions regulate gbx2 gene expression in the isthmic region during zebrafish development. Mech Dev. 123, 907-24. Jean, D., et al., 1999. Six6 (Optx2) is a novel murine Six3-related homeobox gene that demarcates the presumptive pituitary/hypothalamic axis and the ventral optic stalk. Mech Dev. 84, 31-40. Jeong, Y., et al., 2006. A functional screen for sonic hedgehog regulatory elements across a 1 Mb interval identifies long-range ventral forebrain enhancers. Development. 133, 761-72. Kawakami, K., et al., 1996a. Structure, function and expression of a murine homeobox protein AREC3, a homologue of Drosophila sine oculis gene product, and implication in development. Nucleic Acids Res. 24, 303-10. Kawakami, K., et al., 1996b. Identification and expression of six family genes in mouse retina. FEBS Lett. 393, 259-63. Kawakami, K., et al., 2000. Six family genes--structure and function as transcription factors and their roles in development. Bioessays. 22, 616-26. Klesert, T. R., et al., 2000. Mice deficient in Six5 develop cataracts: implications for myotonic dystrophy. Nat Genet. 25, 105-9. Kobayashi, M., et al., 2000. Expression of three zebrafish Six4 genes in the cranial sensory placodes and the developing somites. Mech Dev. 98, 151-5. Kobayashi, M., et al., 1998. Overexpression of the forebrain-specific homeobox gene six3 induces rostral forebrain enlargement in zebrafish. Development. 125, 2973-82. Kochhar, A., et al., 2007. Branchio-oto-renal syndrome. Am J Med Genet A. 143, 1671-8. Laclef, C., et al., 2003. Thymus, kidney and craniofacial abnormalities in Six 1 deficient mice. Mech Dev. 120, 669-79. Loosli, F., et al., 1998. Six3, a medaka homologue of the Drosophila homeobox gene sine oculis is expressed in the anterior embryonic shield and the developing eye. Mech Dev. 74, 159-64. Lopez-Rios, J., et al., 1999. Six9 (Optx2), a new member of the six gene family of transcription factors, is expressed at early stages of vertebrate ocular and pituitary development. Mech Dev. 83, 155-9. Ohto, H., et al., 1999. Cooperation of six and eya in activation of their target genes through nuclear translocation of Eya. Mol Cell Biol. 19, 6815-24. Ohto, H., et al., 1998. Tissue and developmental distribution of Six family gene products. Int J Dev Biol. 42, 141-8. Oliver, G., et al., 1995a. Six3, a murine homologue of the sine oculis gene, demarcates the most anterior border of the developing neural plate and is expressed during eye development. Development. 121, 4045-55. Oliver, G., et al., 1995b. Homeobox genes and connective tissue patterning. Development. 121, 693-705. Ozaki, H., et al., 2004. Six1 controls patterning of the mouse otic vesicle. Development. 131, 551-62. Ozaki, H., et al., 2001. Six4, a putative myogenin gene regulator, is not essential for mouse embryonal development. Mol Cell Biol. 21, 3343-50. Park, B. K., et al., 2004. Intergenic enhancers with distinct activities regulate Dlx gene expression in the mesenchyme of the branchial arches. Dev Biol. 268, 532-45. Prince, V. E., Pickett, F. B., 2002. Splitting pairs: the diverging fates of duplicated genes. Nat Rev Genet. 3, 827-37. Roessler, E., Muenke, M., 1998. Holoprosencephaly: a paradigm for the complex genetics of brain development. J Inherit Metab Dis. 21, 481-97. Ruf, R. G., et al., 2004. SIX1 mutations cause branchio-oto-renal syndrome by disruption of EYA1-SIX1-DNA complexes. Proc Natl Acad Sci U S A. 101, 8090-5. Sarkar, P. S., et al., 2004. Six5 is required for spermatogenic cell survival and spermiogenesis. Hum Mol Genet. 13, 1421-31. Schlosser, G., 2006. Induction and specification of cranial placodes. Dev Biol. 294, 303-51. Seo, H. C., et al., 1999. Six class homeobox genes in drosophila belong to three distinct families and are involved in head development. Mech Dev. 83, 127-39. Seo, H. C., et al., 1998a. Expression of two zebrafish homologues of the murine Six3 gene demarcates the initial eye primordia. Mech Dev. 73, 45-57. Seo, H. C., et al., 1998b. Transient expression of a novel Six3-related zebrafish gene during gastrulation and eye formation. Gene. 216, 39-46. Seo, H. C., et al., 1998c. A zebrafish Six4 homologue with early expression in head mesoderm. Biochim Biophys Acta. 1442, 427-31. Spitz, F., et al., 1998. Expression of myogenin during embryogenesis is controlled by Six/sine oculis homeoproteins through a conserved MEF3 binding site. Proc Natl Acad Sci U S A. 95, 14220-5. Toy, J., Sundin, O. H., 1999. Expression of the optx2 homeobox gene during mouse development. Mech Dev. 83, 183-6. Xu, P. X., et al., 2003. Six1 is required for the early organogenesis of mammalian kidney. Development. 130, 3085-94. Zheng, W., et al., 2003. The role of Six1 in mammalian auditory system development. Development. 130, 3989-4000. Zou, D., et al., 2006. Patterning of the third pharyngeal pouch into thymus/parathyroid by Six and Eya1. Dev Biol. 293, 499-512. Zuber, M. E., et al., 1999. Giant eyes in Xenopus laevis by overexpression of XOptx2. Cell. 98, 341-52. 呂智凱, 藉由綠色螢光蛋白報導基因研究斑馬魚Six1.1基因啟動子之顱顏組織專一性表現. 國立臺灣大學醫學院口腔生物科學研究所口腔細胞生物學組碩士論文, 2008. 林育珍, 以Tol2跳躍基因系統剖析斑馬魚six1,six6 及人類 DMP1之組織專一性促進子之功能，並以此系統在斑馬魚基因體進行高輸出量「基因捕獵」之研究. 國立臺灣大學牙醫專業學院口腔生物科學研究所碩士論文, 2010 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56595 | - |
| dc.description.abstract | Six1同源基因對於脊椎動物的器官形成扮演著重要的角色，當老鼠缺乏Six1同源基因表現時，顱顏發育相關所衍生器官的例如骨骼肌、腎臟、甲狀腺、感覺器官與神經節等皆會產生嚴重的缺失。而人類的SIX1突變則會產生Branchio-Oto-Rena症候群 Branchio-Oto-Renal syndrome(BOR)，造成聽力喪失或是咽弓缺失。因此可得知其對於調控器官發育及細胞的分化上扮演著重要的角色。
根據前人的研究可以得知，在Six1基因的上下游有許多高度保守序列，可以調控其基因表現。包含有五段高度保守的序列(conserved sequence)，分別命名為UCR1 (upstream conserved region 1)、UCR2、UCR3、UCR4以及DCR (downstream conserved region)，其中UCR1、UCR2、UCR3以及UCR4位於six1上游，而且其中UCR1及UCR2位於斑馬魚six1近端2.6 kb的範圍內。然而，由於之前學長的研究(詳見呂智楷論文)所結合的是Six1近端130bp與280bp的啟動子片段，可能仍有其本身的促進子存在，並無法區分是否為這些高度保守片段的作用。 本實驗主要想了解這些高度保守序列是否會單獨影響Six1基因的表現，因此將斑馬魚six1基因近端111bp之啟動子片段與Tol2轉位子載體的質體結合，以顯微注射的方式送到斑馬魚胚胎中，確認此片段中不含任何促進子(enhancer)活性，GFP螢光蛋白不在任何組織表現。之後，再將UCR1、UCR2、UCR3、UCR4以及DCR2等各序列與此質體結合，以顯微注射的方式送到斑馬魚胚胎中，在此可觀察到含有各片段之結構體在體節(somite)、耳囊(otic vesicle)、神經丘(neuromast)以及咽弓區域(branchial arch)等不同的組織器官中有綠色螢光蛋白的表現，顯示這些片段所含之促進子(enhancer)，會影響Six1的基因表現。 綜合上述，本實驗於斑馬魚six1基因的上下游相關序列，發現這五個保守片段可能分別包含某些會影響Six1基因表現的促進子。 | zh_TW |
| dc.description.abstract | he Six1 homeobox gene plays a critical roles in vertebrate organogenesis. Mice deficient for Six1 show severe defects in organs such as skeletal muscle, kidney, thymus, sensory organs and ganglia derived from cranial placodes. Mutations in human SIX1 cause Branchio-Oto-Renal syndrome(BOR), characterized by hearing loss and branchial defects. Six1 genes are hypothesized to play important roles in organgenesis and cell differentiation.
Depending on the previous study, there are a lot of conserved sequences around the six1 gene downstream and upstream,including 5 conserved sequences, namly UCR1 (upstream conserved region 1), UCR2, UCR3, UCR4 and DCR (downstream conserved region), The previous study used six1 proximal 130bp and 280bp promoter fragments, which may contain enhancer elements, to delineate the UCRs and DCR activity. These fragments were inserted upstream of the zebrafish six1 proximal promoter(111bp) in Tol-2 transposon elements. The UCR1 and UCR2 are located within the six1 2.6 kb proximal region. I analyzed the highly conserved sequences UCRs and DCR in conjugation with six1 111bp proximal fragment by transgenic zebrafish assay. The resulting reporter-EGFP expression showed spatial restrictions similar to those of six1.1 mRNA detected by in situ hybridization (i.e., mainly in somites, otic vesicle and neuromasts).These results revealed enhancers which can influence six1 gene expression. Taken together, these results suggested that these conserved sequences contain enhancers which can influence six1 gene expression. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:36:47Z (GMT). No. of bitstreams: 1 ntu-103-R99450016-1.pdf: 5944172 bytes, checksum: bb216f46d71237601163137086c05a59 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 口試委員會審定書………………………………………………ii
誌謝………………………………………………………………………iii 中文摘要………………………………………………………………iv 英文摘要…………………………………………………………………v 壹、前言…………………………………………………………………1 貳、實驗材料………………………………………………………43 參、實驗方法………………………………………………………51 肆、結果………………………………………………………………58 伍、討論………………………………………………………………63 陸、圖表………………………………………………………………65 參考文獻………………………………………………………………87 | |
| dc.language.iso | zh-TW | |
| dc.subject | 耳囊 | zh_TW |
| dc.subject | Six1 | zh_TW |
| dc.subject | Tol2元素 | zh_TW |
| dc.subject | 斑馬魚 | zh_TW |
| dc.subject | 保守序列 | zh_TW |
| dc.subject | 神經丘 | zh_TW |
| dc.subject | 體節 | zh_TW |
| dc.subject | otic vesicle | en |
| dc.subject | zebrafish | en |
| dc.subject | conserved sequence | en |
| dc.subject | neuromast | en |
| dc.subject | somite | en |
| dc.subject | Six1 | en |
| dc.title | 藉由綠色螢光蛋白報導基因與Tol2跳躍基因系統研究斑馬魚Six1基因啟動子之顱顏組織專一性表現 | zh_TW |
| dc.title | Functional Analysis of Six1 Gene Enhancer Elements During Craniofacial Development by Transgenic Zebrafish with Green Fluorescent Protein (GFP) Reporter Gene and Tol2 transposon system | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 江俊斌(Chun-Ping Chiang),姚宗珍(Jane Yao) | |
| dc.subject.keyword | Six1,Tol2元素,斑馬魚,保守序列,神經丘,體節,耳囊, | zh_TW |
| dc.subject.keyword | Six1,zebrafish,conserved sequence,neuromast,somite,otic vesicle, | en |
| dc.relation.page | 91 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-13 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
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