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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳美如(Mei-Ru Chen) | |
| dc.contributor.author | I-Fang Wu | en |
| dc.contributor.author | 吳宜芳 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:16:51Z | - |
| dc.date.available | 2019-10-09 | |
| dc.date.copyright | 2014-10-09 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-17 | |
| dc.identifier.citation | 許嘉琳，(2012)EB病毒對於星狀細胞株之感染及影響，國立台灣大學醫學院微生物所微生物與免疫學組碩士論文。
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Nature reviews Cancer 4, 757-768. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56145 | - |
| dc.description.abstract | EB病毒 (Epstein–Barr virus) 是一種人類皰疹病毒，主要感染B細胞和上皮細胞。目前已知大多數的感染性單核球增多症 (Infection mononucleosis, IM) 是由EBV所引起。因EBV感染IM的部分病人會產生中樞神經系統 (Central nervous system, CNS) 發炎併發症，例如腦炎和脊髓炎，在這些CNS發炎患者的腦脊髓液中，能測到EBV基因或anti-EBV的抗體。且根據文獻，當EBV存在中樞神經系統時，可能會加重由其他病原體所造成的CNS發炎或病症。而CNS中數量最多的星狀細胞，具有維持CNS生理機能的多種功能外，亦能透過釋放細胞激素 (cytokine) 等方式調控CNS的免疫反應。已知EBV能在體外感染人類星狀細胞株，因此本研究使用本實驗室建立的兩種EB病毒陽性星狀細胞株探討EB病毒感染對星狀細胞功能的影響。先以促發炎因子IL-1β或TNF-α刺激這兩種EBV陽性星狀細胞株，再以Q- PCR測定細胞中細胞激素IL-6 與IL-8 mRNA表現量的變化。在EB病毒陽性星狀細胞株U-87E以IL-1β刺激後增加了IL-6表現量，但IL-8的表現量沒有明顯改變。為了避免實驗結果是因為混合株間特性的差異，使用其他EBV 陽性細胞株U251-E、U87-E2Pl1與U87-E2Pl2來驗證IL-1β處理後的這些細胞也有EB病毒感染增強IL-6 RNA產量的影響。因為EB病毒陽性細胞株會表現潛伏期蛋白LMP1和EBNA1，以慢病毒感染的方式讓星狀細胞株U-87MG大量表現LMP1，表現LMP1的星狀細胞株會促進IL-6與IL-8的表現，再給予IL-1β刺激後，可以觀察到LMP1的表現與IL-1β刺激兩者有加乘的效果。結果顯示LMP1在EB病毒陽性星狀細胞產生細胞激素的調控上可能扮演重要的角色，這個研究對於EB病毒如何影響CNS感染而引起的的發炎有更多的了解。 | zh_TW |
| dc.description.abstract | Epstein–Barr virus (EBV) is a human herpesvirus, and the major viral tropism is B cells and epithelial cells. Most of infectious mononucleosis (IM) is caused by EBV infection. Central nervous system (CNS) complications, such as encephalitis and myelitis occur in some IM patients. EBV genomes and anti-EBV antibodies were found in the cerebrospinal fluid (CSF) from some IM patients with CNS inflammation. According to previous studies, EBV existing in CNS most likely accentuates the illness of CNS disease or CNS inflammation. Astrocytes are the most abundant cells and have multiple physiological functions in the CNS, also can modulate immune response by secreting cytokines. EBV was known to infect human astrocyte cell lines in vitro. In this study, two EBV-positive astrocyte cell lines were established in our laboratory to characterize effects of EBV infection to astrocyte function. These two EBV-positive astrocyte cell lines were stimulated with IL-1β or TNF-α and detected for IL-6 or IL-8 mRNA expression by quantitative real-time PCR. Expression of IL-6 was enhanced with IL-1β stimulation in EBV-positive astrocyte cell lines U87-E, but no obvious change was observed in the expression of IL-8 mRNA. To avoid clonal variation, additional EBV infected pooled clones, U251-E, U87-E2Pl1 and U87-E2Pl2 were used for IL-1β treatment to demonstrate the enhancing effect of EBV infection on IL-6 mRNA production. Because EBV-positive astrocyte cell lines expressed latent protein LMP1 and EBNA1, LMP1 were overexpressed in parental astrocyte cell lines by lentiviral infection. The expression of IL-6 and IL-8 mRNA were further stimulated in LMP1 over-expressing astrocyte cell lines than control cells. Data here suggest that LMP1 may regulate the expression of IL-6 and IL-8 mRNA in astrocytes. In addition, LMP1-overexpressing parental astrocytes have synergistic effect with IL-1β in the expression of IL-6 mRNA.Data here suggest that LMP1 may play a major role in regulating cytokine production of EBV positive astrocytes. This study thus shed light on how EBV infection may affect the inflammation process of CNS infection. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:16:51Z (GMT). No. of bitstreams: 1 ntu-103-R01445132-1.pdf: 1618507 bytes, checksum: d39a76ada47232e96af34b07599a3924 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 目錄
摘要………………………………………………………………….………………...ⅱ Abstract…………………………….......…………………………………….............ⅲ 目錄……………………………….………………………………………………...…ⅴ 1. 導論 1.1. EB病毒 (Epstein-Barr virus)……………..………………………….……………1 1.2.中樞神經系統 (Central Nervous System)…………….…..........................…….…6 1.3. EB病毒與中樞神經系統發炎相關病症………………….………….…………. 9 1.4. 研究動機與目的……………………………………………………….……...….10 2. 材料與方法 2.1. 材料…………………………………………………………………………….....11 2.2. 實驗方法……...…………………………………….…………………..…….…..15 3. 結果 3.1. EB病毒感染增加IL-1β對於星狀細胞株誘發IL-6 MRNA表現量之反應，對IL-8 MRNA的表現量影響有限……………………..…..…….…………………21 3.2. EB病毒陽性星狀細胞株處於潛伏期第二型……………………...……………..22 3.3. 不同種的EB病毒陽性星狀細胞株均觀察到IL-1β誘發IL-6 mRNA 表現量 增加的現象………….………………………………………………………….…….23 3.4. EBV的病毒蛋白LMP1參與調控IL-6或IL-8的mRNA 表現量，但未發現Zta對星狀細胞中IL-6 mRNA表現量的影響……………………………………....24 3.5. EB病毒對IL-1受器第一型(IL-1R1) MRNA表現之影響…….……….………..25 3.6. EB病毒感染對於星狀細胞受TNF-Α細胞激素IL-6、IL-8表現量之影響……..26 4. 討論 4.1. EB病毒的感染影響了星狀細胞株中細胞激素IL-6的產生…………………....27 4.2. 不同星狀細胞株的背景差異對實驗之影響..………………………….…......... 27 4.3. EB病毒感染與IL-6表現量增加與中樞神經疾病之連結……………….….....27 4.4. LMP1對IL-8表現量增加對中樞神經系統之影響………..………………...…28 4.5. LMP1影響星狀細胞中IL-6和IL-8產生的可能訊息傳遞路徑…………….....29 4.6. 進一步確認EB病毒感染影響細胞激素的RNA製造及蛋白釋出………...….29 4.7. EB病毒潛伏感染星狀細胞中可能透過影響細胞激素的產量的方式，間接加重在中樞神經疾病的病程發展….………….……………………………………….30 5. 圖表………….....………………………………………..……….......31 6. 參考文獻………………...………………………………………...…43 | |
| dc.language.iso | zh-TW | |
| dc.subject | EB病毒 | zh_TW |
| dc.subject | 星狀細胞 | zh_TW |
| dc.subject | IL-6 | zh_TW |
| dc.subject | 中樞神經系統 | zh_TW |
| dc.subject | 發炎反應 | zh_TW |
| dc.subject | IL-1β | zh_TW |
| dc.subject | 細胞激素 | zh_TW |
| dc.subject | EBV | en |
| dc.subject | IL-6 | en |
| dc.subject | IL-1β | en |
| dc.subject | Cytokine | en |
| dc.subject | Inflammation | en |
| dc.subject | CNS | en |
| dc.subject | Astrocyte | en |
| dc.title | EB病毒潛伏感染對星狀細胞產生細胞激素之影響 | zh_TW |
| dc.title | Regulatory Effects of EBV Latent Infection on the Cytokine Production of Astrocytes | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 徐立中,董馨蓮,吳雪霞 | |
| dc.subject.keyword | EB病毒,星狀細胞,中樞神經系統,發炎反應,細胞激素,IL-1β,IL-6, | zh_TW |
| dc.subject.keyword | EBV,Astrocyte,CNS,Inflammation,Cytokine,IL-1β,IL-6, | en |
| dc.relation.page | 50 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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