請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56142完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林仲彥(Chung-Yen Lin) | |
| dc.contributor.author | Kuan-Ying Chen | en |
| dc.contributor.author | 陳冠螢 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:16:45Z | - |
| dc.date.available | 2016-09-03 | |
| dc.date.copyright | 2014-09-03 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-17 | |
| dc.identifier.citation | Adey, A., Morrison, H. G., Asan, Xun, X., Kitzman, J. O., Turner, E. H., Stackhouse, B., MacKenzie, A. P., Caruccio, N. C., Zhang, X., & Shendure, J. (2010) Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition. Genome Biol, 11(12): R119.
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., & Lipman, D. J. (1997) Gapped BLAST and PSI-BLAST- a new generation of protein database search programs. Nucleic Acids Res, 25(17): 3389-3402. Ashburner, M., Ball, C. A., Blake, J. A., Botstein, D., Butler, H., Cherry, J. M., Davis, A. P., Dolinski, K., Dwight, S. S., Eppig, J. T., Harris, M. A., Hill, D. P., Issel-Tarver, L., Kasarskis, A., Lewis, S., Matese, J. C., Richardson, J. E., Ringwald, M., Rubin, G. M., & Sherlock, G. (2000) Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet, 25(1): 25-29. Bairoch, A., & Apweiler, R. (2000) The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucleic Acids Res, 28(1): 45-48. Batzoglou, S. (2002) ARACHNE: A Whole-Genome Shotgun Assembler. Genome Res, 12(1): 177-189. Berger, B., Peng, J., & Singh, M. (2013) Computational solutions for omics data. Nat Rev Genet, 14(5): 333-346. Bryant, D. W., Jr., Wong, W. K., & Mockler, T. C. (2009) QSRA: a quality-value guided de novo short read assembler. BMC Bioinformatics, 10: 69. Butler, J., MacCallum, I., Kleber, M., Shlyakhter, I. A., Belmonte, M. K., Lander, E. S., Nusbaum, C., & Jaffe, D. B. (2008) ALLPATHS: de novo assembly of whole-genome shotgun microreads. Genome Res, 18(5): 810-820. Chaisson, M. J., Brinza, D., & Pevzner, P. A. (2009) De novo fragment assembly with short mate-paired reads: Does the read length matter? Genome Res, 19(2): 336-346. Chao, C. B., Chen, C. Y., Lai, Y. Y., Lin, C. S., & Huang, H. T. (2004) Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV). Dis Aquat Organ, 58: 127-142. Chevreux, B., Pfisterer, T., Drescher, B., Driesel, A. J., Muller, W. E., Wetter, T., & Suhai, S. (2004) Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs. Genome Res, 14(6): 1147-1159. Chi, S. C., Lo, C. F., Kou, G. H., Chang, P. S., Peng, S. E., & Chen, S. N. (1997) Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus akaara (Temminck & Schlegel). J. Fish Dis, 20(3): 185-193. Chou, H. Y., Hsu, C. C., & Peng, T. Y. (1998) Isolation and Characterization of a Pathogenic Iridovirus from Cultured Grouper (Epinephelus sp.) in Taiwan. Fish Pathol, 33(4): 201-206. Compeau, P. E., Pevzner, P. A., & Tesler, G. (2011) How to apply de Bruijn graphs to genome assembly. Nat Biotechnol, 29(11): 987-991. Cossins, A. R., & Crawford, D. L. (2005) Fish as models for environmental genomics. Nat Rev Genet, 6(4): 324-333. Ding, X., Lu, D. Q., Hou, Q. H., Li, S. S., Liu, X. C., Zhang, Y., & Lin, H. R. (2012) Orange-spotted grouper (Epinephelus coioides) toll-like receptor 22: molecular characterization, expression pattern and pertinent signaling pathways. Fish Shellfish Immunol, 33(3): 494-503. Dohm, J. C., Lottaz, C., Borodina, T., & Himmelbauer, H. (2007) SHARCGS, a fast and highly accurate short-read assembly algorithm for de novo genomic sequencing. Genome Res, 17(11): 1697-1706. Edwards, A. S., & Scott, J. D. (2000) A-kinase anchoring proteins- protein kinase A and beyond. Curr Opin Cell Biol, 12(2): 217-221. Gnerre, S., Maccallum, I., Przybylski, D., Ribeiro, F. J., Burton, J. N., Walker, B. J., Sharpe, T., Hall, G., Shea, T. P., Sykes, S., Berlin, A. M., Aird, D., Costello, M., Daza, R., Williams, L., Nicol, R., Gnirke, A., Nusbaum, C., Lander, E. S., & Jaffe, D. B. (2011) High-quality draft assemblies of mammalian genomes from massively parallel sequence data. Proc Natl Acad Sci U S A, 108(4): 1513-1518. Gracey, A. Y., Fraser, E. J., Li, W., Fang, Y., Taylor, R. R., Rogers, J., Brass, A., & Cossins, A. R. (2004) Coping with cold: An integrative, multitissue analysis of the transcriptome of a poikilothermic vertebrate. Proc Natl Acad Sci U S A, 101(48): 16970-16975. Gurevich, A., Saveliev, V., Vyahhi, N., & Tesler, G. (2013) QUAST: quality assessment tool for genome assemblies. Bioinformatics, 29(8): 1072-1075. Heiger, D. N., Cohen, A. S., & Karger, B. L. (1990) Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields. J. Chromatogr, 516(1): 33. Huang, X. (1999) CAP3: A DNA Sequence Assembly Program. Genome Res, 9(9): 868-877. Huang, Y., Huang, X., Yan, Y., Cai, J., Ouyang, Z., Cui, H., Wang, P., & Qin, Q. (2011) Transcriptome analysis of orange-spotted grouper (Epinephelus coioides) spleen in response to Singapore grouper iridovirus. BMC Genomics, 12: 556. Jeck, W. R., Reinhardt, J. A., Baltrus, D. A., Hickenbotham, M. T., Magrini, V., Mardis, E. R., Dangl, J. L., & Jones, C. D. (2007) Extending assembly of short DNA sequences to handle error. Bioinformatics, 23(21): 2942-2944. Kai, W., Kikuchi, K., Tohari, S., Chew, A. K., Tay, A., Fujiwara, A., Hosoya, S., Suetake, H., Naruse, K., Brenner, S., Suzuki, Y., & Venkatesh, B. (2011) Integration of the genetic map and genome assembly of fugu facilitates insights into distinct features of genome evolution in teleosts and mammals. Genome Biol Evol, 3: 424-442. Kanehisa, M., & Goto, S. (2000) KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res, 28(1): 27-30. Kanehisa, M., Goto, S., Sato, Y., Furumichi, M., & Tanabe, M. (2012) KEGG for integration and interpretation of large-scale molecular data sets. Nucleic Acids Res, 40: 109-114. Kawai, T., & Akira, S. (2008) Toll-like receptor and RIG-I-like receptor signaling. Ann N Y Acad Sci, 1143: 1-20. Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K., Doyle, M., FitzHugh, W., Funke, R., Gage, D., Harris, K., Heaford, A., Howland, J., L., K., Lehoczky, J., LeVine, R., McEwan, P., McKernan, K., Meldrim, J., Mesirov, J. P., Miranda, C., Morris, W., Naylor, J., Raymond, C., Rosetti, M., Santos, R., Sheridan, A., Sougnez, C., et al. (2001) Initial sequencing and analysis of the human genome. Nature, 409(6822): 860-921. Leggett, R. M., Clavijo, B. J., Clissold, L., Clark, M. D., & Caccamo, M. (2014) NextClip: an analysis and read preparation tool for Nextera Long Mate Pair libraries. Bioinformatics, 30(4): 566-568. Li, R., Zhu, H., Ruan, J., Qian, W., Fang, X., Shi, Z., Li, Y., Li, S., Shan, G., Kristiansen, K., Li, S., Yang, H., Wang, J., & Wang, J. (2010) De novo assembly of human genomes with massively parallel short read sequencing. Genome Res, 20(2): 265-272. Lindgreen, S. (2012) AdapterRemoval: easy cleaning of next-generation sequencing reads. BMC Res Notes, 5: 337. Margulies, M., Egholm, M., Altman, W. E., Attiya, S., Bader, J. S., Bemben, L. A., Berka, J., Braverman, M. S., Chen, Y. J., Chen, Z., Dewell, S. B., Du, L., Fierro, J. M., Gomes, X. V., Godwin, B. C., He, W., Helgesen, S., Ho, C. H., Irzyk, G. P., Jando, S. C., Alenquer, M. L., Jarvie, T. P., Jirage, K. B., Kim, J. B., Knight, J. R., Lanza, J. R., Leamon, J. H., Lefkowitz, S. M., Lei, M., Li, J., et al. (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature, 437(7057): 376-380. Maxam, A. M., & Gilbert, W. (1977) A new method for sequencing DNA. Proc Natl Acad Sci U S A, 74(2): 560-564. Melamede, R. J., & Carmel, N. Y. (1989). Automatable process for sequencing nucleotide. New York Patent No. 4,863,849. Google Patents. Miller, J. R., Koren, S., & Sutton, G. (2010) Assembly algorithms for next-generation sequencing data. Genomics, 95(6): 315-327. Moriya, Y., Itoh, M., Okuda, S., Yoshizawa, A. C., & Kanehisa, M. (2007) KAAS: an automatic genome annotation and pathway reconstruction server. Nucleic Acids Res, 35: 182-185. Paszkiewicz, K., & Studholme, D. J. (2010) De novo assembly of short sequence reads. Brief Bioinform, 11(5): 457-472. Petersen, T. N., Brunak, S., von Heijne, G., & Nielsen, H. (2011) SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods, 8(10): 785-786. Pop, M. (2009) Genome assembly reborn: recent computational challenges. Brief Bioinform, 10(4): 354-366. Pop, M., & Kosack, D. (2004) Using the TIGR assembler in shotgun sequencing projects. Methods Mol Biol, 255: 279-294. Preston, N. (1985) The combined effects of temperature and salinity on hatching success and the survival, growth, and development of the larval stages of Metapenaeus bennettae (Racek & Dall). J. Exp Mar Bio Ecol, 85(1): 57-74. Punta, M., Coggill, P. C., Eberhardt, R. Y., Mistry, J., Tate, J., Boursnell, C., Pang, N., Forslund, K., Ceric, G., Clements, J., Heger, A., Holm, L., Sonnhammer, E. L., Eddy, S. R., Bateman, A., & Finn, R. D. (2012) The Pfam protein families database. Nucleic Acids Res, 40: 290-301. Roest Crollius, H., & Weissenbach, J. (2005) Fish genomics and biology. Genome Res, 15(12): 1675-1682. Salzberg, S. L., Phillippy, A. M., Zimin, A., Puiu, D., Magoc, T., Koren, S., Treangen, T. J., Schatz, M. C., Delcher, A. L., Roberts, M., Marcais, G., Pop, M., & Yorke, J. A. (2012) GAGE: A critical evaluation of genome assemblies and assembly algorithms. Genome Res, 22(3): 557-567. Sanger, F., Nicklen, S., & Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A, 74(12): 5463-5467. Schatz, M. C., Delcher, A. L., & Salzberg, S. L. (2010) Assembly of large genomes using second-generation sequencing. Genome Res, 20(9): 1165-1173. Simpson, J. T., Wong, K., Jackman, S. D., Schein, J. E., Jones, S. J., & Birol, I. (2009) ABySS: a parallel assembler for short read sequence data. Genome Res, 19(6): 1117-1123. Sonnhammer, E. L., von Heijne, G., & Krogh, A. (1998) A hidden Markov model for predicting transmembrane helices in protein sequences. Proc Int Conf Intell Syst Mol Biol, 6: 175-182. Stanke, M., Diekhans, M., Baertsch, R., & Haussler, D. (2008) Using native and syntenically mapped cDNA alignments to improve de novo gene finding. Bioinformatics, 24(5): 637-644. Stanke, M., & Waack, S. (2003) Gene prediction with a hidden Markov model and a new intron submodel. Bioinformatics, 19(2): 215-225. Tasken, K. A., Collas, P., Kemmner, W. A., Witczak, O., Conti, M., & Tasken, K. (2001) Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area. J. Biol Chem, 276(25): 21999-22002. Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., Gocayne, J. D., Amanatides, P., Ballew, R. M., Huson, D. H., Wortman, J. R., Zhang, Q., Kodira, C. D., Zheng, X. H., Chen, L., Skupski, M., Subramanian, G., Thomas, P. D., Zhang, J., Gabor Miklos, G. L., Nelson, C., Broder, S., Clark, A. G., Nadeau, J., McKusick, V. A., Zinder, N., et al. (2001) The sequence of the human genome. Science, 291(5507): 1304-1351. Wang, M. J., Wang, L., & Zhou, Z. G. (2008) Structure and Function of A kinase anchoring proteins and Their Signaling Complexes. Chin J Biochem Mol Biol, 24(2): 101-106. Warren, R. L., Sutton, G. G., Jones, S. J., & Holt, R. A. (2007) Assembling millions of short DNA sequences using SSAKE. Bioinformatics, 23(4): 500-501. Yoshikoshi, K., & Inoue, K. (1990) Viral nervous necrosis in hatchery-reared larvae and juveniles of Japanese parrotfish, Oplegnathus fasciatus (Temminck & Schlegel). J. Fish Dis, 13(1): 69-77. Zerbino, D. R., & Birney, E. (2008) Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res, 18(5): 821-829. 于乃衡 (2008) 台灣繁養殖魚介貝類專刊XIV。中華民國水產種苗協會，6-11。 行政院農業委員會水產試驗所。http://www.tfrin.gov.tw/ct.asp?xItem=159462&ctNode=1225&mp=1 李昆霖 (2013) 以次世代定序資料重組南美白蝦的轉錄基因體並探討其基因表現。國立臺灣大學漁業科學研究所碩士論文。 林宜靜 (2012) 以表現標誌序列重組南美白蝦之轉錄基因體並比較不同組織間的基因表現。國立臺灣大學漁業科學研究所碩士論文。 施俊誠、曾美珍 (2009) 台灣七種養殖石斑魚的核型鑑定。98年度台灣水產學會暨學術論文發表會。(poster) 陳宗嶽、王廷瑜、徐浩軒 (2013) 石斑魚的恐怖病毒殺手。科學發展，482: 44-48。 黃貴民、曾金城、楊千里、張美香 (1994) 石斑魚苗養殖成本之探討。中國水產，493: 16-21。 楊玉婷、陳葦芋、陳政忻 (2009) 石斑魚產業概況及趨勢。農業生技產業季刊，19: 26-29。 劉秉忠 (2007) 石斑魚養殖要點。石斑Hand book of Grouper，台灣漁業經濟發展協會及行政院農委會，38-46。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56142 | - |
| dc.description.abstract | 本研究以現今台灣重要具高經濟價值的養殖魚種龍膽石斑（Epinephelus lanceolatus）為研究對象，利用次世代定序技術，取得短序列散彈槍式基因體定序資料，以全新組裝（de novo Assembly）的方式，得到龍膽石斑的基因體支架 (scaffold)，並由其中預測其可轉譯蛋白質序列，以作為基因體表現概況、調節機制，與免疫相關反應之功能性研究的重要基礎。
本研究比較數種不同組裝策略，其中以ALLPATHS-LG組裝軟體，加上SOAPdenovo組裝軟體裡的GapCloser程式所組裝的結果，其質與量均為最佳，可作為基因體組裝後續的分析基礎。組裝序列共有13,897條scaffolds，序列總和為1.09 Gbp。為了找出石斑魚基因體中所存在的基因，我們以AUGUSTUS演算模型，選用八目鰻 (Petromyzon marinus, 俗名：sea lamprey) 的基因模型做為參考進行預測，共可轉譯出42,433條蛋白質序列，其中92.6%的序列，能在nr蛋白質資料庫中找到對應序列（blastp, E-value：1E-5）。分析所對應序列的物種組成，有2/3以上皆屬脊索動物門輻鰭綱之物種，其餘1/3則為微生物序列，可能與樣本採集過程污染有關。將含有細菌的序列濾除後，保留9,473條scaffolds，序列長度總和為1.06 Gbp，與一般對石斑魚所估計的基因體大小 (1.1G bp) 接近；由此 9,473 條龍膽石斑的基因體支架得到的蛋白質序列為29,184條，有26,056條蛋白質(89.3 %)序列可在nr找到對應序列（blastp, E-value：1E-5），這些對應序列中有 93% 歸屬於輻鰭魚綱。與KEGG資料庫的比對後，有26,328筆對應結果，與 Pfam資料庫共有24,193條序列有蛋白質模組構造的註解，SignalP預測顯示有1,283條蛋白具有訊息胜肽，tmHMM分析顯示有5,302條預測具有穿膜蛋白構造。在比較基因體學的部分，本研究選用河魨(Fugu rubripes)、斑馬魚(Danio rerio)及吳郭魚(Oreochromis niloticus)，將所預測出的轉譯蛋白序列進行互相比對，可發現任兩物種之間的可對應序列都接近 80% 或更高。 最後我們將組裝序列與註解結果資料整合，建構成線上資料庫，呈現石斑魚最完整的基因序列目錄與相關的調控代謝網路，將在日後整合轉錄體的相關資料，可於線上即時分析差異表現基因所涉及的代謝網路與找出相關參與基因群，以深入更廣泛地方式來瞭解複雜的調控機制。本研究期望能對龍膽石斑相關研究有所助益，並能對龍膽石斑基因體更深入的瞭解，進而對龍膽石斑成長、育種與病理等基礎研究有所助益。 | zh_TW |
| dc.description.abstract | Giant grouper (Epinephelus lanceolatus) is one of the most economically valuable aquaculture species in Taiwan. Its genome is estimated in 1.1G and has not been fully sequenced. In this study, we apply next-generation sequencing (NGS) technology to obtain whole genome shotgun sequences, then to do de novo assembly for recover the genome of E. lanceolatus from short reads. Then we annotate the genome of E. lanceolatus. Four different assembly strategies are evaluated for the best one to conclude genome scaffold. We chose ALLPATHS-LG as the assembler and used GapCloser to further fill the intra-scaffold gaps. Total 13,897 scaffolds, in total length sum of 1.09 G bp, were derived, from which 42,433 putative protein coding genes were predicted by AUGUSTUS using gene model of sea lamprey (Petromyzon marinus). Overall 92.6% of the protein products were annotated by nr (blastp, E-value: 1E-5). Among these best matched nr sequences, more than two-thirds protein coding sequences are from Phylum Actinopterygii, and the other one-third match to bacterial proteins. It indicate a possible source of sample contamination. We further remove scaffolds of bacterial origin and the final set is 9,473 scaffolds, sum up to 1.06 G bp; 29,184 protein sequences were derived and 89.3% of the best matched sequences were from Phylum Actinopterygii. There are 26,328 protein sequences mapped on KEGG database. We further annotated these protein sequences using Pfam, SignalP and tmHMM to reveal protein structure information. We use the whole set of protein coding genes derived from E. lanceolatus, Fugu rubripes, Danio rerio and Oreochromis niloticus and find the best hits for each proteins mutually by blast. About 80% of the protein sequences can find match between any two species.
Finally, we integrated the protein coding sequences with their annotations into a web database. This database will be open and become a helpful grouper genome resource for research community. It will benefit the grouper researchers for studying physiology and pathology, as well as for defining genetic traits such as fast growing rate and disease resistance for breeding. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:16:45Z (GMT). No. of bitstreams: 1 ntu-103-R98b45007-1.pdf: 5867506 bytes, checksum: dd732405a65bb9f8a8e76d295fda0869 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iv 目錄 vi 圖目錄 viii 表目錄 x 第一章 簡介 1 1.1 研究背景 1 1.1.1 龍膽石斑養殖 1 1.1.2 NGS發展與優勢 5 1.1.3 組裝原理 6 1.1.4 石斑魚基因體 11 1.2 研究動機與目的 12 第二章 材料與方法 14 2.1 龍膽石斑的基因體定序資料 14 2.2 組裝流程 14 2.2.1 序列資料前處理 15 2.2.2 De novo Assembly 16 2.2.3 組裝品質評估 20 2.3 基因結構預測 21 2.4 組裝序列進行蛋白質功能註解 21 2.4.1 尋找組裝序列之同源序列 22 2.4.2 組裝序列蛋白質功能模組註解 22 2.4.3 以GO資料庫對組裝序列進行註解 22 2.4.4 組裝序列在代謝途徑的對應位置 23 2.5 基因體比較 23 2.6 硬體資訊及建置龍膽石斑魚基因體資料庫 24 2.7 資料庫與程式清單 25 第三章 結果與討論 27 3.1 資料前處理結果 27 3.2 定序及組裝結果與比較 30 3.3 基因結構預測結果 37 3.4 對組裝序列進行功能性註解 40 3.4.1 同源序列比對結果 40 3.4.2 蛋白質功能模組註解結果 44 3.4.3 以GO資料庫註解結果 44 3.4.4 代謝途徑的對應位置結果 47 3.4.5 免疫調節與生長代謝相關基因分析 49 3.5 基因體比較結果 55 3.6 線上資料整合與呈現 58 第四章 結論 60 參考文獻 62 | |
| dc.language.iso | zh-TW | |
| dc.subject | 基因體定序 | zh_TW |
| dc.subject | 次世代定序 | zh_TW |
| dc.subject | 註解 | zh_TW |
| dc.subject | 龍膽石斑 | zh_TW |
| dc.subject | 組裝 | zh_TW |
| dc.subject | next-generation sequencing | en |
| dc.subject | Epinephelus lanceolatus | en |
| dc.subject | annotation | en |
| dc.subject | de novo assembly | en |
| dc.subject | genome sequencing | en |
| dc.title | 龍膽石斑之全基因體定序組裝與功能註解分析 | zh_TW |
| dc.title | Whole Genome Assembly and Annotation of the Giant Grouper, Epinephelus lanceolatus in Next Generation Sequencing | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳宗嶽(Tsung-Yueh Chen),韓玉山(Yu-Shan Han),陳淑華(Shu-Hua Chen) | |
| dc.subject.keyword | 龍膽石斑,次世代定序,基因體定序,組裝,註解, | zh_TW |
| dc.subject.keyword | Epinephelus lanceolatus,next-generation sequencing,genome sequencing,de novo assembly,annotation, | en |
| dc.relation.page | 69 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-18 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-103-1.pdf 未授權公開取用 | 5.73 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。