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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳秀男(Shiu-Nan Chen) | |
| dc.contributor.author | Min-Feng Yang | en |
| dc.contributor.author | 楊珉楓 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:08:53Z | - |
| dc.date.available | 2024-08-19 | |
| dc.date.copyright | 2014-08-25 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-19 | |
| dc.identifier.citation | 中華民國台灣地區漁業統計年報，1987-2013。行政院農業委員會漁業署。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55814 | - |
| dc.description.abstract | 本論文研究目的在於利用不同濃度的菇蕈多醣體飼料，經由投餵的方式給予實驗白蝦並檢測白蝦其總血球數 (THC)、原酚氧化酵素活性 (proPO)、超氧陰離子的產生率(O2-)、吞噬率 (PR)、吞噬指數 (PI)，以探討菇蕈多醣體給予對於白蝦先天性免疫反應的影響，同時在不同時間點予以病原體，觀察白蝦免疫力指標與抗溶藻弧菌能力的關係和存活率，以分析出適合的劑量及投餵頻率供現場養殖業者作為參考。結果顯示如下：
第一部分-菇蕈多醣體對白蝦免疫力的影響，三種不同的多醣濃度(1、2、4 g / kg diet) 對總血球數 (THC)、原酚氧化酵素活性 (proPO)、超氧陰離子的產生率(O2-)、吞噬率 (PR)、吞噬指數 (PI)等免疫指標均有顯著提升 (p<0.05) 的效果。尤其以高劑量組(4 g / kg diet)能最快提升免疫指標(THC、proPO、PR、PI)，低劑量組(1 g / kg diet)需要較長的時間提升免疫力。 第二部分-菇蕈多醣體對白蝦感染溶藻弧菌後其免疫力和存活率的影響，實驗結果顯示，白蝦在接受溶藻弧菌試驗後，與Blank組(僅注射PBS未含細菌)相比，免疫指標均有明顯下降，高劑量組(4 g / kg diet)在溶藻弧菌感染實驗期間，能使proPO活性、O2-產生率、PR、PI、THC在短時間內提升，且達到最高。在抗菌能力方面，每組連續投餵後第0、1、3、5、7、10天進行溶藻弧菌攻毒(〖4.5×10〗^5 cfu shrimp-1)，其48 h之存活率為40、80、70、60、70、80 %。低劑量組(1 g / kg diet)需要較長的時間提升免疫力，在抗菌能力方面，每組連續投餵後第0、1、3、5、7、10天進行溶藻弧菌攻毒(〖4.5×10〗^5 cfu shrimp-1)，其48 h之存活率為40、70、50、50、60、60 %。Control組存活率為40、40、40、50、50、40 %。 綜合本研究結果發現，飼料中添加菇蕈多醣體，可有效提升白蝦先天性免疫反應與抗溶藻弧菌能力。 | zh_TW |
| dc.description.abstract | The purpose of this study is using feed with different concentrations of β-glucan on white shrimp, and examine the variance of total haemocyte count (THC), prophenoloxidase (proPO), superoxide anion (O2-), phagocytic rate (PR), phagocytic index (PI). It is investigate the effect of β-glucan on innate immune response of white shrimp and observed the immune parameter, survival rate of white shrimp which injected the pathogens at different fed time points. It can be analyze the appropriate dosage and frequency of feeding for the aquaculture industry.
Section I, different dietary doses (1、2、4 g / kg diet) of β-glucan were used. The result showed that the THC, proPO, O2- production ratio, PR and PI were higher significantly (P<0.05) than the control group. The high-dose group (4 g / kg diet) can enhance the immune parameters fastest (THC, proPO, PR, PI). The low-dose group (1 g / kg diet) take a more time to increase immune parameters. Section II, white shrimp after injected the Vibrio alginolyticus compared with the Blank group, the immune parameters were significantly reduced. The high-dose group (4 g / kg diet) can enhance the immune parameters fastest, and the survival rate of white shrimp which injected V. alginolyticus after 48h at different fed time points is 40、80、70、60、70、80 % (day 0、1、3、5、7、10). The low-dose group (1 g / kg diet) take a more time to increase immune parameters, and the survival rate of white shrimp which injected Vibrio alginolyticus after 48h at different fed time points is 40、70、50、50、60、60 % (day 0、1、3、5、7、10). These results indicate β-glucan can effectively enhance the innate immune response and the resistance against Vibrio alginolyticus of white shrimp. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:08:53Z (GMT). No. of bitstreams: 1 ntu-103-R01b45015-1.pdf: 1444035 bytes, checksum: 60cd71a421445ccc35c5191f08f6a1d9 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 謝辭 i
中文摘要 ii Abstract iii 目錄 iv 表目錄 v 圖目錄 vi 第一章 前言 1 第二章 文獻整理 3 第三章 材料與方法 16 第四章 結果 20 第五章 討論 25 第六章 結論 34 第七章 參考文獻 35 表 50 圖 51 附錄 一、 藥品配置 66 | |
| dc.language.iso | zh-TW | |
| dc.subject | 菇蕈多醣體 | zh_TW |
| dc.subject | 溶藻弧菌 | zh_TW |
| dc.subject | 免疫指標 | zh_TW |
| dc.subject | 抗菌能力實驗 | zh_TW |
| dc.subject | 白蝦 | zh_TW |
| dc.subject | β-glucan | en |
| dc.subject | Challenge test | en |
| dc.subject | Litopenaeus vannamei | en |
| dc.subject | Vibrio alginolyticus | en |
| dc.subject | Immune parameters | en |
| dc.title | 菇蕈多醣體對白蝦先天性免疫能力的影響以及抵抗溶藻弧菌的能力 | zh_TW |
| dc.title | The effect of β-glucan on innate immune responses of Litopenaeus vannamei and its resistance against Vibrio alginolyticus infection | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 冉繁華(Fan-Hua Nan),劉秉忠(Ping-Chung Liu),黃世鈴(Shin-Ling Huang),陳哲俊(Che-Chun Chen),古鎮鈞(Chen-Chun Ku) | |
| dc.subject.keyword | 白蝦,菇蕈多醣體,免疫指標,溶藻弧菌,抗菌能力實驗, | zh_TW |
| dc.subject.keyword | Litopenaeus vannamei,β-glucan,Immune parameters,Vibrio alginolyticus,Challenge test, | en |
| dc.relation.page | 67 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-19 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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