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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 丁詩同(Shih-Tong Ding) | |
dc.contributor.author | Shih-Yu Fan | en |
dc.contributor.author | 范詩宇 | zh_TW |
dc.date.accessioned | 2021-06-16T05:07:57Z | - |
dc.date.available | 2015-08-25 | |
dc.date.copyright | 2014-08-25 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-19 | |
dc.identifier.citation | 林欣瑜。2011。口服二十二碳六烯酸與離胺酸改善小鼠脂肪肝現象。碩士論文。國立臺灣大學。台北市。
冒國祥、李亮、蘇衍菁、張雪、龔道清。2006。不同色素添加劑對蛋黃著色效果的影響。中國家禽28(24):140-142。 倪燕君。1997。脂肪肝的發病機理和診斷治療研究進展。國外醫學-消化系疾病分冊。17(3):158-162。 陳思孝。2009。口服蘇丹紅IV於產蛋褐色菜鴨體內之分佈。碩士論文。國立中興大學。台中市。 張其胜、袁孟彪。1993。脂肪肝的診斷進展。實用內科雜誌。13(1):39-39。 劉鈺亭。2009。散血草降低四氯化碳誘導小鼠肝臟纖維化。碩士論文。中國醫藥大學。台中市。 Abplanalp, H., and D. Napolitano. 1987. Genetic predisposition for fatty liver ruptures in White Leghorn hens of a highly inbred line. Poultry Science 66: 52. Annison, E. 1983. Lipid metabolism. In: Freeman (Ed), Physiology and Biochemistry of the Domestic Fowl. Academic Press 4: 165-174. Back, D., M. Goldman, J. Fisch, R. Ochs, and A. Goodridge. 1986. The fatty acid synthase gene in avian liver. Two mRNAs are expressed and regulated in parallel by feeding, primarily at the level of transcription. Journal of Biological Chemistry 261: 4190-4197. Branton, S., B. Lott, W. Maslin, and E. Day. 1995. Fatty liver-hemorrhagic syndrome observed in commercial layers fed diets containing chelated minerals. Avian Diseases: 631-635. Bryden, W., R. Cumming, and D. Balnave. 1979. The influence of vitamin A status on the response of chickens to aflatoxin B1 and changes in liver lipid metabolism associated with aflatoxicosis. British Journal of Nutrition 41: 529-540. Butler, E. J. 1975. Lipid metabolism in the flow under normal and abnormal circumstances. Proceedings of the Nutrition Society. 34: 29-34. Butler, E. 1976. Fatty liver diseases in the domestic fowl—A review. Avian Pathology 5: 1-14. Chand, N., F. R. Durrani, S. Ahmad, and A. Khan. 2011. Immunomodulatory and hepatoprotective role of feed‐added Berberis lycium in broiler chicks. Journal of the Science of Food and Agriculture 91: 1737-1745. Cherian, G., and M. P. Goeger. 2004. Hepatic lipid characteristics and histopathology of laying hens fed CLA or n− 3 fatty acids. Lipids 39: 31-36. Clarke, S. D. 2001. Polyunsaturated fatty acid regulation of gene transcription: a molecular mechanism to improve the metabolic syndrome. The Journal of Nutrition 131: 1129-1132. Clarke, S. D., M. K. Armstrong, and D. B. Jump. 1990. Dietary polyunsaturated fats uniquely suppress rat liver fatty acid synthase and S14 mRNA content. The Journal of Nutrition 120: 225-231. Cohen, Z. 1994. Production potential of eicosapentaenoic acid by Monodus subterraneus. Journal of the American Oil Chemists’ Society 71: 941-945. Cornelius, C. E. 1989. Liver function. KANEKO, JJ Clinical Biochemistry of Domestic Animals 4: 364-397. Couch, J. 1956. Fatty livers in laying hens: a condition which may occur as a result of increased strain. Feedstuffs 28: 46-51. David, F., P. Sandra, and A. K. Vickers. 2005. Column selection for the analysis of fatty acid methyl esters. Food analysis application. Palo Alto, CA: Agilent Technologies. Dinarello, C. A. 2000. Proinflammatory cytokines. Chest Journal 118: 503-508. Dixon, J. B., P. S. Bhathal, and P. E. O'Brien. 2001. Nonalcoholic fatty liver disease: predictors of nonalcoholic steatohepatitis and liver fibrosis in the severely obese. Gastroenterology 121: 91-100. Dugaiczyk, A., A. S. Inglis, P. M. Strike, R. W. Burley, W. G. Beattie, and L. Chan. 1981. Comparison of the nucleotide sequence of cloned DNA coding for an apolipoprotein (apoVLDL-II) from avian blood and the amino acid sequence of an egg-yolk protein (apovitellenin I): equivalence of the two sequences. Gene 14:175-182. Duplus, E., M. Glorian, and C. Forest. 2000. Fatty acid regulation of gene transcription. Journal of Biological Chemistry 275: 30749-30752. Etches, R., and F. Cunningham. 1976. The interrelationship between progesterone and luteinizing hormone during the ovulation cycle of the hen (Gallus domesticus). Journal of Endocrinology 71: 51-58. Friedman, S. L. 2000. Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury. Journal of Biological Chemistry 275: 2247-2250. Giannini, E., F. Botta, A. Fasoli, P. Ceppa, D. Risso, P. B. Lantieri, G. Celle, and R. Testa. 1999. Progressive liver functional impairment is associated with an increase in AST/ALT ratio. Digestive Diseases and Sciences 44: 1249-1253. Giovanna, P. R., D. Oliveira, and D. B. Rodriguez‐Amaya. 2007. Processed and prepared corn products as sources of lutein and zeaxanthin: Compositional variation in the food chain. Journal of Food Science 72: S079-S085. Griffin, H., and D. Hermier. 1988. Plasma lipoprotein metabolism and fattening in poultry. In : Leanness in domestic bird, genetic, metabolic and hormonal aspects. Leclercq, B. and C. C. Whitehead, eds. pp: 175-201. Gu, S., P. Wang, and R. Jiang. 2007. A study on the preventive effect of Lycium barbarum polysaccharide on the development of alcoholic fatty liver in rats and its possible mechanisms. Chinese Journal of Hepatology 15: 204-208. Guo, X. Q., H. B. Cao, G. L. Hu, C. Y. Zhang, H. T. Li, H. F. Chao, A. M. Huang, J. R. Luo, and L. Li. 2012. Effect of high-energy low-protein diet supplemented with biotin on fat metabolism of laying hens. Chinese Journal of Veterinary Science 5: 25. Hansen, R., and R. Walzem. 1993. Avian fatty liver hemorrhagic syndrome: a comparative review. Advances in Veterinary Science and Comparative Medicine 37: 451. Haugh, R. R. 1937. The Haugh Unit for measuring egg quality. U.S. Egg Poultry Magazine. 43: 552-555, 572-573. Hermier, D. 1997. Lipoprotein metabolism and fattening in poultry. The Journal of Nutrition 127: 805S-808S. Huang, Y., J. Lu, and Y. Shen. 1999. The protective effects of total flavonoids from Lycium barbarum L. on lipid peroxidation of liver mitochondria and red blood cell in rats. Journal of Hygiene Research 28: 115-116. Hurwitz, S., A. Bar, M. Katz, D. Sklan, and P. Budowski. 1973. Absorption and secretion of fatty acids and bile acids in the intestine of the laying fowl. The Journal of Nutrition 103: 543-547. Ivy, C. and M. Nesheim. 1973. Factors influencing the liver fat content of laying hens. Poultry Science 52: 281-291. Jaussi, A. W., W. S. Newcomer, and H. H. Thayer. 1962. Hyperlipemic effect of ACTH injection in the chick. Poultry Science 41: 528-532. Jensen, L., J. Casey, S. Savage, and W. Britton. 1976. An association of hardness of water with incidence of fatty liver syndrome in laying hens. Poultry Science 55: 719-724. Jia, Y., J. Dong, X. Wu, T. Ma, and A. Shi. 1998. The effect of Lycium barbarum polysaccharide on vascular tension in two-kidney, one clip model of hypertension. Acta Physiologica Sinica 50: 309-314. Kamradt, T. and A. Mitchison. 1997. Triggering the immune response by proinflammatory cytokines. Journal of Biosciences 22: 399-405. Kathirvel, E., K. Morgan, G. Nandgiri, B. C. Sandoval, M. A. Caudill, T. Bottiglieri, S. W. French, and T. R. Morgan, 2010. Betaine improves nonalcoholic fatty liver and associated hepatic insulin resistance: a potential mechanism for hepatoprotection by betaine. American journal of physiology. Gastrointestinal and Liver Physiology 299: G1068-1077. Kobatake, Y., K. Kuroda, H. Jinnouchi, E. Nishide, and S. Innami. 1984. Differential effects of dietary eicosapentaenoic and docosahexaenoic fatty acids on lowering of triglyceride and cholesterol levels in the serum of rats on hypercholesterolemic diet. Journal of Nutritional Science and Vitaminology 30: 357-372. Krogdahl, A. 1985. Digestion and absorption of lipids in poultry. The Journal of Nutrition 115: 675-685. Kralik, G., Z. Škrtić, P. Suchy, E. Strakova, and Z. Gajčević. 2008. Feeding oil and linseed oil to laying hens to increase the n-3 PUFA in egg yolk. Acta Veterinaria 77: 561-568. Kyle, D., E. Schaefer, G. Patton, and A. Beiser. 1999. Low serum docosahexaenoic acid is a significant risk factor for Alzheimer’s dementia. Lipids 34: S245-S245. Leveille, G. A. 1969. In vitro hepatic lipogenesis in the hen and chick. Comparative Biochemistry and Physiology 28: 431-435. Lim, T. K. 2013. Lycium barbarum. Edible Medicinal and Non-Medicinal Plants 6: 240-266. Linder, M. C. 1985. Nutrition and Metabolism of Fats. Nutritional Biochemistry and Metabolism with Clinical Applications 2:38-39. Liu, B. H., Y. C. Wang, C. F. Kuo, W. M. Cheng, T. F. Shen, and S. T. Ding. 2005. The effects of docosahexaenoic acid oil and soybean oil on the expression of lipid metabolism related mRNA in pigs. Asian Australasian Journal of Animal Sciences 18: 1451. Lu, Y., and A. I. Cederbaum. 2008. CYP2E1 and oxidative liver injury by alcohol. Free Radical Biology and Medicine 44: 723-738. Luo, Q., Y. Cai, J. Yan, M. Sun, and H. Corke. 2004. Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Life Sciences 76: 137-149. Mangano, P., D. Janero, and M. D. Lane. 1982. Association and assembly of triglyceride and phospholipid with glycosylated and unglycosylated apoproteins of very low density lipoprotein in the intact liver cell. Journal of Biological Chemistry 257: 11463-11467. Marchesini, G., E. Bugianesi, G. Forlani, F. Cerrelli, M. Lenzi, R. Manini, S. Natale, E. Vanni, N. Villanova, N. Melchionda. 2003. Nonalcoholic fatty liver, steatohepatitis, and the metabolic syndrome. Hepatology 37: 917-923. Marra, F., A. Gastaldelli, G. Svegliati Baroni, G. Tell, and C. Tiribelli. 2008. Molecular basis and mechanisms of progression of non-alcoholic steatohepatitis. Trends in Molecular Medicine 14: 72-81. Martland, M., E. Butler, and G. Fenwick. 1984. Rapeseed induced liver haemorrhage, reticulolysis and biochemical changes in laying hens: the effects of feeding high and low glucosinolate meals. Research in Veterinary Science 36: 298-309. Mashaly, M. M., G. L. Hendricks, M.A. Kalama, A. E. Gehad, A. O. Abbas, and P. H. Patterson. 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens. Poultry Science 83: 889-894. Maxwell, M., and G. Robertson. 1998. The avian heterophil leucocyte: a review. World's Poultry Science Journal 54: 155-178. Ming, M., L. Guanhua, Y. Zhanhai, C. Guang, and Z. Xuan. 2009. Effect of the Lycium barbarum polysaccharides administration on blood lipid metabolism and oxidative stress of mice fed high-fat diet in vivo. Food Chemistry 113: 872-877. Mozaffarieh, M., S. Sacu, and A. Wedrich. 2003. The role of the carotenoids, lutein and zeaxanthin, in protecting against age-related macular degeneration: a review based on controversial evidence. Nutrition Journal 2: 20. Nagalaksbmi, D., and S. V. Rama Rao. 2000. Fatty liver hemorrhagic syndrome in laying hen. Poultry International. Najib, H., and Y. M. Al-Yousef. 2010. Essential fatty acid content of eggs and performance of Layer Hens fed with different levels of full-fat flaxseed. Journal of Cell and Animal Biology 4: 058-063. Newman, R. E., W. L. Bryden, E. Fleck, J. R. Ashes, W. A. Buttemer, L. H. Storlien, and J. A. Downing. 2002. Dietary n-3 and n-6 fatty acids alter avian metabolism: metabolism and abdominal fat deposition. British Journal of Nutrition 88: 11-18. Noyan, A., W. Lossow, N. Brot, and I. Chaikoff. 1964. Pathway and form of absorption of palmitic acid in the chicken. Journal of Lipid Research 5: 538-541. Osborne, T. F. 2000. Sterol regulatory element-binding proteins (SREBPs): key regulators of nutritional homeostasis and insulin action. Journal of Biological Chemistry 275: 32379-32382. Peng, Y., C. Ma, Y. Li, K. S. Y. Leung, Z. H. Jiang, and Z. Zhao. Quantification of zeaxanthin dipalmitate and total carotenoids in Lycium fruits (Fructus Lycii). Plant Foods for Human Nutrition 60: 161-164. Petta, S., C. Muratore, and A. Craxi. 2009. Non-alcoholic fatty liver disease pathogenesis: the present and the future. Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 41: 615-625. Polin, D., and J. H. Wolford. 1977. Role of Estrogen as a Cause of Fatty Liver Hemorrhagic Syndrome. The Journal of Nutrition 107: 873-886. Roland, D., M. Farmer, and D. Marple. 1985. Calcium and its relationship to excess feed consumption, body weight, egg size, fat deposition, shell quality, and fatty liver hemorrhagic syndrome. Poultry Science 64: 2341-2350. Safawo, T., N. Senthil, M. Raveendran, S. Vellaikumar, K. N. Ganesan, G. Nallathambi, S. Saranya, V. G. Shobhana, B. Abirami, and E. V. Gowri. 2010. Exploitation of natural variability in maize for β-carotene content using HPLC and gene specific markers. Electronic Journal of Plant Breeding 1: 548-555. Schumann, B., E. Squires, and S. Leeson. 2000. Effect of dietary flaxseed, flax oil and n-3 fatty acid supplement on hepatic and plasma characteristics relevant to fatty liver haemorrhagic syndrome in laying hens. British Poultry Science 41: 465-472. Šefer, D., A. Andonov, S. Šobajić, R. Marković, S. Radulović, D. Jakić-Dimić, and B. Petrujkić. 2011. Effects of feeding laying hens diets supplemented with omega 3 fatty acids on the egg fatty acid profile. Biotechnology in Animal Husbandry 27: 679-686. Sekimoto, K., K. Imai, Y. Kato, and H. Takikawa. 1990. Acute decrease in vitellogenin synthesis by deprivation of food and water in laying hens. Endocrinologia Japonica 37: 319-330. Semenkovich, C. F. 1997. Regulation of fatty acid synthase (FAS). Progress in Lipid Research 36: 43-53. Shimano, H., N. Yahagi, M. Amemiya-Kudo, A. H. Hasty, J. I. Osuga, Y. Tamura, F. Shionoiri, Y. Iizuka, K. Ohashi, and K. Harada. 1999. Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes. Journal of Biological Chemistry 274: 35832-35839. Shimano, H., N. Yahagi, M. Amemiya-Kudo, A. H. Hasty, J. I. Osuga, Y. Tamura, F. Shionoiri, Y. Iizuka, K. Ohashi, and K. Harada. 1999. Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes. Journal of Biological Chemistry 274: 35832-35839. Shini, S., and W. L. Bryden. 2009. Occurrence and control of fatty liver haemorrhagic syndrome (FLHS) in caged hens. Australian Egg Corporation Limited. Shin, H. D., J. H. Suh, J. H. Kim, H. Y. Lee, H. Y. Eom, U. Y. Kim, D. H. Yang, S. B. Han, and J. R. Youm. 2012. Determination of betaine in Fructus Lycii using hydrophilic interaction liquid chromatography with evaporative light scattering detection. Bulletin Korean Chemical Society 33: 553-558. Shini, S., D. Stewart, A. Shini, and W. L. Bryden. 2007. Fatty liver haemorrhagic syndrome: a condition of caged laying hens. Proceedings of the Queens Symposium Poultry Science 13: 94-98. Singh, M. 2005. Essential fatty acids, DHA and human brain. The Indian Journal of Pediatrics 72: 239-242. Sklan, D., A. Geva, P. Budowski, and S. Hurwitz. 1984. Intestinal absorption and plasma transport of lipids in chicks and rats. Comparative Biochemistry and Physiology Part A: Physiology 78: 507-510. Song, Z., I. Deaciuc, Z. Zhou, M. Song, T. Chen, D. Hill, and C. J. McClain. 2007. Involvement of AMP-activated protein kinase in beneficial effects of betaine on high-sucrose diet-induced hepatic steatosis. American Journal of Physiology - Gastrointestinal and Liver Physiology 293: G894-G902. Squires, E., and S. Leeson. 1988. Aetiology of fatty liver syndrome in laying hens. British Veterinary Journal 144: 602-609. Sun, C., Z.-w. Wei, and Y. Li. 2011. DHA regulates lipogenesis and lipolysis genes in mice adipose and liver. Molecular Biology Reports 38: 731-737. Suzuki, H., Y. Morikawa, and H. Takahashi. 2001. Effect of DHA oil supplementation on intelligence and visual acuity in the elderly. World Review of Nutrition and Dietetics 86:68-71. Tanaka, K. 1982. Nutritional factors affecting fatty acid biosynthesis in the chicken. A review. Journal of Poultry Science. 19: 65-75. Tanaka, K., S. Ohtani, and K. Shigeno. 1983. Effect of increasing dietary energy on hepatic lipogenesis in growing chicks. I. Increasing energy by carbohydrate supplementation. Poultry Science 62: 445-451. Trott, K., F. Giannitti, G. Rimoldi, A. Hill, L. Woods, B. Barr, M. Anderson, and A. Mete. 2013. Fatty Liver Hemorrhagic Syndrome in the Backyard Chicken A Retrospective Histopathologic Case Series. Veterinary Pathology 51(4): 787-795. Van Eck, J., F. G. Davelaar, T. A. M. Van Den Heuvel‐Plesman, N. Van Kol, B. Kouwenhoven, F. H. M. Guldie. 1976. Dropped egg production, soft shelled and shell‐less eggs associated with appearance of precipitins to adenovirus in flocks of laying fowls. Avian Pathology 5: 261-272. Walzem, R. L., R. J. Hansen, D. L. Williams, and R. L. Hamilton. 1999. Estrogen Induction of VLDLy Assembly in Egg-Laying Hens. The Journal of Nutrition 129: 467. Wang, Z., T. Yao, M. Pini, Z. Zhou, G. Fantuzzi, and Z. Song. 2010. Betaine improved adipose tissue function in mice fed a high-fat diet: a mechanism for hepatoprotective effect of betaine in nonalcoholic fatty liver disease. American Journal of Physiology- Gastrointestinal and Liver Physiology 298: G634-G642. Ward, O. 1994. Production of docosahexaenoic acid by Thraustochytrium roseum. Journal of Industrial Microbiology 13: 238-241. Whelan, J. 1996. Antagonistic effects of dietary arachidonic acid and n-3 polyunsaturated fatty acids. The Journal of Nutrition 126: 1086S-1091S. Wilson, S. C. and P. Sharp. 1973. Variations in plasma LH levels during the ovulatory cycle of the hen, Gallus domesticus. Journal of Reproduction and Fertility 35: 561-564. Wolford, J. and D. Polin. 1972. Lipid accumulation and hemorrhage in livers of laying chickens. A study on fatty liver-hemorrhagic syndrome (FLHS). Poultry Science 51: 1707-1713. Wu, H. T., X. J. He, Y. K. Hong, T. Ma, Y. P. Xu, and H. H. Li. 2010. Chemical characterization of Lycium barbarum polysaccharides and its inhibition against liver oxidative injury of high-fat mice. International Journal of Biological Macromolecules 46: 540-543. Xiao, J., E. C. Liong, Y. P. Ching, R. C. Chang, M. L. Fung, A. M. Xu, K. F. So, and G. L. Tipoe. 2013. Lycium barbarum polysaccharides protect rat liver from non-alcoholic steatohepatitis-induced injury. Nutrition and Diabetes 3: 81. Xin, L. 1993. Expperimental studies on early diagnosis indices of fatty liver syndrome (FLS) in laying hens. Acta Agriculturae Universitatis Jiangxiensis 1: 9. Xu, J., M. Teran-Garcia, J. H. Park, M. T. Nakamura, and S. D. Clarke. 2001. Polyunsaturated fatty acids suppress hepatic sterol regulatory element-binding protein-1 expression by accelerating transcript decay. Journal of Biological Chemistry 276: 9800-9807. Xu, M., H. Zhang, and Y. Wang. 2002. The protective effects of Lycium barbarum polysaccharide on alloxan-induced isolated islet cells damage in rats. Journal of Chinese Medicinal Materials 25: 649-651. Zhang, J., D. Chen, and B. Yu. 2008. Effect of different dietary energy sources on induction of fatty liver-hemorrhagic syndrome in laying hens. International Journal of Poultry Science 7: 1232-1236. Ziboh, V. A., K. A. Cohen, C. N. Ellis, C. Miller, T. A. Hamilton, K. Kragballe, C. R. Hydrick and J. J. Voorhees. 1986. Effects of dietary supplementation of fish oil on neutrophil and epidermal fatty acids: Modulation of clinical course of psoriatic subjects. Archives of Dermatology 122: 1277-1282. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55744 | - |
dc.description.abstract | 出血性脂肪肝 (fatty liver hemorrhagic syndrome, FLHS)為產蛋禽常見之代謝疾病。由於蛋雞進入產蛋高峰時,肝臟常因進行大量的脂質合成而產生脂肪肝之症狀,而隨著脂肪堆積的增加,造成肝臟發炎及破損而出現肝臟出血的現象,嚴重者會造成產蛋驟降並雞隻死亡,造成農民之經濟損失。本試驗之目的乃希望利用飼糧中添加二十二碳六烯酸 (docosahexaenoic acid, DHA)與枸杞,改善產蛋雞出血性脂肪肝,並生產機能蛋,增加雞蛋附加價值。
本試驗以DHA藻油 (0, 1%)及枸杞 (0, 2%)進行複因子之試驗,各處理分別為DHA藻油0%和枸杞0% (對照組)、DHA藻油0%和枸杞2%、DHA藻油1%和枸杞0% 以及DHA藻油1%和枸杞2% 之四個處理組。以84隻海蘭品系W-36來航蛋雞(36週齡),進行24週試驗 (36-60週齡),試驗期間飲水、飼料任飼。結果顯示,DHA藻油及枸杞處理不影響各處理組生長性狀及產蛋率 (P > 0.05);各處理組血漿中總膽固醇 (total cholesterol, TC)及三酸甘油酯 (triglyceride, TG)濃度無顯著差異 (P > 0.05)。而在肝臟分析方面,與對照組相較,DHA藻油可改善60週齡蛋雞FLHS出血評分、降低肝臟中TC、TG濃度及脂肪堆積 (P< 0.05);枸杞可降低60週齡蛋雞肝臟TC含量 (P< 0.05),而於36及48週齡,各處理間無顯著差異 (P > 0.05)。基因表現分析方面,DHA藻油可降低48-60週齡蛋雞acyl-CoA oxidase (ACC)及fatty acid synthase (FAS) mRNA表現量 (P < 0.05),似乎可以解釋DHA減緩脂質合成的原因。而各處理組於促發炎細胞激素tumor necrosis factor alpha (TNF-α) 與interleukin-6 (IL-6)之mRNA表現量無顯著差異 (P > 0.05),但於肝臟切片H&E染色上可觀察到有發炎反應的發生。在蛋品分析方面,DHA藻油及枸杞處理之豪氏單位無顯著差異 (P > 0.05),顯示處理對蛋的品質無負面影響;而在蛋黃色澤上,枸杞可增加蛋黃之紅色 (a值) (P < 0.05)及黃色度 (b值) (P < 0.05),而亮度 (L值)則稍為下降 (P < 0.05)。DHA則不影響蛋黃色澤 (P > 0.05),但可增加蛋中DHA含量。 綜上所示,DHA 藻油及枸杞對蛋雞生長性狀及產蛋性狀無負面之影響,而DHA 藻油可降低60週齡產蛋雞肝臟出血、肝臟中脂肪的堆積,並減緩肝臟發炎而改善FLHS。雖然枸杞對於改善FLHS之作用較DHA藻油不顯著,但對DHA藻油改善FLHS之效果亦無負面影響。在蛋品方面,飼糧中添加DHA藻油可增加蛋黃中DHA含量,提升蛋之營養價值,而枸杞可增加蛋黃色澤,提升蛋之經濟價值。因此,DHA藻油及枸杞為安全健康之飼料添加物,可改善產蛋雞FLHS,並生產較高經濟價值之機能蛋。 | zh_TW |
dc.description.abstract | Fatty liver hemorrhagic syndrome (FLHS) is a metabolic diease of laying hens. It is characterized by the accumulation of excess fat in the liver due to enhanced lipogenesis, leading to rupture and hemorrhagic lesions with liver of hens during late laying stage. It tends to result in sudden egg production drop and increase mortality in some serious cases and thus causes considerable economic loss by the egg producers. In this study, we aim to improve FLHS by dietary supplementation of docosahexaenoic acid (DHA) and Goji in laying hens, and furthermore to produce functional eggs in order to increase the value of eggs as well as.
Eighty-four Hy-line strain W-36 layers (36 week of age) were used in the study with a factorial design of dietary supplementation of Algal DHA oil and Goji (Algal DHA oil 0, 1% × Goji 0, 2%). Feed and water were supplied ad libitum during the trial for 24 weeks (36-60 week of age). Results showed that Algal DHA oil and Goji had no effect on the body weight and egg production (P > 0.05). In all treatments, no significant difference in plasma total cholesterol (TC) and triglyceride (TG) level were observed (P > 0.05). Compared to the control diet (Algal DHA oil 0%, Goji 0%), Algal DHA oil treatments decreased hemorrhagic score, TC and TG content and fat accumulation in the liver (P < 0.05) at 60 week of age. Goji treatments reduced hepatic TC (P < 0.05) at 60 week of age. However, there were no significant difference in all treatments along age at 36 and 48 week (P > 0.05). Moreover, Algal DHA oil suppressed hepatic transcript level of lipogenic enzymes, including acyl-CoA oxidase (ACC) and fatty acid synthase (FAS) from 48 to 60 week of age (P < 0.05), consistently with attenuated fat accumulation in the liver. All treatments had no significant effect on the transcript abundance of proinflammatory cytokine genes, such as tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6). In addition, yolk color from hens with dietary supplementation of Goji had higher a (red) and b (yellow) values than those from control treatment. In conclusion, Algal DHA oil and Goji may have the potential to be used as a dietary supplementation to improve FLHS in laying hens, and also increase the economic and nutritional value of the eggs. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T05:07:57Z (GMT). No. of bitstreams: 1 ntu-103-R01626011-1.pdf: 7427054 bytes, checksum: b5c0309d12c411fb82c1f9b0955da7bd (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 目錄
口試委員會審定書 I 謝誌... II 中文摘要 V Abstract VII 圖次... IX 表次... XI 壹、 前言 1 貳、 文獻探討 3 一、 脂肪肝背景介紹 3 (一) 肝臟之功能及重要性 3 (二) 脂肪肝之定義與分類 4 (三) 非酒精性脂肪肝與家禽之關係及應用 6 二、 家禽出血性脂肪肝 7 (一) 出血性脂肪肝之發現及症狀 7 (二) 造成出血性脂肪肝之原因 8 (三) 雞隻脂肪代謝與產蛋 12 三、 二十二碳六烯酸 (DHA) 15 (一) 脂肪酸基本介紹 15 (二) 二十二碳六烯酸 (DHA)之特性與功能 17 (三) n-3多不飽和脂肪酸對家禽脂質代謝之影響 19 四、 枸杞 21 (一) 學名歷史 21 (二) 植物型態 21 (三) 成分 21 (四) 藥理功能 22 參、 材料與方法 25 一、 試驗設計 25 (一) 動物試驗 25 (二) 採樣處理 28 二、 測定項目與分析方法 28 (一) 生長性狀與產蛋性狀 28 (二) 血液分析 28 (三) 肝臟分析 31 (四) mRNA表現分析 34 (五) 蛋品分析 37 (六) 統計分析 40 肆、 結果與討論 41 一、 生長性狀與產蛋性狀 41 (一) 生長性狀 41 (二) 產蛋率 44 (三) 血液分析 47 二、 肝臟分析 50 (一) 出血性脂肪肝-出血評分 50 (二) 出血性脂肪肝-脂肪堆積 54 三、 mRNA表現分析 61 (一) 脂質合成基因 61 (二) 促發炎細胞激素 62 四、 蛋品分析 68 (一) 蛋品質(豪氏單位) 68 (二) 蛋黃色澤 69 (三) 蛋黃DHA 73 伍、 結論 76 陸、 附錄 77 柒、 參考文獻 79 | |
dc.language.iso | zh-TW | |
dc.title | 飼糧中添加DHA與枸杞改善產蛋雞出血性脂肪肝 | zh_TW |
dc.title | Dietary supplementations of DHA and Goji improve fatty liver hemorrhagic syndrome in laying hens | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林美峰(Mei-Feng Lin),陳國隆(Guo-Long Chen),陳洵一(Xun-Yi Chen) | |
dc.subject.keyword | 出血性脂肪肝,產蛋雞,DHA,枸杞,蛋, | zh_TW |
dc.subject.keyword | Fatty liver hemorrhagic syndrome,Laying hens,DHA,Goji,Eggs, | en |
dc.relation.page | 88 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-20 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
顯示於系所單位: | 動物科學技術學系 |
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