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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 繆希椿(Shi-Chuen Miaw) | |
| dc.contributor.author | Yu-Ci Syu | en |
| dc.contributor.author | 徐鈺棋 | zh_TW |
| dc.date.accessioned | 2021-06-16T04:20:19Z | - |
| dc.date.available | 2019-10-09 | |
| dc.date.copyright | 2014-10-09 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-20 | |
| dc.identifier.citation | Apetoh, L., Quintana, F.J., Pot, C., Joller, N., Xiao, S., Kumar, D., Burns, E.J., Sherr, D.H., Weiner, H.L., and Kuchroo, V.K. (2010). The aryl hydrocarbon receptor interacts with c-Maf to promote the differentiation of type 1 regulatory T cells induced by IL-27. Nature immunology 11, 854-861.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55733 | - |
| dc.description.abstract | 巨噬細胞是先天性免疫反應的主要元素之一，他們組成了人體抵抗各式各樣病原體的第一道防線。在先前的研究中，c-Maf曾被報導會直接促進IL-10的表現，及藉由干擾c-Rel進入細胞核來抑制IL-12p35的表現。此外，c-Maf會直接促進F4/80的表現，使巨噬細胞呈現一種已分化完成的表型。當遇到環境中的污染物質-多環芳香烴，巨噬細胞中的c-Maf會與AhR結合共同促進intergrin β7的表現。由於c-Maf在巨噬細胞發育和面對細胞激素、環境汙染物及病原體的反應上扮演重要的角色，我的研究目的便是要深入地了解巨噬細胞中的c-Maf在免疫反應中所扮演的角色。
我們在生物體外極化骨髓幹細胞分化來的巨噬細胞為典型活化巨噬細胞(又稱為M1)和非典型活化巨噬細胞(又稱為M2)。我們發現c-Maf信使核醣核酸的表現量在M0最高，而c-Maf蛋白質的表現量在M1最高。 藉由信使核醣核酸微陣列的分析，我們得到了c-Maf野生型和剔除型的典型活化巨噬細胞和非典型活化巨噬細胞的基因表達譜。由比較c-Maf野生型和剔除型的巨噬細胞，我們可以導出哪些基因是受c-Maf的直接或間接調控。 到目前為止，我們已經檢驗了27個在c-Maf野生型和剔除型巨噬細胞中表現量差異超過兩倍的基因，其中的十個基因確實有顯著差異。其中一個基因為Ccr2，它在c-Maf剔除型的非典型活化巨噬細胞的表現量顯著高於c-Maf野生型的非典型活化巨噬細胞；其他的基因則為IL-6、IL-10、Cx3cr1、IL-12p35、IL-12p40、Clec5a、Tlr12、Runx3和Stat1，它們的表現量在c-Maf剔除型的典型活化巨噬細胞及c-Maf野生型的典型活化巨噬細胞間有顯著的差異。在這些基因中，我們藉由染色質免疫沉澱證實了在IL-12p40和Runx3啟動子上預測的保守c-Maf結合位。這顯示了c-Maf可藉由與IL-12p40和Runx3啟動子上的c-Maf結合位結合來直接地負調控IL-12p40和Runx3的表現。 | zh_TW |
| dc.description.abstract | Macrophages are major elements in innate immunity, and they comprise the first line of defense against various pathogens. In previous studies, c-Maf has been reported to transactivate IL-10 directly and inhibit IL-12p35 expression by interfering c-Rel nuclear translocalization. Moreover, c-Maf transactivates F4/80 and contributes to the terminally differentiated phenotypes of macrophages. In response to polycyclic aromatic hydrocarbon (PAH), an environmental contaminant, c-Maf cooperates with AhR to activate intergrin | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T04:20:19Z (GMT). No. of bitstreams: 1 ntu-103-R00449005-1.pdf: 6838429 bytes, checksum: d2d85e605ad7d512daea0337b5349f5a (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | Abstract………………………………………………………………………………….i
中文摘要 ………………………………………………………………………………iii Contents………………………………………………………………………………...iv List of figures…………………………………………………………………………..vi Chapter 1. Introduction 1 1.1 Overview of the Maf family 1 1.2 Overview of c-Maf transcription factor 3 1.3 c-Maf-targeting genes and its functions 4 1.4 The roles of c-Maf in macrophages 8 1.5 Macrophage subsets 9 1.6 Rationale and experimental designs 10 Chapter 2. Material and methods 12 2.1 Culture of bone marrow-derived macrophages (BM-DMs) 12 2.2 In vitro differentiation of BM-DMs to M1 and M2 subsets 12 2.3 Cell characterization by flow cytometry ……………………………………......13 2.4 Transcriptional profile analysis …………………………………………………13 2.5 Quantitative real-time PCR …………………………………………………......14 2.6 Chromatin immunoprecipitation (ChIP) 15 2.7 Immunoprecipitation and Western blotting …………………………………......17 Chapter 3. Results 19 3.1 c-Maf expression level in macrophages is comparable to T lymphocytes 19 3.2 Among different subsets, mRNA expression level of c-Maf is the highest in M0, whereas protein expression level of c-Maf is the highest in M1……………...19 3.3 The autophagy activity is the highest in M1, which indicates the ability to present antigens is the highest in M1. 21 3.4 Analysis of microarray data of c-Maf+/+ and c-Maf-/- M1 and M2 and confirmation of array results by real-time PCR.. 21 3.4.1 Maf family and other activated protein 1 (AP1) superfamily………………..22 3.4.2 M1 and M2 signature genes………………………………………………….23 3.4.3 Chemokine signaling………………………………………………………...25 3.4.4 Cytokine signaling…………………………………………………………...25 3.4.5 Pattern recognition receptor (PRR) signaling……………………………..…26 3.4.6 Immune defense……………………………………………………………...27 3.4.7 Antigen presentation…………………………………………………………27 3.4.8 Solute carriers or channels…………………………………………………...28 3.4.9 Membrane receptors………………………………………………………….28 3.4.10 Cell adhesion molecules (CAMs)……………………………………………28 3.4.11 Tissue remodeling……………………………………………………………29 3.4.12 Lipid metabolism…………………………………………………………….29 3.4.13 Cell surface receptor-linked signal transduction molecules…………………30 3.4.14 Metabolism…………………………………………………………………..30 3.4.15 Epigenetic and post-translational modification……………………………...31 3.4.16 Coagulation cascades………………………………………………………...31 3.4.17 Regulators of cell cycle………………………………………………………31 3.5 The MARE sites in promoter region of IL-12p40 and Runx3 are confirmed by ChIP assay. 32 Chapter 4. Disscussions……………………………………………………………….34 4.1 Relative expression levels of c-Maf among different macrophage subsets were different between human and mouse………………………………………...34 4.2 There were no differences of F4/80 expression levels between c-Maf+/+ and c-Maf-/- BM-DMs……………………………………………………………35 4.3 There was higher mRNA expression level of IL-12p40 in c-Maf-/- than in c-Maf+/+ M1 macrophages………………………………………………………..........37 Chapter 5. Figures 38 Chapter 6. Table 70 Supplementary Figure 73 References 74 | |
| dc.language.iso | en | |
| dc.subject | 微陣列 | zh_TW |
| dc.subject | c-Maf | zh_TW |
| dc.subject | 巨噬細胞 | zh_TW |
| dc.subject | BMDM | zh_TW |
| dc.subject | 典型活化巨噬細胞 | zh_TW |
| dc.subject | 非典型活化巨噬細胞 | zh_TW |
| dc.subject | c-Maf | zh_TW |
| dc.subject | 巨噬細胞 | zh_TW |
| dc.subject | BMDM | zh_TW |
| dc.subject | 微陣列 | zh_TW |
| dc.subject | 典型活化巨噬細胞 | zh_TW |
| dc.subject | 非典型活化巨噬細胞 | zh_TW |
| dc.subject | M2 | en |
| dc.subject | c-Maf | en |
| dc.subject | M2 | en |
| dc.subject | M1 | en |
| dc.subject | microarray | en |
| dc.subject | BMDM | en |
| dc.subject | macrophage | en |
| dc.subject | c-Maf | en |
| dc.subject | macrophage | en |
| dc.subject | BMDM | en |
| dc.subject | microarray | en |
| dc.subject | M1 | en |
| dc.title | 探討c-Maf調節巨噬細胞中基因的表現 | zh_TW |
| dc.title | c-Maf in regulation of macrophage gene expression | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 伍安怡(Betty A. Wu Hsieh),劉淦光(Gan-Guang Liou),司徒惠康(Huey-Kang Sytwu) | |
| dc.subject.keyword | c-Maf,巨噬細胞,BMDM,微陣列,典型活化巨噬細胞,非典型活化巨噬細胞, | zh_TW |
| dc.subject.keyword | c-Maf,macrophage,BMDM,microarray,M1,M2, | en |
| dc.relation.page | 83 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-20 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 免疫學研究所 | zh_TW |
| 顯示於系所單位： | 免疫學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-103-1.pdf 未授權公開取用 | 6.68 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。