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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
dc.contributor.author | Po-An Yang | en |
dc.contributor.author | 楊博安 | zh_TW |
dc.date.accessioned | 2021-06-16T04:13:48Z | - |
dc.date.available | 2019-09-05 | |
dc.date.copyright | 2014-09-05 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-20 | |
dc.identifier.citation | [1] Minsky, M. 'Memoir on Inventing the Confocal Scanning Microscope.' Scanning 10(4): 128-138. (1988)
[2] Morishige, N. et al. 'Second-Harmonic Imaging Microscopy of Normal Human and Keratoconus Cornea.' Investigative Ophthalmology & Visual Science 48, 1087-1094 (2007). [3] Tan, H. Y. et al. 'Multiphoton Fluorescence and Second Harmonic Generation Imaging of the Structural Alterations in Keratoconus Ex Vivo.' Investigative Ophthalmology & Visual Science 47, 5251-5259 (2006). [4] Teng, S. W. et al. 'Multiphoton Autofluorescence and Second-Harmonic Generation Imaging of the Ex Vivo Porcine Eye.' Investigative Ophthalmology & Visual Science 47, 1216-1224 (2006) [5] Maurice, D. M. 'The structure and transparency of the cornea.' J Physiol 136(2): 263-286 (1957) [6] Roth, S. and I. Freund. 'Optical second-harmonic scattering in rat-tail tendon.' Biopolymers 20(6): 1271-1290 (1981) [7] Smith, J. W. 'The transparency of the corneal stroma.' Vision Res 10(1): 109-110 (1970). [8] W Lo, W. C., CM Hsueh. 'Fast fourier transform–based analysis of second harmonic generation image in keratoconic cornea.' Investigative Ophthalmology & Visual Science 53(7): 3501-3507 (2012). [9] Plotnikov, S. V., et al. 'Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres.' Biophysical Journal 90(2): 693-703 (2006). [10] Borradori, L. and A. Sonnenberg. 'Hemidesmosomes: Roles in adhesion, signaling and human diseases.' Current Opinion in Cell Biology 8(5): 647-656 (1996). [11] Michael T. Heideman, et al. 'Gauss and the History of the Fast Fourier Transform.' Archive for History of Exact Sciences 34(3): 265-277 (1985). [12] J,Fourier. 'Theorie analytique de la chaleur.' (1822) [13] James W. Cooley, J. W. T. 'An Algorithm for the Machine Calculation of Complex Fourier Series.' Mathematics of Computation 19(90): 297-301. (1965) [14] Komai, Y. and T. Ushiki. 'The three-dimensional organization of collagen fibrils in the human cornea and sclera.' Investigative Ophthalmology & Visual Science 32(8): 2244-2258 (1991). [15] Luc M. Vincent, 'Morphological image processing and network analysis of cornea endothelial cell images', Proc. SPIE 1769, Image Algebra and Morphological Image Processing III, 212; doi:10.1117/12.60644 (1992). [16] Linsenmayer, T. F., et al. 'Development and roles of collagenous matrices in the embryonic avian cornea.' Prog Retin Eye Res 17(2): 231-265 (1998). [17] Feuk, T. 'On the transparency of the stroma in the mammalian cornea.' IEEE Trans Biomed Eng 17(3): 186-190 (1970). [18] His W. 'Beitrage zur normalen und pathologischen Histoiogie der Cornea. Berkhauser.' (1856) [19] Stanworth, A. and E. J. Naylor. 'Polarized Light Studies of the Cornea.' Journal of Experimental Biology 30(2): 160-163 (1953). [20] Mega, Y., et al. 'Quantification of lamellar orientation in corneal collagen using second harmonic generation images.' Optics Letters 37(16): 3312-3314 (2012). [21] Hovhannisyan, V. A., et al. 'Spatial orientation mapping of fibers using polarization-sensitive second harmonic generation microscopy.' Journal of Biophotonics 5(10): 768-776 (2012). [22] Robert L. and Alfred J. 'Morphogenesis of the collagenous stroma in the chick cornea' J Cell Bio 50:840-858 (1971) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55631 | - |
dc.description.abstract | 近年來,雙光子光學顯微術時常被使用在生物影像的研究,非線性光學顯微術相較於一般的光學顯微術,它的優點是可以點激發,避開在聚焦點以外的樣本被激發,並且可以使用更高波長的光源激發生物樣本,可以減少對於生物樣本的破壞,以及加深掃描的深度,因此非線性光學顯微術非常適合用在三維生物組織的影像掃描中。
角膜是眼睛的最外層的組織,主要是由膠原蛋白所組成。當角膜內的膠原蛋白結構被破壞時,會嚴重影響人們的視力,甚至失明。想要製造出人造角膜並且用它來治癒因角膜受損而導致的疾病時,必須要有合適的分析方法去研究角膜的結構。在這篇論文中,我們使用二維傅立葉轉換分析角膜中的膠原蛋白排列。藉由二維傅立葉轉換分析雞和吳郭魚眼角膜中膠原蛋白的穿透式二倍頻訊號,我們發現從角膜表層到內層,膠原蛋白呈順時針方向旋轉,而且當在越靠近角膜內層時,旋轉的變化變得越慢。由於角膜中膠原蛋白的反射式二倍頻的訊號較為模糊,所以我們無法藉由二維傅立葉轉換分析得到膠原蛋白的角度,因此我們使用可解析角度之二倍頻分析角膜中的膠原蛋白的角度。可解析角度之二倍頻主要是藉由膠原蛋白的二倍頻強度會隨著入射光的極化角度與膠原蛋白的夾角改變而隨之變化以找出膠原蛋白的方向。實驗後我們發現可以藉由可解析角度之二倍頻分析角膜的反射式二倍頻訊號,找出角膜內的膠原蛋白的排列,這讓我們發現它未來可以被運用在觀察活體的角膜結構生成的可能性。 | zh_TW |
dc.description.abstract | In recent years, two-photon excitation microscopy (TPEM) has been widely used in the biological and medical imaging. Due to the limited sub-femtoliter focal volume, images with high depth-discrimination can be obtained with TPEM. Limited focal volume also significantly reduces off-focal photo-damage. Furthermore, light at longer wavelength allows deeper image penetration into the tissue. These advantages enable non-linear optical microscopy be a popular imaging method in biological sciences.
Cornea, the outermost layer of the eye, is mainly composed of fibrotic collagen. Any distortion of cornea collagen orientation can result in significant visual deterioration. In order to understand how corneal collagen is organized and to fabricate the artificial cornea to cure corneal diseases, it is important to have a technique that is capable of analyzing the structure of the cornea. We first utilizing two dimensional fast fourier transform (2D-FFT) to obtain the structural characteristics of corneal collagen. By using 2D-FFT to analyze forward second harmonic generation (SHG) image of cornea along with depth, we find orientation of collagen fibrils in chicken and Tilapia cornea both rotated counterclockwise when observing from anterior to posterior portion of the cornea. Furthermore, the varying rate of collagen orientation decreases with increasing the depth of cornea. Due to 2D-FFT failing to distinguish homogeneous image such as backward SHG, we apply polarization sensitive second harmonic generation to analyze the cornea structure. By analyzing differences of second harmonic generation (SHG) at different angle between polarization and collagen fibril, the orientation of the collagen fibrils in the cornea can be deduced with pixel-resolution. We find that PSSHG can be used to analyze the backward SHG which suggesting the potential of using PSSHG to observe the corneal development in vivo. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T04:13:48Z (GMT). No. of bitstreams: 1 ntu-103-R01245011-1.pdf: 2458781 bytes, checksum: a3a80634d7bad835353e6ddd234f224f (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 致謝 i
中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vi LIST OF TABLES viii Chapter 1 Introduction 1 Chapter 2 The Basic Principles 3 2.1 Basic Principles of Two-Photon Excitation 3 2.2 The Basic of Second Harmonic Generation 5 2.3 Second Order Susceptibility 7 2.4 Mapping Spatial Orientation of Fibers by Using Polarization-Sensitive SHG Microscope 10 2.5 Fast Fourier Transform (FFT) 11 2.5.1 Discrete Fourier Transform (DFT) 11 2.5.2 Fast Fourier Transform (FFT) 12 Chapter 3 Cornea 14 3.1 The Structure of Cornea 14 3.2 The Optical Properties of Cornea 17 3.2.1 The Transparency of Cornea 17 3.2.2 The Birefringence of Cornea 18 Chapter 4 Methods 19 4.1 Microscope System 19 4.2 Sample Preparation 20 Chapter 5 Collagen Orientation Analysis by Two Dimensional Fast Fourier Transform (2-D FFT) 21 5.1 Determination of Fiber Orientation by 2D-FFT Analysis 21 5.2 Full Depth Orientation Measurement of Chicken, Tilapia and Zebra fish Cornea 25 Chapter 6 Structural Characterization of Collagen Fibrils in Cornea by Polarization Sensitive Second Harmonic Generation (PSSHG) 33 6.1 Structural Characterization of Collagen Fibrils in Tendon 33 6.2 Structural Analysis of The Collagen Fibril in Cornea 38 Chapter 7 Conclusion 40 REFERENCE 41 | |
dc.language.iso | en | |
dc.title | 利用二倍頻顯微術分析角膜中的
膠原蛋白結構與特性 | zh_TW |
dc.title | Structural Observation of Cornea Collagen Organization by Second Harmonic Generation Microscopy | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張顏暉(Yuan-Hue Chang),石明豐(Ming-Feng Shih),陳永芳(Yang-Fang chen) | |
dc.subject.keyword | 二倍頻,膠原蛋白,角膜, | zh_TW |
dc.subject.keyword | Cornea,Second Harmonic Generation,collagen, | en |
dc.relation.page | 43 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-20 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 應用物理所 | zh_TW |
顯示於系所單位: | 應用物理研究所 |
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