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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 盧道杰(Dau-Jye Lu),朱有田(Yu-Ten Ju),袁孝維(Hsiao-Wei Yuan) | |
| dc.contributor.author | Ling Wang | en |
| dc.contributor.author | 王翎 | zh_TW |
| dc.date.accessioned | 2021-06-16T04:10:30Z | - |
| dc.date.available | 2024-08-20 | |
| dc.date.copyright | 2014-08-26 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-21 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55578 | - |
| dc.description.abstract | 石虎（Prionailurus bengalensis）為貓科豹貓屬物種，廣泛分布於亞洲地區，是臺灣僅有的兩種原生貓科動物之一。文獻指出島嶼型的石虎亞種多面臨遺傳變異性低與族群滅絕的風險，然臺灣缺乏相關的遺傳研究。是以本研究分析臺灣石虎粒線體分子標記多樣性與親緣關係，並篩選適用的微衛星分子標記，以檢視臺灣石虎族群的遺傳特性，並建構遺傳分析之基礎。分析68個石虎樣本之粒線體分子標記，發現臺灣石虎族群的粒線體遺傳多樣性偏低（細胞色素b/D-loop單套型歧異度為0.378/0.428；核苷酸歧異度為0.03% /0.12%）。細胞色素b/D-loop單套型有兩個遺傳分群，與地理分布無明顯相關，其中苗栗之遺傳多樣性高於南投。利用中性檢測、核苷酸變異分布與貝氏天際線圖檢視臺灣石虎族群歷史變動，發現距今5,000年前以前的石虎族群大小穩定，其後則有微幅的族群擴張。分析亞種的親緣關係樹（鄰接、最大似然及貝氏）與特徵序列，發現臺灣石虎的所有單套型形成獨立遺傳支系。此外本研究經由跨物種微衛星基因座測試以72%成功率篩選出13組在臺灣石虎族群具有多型性之微衛星基因座，未來若能測試其個體鑑別能力，或可做為有效評估近代演化事件的遺傳分析工具。本研究釐清臺灣石虎族群之遺傳多樣性組成，可做為保育策略擬定與族群遺傳監測之參考。 | zh_TW |
| dc.description.abstract | Leopard cat （Prionailurus bengalensis） is widely distributed in Asia, and it is a rare species protected by the Wildlife Conservation Law in Taiwan. Many studies have stated that island populations are at the risks of low genetic diversity and extinction. To understand the genetic status of leopard cat populations in Taiwan, this study tried to construct the techniques of genetic analysis. Sixty-eight samples were obtained to analyze mitochondrial DNA markers. The low values of haplotype diversity (H=0.378/0.425 in cytb/D-loop) and nucleotide diversity (π=0.03%/0.12% in cytb/D-loop) revealed that population bottleneck effect may have occurred recently. The leopard cat populations in Taiwan lack geography structure; as a result, the populations with relatively high genetic diversity, like Miaoli and Nantou, should be regarded as key groups to protect. The historical demography of leopard cat populations in Taiwan inferred from the neutrality test, mismatch distribution and Bayesian skyline plot indicated a stable population in the past, but slightly expanded since 5,000 years before present. The subspecies phylogenetic tree divided the populations of leopard cats into two clades. And the leopard cat populations in Taiwan form an independent genetic cluster. Results of this study help to clarify the genetic diversity of leopard cat populations in Taiwan, and served as a fundamental data to leopard cat conservation. For a better explanation of evolutionary history of leopard cats, we should collect more samples from each county and China. Additionally, we successfully selected 13 microsatellite loci with polymorphism, which can be regarded as an effective genetic analysis tool. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T04:10:30Z (GMT). No. of bitstreams: 1 ntu-103-R01625003-1.pdf: 1477631 bytes, checksum: 388d80d376a96e846615d8195ad599fa (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
謝誌 III 摘要 V Abstract VI 壹、前言 1 貳、文獻回顧 3 一、石虎之基礎生物學與分類學地位 3 （一）石虎之形態特徵與棲地類型 3 （二）石虎之分類學地位 3 （三）石虎面臨之威脅與保育現況 5 二、臺灣石虎族群現況與保育重要性 6 三、遺傳分析之優勢 8 （一）分子標記的特性與適用性 11 （二）分子標記於石虎遺傳分析之應用 13 參、材料與方法 17 一、遺傳樣本收集與保存 17 （一）樣本收集 17 （二）DNA萃取 17 二、粒線體分子標記之建立與分析 19 （一）粒線體分子標記之引子設計 19 （二）以聚合酶鏈鎖反應增幅石虎之粒線體DNA序列 20 （三）粒線體DNA序列增幅產物之純化與定序 22 （四）粒線體基因組全長、細胞色素b及D-loop序列之收集與整理 22 （五）粒線體基因組全長、細胞色素b及D-loop序列排列比對 23 （六）細胞色素b及D-loop序列之族群遺傳分析 24 三、微衛星分子標記之建立與分析 27 （一）微衛星分子標記之引子設計 28 （二）跨物種微衛星分子標記之選殖 28 （三）微衛星分子標記之聚合酶鏈鎖反應與毛細管電泳 29 （四）微衛星分子標記之波形判讀與多型性分析 29 肆、結果 31 一、臺灣石虎之粒線體基因組全長定序與結構比較 31 （一）臺灣石虎與近緣物種之鹼基對組成比較 31 （二）臺灣石虎與阿穆爾豹貓之基因配置比較 31 二、粒線體分子標記之臺灣石虎族群遺傳分析 32 （一）粒線體細胞色素b與D-loop之遺傳多樣性分析 32 （二）臺灣石虎族群之族群歷史變動 33 （三）臺灣石虎族群與其他石虎亞種之親緣關係 34 三、跨物種微衛星基因座的篩選及微衛星多型性與遺傳多樣性分析 36 （一）跨物種微衛星基因座選殖與波形判讀 36 （二）多型性與遺傳多樣性分析 37 伍、討論 39 一、臺灣石虎粒線體基因組全長與近緣物種有相似的序列組成 39 二、臺灣石虎族群遺傳多樣性偏低 39 三、臺灣石虎族群具穩定族群歷史變動 40 四、臺灣石虎族群具有獨立的遺傳類群可作為法醫學鑑定依據 41 五、臺灣石虎族群與石虎亞種間之族群演化歷史 42 六、跨物種微衛星基因座測試與後續可應用性 43 七、研究限制 45 陸、結論 47 柒、參考文獻 49 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用粒線體與微衛星分子標記分析臺灣石虎族群遺傳結構 | zh_TW |
| dc.title | Using Mitochondrial DNA and Microsatellite Markers Analyze the Population Structure of Leopard Cat (Prionailurus bengalensis chinensis) in Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 裴家騏(Kurtis Jai-Chyi Pei),林思民(Si-Min Lin) | |
| dc.subject.keyword | 遺傳多樣性,粒線體分子標記,微衛星分子標記,石虎保育, | zh_TW |
| dc.subject.keyword | genetic diversity,mitochondrial DNA markers,microsatellite markers,leopard cat conservation, | en |
| dc.relation.page | 100 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-21 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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