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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55281完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張惠婷(Hui-Ting Chang) | |
| dc.contributor.author | Wei-Che Chien | en |
| dc.contributor.author | 簡偉哲 | zh_TW |
| dc.date.accessioned | 2021-06-16T03:54:38Z | - |
| dc.date.available | 2024-12-27 | |
| dc.date.copyright | 2015-02-04 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2014-12-28 | |
| dc.identifier.citation | 尹華文（2002）應用超臨界流體技術萃取植物精油。林業研究專訊。15（3）:43-45。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55281 | - |
| dc.description.abstract | 本研究利用固相微萃取法、水蒸餾法與超臨界二氧化碳流體萃取法萃取台灣冷杉（Abies kawakamii (Hay.) Ito）葉子，並以氣相層析-質譜進行化學成分分析，此外，亦評估台灣冷杉精油及超臨界萃取物的生物活性。研究結果顯示台灣冷杉葉子的揮發性成分以單萜類化合物為主，主要成分為α-Pinene、Camphene、Limonene、β-Phellandrene與Bornyl acetate。台灣冷杉葉子以水蒸餾法萃取之精油收率為1.45%，精油中含量較多的成分為α-Pinene、Camphene與Bornyl acetate；台灣冷杉葉子以超臨界二氧化碳流體萃取法萃取最佳收率為1.56%（萃取壓力為250 Bar，萃取溫度為50℃），主成分為α-Eudesmol、Bornyl acetate、Maltol及Caryophyllene oxide。在生物活性評估方面，台灣冷杉葉子精油和超臨界二氧化碳流體萃取物具有紙張防黴、海蝦致死活性及延長秀麗隱桿線蟲（Caenorhabditis elegans）生命週期等生物活性。 | zh_TW |
| dc.description.abstract | The purposes of this study are to extract the volatile organic compounds from Abies kawakamii (Hay.) Ito leaf by solid phase microextraction sampling, hydrodistillation and supercritical carbon dioxide fluid extraction, to identify the constituents of these extracts by GC-MS analysis; and to evaluate the bioactive activities of essential oil and supercritical carbon dioxide fluid extracts. Results showed that monoterpenoids were the main constituents of volatile organic compounds (VOCs) from A. kawakamii leaf, including α-pinene, camphene, limonene, β-phellandrene, and bornyl acetate. The yield of essential oil from Abies kawakamii leaf was 1.45%, and the main constituents of essential oil were α-pinene, camphene, and bornyl acetate. The highest yield of supercritical carbon dioxide fluid extract (250 Bar, 50℃) from A. kawakamii leaf was 1.56%; and its main constituents were α-eudesmol, bornyl acetate, maltol, and caryophyllene oxide. According to the results of bioactivity assay, the essential oil and supercritical carbon dioxide extract from A. kawakamii leaf exhibited the antimildew activity, shrimp lethality activity, and the positive effect on lifespan extension in Caenorhabditis elegans. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T03:54:38Z (GMT). No. of bitstreams: 1 ntu-104-R96625040-1.pdf: 4848344 bytes, checksum: 2f37bf3e900301efec36686116a57aaa (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 目錄.....I
圖目錄.....IV 表目錄.....VI 摘要.....IX Abstract.....X 壹、前言.....1 貳、文獻回顧.....2 一、冷杉屬植物.....2 （一）、冷杉屬植物及台灣冷杉簡介.....2 （二）、冷杉屬植物成分與生物活性.....3 1.冷杉屬植物成分.....3 2.冷杉屬植物活性.....7 二、超臨界萃取簡介及原理.....15 （一）、植物萃取.....15 （二）、超臨界二氧化碳流體萃取.....21 1.超臨界二氧化碳流體原理.....21 2.超臨界二氧化碳的應用.....26 參、材料與方法.....28 一、試驗材料.....28 （一）、試材.....28 （二）、試驗藥品與溶劑.....28 1.藥品......28 2.溶劑....28 3.紙樣....28 4.試驗菌種.....28 二、試驗方法.....29 （一）、揮發性成分萃取與分析.....29 （二）、精油萃取.....29 （三）、超臨界二氧化碳流體萃取.....29 （四）、氣相層析-質譜分析....29 （五）、抗氧化活性試驗......30 1.還原力評估.....30 2.DPPH自由基清除試驗.....30 （六）、海蝦致死試驗.....31 （七）、抗腐朽菌活性試驗.....31 （八）、紙張防黴菌試驗.....31 （九）、線蟲延長壽命試驗.....31 （十）、統計分析.....32 肆、結果與討論.....33 一、台灣冷杉葉子揮發性物質之成分分析.....33 （一）、頂空氣相層析質譜分析.....33 （二）、頂空固相微萃取之氣相層析質譜分析.....37 二、台灣冷杉葉子精油之成分分析.....42 （一）、台灣冷杉葉子精油之成分分析.....42 （二）、台灣冷杉葉子揮發性物質與精油之化學組成比較.....44 三、台灣冷杉葉子超臨界二氧化碳流體萃取之成分與效率分析 .....45 （一）、台灣冷杉葉子超臨界二氧化碳流體萃取法萃取效率.....45 （二）、台灣冷杉葉子超臨界萃取物.....47 1.台灣冷杉葉子超臨界萃取物之成分分析.....47 2.台灣冷杉超臨界萃取物與精油之化學成分比較.....49 四、台灣冷杉葉子精油與超臨界萃取物之生物活性探討.....50 （一）、抗氧化活性評估.....50 1.還原力能力.....50 2.DPPH自由基清除活性.....51 （二）、海蝦致死活性評估.....52 （三）、抗腐朽菌活性評估.....53 （四）、紙張防黴菌活性評估.....54 （五）、線蟲延長壽命活性評估.....55 伍、結論.....58 陸、參考文獻.....59 | |
| dc.language.iso | zh-TW | |
| dc.subject | 秀麗隱桿線蟲 | zh_TW |
| dc.subject | 台灣冷杉 | zh_TW |
| dc.subject | 超臨界二氧化碳流體萃取法。 | zh_TW |
| dc.subject | 固相微萃取 | zh_TW |
| dc.subject | 水蒸餾法 | zh_TW |
| dc.subject | 精油 | zh_TW |
| dc.subject | Supercritical carbon dioxide fluid extraction. | en |
| dc.subject | Caenorhabditis elegans | en |
| dc.subject | Essential oil | en |
| dc.subject | Hydrodistillation | en |
| dc.subject | Solid phase microextraction | en |
| dc.subject | Abies kawakamii | en |
| dc.title | 台灣冷杉葉子精油與超臨界萃取物之成分與生物活性研究 | zh_TW |
| dc.title | Studies on the Constituents and Bioactivities of
Essential Oil and Supercritical Fluid Extract from Abies kawakamii Leaf | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張上鎮(Shang-Tzen Chang),葉汀峰(Ting-Feng Yeh) | |
| dc.subject.keyword | 台灣冷杉,秀麗隱桿線蟲,精油,水蒸餾法,固相微萃取,超臨界二氧化碳流體萃取法。, | zh_TW |
| dc.subject.keyword | Abies kawakamii,Caenorhabditis elegans,Essential oil,Hydrodistillation,Solid phase microextraction,Supercritical carbon dioxide fluid extraction., | en |
| dc.relation.page | 65 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-12-29 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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