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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55167完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 廖秀娟(Vivian Hsiu-Chuan Liao) | |
| dc.contributor.author | Chi-Wei Huang | en |
| dc.contributor.author | 黃紀惟 | zh_TW |
| dc.date.accessioned | 2021-06-16T03:49:43Z | - |
| dc.date.available | 2018-03-13 | |
| dc.date.copyright | 2015-03-13 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-01-23 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55167 | - |
| dc.description.abstract | 砷為重金屬污染物之一，為已知人體致癌物。許多研究指出，使用含砷地下水做為飲用水、灌溉水已在世界各地造成人類健康危害。現行砷污染檢測技術雖具有相當高的靈敏度與準確度，但應用於大規模即時檢測及其價格，仍有其限制性。因此，本研究開發快速及經濟現地檢測地下水中砷之細菌生物感測器。本研究使用非致病菌E. coli DH5α為宿主(host cell)，利用arsR與lacZ之基因組合建構，以顏色改變為其訊號基礎，發展利用目測或光度計檢測水樣中砷之生物感測器。本研究建構之生物感測器，其定量範圍為10至500 μg/l之間，檢測時間約為1到3小時。本研究亦分析細菌數量(以OD600值表示)對於生物感測器呈色能力之差異，結果顯示，較高OD600值之生物感測器，呈色強度亦較高，且檢測時間較短，但量測值變動較大。本研究所建構之生物感測器應用於含砷地下水樣品分析，成功檢測三價砷濃度。另外，本研究分析不同保存條件，以及不同OD600值，在保存期限上的差異。結果顯示，不同OD600值對於保存期限影響不明顯；其中，以液體保存於4℃，具有最長之保存期限，約為九天，其餘保存方式之保存期限，約為三至四天左右。本研究建構之生物感測器，具有低成本、可定量，以及操作簡單等優勢，能應用於砷污染調查檢測，結合現有化學檢測技術，可提升環境污染管理之效率。 | zh_TW |
| dc.description.abstract | Arsenic is one of the heavy metal pollutants and a known human carcinogen. Using arsenic contaminated groundwater for drinking or irrigation has caused major health problems for humans around the world. Current arsenic detecting techniques based on physicochemical methods have high sensitivity and accuracy. Yet, in addition to high cost, the applications to large scale detection were still limited. Therefore, this study developed a color-based bacterial biosensor which is easy and inexpensive for arsenic screening and could be complementary to physicochemical methods. The arsR-lacZ recombinant gene cassette in nonpathogenic strain E. coli DH5α was used in the color-based biosensor which could be measured by eyes or spectrometer. The developed bacterial biosensor demonstrates a quantitative ranging from 10 - 500 μg/l in 1 - 3 hours reaction time. In addition, the result showed that biosensor with higher OD600 has higher induction and lower response time, but lower accuracy. Furthermore, the biosensor was able to successfully detect and estimate arsenic concentration in groundwater samples. The result further showed that OD600 did not affect the shelf life of biosensor. Among different storage methods, biosensor in liquid at 4℃ showed the longest shelf life about 9 days, whereas others were about 3 - 4 days. In conclusion, this study showed that the arsenic biosensor with reliable color signal makes it useful for large scale rapid screening of arsenic pollutants providing the potential for better management strategies for environmental quality control. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T03:49:43Z (GMT). No. of bitstreams: 1 ntu-104-R01622048-1.pdf: 4942006 bytes, checksum: c5d08580711b6b0e71f75c948b0f96bd (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 致謝 I
中文摘要 II ABSTRACT III 目錄 IV 圖次 VII 表次 VIII 縮寫表 IX 第一章 研究動機 1 第二章 文獻回顧 2 2.1 砷 2 2.1.1 砷在環境中的流布 2 2.1.2 砷的危害性 3 2.1.3 細菌體內砷的調控機制 4 2.1.4 傳統化學法檢測砷污染 5 2.2 生物感測器(biosensor) 6 2.2.1 無細胞型生物感測器(cell-free-based biosensor) 7 2.2.2 廣效性生物感測器(non-specific biosensor) 7 2.2.3 專一性生物感測器(specific biosensor) 8 2.2.4 報告基因(reporter gene) 10 2.2.5 生物感測器的保存(preservation) 12 第三章 研究目的 13 第四章 材料與方法 14 4.1 實驗流程圖 14 4.2 實驗藥品 15 4.3 生物感測器設計與建構 15 4.3.1 菌種與質體 15 4.3.2 勝任細胞製備 15 4.3.3 重組質體建構與轉型 16 4.4 生物感測器劑量與反應關係測試 16 4.5 不同OD600值造成生物感測器之反應差異分析 17 4.6 生物感測器應用於地下水樣品測試 17 4.6.1 採樣與化學分析 17 4.6.2 生物感測器分析 17 4.7 保存條件與時間(shelf life)分析與比較 19 4.7.1 液體保存 19 4.7.2 Cell pellet保存 19 4.8 統計分析 19 第五章 結果 20 5.1 砷生物感測器質體架構 20 5.2 生物感測器與砷劑量及反應關係 20 5.3 不同OD600值對生物感測器呈色能力之影響 25 5.4 生物感測器應用於含砷地下水樣品檢測 27 5.5 不同保存條件對生物感測器保存期限之影響 31 第六章 討論 36 6-1 其他砷污染檢測技術與本研究成果之比較 36 6-1-1 本研究成果概述 36 6-1-2 與傳統化學法之比較 36 6-1-3 與其他研究之生物感測器之比較 38 6-2 本研究成果之應用性 40 6-2-1 OD600值對生物感測器呈色能力之影響 40 6-2-2 保存條件對於生物感測器之應用性分析 40 6-2-3 生物感測器與化學分析法之成本分析比較 41 第七章 結論 43 第八章 建議 44 第九章 參考文獻 45 第十章 附錄 59 | |
| dc.language.iso | zh-TW | |
| dc.title | 研發可利用顏色差異檢測砷之細菌生物感測器 | zh_TW |
| dc.title | Development of Color-Based Bacterial Biosensor for Arsenic Detection | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳昭瑩(Chao-Ying Chen),沈偉強(Wei-Chiang Shen),童心欣(Hsin-Hsin Tung) | |
| dc.subject.keyword | 生物感測器,砷污染,顏色差異訊號,保存期限,地下水, | zh_TW |
| dc.subject.keyword | biosensor,arsenic pollutants,color-based detection,shelf life,groundwater, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-01-23 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物環境系統工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物環境系統工程學系 | |
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