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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳俊宏 | |
| dc.contributor.author | Yu-Chih Lo | en |
| dc.contributor.author | 羅友志 | zh_TW |
| dc.date.accessioned | 2021-06-16T03:47:33Z | - |
| dc.date.available | 2020-03-13 | |
| dc.date.copyright | 2015-03-13 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-01-28 | |
| dc.identifier.citation | 王漢昇， 2009。飼料中添加不同來源及比例之脂質在不同溫度下對紅色吳郭魚成長及魚體脂肪酸組成之影響。國立台灣大學漁業科學研究所碩士論文。
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Eur J Biochem 252: 608–611. Pough FH, Andrews RM, Cadle JE, Crump ML, Savitzky AH & Wells KD. 1998. Herpetology. Prentice-Hall, Inc. New Jersey. pp. 158-164. 196-203. MR Shahmansouri, H Pourmoghadas,AR Parvaresh & H Alidadi, 'Heavymetals bioaccumulation by Iranian and Australian earthworms (Eisenia fetida) in the sewage sludge vermicomposting',Iranian J Env Health Sci Eng, Vol. 2, No.1, pp. 28-32, 2005. Shen HP, Tsai CF & Tsai SC. 2002. Description of a new earthworm belonging to the genus Amynthas (Oligochaeta: Megascolecidae) from Taiwan and its infraspecific variation in relation to elevation. Raffles Bull. Zool. 50(1): 1-8. Sinclair BJ & Renault D. 2010. Intracellular ice formation in insects: Unresolved after 50 years? Comp Biochem Physiol A Mol Integr Physio 155(1): 14-18. Timothy JM, Jon PC & RE Lee. 2009. Brief Chilling to Subzero Temperature Increases Cold Hardiness in the Hatchling Painted Turtle (Chrysemys picta). Physiol Biochem Zool 83(1): 174–181. Takeuchi T. 1988. 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Preparation of methyl esters of fatty acids for gas liquid chromatography, Anal. Chem., 33: 1512-1514. Wang D & Z Wang. 1996. Seasonal Variations in thermogenesis and energy requirements of plateau pikas Ochotona curzoniae and root voles Microtus oeconomus. Acta Theriol 41(3): 225-236. Wagner EL & Gleeson TT. 1997. Postexercise thermoregulatory behavior and recovery from exercise in desert iguanas. Physiology and Behavior. 61: 175-180. Wynne-Edwards KE. 1998. Evolution of parental care in Phodopus : Conflict between adaptations for survival and adaptations for rapid reproduction. Amer. Zool., 38 : 238-250. Watts PD, Oritsland NA, Jonkel C & Ronald K (1981). 'Mammalian hibernation and the oxygen consumption of a denning black bear (Ursus americanus)'. Comp Biochem Physiol A Physiol 69 (1): 121–3. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55109 | - |
| dc.description.abstract | 本研究的主要目的在於探討蚯蚓體內的脂肪酸組成是否會改變以因應環境溫度的變化。由於已知細胞膜不飽和脂肪酸特別是EPA (Eicosapentaenoic acid)及DHA (Docosahexaenoic acid)比例越高，細胞越不容易被冰凍所傷害個體的抗寒能力也越強，因此本研究擬藉由測量蚯蚓的脂肪酸組成來探討其抗寒能力，並且評估外來種黃頸蜷蚓(Pontoscolex corethrurus)未來是否可能入侵到較高海拔的環境。實驗方法是在不同季節的高海拔及中海拔採集棋盤遠環蚓(Amynthas tessellatus tessellatus)，帶回實驗室分析其脂肪酸的組成。另將平地黃頸蜷蚓帶回實驗室以不同溫度飼養後分析其脂肪酸的組成。結果顯示合歡山棋盤遠環蚓在低溫時不飽和脂肪酸比例較高，相對的飽和脂肪酸較低且高海拔蚯蚓的DHA較中低海拔蚯蚓高，冬季時DHA較夏季時高，但黃頸蜷蚓蜷無此現象。再者黃頸蜷蚓的總脂肪酸組成比例並不會因飼養於低溫而有顯著差異，因此黃頸蜷蚓可能無法在短期內調節脂肪酸組成來適應低溫環境，由此推測黃頸蜷蚓在台灣要擴散到高海拔地區是有困難的。 | zh_TW |
| dc.description.abstract | The aim of my research is to understand the relationship between the composition changes of cellular lipids in earthworms and their cold resistance. It has been well known that the lipid bilayer membrane with more unsaturated fatty acid including EPA (Eicosapentaenoic acid) & DHA (Docosahexaenoic acid) can resist lower temperature damage. Therefore the fatty acid composition of cell membrane is a suitable index to evaluate the ability of cold resistancen in animals. To find out whether does the earthworm’s cellular lipids compositions change in low temperature, earthworms were collocated from different seasons and different altitudes, or were kept and acclimated at different temperatures. The results showed that Amynthas tessellatus tessellatus has higher proportion of unsaturated fatty acids and lower the proportion of saturated fatty acids at low temperature. Especialy, the amount of DHA in A. tessellatus tessellatus from high altitude was higher than that from media altitude. Also, the amount of DHA in those earthworms collected from winter time was higher than that from summer time. The total fatty acid composition in Pontoscolex corethrurus had no significant difference under different temperature acclimatuon. Because Pontoscolex corethrurus does not have physiological mechanism to regulate their fatty acid composition during temperature changing situation. It might infer that Pontoscolex corethrurus should be difficult to spread to higher altitude areas in Taiwan. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T03:47:33Z (GMT). No. of bitstreams: 1 ntu-104-R97b41033-1.pdf: 4434639 bytes, checksum: 227043ce1b74c88df7822262da32fee5 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 目錄 頁次
中文摘要 ……………………………………………………………I 英文摘要 …………………………………………………………II 圖目錄 ……………………………………………………………VII 壹、 前言 ………………………………………………………… 1 一、 動物對低溫的適應 ………………………………… 2 二、 動物細胞對低溫的調整 ………………………………… 4 三、 蚯蚓對低溫的適應 …………………………………… 6 四、 台灣魚類對低溫的適應 ……………………………… 6 五、 台灣蚯蚓對低溫的適應 ………………………………… 7 貳、 研究目的 …………………………………………………… 9 參、 材料與方法 ………………………………………………… 10 一、 實驗動物 ……………………………………………… 10 I. 棋盤遠環蚓 ……………………………………… 10 II. 黃頸蜷蚓 ………………………………………… 11 二、 樣區介紹 ……………………………………………… 12 I. 合歡山 …………………………………………… 12 II. 清境農場 …………………………………………… 12 III. 國立台灣大學 ……………………………………… 13 三、 棋盤遠環蚓的採集與生態調查 ……………………… 13 I. 高海拔採樣 (近3000公尺)合歡山區 ………… 13 II. 中海拔採樣 (近2000公尺)清境農場區 ……… 14 四、 黃頸蜷蚓的採集、飼養與處裡 ……………………… 15 I. 黃頸蜷蚓採集 …………………………………… 15 II. 黃頸蜷蚓飼養方法與處理 ……………………… 15 五、 脂肪酸萃取 …………………………………………… 16 I. 粗脂肪萃取 ……………………………………… 16 II. 皂化 ……………………………………………… 16 III. 甲基酯化 ………………………………………… 17 六、 脂肪酸分析 …………………………………………… 17 七、 統計分析 ……………………………………………… 18 肆、 實驗結果 ………………………………………………… 19 一、 季節變化的環境因子對棋盤遠環蚓族群的影響 … 19 二、 季節變化對棋盤遠環蚓脂肪酸組成的影響 ……… 20 三、 海拔變化對棋盤遠環蚓脂肪酸組成的影響 ……… 21 四、 不同溫度飼養黃頸蜷蚓的相對存活率 …………… 22 五、 不同溫度飼養黃頸蜷蚓對脂肪酸組成的影響 …… 23 六、 不同溫度飼養黃頸蜷蚓對個別脂肪酸組成的影響23 伍、 討論 ……………………………………………………… 25 一、 季節變化的環境因子對棋盤遠環蚓族群的影響 … 25 二、 棋盤遠環蚓脂肪酸變化與抗寒生理之關聯 ……… 26 三、 黃頸蜷蚓脂肪酸變化與抗寒生理之關聯 ………… 29 陸、 結論 ……………………………………………………… 31 柒、 參考文獻 …………………………………………………… 32 圖目錄 頁次 圖1、實驗動物 …………………………………………………… 38 圖2、採集地所在位置 …………………………………………… 39 圖3、合歡山地區2005四月到2009四月土壤下10公分平均土溫及 平均雨量 ………………………………………………………40 圖4、合歡山高海拔樣區畫分 ……………………………………… 41 圖5、不同季節合歡山箭竹林與芒草原棋盤遠環蚓密度 ………… 42 圖6、不同季節合歡山箭竹林與芒草原環境因子 ………………… 43 圖7、高海拔棋盤遠環蚓在不同季節飽和脂肪酸與不飽和脂肪酸百分 比 …………………………………………………………… 45 圖8、中海拔棋盤遠環蚓在不同季節飽和脂肪酸與不飽和脂肪酸百分比 …………………………………………………………… 46 圖9、低海拔黃頸蜷蚓在不同季節飽和脂肪酸與不飽和脂肪酸百分比 …………………………………………………………… 47 圖10、高海拔棋盤遠環蚓在不同季節之各個脂肪酸比例 …… 48 圖11、中海拔棋盤遠環蚓在不同季節之各個脂肪酸比例 …… 49 圖12、低海拔黃頸蜷蚓在不同季節之各個脂肪酸比例 …… 50 圖13、不同溫度飼養下黃頸蜷蚓與前二週的相對存活率 …… 51 圖14、不同溫度飼養下黃頸蜷蚓飽和脂肪酸與不飽和脂肪酸百分比 …………………………………………………………… 52 圖15、定溫(17℃)飼養對照組黃頸蜷蚓個別脂肪酸百分比 …… 54 圖16、溫度遞減低溫飼養組黃頸蜷蚓個別脂肪酸百分比 ……… 55 圖17、溫度遞增高溫飼養組黃頸蜷蚓個別脂肪酸百分比 ……… 56 | |
| dc.language.iso | zh-TW | |
| dc.title | 蚯蚓的脂肪酸組成在不同季節與海拔對於抗寒的影響 | zh_TW |
| dc.title | Lipid composition for freeze-tolerance at different seasons and altitudes in the earthworms | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 廖文亮,陳瑞芬 | |
| dc.subject.keyword | 蚯蚓,飽和脂肪酸,不飽和脂肪酸,脂肪酸組成,抗寒生理, | zh_TW |
| dc.subject.keyword | earthworm,saturated fatty acids,unsaturated fatty acids,lipid composition,freeze-tolerance, | en |
| dc.relation.page | 56 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-01-29 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| 顯示於系所單位： | 生命科學系 | |
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