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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 田維誠(Wei-Cheng Tian) | |
dc.contributor.author | Po-Kai Huang | en |
dc.contributor.author | 黃柏愷 | zh_TW |
dc.date.accessioned | 2021-06-16T03:39:06Z | - |
dc.date.available | 2020-01-31 | |
dc.date.copyright | 2015-03-02 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-02-24 | |
dc.identifier.citation | [1] A. Jemal, R. Siegel, E. Ward, Y. Hao, J. Xu, and M.J. Thun, “Cancer Statistics, 2009”, CA Cancer J. Clin., vol. 59, pp. 225-249, 2009.
[2] International Agency for Research on Cancer, “Latest world cancer statistics in 2012”, 2014. [3] 行政院衛生署,“中華民國102年死因統計”, 2014. [4] A. Amann, M. Corradi, P. Mazzone, and A. Mutti, “Lung cancer biomarkers in exhaled breath”, Expert Rev. Mol. Diagn., vol. 11, pp. 207-217, 2011. [5] P.J. Mazzone, “Exhaled breath volatile organic compound biomarkers in lung cancer”, J. Breath Res., vol. 6, 027106, 2012. [6] J.K. Schubert, W. Miekisch, K. Geiger, and G.F.E. Noldge-Schomburg, “Breath analysis in critically ill patients: potential and limitations”, Expert Rev. Mol. Diag., vol. 4, pp. 619-629, 2004. [7] C.S.J. Probert, I. Ahmed, T. Khalid, E. Johnson, S. Smith, and N. Ratcliffe, “Volatile Organic Compounds as Diagnostic Biomarkers in Gastrointestinal and Liver Diseases”, J. Gastrointest. Liver Dis., vol. 18, pp. 337-343, 2009. [8] M. Phillips, J. Herrera, S. Krishnan, M. Zain, J. Greenberg, and R.N. Cataneo, “Variation in volatile organic compounds in the breath of normal humans”, J. Chromatography B, vol. 729, pp. 75-88, 1999. [9] K.-H. Kim, S.A. Jahan, and E. Kabir, “A review of breath analysis for diagnosis of human health”, TrAC Trends in Analytical Chemistry, vol. 33, pp. 1-8, 2012. [10] 蔡蘊明, '氣相層析儀簡介,' Available: http://www.ch.ntu.edu.tw/~chemedu3/Lecture/GC.htm. [11] S. B. Bertman, M. P. Buhr, and J. M. Roberts, 'Automated cryogenic trapping technique for capillary GC analysis of atmospheric trace compounds requiring no expendable cryogens: Application to the measurement of organic nitrates,' Anal. Chem., vol. 65, pp. 2944-2946, 1993. [12] D. Helmig, and J. P. Greenberg, 'Automated in situ gas chromatographic-mass spectrometric analysis of ppt level volatile organic trace gases using multistage solid-adsorbent trapping,' Journal of Chromatography A, vol. 677, pp. 123-132, 1994. [13] Gorecki, T., and Pawliszyn, J., “Sample Introduction Approaches for Solid Phase Microextraction/Rapid GC,” Anal. Chem., vol. 67, pp. 3265-3274, 1995. [14] 林彥宏,VOC前濃縮與預警系統之建構,國立中央大學化學研究所碩士論文,2000 [15] 簡日昇,微型氣相層析儀,國立台灣師範大學化學研究所博士論文,2013 [16] Harvey, D., 'GC Columns', Available: http://community.asdlib.org/imageandvideoexchangeforum/2011/06/21/gc-columns/. [17] S. C. Terry, J. H. Jerman, and J. B. Angell, 'A gas chromatographic air analyzer fabricated on a silicon wafer,' Electron Devices, IEEE Transactions on, vol. 26, pp. 1880-1886, 1979. [18] 張正義,以奈米金單層膜保護團簇塗佈於堆疊式電極結構之揮發性有機化合物氣體感測器,國立台灣大學電機資訊學院電子工程學研究所碩士論文,2013 [19] C.-J. Lu, W. H. Steinecker, W.-C. Tian, M. C. Oborny, J. M. Nichols, M. Agah, et al., 'First-generation hybrid MEMS gas chromatograph,' Lab on a Chip, vol. 5, pp. 1123-1131, 2005. [20] M. Agah, G. R. Lambertus, R. Sacks, and K. Wise, 'High-Speed MEMS-Based Gas Chromatography,' Microelectromechanical Systems, vol. 15, pp. 1371 - 1378, Oct. 30 2006. [21] J. Ji, C. Deng, W. Shen, and X. Zhang, 'Field analysis of benzene, toluene, ethylbenzene and xylene in water by portable gas chromatography–microflame ionization detector combined with headspace solid-phase microextraction,' Talanta, vol. 69, pp. 894-899, 6/15/ 2006. [22] E. T. Zellers, S. Reidy, R. A. Veeneman, R. Gordenker, W. H. Steinecker, G. R. Lambertus, et al., 'An integrated micro-analytical system for complex vapor mixtures,' in Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International, pp. 1491-1496, 2007. [23] R. J. Gordenker, and K. D. Wise, 'A programmable palm-size gas analyzer for use in micro-autonomous systems,' in SPIE Defense, Security, and Sensing, 2012, pp. 83731O-83731O-6, 2012. [24] W. R. Collin, G. Serrano, L. K. Wright, H. Chang, N. Nunovero, and E. T. Zellers, 'Microfabricated gas chromatograph for rapid, trace-level determinations of gas-phase explosive marker compounds,' Anal. Chem., vol. 86, pp. 655-663, 2013. [25] R.-S. Jian, Y.-S. Huang, S.-L. Lai, L.-Y. Sung, and C.-J. Lu, 'Compact instrumentation of a μ-GC for real time analysis of sub-ppb VOC mixtures,' Microchemical Journal, vol. 108, pp. 161-167, 2013. [26] C. Jung Lu, and E. T. Zellers, 'Multi-adsorbent preconcentration/focusing module for portable-GC/microsensor-array analysis of complex vapor mixtures,' Analyst, vol. 127, pp. 1061-1068, 2002. [27] C.-J. Lu, and E. T. Zellers, 'A Dual-Adsorbent Preconcentrator for a Portable Indoor-VOC Microsensor System,' Analytical Chemistry, vol. 73, pp. 3449-3457, 2001. [28] National Instrument, 'Field Wiring and Noise Considerations for Analog Signals', Available: http://www.ni.com/white-paper/3344/en/ [29] C.A. Neugebauer, and M.B. Webb, “Electrical Conduction Mechanism in Ultrathin, Evaporated Metal Films”, J. App. Phys., vol. 33, pp. 74-82, 1962. [30] W.P. Wuelfing, S.J. Green, J.J. Pietron, D.E. Cliffel, and R.W. Murray, “Electronic Conductivity of Solid-State, Mixed-Valent, Monolayer-Protected Au Clusters”, J. Am. Chem. Soc., vol. 122, pp. 11465-11472, 2000. [31] W.P. Wuelfing, and R.W. Murray, “Electron Hopping through Films of Arenthiolate Monolayer-Protected Gold Clusters”, J. Phys. Chem. B, vol. 106, pp. 3139-3145, 2002. [32] EQUIPCO, ' Introduction to Photoionization', Available: http://www.equipcoservices.com/support/tutorials/introduction-to-photoionization/ [33] W.H. Steinecker, M.P. Rowe, and E.T. Zellers, “Model of Vapor-Induced Resistivity Changes in Gold−Thiolate Monolayer-Protected Nanoparticle Sensor Films”, Anal. Chem., vol. 79, pp. 4977-4986, 2009. [34] Q.-Y. Cai, and E.T. Zellers, “Dual-Chemiresistor GC Detector Employing Monolayer-Protected Metal Nanocluster Interfaces”, Anal. Chem., vol. 74, pp. 3533-3539, 2002. [35] R.-S. Jian, R.-X. Huang, and C.-J. Lu, “A micro GC detector array based on chemiresistors employing various surface functionalized monolayer-protected gold nanoparticles”, Talanta, vol. 88, pp. 160-167, 2012. [36] F.-Y. Chen, W.-C. Chang, R.-S. Jian, and C.-J. Lu, “Novel Gas Chromatographic Detector Utilizing the Localized Surface Plasmon Resonance of a Gold Nanoparticle Monolayer inside a Glass Capillary”, Anal. Chem., vol. 86, pp. 5257-5264, 2014. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54816 | - |
dc.description.abstract | 根據行政院衛生署統計,肺癌長年居於癌症十大死因之首,而現有之非侵入式檢測儀器如電腦斷層掃描(CT)與核磁共振儀(MRI)對於癌細胞影像的解析度不足,使得小於0.5公分的腫瘤難以從影像分辨因而難以在早期診斷出肺癌。因此,本研究希望以非侵入式之呼出氣體檢測方法檢測肺癌。研究發現,肺癌患者的呼出氣體中某些特定的揮發性有機化合物(Volatile organic compounds, VOCs)其含量會高於健康人體的基準值,因此可作為癌症檢測的生物標記(Biomarkers)。
本研究結合非微機電與微機電(MEMS)技術,開發出一台改良式攜帶型氣相層析儀並將其應用於肺癌生物標記之檢測。第一代改良式攜帶型氣相層析儀所設計出的體積為16×11×6 cm,此氣相層析儀以LabVIEW程式為基礎,搭配自行設計之電路架構作為各個元件及流道的控制系統;並使用傳統不銹鋼型前濃縮管、MEMS-based前濃縮管和MEMS-based分離管柱作為層析之關鍵元件,量測時使用一般空氣作為載流氣體,並用分子篩與活性碳過濾其水氣及有機物。本研究以奈米金單層膜保護團簇(Monolayer-protected gold nanoclusters, MPCs)作為感測材料塗佈於CMOS-based堆疊式電極結構感測器與MEMS-based平面式電極結構感測器,並搭配CMOS-based負迴授校正電路(Negative Feedback Calibration readout circuit, NFC)作為量測用之後端讀取電路,其測量結果較傳統分壓式讀取電路之感測靈敏度提升至少2倍。於氣相層析儀之量測上,兩種感測器皆成功在4分鐘內辨識由分離管柱分離出的7種有機揮發性氣體;而對於使用CMOS-based堆疊式電極結構-奈米金氣體感測器搭配傳統前濃縮管在採樣體積為300mL之條件下其偵測極限為12.9 ng(1,3,5-trimethylbenzene);使用 MEMS-based平面式電極結構-奈米金氣體感測器搭配MEMS-based前濃縮管在同條件下其偵測極限為137.1 ng(1,3,5-trimethylbenzene)。本研究開發的改良式攜帶型氣相層析儀為第一代原型機,其優點有分析迅速、體積小易於攜帶及再現性佳等優點;且未來可將CMOS-based氣體感測器與所有電路系統包括控制電路與後端讀取電路等整合於CMOS-based微晶片上,達成高整合性、高靈敏度、體積更小與功率損耗低之系統。 | zh_TW |
dc.description.abstract | According to the statistics from the Department of Health of Taiwan, lung cancer had been the leading causes of death for more than 5 years. With the existing non-invasive cancer diagnostic equipment, such as computed tomography (CT) and magnetic resonance imaging (MRI), it was very hard to identify an abnormal tissue or a tumor size smaller than 0.5 cm at the early stage of the cancer. By the previous research results, some volatile organic compounds (VOCs) from human exhaled air were approved as biomarkers for lung cancer. Therefore, the objective of the reported research was planned to use a novel non-invasive method to detect biomarkers of lung cancer.
In this research, an advanced portable micro gas chromatography (μGC) was developed to detect simulated lung cancer biomarkers by utilizing both non-MEMS and MEMS techniques. The size of the first generation μGC prototype was 16(L)×11(W)×6(H) cm, and it used custom-made LabVIEW program and electronic circuits to control the system. In addition, a conventional preconcentrator (PCT), a MEMS-based PCT and a MEMS-based column were employed as critical components in μGC. The CMOS-based and the MEMS-based VOC sensors were both coated with monolayer-protected gold nanoclusters (MPCs) as the sensing material. Both sensors were measured by utilizing the negative feedback calibration (NFC) readout circuit, and the sensitivity of sensors measured by NFC readout circuit was increased approximately two times higher compared to a conventional voltage divider. The μGC used the scrubbed air as the carrier gas, and vapor mixtures of seven compounds were successfully separated and identified by both two sensors in less than 4 minutes. The detection limit, in the volume of 300mL air sampling condition for 1,3,5-trimethylbenzene vapor, of the MEMS-based sensor connected with a conventional PCT was 137.1 ng while the detection limit of the CMOS-based sensor connected with MEMS-based PCT was 12.9 ng. This μGC was our first generation prototype, and it showed the features of portability, rapidness, and good repeatability. Ultimately, the CMOS-based sensor and entire electronic systems such as control circuits and NFC readout circuit could be integrated on the same micro-chip to further miniaturize the system and to reduce the power consumption in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T03:39:06Z (GMT). No. of bitstreams: 1 ntu-104-R01943152-1.pdf: 4851099 bytes, checksum: a839e56830ec81891beae51f2014c962 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 口試委員會審定書 I
誌謝 II 中文摘要 III ABSTRACT IV 目錄 VI 圖目錄 IX 表目錄 XIV 第1章 緒論 1 1.1 研究動機 1 1.2 氣相層析系統及其元件簡介 4 1.2.1 氣相層析系統簡介 4 1.2.2 前濃縮管原理與簡介 6 1.2.3 分離管柱原理與簡介 8 1.2.4 氣體感測器原理與簡介 10 1.3 氣相層析系統研究回顧 12 1.4 論文架構 21 第2章 氣相層析儀架構與設計 22 2.1 改良式攜帶型氣相層析儀原理與架構 22 2.2 氣相層析儀流道元件與架構 27 2.3 氣相層析儀電路元件與架構 32 2.3.1 繼電器控制電路 32 2.3.2 溫度控制回饋分壓電路 34 2.3.3 後端訊號讀取電路 38 2.4 氣體感測器結構原理與設計 42 2.4.1 奈米金單層膜保護團簇感測原理介紹 42 2.4.2 氣體感測器結構設計 43 第3章 氣相層析儀與揮發性有機化合物氣體微感測器之製作與量測 47 3.1 印刷電路板設計與製作 47 3.2 LabVIEW控制介面設計 48 3.3 氣體感測晶片封裝 49 3.4 奈米金單層膜保護團簇之塗佈 50 3.5 氣體感測器檢測方式 52 3.5.1 揮發性有機化合物氣體生成系統量測 52 3.5.2 標準品注入改良式攜帶型氣相層析儀量測 54 第4章 氣相層析儀量測結果與討論 57 4.1 氣體生成系統量測 57 4.1.1 不同種類氣體對感測器之反應 57 4.1.2 類比型負迴授校正電路之電容漏電現象 59 4.1.3 傳統分壓式與類比型負迴授校正讀取電路量測結果 61 4.2 標準品注入氣相層析儀量測 65 4.2.1氣相層析儀之功能測試 65 4.2.2 MEMS-based平面式電極感測器之標準氣體樣品量測 70 4.2.3 CMOS-based堆疊式電極感測器之標準氣體樣品量測 74 第5章 結論與未來展望 77 5.1 結論 77 5.2 未來展望 78 參考資料 79 | |
dc.language.iso | zh-TW | |
dc.title | 整合揮發性有機化合物氣體微感測器與改良式攜帶型氣相層析儀之研究 | zh_TW |
dc.title | Development of Portable Micro Gas Chromatography Utilizing Volatile Organic Compound Micro Sensors | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 呂家榮(Chia-Jung Lu),曾宇鳳(Yu-Feng Tseng),呂學士(Shey-Shi Lu) | |
dc.subject.keyword | 微型氣相層析儀,肺癌之生物標記,有機揮發性氣體,奈米金單層膜保護團簇,氣體微感測器, | zh_TW |
dc.subject.keyword | Micro gas chromatography,lung cancer biomarker,volatile organic compounds,monolayer-protected gold nanoclusters (MPCs),micro gas sensor, | en |
dc.relation.page | 83 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-02-24 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 電子工程學研究所 | zh_TW |
顯示於系所單位: | 電子工程學研究所 |
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